The Experts below are selected from a list of 2400 Experts worldwide ranked by ideXlab platform
Dazhi Wang - One of the best experts on this subject based on the ideXlab platform.
-
novel roles of gata4 6 in the postnatal heart identified through temporally controlled cardiomyocyte specific gene inactivation by adeno associated virus delivery of cre recombinase
PLOS ONE, 2015Co-Authors: Terence Prendiville, Pingzhu Zhou, Sean M Stevens, Aibin He, Nathan J Vandusen, Jinghai Chen, Li Zhong, Dazhi WangAbstract:GATA4 and GATA6 are central cardiac transcriptional regulators. The postnatal, stage-specific function of the cardiac transcription factors GATA4 and GATA6 have not been evaluated. In part, this is because current Cre-loxP approaches to cardiac gene inactivation require time consuming and costly breeding of Cre-expressing and “floxed” mouse lines, often with limited control of the extent or timing of gene inactivation. We investigated the stage-specific functions of GATA4 and GATA6 in the postnatal heart by using adeno-associated virus serotype 9 to control the timing and extent of gene inactivation by Cre. Systemic delivery of recombinant, adeno-associated virus 9 (AAV9) expressing Cre from the cardiac specific TNNT2 promoter was well tolerated and selectively and efficiently recombined floxed target genes in cardiomyocytes. AAV9:TNNT2-Cre efficiently inactivated Gata4 and Gata6. Neonatal Gata4/6 inactivation caused severe, rapidly lethal systolic heart failure. In contrast, Gata4/6 inactivation in adult heart caused only mild systolic dysfunction but severe diastolic dysfunction. Reducing the dose of AAV9:TNNT2-Cre generated mosaics in which scattered cardiomyocytes lacked Gata4/6. This mosaic knockout revealed that Gata4/6 are required cell autonomously for physiological cardiomyocyte growth. Our results define novel roles of GATA4 and GATA6 in the neonatal and adult heart. Furthermore, our data demonstrate that evaluation of gene function hinges on controlling the timing and extent of gene inactivation. AAV9:TNNT2-Cre is a powerful tool for controlling these parameters.
-
Novel Roles of GATA4/6 in the Postnatal Heart Identified through Temporally Controlled, Cardiomyocyte-Specific Gene Inactivation by Adeno-Associated Virus Delivery of Cre Recombinase
PLOS ONE, 2015Co-Authors: Terence Prendiville, Pingzhu Zhou, Sean M Stevens, Aibin He, Nathan J Vandusen, Jinghai Chen, Li Zhong, Dazhi WangAbstract:GATA4 and GATA6 are central cardiac transcriptional regulators. The postnatal, stage-specific function of the cardiac transcription factors GATA4 and GATA6 have not been evaluated. In part, this is because current Cre-loxP approaches to cardiac gene inactivation require time consuming and costly breeding of Cre-expressing and “floxed” mouse lines, often with limited control of the extent or timing of gene inactivation. We investigated the stage-specific functions of GATA4 and GATA6 in the postnatal heart by using adeno-associated virus serotype 9 to control the timing and extent of gene inactivation by Cre. Systemic delivery of recombinant, adeno-associated virus 9 (AAV9) expressing Cre from the cardiac specific TNNT2 promoter was well tolerated and selectively and efficiently recombined floxed target genes in cardiomyocytes. AAV9:TNNT2-Cre efficiently inactivated Gata4 and Gata6. Neonatal Gata4/6 inactivation caused severe, rapidly lethal systolic heart failure. In contrast, Gata4/6 inactivation in adult heart caused only mild systolic dysfunction but severe diastolic dysfunction. Reducing the dose of AAV9:TNNT2-Cre generated mosaics in which scattered cardiomyocytes lacked Gata4/6. This mosaic knockout revealed that Gata4/6 are required cell autonomously for physiological cardiomyocyte growth. Our results define novel roles of GATA4 and GATA6 in the neonatal and adult heart. Furthermore, our data demonstrate that evaluation of gene function hinges on controlling the timing and extent of gene inactivation. AAV9:TNNT2-Cre is a powerful tool for controlling these parameters.
Vadlamudi Raghavendra Rao - One of the best experts on this subject based on the ideXlab platform.
-
TNNT2 5 bp deletion sedentary lifestyle abdominal adiposity accentuate the phenotypic severity in hypertrophic cardiomyopathy patients
Meta Gene, 2019Co-Authors: Amitabh Biswas, Mitali Kapoor, Soumi Das, Sandeep Seth, Balram Bhargava, Vadlamudi Raghavendra RaoAbstract:Abstract Hypertrophic cardiomyopathy (HCM) is a common cardiac genetic disorder having phenotypic heterogeneity, which can be attributed to interaction of certain environmental risk factors and lifestyle modifications. HCM cases and clinically evaluated controls were evaluated for lifestyle, anthropometric, physiological and biochemical variables, followed by screening of HCM associated genetic variations (5bpDel in TNNT2, 25bpDel in MYBPC3, 287bpDel in ACE and T-786C in eNOS). Sedentary lifestyle, waist-hip ratio, total serum cholesterol and triglycerides along with 5bpDel in TNNT2 gene were found to be significantly associated with the disease condition (p
-
TNNT2 5 bp deletion, sedentary lifestyle & abdominal adiposity accentuate the phenotypic severity in hypertrophic cardiomyopathy patients
Meta Gene, 2019Co-Authors: Amitabh Biswas, Mitali Kapoor, Soumi Das, Sandeep Seth, Balram Bhargava, Vadlamudi Raghavendra RaoAbstract:Abstract Hypertrophic cardiomyopathy (HCM) is a common cardiac genetic disorder having phenotypic heterogeneity, which can be attributed to interaction of certain environmental risk factors and lifestyle modifications. HCM cases and clinically evaluated controls were evaluated for lifestyle, anthropometric, physiological and biochemical variables, followed by screening of HCM associated genetic variations (5bpDel in TNNT2, 25bpDel in MYBPC3, 287bpDel in ACE and T-786C in eNOS). Sedentary lifestyle, waist-hip ratio, total serum cholesterol and triglycerides along with 5bpDel in TNNT2 gene were found to be significantly associated with the disease condition (p
Amitabh Biswas - One of the best experts on this subject based on the ideXlab platform.
-
TNNT2 5 bp deletion sedentary lifestyle abdominal adiposity accentuate the phenotypic severity in hypertrophic cardiomyopathy patients
Meta Gene, 2019Co-Authors: Amitabh Biswas, Mitali Kapoor, Soumi Das, Sandeep Seth, Balram Bhargava, Vadlamudi Raghavendra RaoAbstract:Abstract Hypertrophic cardiomyopathy (HCM) is a common cardiac genetic disorder having phenotypic heterogeneity, which can be attributed to interaction of certain environmental risk factors and lifestyle modifications. HCM cases and clinically evaluated controls were evaluated for lifestyle, anthropometric, physiological and biochemical variables, followed by screening of HCM associated genetic variations (5bpDel in TNNT2, 25bpDel in MYBPC3, 287bpDel in ACE and T-786C in eNOS). Sedentary lifestyle, waist-hip ratio, total serum cholesterol and triglycerides along with 5bpDel in TNNT2 gene were found to be significantly associated with the disease condition (p
-
TNNT2 5 bp deletion, sedentary lifestyle & abdominal adiposity accentuate the phenotypic severity in hypertrophic cardiomyopathy patients
Meta Gene, 2019Co-Authors: Amitabh Biswas, Mitali Kapoor, Soumi Das, Sandeep Seth, Balram Bhargava, Vadlamudi Raghavendra RaoAbstract:Abstract Hypertrophic cardiomyopathy (HCM) is a common cardiac genetic disorder having phenotypic heterogeneity, which can be attributed to interaction of certain environmental risk factors and lifestyle modifications. HCM cases and clinically evaluated controls were evaluated for lifestyle, anthropometric, physiological and biochemical variables, followed by screening of HCM associated genetic variations (5bpDel in TNNT2, 25bpDel in MYBPC3, 287bpDel in ACE and T-786C in eNOS). Sedentary lifestyle, waist-hip ratio, total serum cholesterol and triglycerides along with 5bpDel in TNNT2 gene were found to be significantly associated with the disease condition (p
Aibin He - One of the best experts on this subject based on the ideXlab platform.
-
novel roles of gata4 6 in the postnatal heart identified through temporally controlled cardiomyocyte specific gene inactivation by adeno associated virus delivery of cre recombinase
PLOS ONE, 2015Co-Authors: Terence Prendiville, Pingzhu Zhou, Sean M Stevens, Aibin He, Nathan J Vandusen, Jinghai Chen, Li Zhong, Dazhi WangAbstract:GATA4 and GATA6 are central cardiac transcriptional regulators. The postnatal, stage-specific function of the cardiac transcription factors GATA4 and GATA6 have not been evaluated. In part, this is because current Cre-loxP approaches to cardiac gene inactivation require time consuming and costly breeding of Cre-expressing and “floxed” mouse lines, often with limited control of the extent or timing of gene inactivation. We investigated the stage-specific functions of GATA4 and GATA6 in the postnatal heart by using adeno-associated virus serotype 9 to control the timing and extent of gene inactivation by Cre. Systemic delivery of recombinant, adeno-associated virus 9 (AAV9) expressing Cre from the cardiac specific TNNT2 promoter was well tolerated and selectively and efficiently recombined floxed target genes in cardiomyocytes. AAV9:TNNT2-Cre efficiently inactivated Gata4 and Gata6. Neonatal Gata4/6 inactivation caused severe, rapidly lethal systolic heart failure. In contrast, Gata4/6 inactivation in adult heart caused only mild systolic dysfunction but severe diastolic dysfunction. Reducing the dose of AAV9:TNNT2-Cre generated mosaics in which scattered cardiomyocytes lacked Gata4/6. This mosaic knockout revealed that Gata4/6 are required cell autonomously for physiological cardiomyocyte growth. Our results define novel roles of GATA4 and GATA6 in the neonatal and adult heart. Furthermore, our data demonstrate that evaluation of gene function hinges on controlling the timing and extent of gene inactivation. AAV9:TNNT2-Cre is a powerful tool for controlling these parameters.
-
Novel Roles of GATA4/6 in the Postnatal Heart Identified through Temporally Controlled, Cardiomyocyte-Specific Gene Inactivation by Adeno-Associated Virus Delivery of Cre Recombinase
PLOS ONE, 2015Co-Authors: Terence Prendiville, Pingzhu Zhou, Sean M Stevens, Aibin He, Nathan J Vandusen, Jinghai Chen, Li Zhong, Dazhi WangAbstract:GATA4 and GATA6 are central cardiac transcriptional regulators. The postnatal, stage-specific function of the cardiac transcription factors GATA4 and GATA6 have not been evaluated. In part, this is because current Cre-loxP approaches to cardiac gene inactivation require time consuming and costly breeding of Cre-expressing and “floxed” mouse lines, often with limited control of the extent or timing of gene inactivation. We investigated the stage-specific functions of GATA4 and GATA6 in the postnatal heart by using adeno-associated virus serotype 9 to control the timing and extent of gene inactivation by Cre. Systemic delivery of recombinant, adeno-associated virus 9 (AAV9) expressing Cre from the cardiac specific TNNT2 promoter was well tolerated and selectively and efficiently recombined floxed target genes in cardiomyocytes. AAV9:TNNT2-Cre efficiently inactivated Gata4 and Gata6. Neonatal Gata4/6 inactivation caused severe, rapidly lethal systolic heart failure. In contrast, Gata4/6 inactivation in adult heart caused only mild systolic dysfunction but severe diastolic dysfunction. Reducing the dose of AAV9:TNNT2-Cre generated mosaics in which scattered cardiomyocytes lacked Gata4/6. This mosaic knockout revealed that Gata4/6 are required cell autonomously for physiological cardiomyocyte growth. Our results define novel roles of GATA4 and GATA6 in the neonatal and adult heart. Furthermore, our data demonstrate that evaluation of gene function hinges on controlling the timing and extent of gene inactivation. AAV9:TNNT2-Cre is a powerful tool for controlling these parameters.
Terence Prendiville - One of the best experts on this subject based on the ideXlab platform.
-
novel roles of gata4 6 in the postnatal heart identified through temporally controlled cardiomyocyte specific gene inactivation by adeno associated virus delivery of cre recombinase
PLOS ONE, 2015Co-Authors: Terence Prendiville, Pingzhu Zhou, Sean M Stevens, Aibin He, Nathan J Vandusen, Jinghai Chen, Li Zhong, Dazhi WangAbstract:GATA4 and GATA6 are central cardiac transcriptional regulators. The postnatal, stage-specific function of the cardiac transcription factors GATA4 and GATA6 have not been evaluated. In part, this is because current Cre-loxP approaches to cardiac gene inactivation require time consuming and costly breeding of Cre-expressing and “floxed” mouse lines, often with limited control of the extent or timing of gene inactivation. We investigated the stage-specific functions of GATA4 and GATA6 in the postnatal heart by using adeno-associated virus serotype 9 to control the timing and extent of gene inactivation by Cre. Systemic delivery of recombinant, adeno-associated virus 9 (AAV9) expressing Cre from the cardiac specific TNNT2 promoter was well tolerated and selectively and efficiently recombined floxed target genes in cardiomyocytes. AAV9:TNNT2-Cre efficiently inactivated Gata4 and Gata6. Neonatal Gata4/6 inactivation caused severe, rapidly lethal systolic heart failure. In contrast, Gata4/6 inactivation in adult heart caused only mild systolic dysfunction but severe diastolic dysfunction. Reducing the dose of AAV9:TNNT2-Cre generated mosaics in which scattered cardiomyocytes lacked Gata4/6. This mosaic knockout revealed that Gata4/6 are required cell autonomously for physiological cardiomyocyte growth. Our results define novel roles of GATA4 and GATA6 in the neonatal and adult heart. Furthermore, our data demonstrate that evaluation of gene function hinges on controlling the timing and extent of gene inactivation. AAV9:TNNT2-Cre is a powerful tool for controlling these parameters.
-
Novel Roles of GATA4/6 in the Postnatal Heart Identified through Temporally Controlled, Cardiomyocyte-Specific Gene Inactivation by Adeno-Associated Virus Delivery of Cre Recombinase
PLOS ONE, 2015Co-Authors: Terence Prendiville, Pingzhu Zhou, Sean M Stevens, Aibin He, Nathan J Vandusen, Jinghai Chen, Li Zhong, Dazhi WangAbstract:GATA4 and GATA6 are central cardiac transcriptional regulators. The postnatal, stage-specific function of the cardiac transcription factors GATA4 and GATA6 have not been evaluated. In part, this is because current Cre-loxP approaches to cardiac gene inactivation require time consuming and costly breeding of Cre-expressing and “floxed” mouse lines, often with limited control of the extent or timing of gene inactivation. We investigated the stage-specific functions of GATA4 and GATA6 in the postnatal heart by using adeno-associated virus serotype 9 to control the timing and extent of gene inactivation by Cre. Systemic delivery of recombinant, adeno-associated virus 9 (AAV9) expressing Cre from the cardiac specific TNNT2 promoter was well tolerated and selectively and efficiently recombined floxed target genes in cardiomyocytes. AAV9:TNNT2-Cre efficiently inactivated Gata4 and Gata6. Neonatal Gata4/6 inactivation caused severe, rapidly lethal systolic heart failure. In contrast, Gata4/6 inactivation in adult heart caused only mild systolic dysfunction but severe diastolic dysfunction. Reducing the dose of AAV9:TNNT2-Cre generated mosaics in which scattered cardiomyocytes lacked Gata4/6. This mosaic knockout revealed that Gata4/6 are required cell autonomously for physiological cardiomyocyte growth. Our results define novel roles of GATA4 and GATA6 in the neonatal and adult heart. Furthermore, our data demonstrate that evaluation of gene function hinges on controlling the timing and extent of gene inactivation. AAV9:TNNT2-Cre is a powerful tool for controlling these parameters.