The Experts below are selected from a list of 20025 Experts worldwide ranked by ideXlab platform
Alexander Mcpherson - One of the best experts on this subject based on the ideXlab platform.
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double helical rna in satellite Tobacco Mosaic Virus
Nature, 1993Co-Authors: Steven B Larson, Stanley Koszelak, John Day, Aaron Greenwood, Allan J Dodds, Alexander McphersonAbstract:Satellite Tobacco Mosaic Virus (STMV) is the spherical satellite to an obligatory rod-shaped helper Tobacco Mosaic Virus (TMV), which is required for replication. STMV has 60 protein subunits of M(r) 17,500 on a T = 1 icosahedral capsid containing a single-stranded RNA genome of 1,059 bases. STMV appears similar to another Virus, STNV, but is approximately 20 per cent smaller. It shows no amino-acid homology or immunological cross-reactivity with either STNV or its host TMV. Here we report the X-ray crystal structure of STMV, which shows that the coat protein of STMV contains a 'Swiss roll' beta-barrel. An amino-terminal strand extends more than 60A and is primarily responsible for quaternary interactions. Each capsid dimer is associated with a segment of genomic RNA double helix comprising seven base pairs. The dyad of each protein dimer is coincident with that of the central base pair of the associated RNA segment whose helix axis is directed along an icosahedral edge. Protein-nucleic acid interactions are extensive. The RNA helices, which have additional stacked bases at their 3' termini, differ significantly from canonical nucleic acid helical forms.
R.c. Wilhelm - One of the best experts on this subject based on the ideXlab platform.
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Reaction differences between Tobacco Mosaic Virus and its free ribonucleic acid with nitrous acid
Biochimica et Biophysica Acta, 2003Co-Authors: H. Schuster, R.c. WilhelmAbstract:Abstract During the treatment of Tobacco Mosaic Virus ribonucleic acid with nitrous acid at pH 4.2 and 5.0 the amino bases adenine, guanine and cytosine react with similar velocities. However, not all alterations of guanine by nitrous acid lead to the formation of xanthine. During treatment of intact Tobacco Mosaic Virus with nitrous acid, adenine and cytosine are deaminated, whereas guanine is not attacked. The importance of these findings for the mutagenic action of nitrous acid on Tobacco Mosaic Virus is discussed.
Steven B Larson - One of the best experts on this subject based on the ideXlab platform.
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double helical rna in satellite Tobacco Mosaic Virus
Nature, 1993Co-Authors: Steven B Larson, Stanley Koszelak, John Day, Aaron Greenwood, Allan J Dodds, Alexander McphersonAbstract:Satellite Tobacco Mosaic Virus (STMV) is the spherical satellite to an obligatory rod-shaped helper Tobacco Mosaic Virus (TMV), which is required for replication. STMV has 60 protein subunits of M(r) 17,500 on a T = 1 icosahedral capsid containing a single-stranded RNA genome of 1,059 bases. STMV appears similar to another Virus, STNV, but is approximately 20 per cent smaller. It shows no amino-acid homology or immunological cross-reactivity with either STNV or its host TMV. Here we report the X-ray crystal structure of STMV, which shows that the coat protein of STMV contains a 'Swiss roll' beta-barrel. An amino-terminal strand extends more than 60A and is primarily responsible for quaternary interactions. Each capsid dimer is associated with a segment of genomic RNA double helix comprising seven base pairs. The dyad of each protein dimer is coincident with that of the central base pair of the associated RNA segment whose helix axis is directed along an icosahedral edge. Protein-nucleic acid interactions are extensive. The RNA helices, which have additional stacked bases at their 3' termini, differ significantly from canonical nucleic acid helical forms.
H. Schuster - One of the best experts on this subject based on the ideXlab platform.
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Reaction differences between Tobacco Mosaic Virus and its free ribonucleic acid with nitrous acid
Biochimica et Biophysica Acta, 2003Co-Authors: H. Schuster, R.c. WilhelmAbstract:Abstract During the treatment of Tobacco Mosaic Virus ribonucleic acid with nitrous acid at pH 4.2 and 5.0 the amino bases adenine, guanine and cytosine react with similar velocities. However, not all alterations of guanine by nitrous acid lead to the formation of xanthine. During treatment of intact Tobacco Mosaic Virus with nitrous acid, adenine and cytosine are deaminated, whereas guanine is not attacked. The importance of these findings for the mutagenic action of nitrous acid on Tobacco Mosaic Virus is discussed.
Michael Metzlaff - One of the best experts on this subject based on the ideXlab platform.
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Current Protocols in Microbiology - Using satellite Tobacco Mosaic Virus vectors for gene silencing.
Current protocols in microbiology, 2005Co-Authors: Veronique Gossele, Michael MetzlaffAbstract:This unit describes the use of satellite Tobacco Mosaic Virus (STMV) vectors in combination with native TMV particles for inducing transient gene silencing in Tobacco plants. Target gene fragment selection and insertion, Virus delivery procedures, and phenotype screening of silenced plants are described in detail. All critical parameters for Tobacco plant cultivation, Virus infection, and RNA silencing efficiency are discussed. Keywords: RNA silencing; viral vectors; satellite Virus–induced silencing system; Nicotiana tabacum; Tobacco Mosaic Virus; plant gene function discovery
