The Experts below are selected from a list of 365688 Experts worldwide ranked by ideXlab platform
Annemiek J.m. Cornelissen - One of the best experts on this subject based on the ideXlab platform.
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Tissue Growth pressure drives early blood flow in the chicken yolk sac
Developmental Dynamics, 2017Co-Authors: Raphaël Clément, Benjamin Mauroy, Annemiek J.m. CornelissenAbstract:BACKGROUND: Understanding how molecular and physical cues orchestrate vascular morphogenesis is a challenge for developmental biology. Only little attention has been paid to the impact of mechanical stress caused by Tissue Growth on early blood distribution. Here we study the peripheral accumulation of blood in the chicken embryonic yolk sac, which precedes sinus vein formation. RESULTS: We report that blood accumulation starts before heart-induced blood circulation. We hypothesized that the driving force for the primitive blood flow is a Growth-induced gradient of Tissue pressure in the yolk sac mesoderm. Therefore, we studied embryos in which heart development was arrested after 2 days of incubation, and found that yolk sac Growth and blood peripheral accumulation still occurred. This suggests that Tissue Growth is sufficient to initiate the flow and the formation of the sinus vein, whereas heart contractions are not required. We designed a simple mathematical model which makes explicit the Growth-induced pressure gradient and the subsequent blood accumulation, and show that Growth can indeed account for the observed blood accumulation. CONCLUSIONS: This study shows that Tissue Growth pressure can drive early blood flow, and suggests that the mechanical environment, beyond hemodynamics, can contribute to vascular morphogenesis. Developmental Dynamics 246:573-584, 2017. © 2017 Wiley Periodicals, Inc.
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Tissue Growth pressure drives early blood flow in the chicken yolk sac.
Developmental dynamics : an official publication of the American Association of Anatomists, 2017Co-Authors: Raphaël Clément, Benjamin Mauroy, Annemiek J.m. CornelissenAbstract:Background - Understanding how molecular and physical cues orchestrate vascular morphogenesis is a challenge for developmental biology. Only little attention has been paid to the impact of mechanical stress caused by Tissue Growth on early blood distribution. Here we study the peripheral accumulation of blood in the chicken embryonic yolk sac, which precedes sinus vein formation. Results - We report that blood accumulation starts prior to heart-induced blood circulation. We hypothesized that the driving force for the primitive blood flow is a Growth-induced gradient of Tissue pressure in the yolk sac mesoderm. Therefore, we studied embryos in which heart development was arrested after two days of incubation, and found that yolk sac Growth and blood peripheral accumulation still occurred. This suggests that Tissue Growth is sufficient to initiate the flow and the formation of the sinus vein, whereas heart contractions are not required. We designed a simple mathematical model which makes explicit the Growth-induced pressure gradient and the subsequent blood accumulation, and show that Growth can indeed account for the observed blood accumulation. Conclusions - This study shows that Tissue Growth pressure can drive early blood flow, and suggests that the mechanical environment, beyond hemodynamics, can contribute to vascular morphogenesis. This article is protected by copyright. All rights reserved.
Yavuz Kulaberoglu - One of the best experts on this subject based on the ideXlab platform.
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stable mob1 interaction with hippo mst is not essential for development and Tissue Growth control
Nature Communications, 2017Co-Authors: Yavuz Kulaberoglu, Maxine V Holder, Marta Gomez, Assefa B Shifa, M Mavis, Ahmad A D Sharif, C Lujan, Ewan St John Smith, I BjedovAbstract:The Hippo tumor suppressor pathway is essential for development and Tissue Growth control, encompassing a core cassette consisting of the Hippo (MST1/2), Warts (LATS1/2), and Tricornered (NDR1/2) kinases together with MOB1 as an important signaling adaptor. However, it remains unclear which regulatory interactions between MOB1 and the different Hippo core kinases coordinate development, Tissue Growth, and tumor suppression. Here, we report the crystal structure of the MOB1/NDR2 complex and define key MOB1 residues mediating MOB1’s differential binding to Hippo core kinases, thereby establishing MOB1 variants with selective loss-of-interaction. By studying these variants in human cancer cells and Drosophila, we uncovered that MOB1/Warts binding is essential for tumor suppression, Tissue Growth control, and development, while stable MOB1/Hippo binding is dispensable and MOB1/Trc binding alone is insufficient. Collectively, we decrypt molecularly, cell biologically, and genetically the importance of the diverse interactions of Hippo core kinases with the pivotal MOB1 signal transducer.
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Stable MOB1 interaction with Hippo/MST is not essential for development and Tissue Growth control.
Nature communications, 2017Co-Authors: Yavuz Kulaberoglu, Maxine V Holder, Marta Gomez, M Mavis, Ahmad A D Sharif, Kui Lin, Zhongchao Gai, Belul Assefa Shifa, Lily Hoa, C LujanAbstract:The Hippo tumor suppressor pathway is essential for development and Tissue Growth control, encompassing a core cassette consisting of the Hippo (MST1/2), Warts (LATS1/2), and Tricornered (NDR1/2) kinases together with MOB1 as an important signaling adaptor. However, it remains unclear which regulatory interactions between MOB1 and the different Hippo core kinases coordinate development, Tissue Growth, and tumor suppression. Here, we report the crystal structure of the MOB1/NDR2 complex and define key MOB1 residues mediating MOB1’s differential binding to Hippo core kinases, thereby establishing MOB1 variants with selective loss-of-interaction. By studying these variants in human cancer cells and Drosophila, we uncovered that MOB1/Warts binding is essential for tumor suppression, Tissue Growth control, and development, while stable MOB1/Hippo binding is dispensable and MOB1/Trc binding alone is insufficient. Collectively, we decrypt molecularly, cell biologically, and genetically the importance of the diverse interactions of Hippo core kinases with the pivotal MOB1 signal transducer.
Raphaël Clément - One of the best experts on this subject based on the ideXlab platform.
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Tissue Growth pressure drives early blood flow in the chicken yolk sac
Developmental Dynamics, 2017Co-Authors: Raphaël Clément, Benjamin Mauroy, Annemiek J.m. CornelissenAbstract:BACKGROUND: Understanding how molecular and physical cues orchestrate vascular morphogenesis is a challenge for developmental biology. Only little attention has been paid to the impact of mechanical stress caused by Tissue Growth on early blood distribution. Here we study the peripheral accumulation of blood in the chicken embryonic yolk sac, which precedes sinus vein formation. RESULTS: We report that blood accumulation starts before heart-induced blood circulation. We hypothesized that the driving force for the primitive blood flow is a Growth-induced gradient of Tissue pressure in the yolk sac mesoderm. Therefore, we studied embryos in which heart development was arrested after 2 days of incubation, and found that yolk sac Growth and blood peripheral accumulation still occurred. This suggests that Tissue Growth is sufficient to initiate the flow and the formation of the sinus vein, whereas heart contractions are not required. We designed a simple mathematical model which makes explicit the Growth-induced pressure gradient and the subsequent blood accumulation, and show that Growth can indeed account for the observed blood accumulation. CONCLUSIONS: This study shows that Tissue Growth pressure can drive early blood flow, and suggests that the mechanical environment, beyond hemodynamics, can contribute to vascular morphogenesis. Developmental Dynamics 246:573-584, 2017. © 2017 Wiley Periodicals, Inc.
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Tissue Growth pressure drives early blood flow in the chicken yolk sac.
Developmental dynamics : an official publication of the American Association of Anatomists, 2017Co-Authors: Raphaël Clément, Benjamin Mauroy, Annemiek J.m. CornelissenAbstract:Background - Understanding how molecular and physical cues orchestrate vascular morphogenesis is a challenge for developmental biology. Only little attention has been paid to the impact of mechanical stress caused by Tissue Growth on early blood distribution. Here we study the peripheral accumulation of blood in the chicken embryonic yolk sac, which precedes sinus vein formation. Results - We report that blood accumulation starts prior to heart-induced blood circulation. We hypothesized that the driving force for the primitive blood flow is a Growth-induced gradient of Tissue pressure in the yolk sac mesoderm. Therefore, we studied embryos in which heart development was arrested after two days of incubation, and found that yolk sac Growth and blood peripheral accumulation still occurred. This suggests that Tissue Growth is sufficient to initiate the flow and the formation of the sinus vein, whereas heart contractions are not required. We designed a simple mathematical model which makes explicit the Growth-induced pressure gradient and the subsequent blood accumulation, and show that Growth can indeed account for the observed blood accumulation. Conclusions - This study shows that Tissue Growth pressure can drive early blood flow, and suggests that the mechanical environment, beyond hemodynamics, can contribute to vascular morphogenesis. This article is protected by copyright. All rights reserved.
Stephen J. Ferguson - One of the best experts on this subject based on the ideXlab platform.
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Simulated Tissue Growth for 3D printed scaffolds.
Biomechanics and Modeling in Mechanobiology, 2018Co-Authors: Paul F. Egan, Kristina Shea, Stephen J. FergusonAbstract:Experiments have demonstrated biological Tissues grow by mechanically sensing their localized curvature, therefore making geometry a key consideration for Tissue scaffold design. We developed a simulation approach for modeling Tissue Growth on beam-based geometries of repeating unit cells, with four lattice topologies considered. In simulations, Tissue was seeded on surfaces with new Tissue growing in empty voxels with positive curvature. Growth was fastest on topologies with more beams per unit cell when unit cell volume/porosity was fixed, but fastest for topologies with fewer beams per unit cell when beam width/porosity was fixed. Tissue filled proportional to mean positive surface curvature per volume. Faster filling scaffolds had lower permeability, which is important to support nutrient transport, and highlights a need for tuning geometries appropriately for conflicting trade-offs. A balance among trade-offs was found for scaffolds with beam diameters of about $$300\,\upmu \hbox {m}$$ and 50% porosity, therefore providing the opportunity for further optimization based on criteria such as mechanical factors. Overall, these findings provide insight into how curvature-based Tissue Growth progresses in complex scaffold geometries, and a foundation for developing optimized scaffolds for clinical applications.
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Simulated Tissue Growth for 3D printed scaffolds.
Biomechanics and modeling in mechanobiology, 2018Co-Authors: Paul F. Egan, Kristina Shea, Stephen J. FergusonAbstract:Experiments have demonstrated biological Tissues grow by mechanically sensing their localized curvature, therefore making geometry a key consideration for Tissue scaffold design. We developed a simulation approach for modeling Tissue Growth on beam-based geometries of repeating unit cells, with four lattice topologies considered. In simulations, Tissue was seeded on surfaces with new Tissue growing in empty voxels with positive curvature. Growth was fastest on topologies with more beams per unit cell when unit cell volume/porosity was fixed, but fastest for topologies with fewer beams per unit cell when beam width/porosity was fixed. Tissue filled proportional to mean positive surface curvature per volume. Faster filling scaffolds had lower permeability, which is important to support nutrient transport, and highlights a need for tuning geometries appropriately for conflicting trade-offs. A balance among trade-offs was found for scaffolds with beam diameters of about [Formula: see text] and 50% porosity, therefore providing the opportunity for further optimization based on criteria such as mechanical factors. Overall, these findings provide insight into how curvature-based Tissue Growth progresses in complex scaffold geometries, and a foundation for developing optimized scaffolds for clinical applications.
C Lujan - One of the best experts on this subject based on the ideXlab platform.
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stable mob1 interaction with hippo mst is not essential for development and Tissue Growth control
Nature Communications, 2017Co-Authors: Yavuz Kulaberoglu, Maxine V Holder, Marta Gomez, Assefa B Shifa, M Mavis, Ahmad A D Sharif, C Lujan, Ewan St John Smith, I BjedovAbstract:The Hippo tumor suppressor pathway is essential for development and Tissue Growth control, encompassing a core cassette consisting of the Hippo (MST1/2), Warts (LATS1/2), and Tricornered (NDR1/2) kinases together with MOB1 as an important signaling adaptor. However, it remains unclear which regulatory interactions between MOB1 and the different Hippo core kinases coordinate development, Tissue Growth, and tumor suppression. Here, we report the crystal structure of the MOB1/NDR2 complex and define key MOB1 residues mediating MOB1’s differential binding to Hippo core kinases, thereby establishing MOB1 variants with selective loss-of-interaction. By studying these variants in human cancer cells and Drosophila, we uncovered that MOB1/Warts binding is essential for tumor suppression, Tissue Growth control, and development, while stable MOB1/Hippo binding is dispensable and MOB1/Trc binding alone is insufficient. Collectively, we decrypt molecularly, cell biologically, and genetically the importance of the diverse interactions of Hippo core kinases with the pivotal MOB1 signal transducer.
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Stable MOB1 interaction with Hippo/MST is not essential for development and Tissue Growth control.
Nature communications, 2017Co-Authors: Yavuz Kulaberoglu, Maxine V Holder, Marta Gomez, M Mavis, Ahmad A D Sharif, Kui Lin, Zhongchao Gai, Belul Assefa Shifa, Lily Hoa, C LujanAbstract:The Hippo tumor suppressor pathway is essential for development and Tissue Growth control, encompassing a core cassette consisting of the Hippo (MST1/2), Warts (LATS1/2), and Tricornered (NDR1/2) kinases together with MOB1 as an important signaling adaptor. However, it remains unclear which regulatory interactions between MOB1 and the different Hippo core kinases coordinate development, Tissue Growth, and tumor suppression. Here, we report the crystal structure of the MOB1/NDR2 complex and define key MOB1 residues mediating MOB1’s differential binding to Hippo core kinases, thereby establishing MOB1 variants with selective loss-of-interaction. By studying these variants in human cancer cells and Drosophila, we uncovered that MOB1/Warts binding is essential for tumor suppression, Tissue Growth control, and development, while stable MOB1/Hippo binding is dispensable and MOB1/Trc binding alone is insufficient. Collectively, we decrypt molecularly, cell biologically, and genetically the importance of the diverse interactions of Hippo core kinases with the pivotal MOB1 signal transducer.
