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Briedis Vitalis - One of the best experts on this subject based on the ideXlab platform.

  • Study of Tolnaftate release from fatty acids containing ointment and penetration into human skin ex vivo
    2020
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Rimdeika Rytis, Briedis Vitalis
    Abstract:

    Five fatty acids (oleic, linoleic, myristic, lauric and capric) were incorporated in 10% (w/w) into ointment formulation and their influence on lipophilic model drug Tolnaftate release in vitro and enhancing effect on Tolnaftate penetration into epidermis and dermis of human skin ex vivo were investigated. The prepared ointments were tested for homogeneity, pH and rheological properties. In vitro release studies and ex vivo skin penetration experiments were carried out using Hanson and Bronaugh-type flow-through diffusion cells, respectively. Tolnaftate cumulative amount liberated from semisolids was assayed using UV-Vis spectrophotometer. After in vitro skin penetration studies, appropriately extracted human skin layers were analyzed for Tolnaftate content using a validated HPLC method. Statistical analysis revealed that release rate of Tolnaftate from control ointment and ointments with fatty acids was not significantly different and only 7.34ñ8.98% of drug was liberated into an acceptor medium after 6 h. Tolnaftate amount penetrating into 1 cm2 of epidermis from ointments containing oleic, linoleic, myristic and lauric acids was significantly greater (p < 0.05) than from the control ointment. Penetration enhancing ratios for these fatty acids for Tolnaftate penetration into epidermis ranged from 1.48 to 1.75. In conclusion, fatty acids did not increase the liberation of Tolnaftate from ointment formulation, but demonstrated their enhancing effect on Tolnaftate penetration into human epidermis in vitro. Results from in vitro release experiments do not suit for prediction of the situation in the skin in vitro, if chemical penetration enhancers are incorporated into the ointment formulationBiochemijos katedraGamtos mokslų fakultetasLietuvos Sveikatos mokslų universiteto ligoninėLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Study of Tolnaftate release from fatty acids containing ointment and penetration into human skin ex vivo
    2020
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Rimdeika Rytis, Briedis Vitalis
    Abstract:

    Five fatty acids (oleic, linoleic, myristic, lauric and capric) were incorporated in 10% (w/w) into ointment formulation and their influence on lipophilic model drug Tolnaftate release in vitro and enhancing effect on Tolnaftate penetration into epidermis and dermis of human skin ex vivo were investigated. The prepared ointments were tested for homogeneity, pH and rheological properties. In vitro release studies and ex vivo skin penetration experiments were carried out using Hanson and Bronaugh-type flow-through diffusion cells, respectively. Tolnaftate cumulative amount liberated from semisolids was assayed using UV-Vis spectrophotometer. After in vitro skin penetration studies, appropriately extracted human skin layers were analyzed for Tolnaftate content using a validated HPLC method. Statistical analysis revealed that release rate of Tolnaftate from control ointment and ointments with fatty acids was not significantly different and only 7.34ñ8.98% of drug was liberated into an acceptor medium after 6 h. Tolnaftate amount penetrating into 1 cm2 of epidermis from ointments containing oleic, linoleic, myristic and lauric acids was significantly greater (p < 0.05) than from the control ointment. Penetration enhancing ratios for these fatty acids for Tolnaftate penetration into epidermis ranged from 1.48 to 1.75. In conclusion, fatty acids did not increase the liberation of Tolnaftate from ointment formulation, but demonstrated their enhancing effect on Tolnaftate penetration into human epidermis in vitro. Results from in vitro release experiments do not suit for prediction of the situation in the skin in vitro, if chemical penetration enhancers are incorporated into the ointment formulationBiochemijos katedraLietuvos Sveikatos mokslų universiteto ligoninėLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Assay of Tolnaftate in human skin samples after in vitro penetration studies using high performance liquid chromatography
    2020
    Co-Authors: Kežutytė Toma, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tolnaftate, an antifungal of thiocarbamate class, is used topically in 1% formulations. Its penetration into skin layers is a prerequisite for Tolnaftate action against dermatophytes. The aim of this work was to optimize and validate a simple, rapid, accurate and reproducible procedure for Tolnaftate assay in human skin samples and to apply this procedure for in vitro Tolnaftate penetration studies. High performance liquid chromatography (HPLC) method with UV detection was used to validate Tolnaftate assay for linearity, specificity, accuracy, precision, limit of quantitation, limit of detection, drug extraction recovery and stability in skin extracts. In vitro Tolnaftate penetration studies were carried out using flow-through diffusion cells, mounted with human skin. Epidermis and dermis, separated by heat-separation method, were extracted using ultrasonication in methanol. Linear range of the analytical procedure was within 0.6ñ100 μg/mL. The assay was specific, accurate (within-day and between-day recovery values were 98.2ñ104.2% and 98.7ñ101.4%, respectively) and precise (within-day and between-day imprecision was = 3.8%). Mean extraction recoveries of Tolnaftate from epidermis and dermis were satisfactory and reaching 90%. In vitro skin penetration studies revealed that after application of 1% (w/w) Tolnaftate solution in polyethylene glycol 400 for 24 hours, the mean amount of Tolnaftate penetrating into the epidermis and dermis was 2.60 ± 0.28 μg/cm2 and 0.92 ± 0.12 μg/cm2, respectively. A validated reliable HPLC method could be recommended for biopharmaceutical evaluation of Tolnaftate preparations and studies of pharmacokinetics in human skin after in vitro penetration studiesBiochemijos katedraLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Assay of Tolnaftate in human skin samples after in vitro penetration studies using high performance liquid chromatography
    2020
    Co-Authors: Kežutytė Toma, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tolnaftate, an antifungal of thiocarbamate class, is used topically in 1% formulations. Its penetration into skin layers is a prerequisite for Tolnaftate action against dermatophytes. The aim of this work was to optimize and validate a simple, rapid, accurate and reproducible procedure for Tolnaftate assay in human skin samples and to apply this procedure for in vitro Tolnaftate penetration studies. High performance liquid chromatography (HPLC) method with UV detection was used to validate Tolnaftate assay for linearity, specificity, accuracy, precision, limit of quantitation, limit of detection, drug extraction recovery and stability in skin extracts. In vitro Tolnaftate penetration studies were carried out using flow-through diffusion cells, mounted with human skin. Epidermis and dermis, separated by heat-separation method, were extracted using ultrasonication in methanol. Linear range of the analytical procedure was within 0.6ñ100 μg/mL. The assay was specific, accurate (within-day and between-day recovery values were 98.2ñ104.2% and 98.7ñ101.4%, respectively) and precise (within-day and between-day imprecision was = 3.8%). Mean extraction recoveries of Tolnaftate from epidermis and dermis were satisfactory and reaching 90%. In vitro skin penetration studies revealed that after application of 1% (w/w) Tolnaftate solution in polyethylene glycol 400 for 24 hours, the mean amount of Tolnaftate penetrating into the epidermis and dermis was 2.60 ± 0.28 μg/cm2 and 0.92 ± 0.12 μg/cm2, respectively. A validated reliable HPLC method could be recommended for biopharmaceutical evaluation of Tolnaftate preparations and studies of pharmacokinetics in human skin after in vitro penetration studiesBiochemijos katedraGamtos mokslų fakultetasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Tolnaftate penetration into human skin ex vivo from solution and ointment formulations containing chemical penetration enchancers
    2015
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tyrinėtas į tirpalą ir tepalą įvestų oleino, linolo, lauro ir kapro riebiųjų rūgščių skatinantis poveikis tolnaftato skverbimuisi į žmogaus odą ex vivo. Riebiosios rūgštys priklauso lipofiliniams cheminiams skvarbos skatintojams, kurie gali sutrikdyti stratum corneum lipidų išsidėstymą ir paskatinti hidrofobinių vaistų, tokių kaip tolnaftatas, sverbimąsi. Tolnaftato 1% (m/m) tirpalas gautas ištirpinus jį polietilenglikolyje (PEG) 400, o 1% (m/m) tepalas - įvedus tiirpalą į baltojo vazelino, bevandenio lanolino ir PEG 1500 lydinį. Į abi vaisto formas pridėta po 10% (m/m) tyrinėtų riebiųjų rūgščių. [...]Oleic, linoleic, lauric and capric fatty acids were incorporated in 10% (w/w) into solution and ointment formulations and their enhancing effect on Tolnaftate penetration into human skin ex vivo was investigated. Fatty acids belong to the lipophilic chemical penetration enhancers (CPEs), which might alter stratum corneum lipid organization and increase the penetration of hydrophobic active drugs, such as Tolnaftate. Tolnaftate in 1% (w/w) was dissolved in polyethylene glycol (PEG) 400 or incorporated into an ointment, composed of white vaseline, anhydrous lanolin, PEG 400 and PEG 1500. 10% (w/w) of each fatty acid were added to both formulations. [...]Biochemijos katedraLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Kežutytė Toma - One of the best experts on this subject based on the ideXlab platform.

  • Study of Tolnaftate release from fatty acids containing ointment and penetration into human skin ex vivo
    2020
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Rimdeika Rytis, Briedis Vitalis
    Abstract:

    Five fatty acids (oleic, linoleic, myristic, lauric and capric) were incorporated in 10% (w/w) into ointment formulation and their influence on lipophilic model drug Tolnaftate release in vitro and enhancing effect on Tolnaftate penetration into epidermis and dermis of human skin ex vivo were investigated. The prepared ointments were tested for homogeneity, pH and rheological properties. In vitro release studies and ex vivo skin penetration experiments were carried out using Hanson and Bronaugh-type flow-through diffusion cells, respectively. Tolnaftate cumulative amount liberated from semisolids was assayed using UV-Vis spectrophotometer. After in vitro skin penetration studies, appropriately extracted human skin layers were analyzed for Tolnaftate content using a validated HPLC method. Statistical analysis revealed that release rate of Tolnaftate from control ointment and ointments with fatty acids was not significantly different and only 7.34ñ8.98% of drug was liberated into an acceptor medium after 6 h. Tolnaftate amount penetrating into 1 cm2 of epidermis from ointments containing oleic, linoleic, myristic and lauric acids was significantly greater (p < 0.05) than from the control ointment. Penetration enhancing ratios for these fatty acids for Tolnaftate penetration into epidermis ranged from 1.48 to 1.75. In conclusion, fatty acids did not increase the liberation of Tolnaftate from ointment formulation, but demonstrated their enhancing effect on Tolnaftate penetration into human epidermis in vitro. Results from in vitro release experiments do not suit for prediction of the situation in the skin in vitro, if chemical penetration enhancers are incorporated into the ointment formulationBiochemijos katedraGamtos mokslų fakultetasLietuvos Sveikatos mokslų universiteto ligoninėLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Study of Tolnaftate release from fatty acids containing ointment and penetration into human skin ex vivo
    2020
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Rimdeika Rytis, Briedis Vitalis
    Abstract:

    Five fatty acids (oleic, linoleic, myristic, lauric and capric) were incorporated in 10% (w/w) into ointment formulation and their influence on lipophilic model drug Tolnaftate release in vitro and enhancing effect on Tolnaftate penetration into epidermis and dermis of human skin ex vivo were investigated. The prepared ointments were tested for homogeneity, pH and rheological properties. In vitro release studies and ex vivo skin penetration experiments were carried out using Hanson and Bronaugh-type flow-through diffusion cells, respectively. Tolnaftate cumulative amount liberated from semisolids was assayed using UV-Vis spectrophotometer. After in vitro skin penetration studies, appropriately extracted human skin layers were analyzed for Tolnaftate content using a validated HPLC method. Statistical analysis revealed that release rate of Tolnaftate from control ointment and ointments with fatty acids was not significantly different and only 7.34ñ8.98% of drug was liberated into an acceptor medium after 6 h. Tolnaftate amount penetrating into 1 cm2 of epidermis from ointments containing oleic, linoleic, myristic and lauric acids was significantly greater (p < 0.05) than from the control ointment. Penetration enhancing ratios for these fatty acids for Tolnaftate penetration into epidermis ranged from 1.48 to 1.75. In conclusion, fatty acids did not increase the liberation of Tolnaftate from ointment formulation, but demonstrated their enhancing effect on Tolnaftate penetration into human epidermis in vitro. Results from in vitro release experiments do not suit for prediction of the situation in the skin in vitro, if chemical penetration enhancers are incorporated into the ointment formulationBiochemijos katedraLietuvos Sveikatos mokslų universiteto ligoninėLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Assay of Tolnaftate in human skin samples after in vitro penetration studies using high performance liquid chromatography
    2020
    Co-Authors: Kežutytė Toma, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tolnaftate, an antifungal of thiocarbamate class, is used topically in 1% formulations. Its penetration into skin layers is a prerequisite for Tolnaftate action against dermatophytes. The aim of this work was to optimize and validate a simple, rapid, accurate and reproducible procedure for Tolnaftate assay in human skin samples and to apply this procedure for in vitro Tolnaftate penetration studies. High performance liquid chromatography (HPLC) method with UV detection was used to validate Tolnaftate assay for linearity, specificity, accuracy, precision, limit of quantitation, limit of detection, drug extraction recovery and stability in skin extracts. In vitro Tolnaftate penetration studies were carried out using flow-through diffusion cells, mounted with human skin. Epidermis and dermis, separated by heat-separation method, were extracted using ultrasonication in methanol. Linear range of the analytical procedure was within 0.6ñ100 μg/mL. The assay was specific, accurate (within-day and between-day recovery values were 98.2ñ104.2% and 98.7ñ101.4%, respectively) and precise (within-day and between-day imprecision was = 3.8%). Mean extraction recoveries of Tolnaftate from epidermis and dermis were satisfactory and reaching 90%. In vitro skin penetration studies revealed that after application of 1% (w/w) Tolnaftate solution in polyethylene glycol 400 for 24 hours, the mean amount of Tolnaftate penetrating into the epidermis and dermis was 2.60 ± 0.28 μg/cm2 and 0.92 ± 0.12 μg/cm2, respectively. A validated reliable HPLC method could be recommended for biopharmaceutical evaluation of Tolnaftate preparations and studies of pharmacokinetics in human skin after in vitro penetration studiesBiochemijos katedraLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Assay of Tolnaftate in human skin samples after in vitro penetration studies using high performance liquid chromatography
    2020
    Co-Authors: Kežutytė Toma, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tolnaftate, an antifungal of thiocarbamate class, is used topically in 1% formulations. Its penetration into skin layers is a prerequisite for Tolnaftate action against dermatophytes. The aim of this work was to optimize and validate a simple, rapid, accurate and reproducible procedure for Tolnaftate assay in human skin samples and to apply this procedure for in vitro Tolnaftate penetration studies. High performance liquid chromatography (HPLC) method with UV detection was used to validate Tolnaftate assay for linearity, specificity, accuracy, precision, limit of quantitation, limit of detection, drug extraction recovery and stability in skin extracts. In vitro Tolnaftate penetration studies were carried out using flow-through diffusion cells, mounted with human skin. Epidermis and dermis, separated by heat-separation method, were extracted using ultrasonication in methanol. Linear range of the analytical procedure was within 0.6ñ100 μg/mL. The assay was specific, accurate (within-day and between-day recovery values were 98.2ñ104.2% and 98.7ñ101.4%, respectively) and precise (within-day and between-day imprecision was = 3.8%). Mean extraction recoveries of Tolnaftate from epidermis and dermis were satisfactory and reaching 90%. In vitro skin penetration studies revealed that after application of 1% (w/w) Tolnaftate solution in polyethylene glycol 400 for 24 hours, the mean amount of Tolnaftate penetrating into the epidermis and dermis was 2.60 ± 0.28 μg/cm2 and 0.92 ± 0.12 μg/cm2, respectively. A validated reliable HPLC method could be recommended for biopharmaceutical evaluation of Tolnaftate preparations and studies of pharmacokinetics in human skin after in vitro penetration studiesBiochemijos katedraGamtos mokslų fakultetasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Tolnaftate penetration into human skin ex vivo from solution and ointment formulations containing chemical penetration enchancers
    2015
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tyrinėtas į tirpalą ir tepalą įvestų oleino, linolo, lauro ir kapro riebiųjų rūgščių skatinantis poveikis tolnaftato skverbimuisi į žmogaus odą ex vivo. Riebiosios rūgštys priklauso lipofiliniams cheminiams skvarbos skatintojams, kurie gali sutrikdyti stratum corneum lipidų išsidėstymą ir paskatinti hidrofobinių vaistų, tokių kaip tolnaftatas, sverbimąsi. Tolnaftato 1% (m/m) tirpalas gautas ištirpinus jį polietilenglikolyje (PEG) 400, o 1% (m/m) tepalas - įvedus tiirpalą į baltojo vazelino, bevandenio lanolino ir PEG 1500 lydinį. Į abi vaisto formas pridėta po 10% (m/m) tyrinėtų riebiųjų rūgščių. [...]Oleic, linoleic, lauric and capric fatty acids were incorporated in 10% (w/w) into solution and ointment formulations and their enhancing effect on Tolnaftate penetration into human skin ex vivo was investigated. Fatty acids belong to the lipophilic chemical penetration enhancers (CPEs), which might alter stratum corneum lipid organization and increase the penetration of hydrophobic active drugs, such as Tolnaftate. Tolnaftate in 1% (w/w) was dissolved in polyethylene glycol (PEG) 400 or incorporated into an ointment, composed of white vaseline, anhydrous lanolin, PEG 400 and PEG 1500. 10% (w/w) of each fatty acid were added to both formulations. [...]Biochemijos katedraLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Hayam M Lotfy - One of the best experts on this subject based on the ideXlab platform.

  • coupling of liquid liquid extraction and mathematical filtration techniques for the separation and quantification of five components in semisolid dosage form with severely overlapped spectra
    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2020
    Co-Authors: Hayam M Lotfy, Nesma Mahmoud Fahmy
    Abstract:

    Abstract Quadriderm cream was a combination of four components; Clioquinol (CLIO), Betamethasone (BETA), Tolnaftate (TOL), Gentamicin (GEN) in addition to the preservative Chlorocresol (CC). Four components CLIO, TOL, BETA, and CC were extracted in methanol and determined by mathematic filtration spectrophotometric techniques. The partially overlapped spectrum of CLIO was determined by constant value, constant multiplication, and concentration value methods then eliminated via spectrum subtraction (SS) to get the resolved ternary mixture of TOL, BETA, and CC with severely overlapping spectra. TOL was determined by derivative ratio at zero crossing point of BETA using CC as a divisor. While, BETA could be determined using TOL as a divisor at zero crossing of CC. BETA and CC were obtained using novel (DD1FS) followed by SS. By applying these novel procedures, the DD1 spectrum of each component alone was recovered where Pmax-min was directly proportional to its concentration. Liquid-liquid extraction technique was used for the semisolid dosage form where GEN was extracted with a mixture of chloroform: water (50:50, v/v); and the induced fluorescence obtained by derivatization with o-phthalaldehyde was measured at 419 nm after excitation at 359 nm. Accuracy and precision testing of the developed methods showed good results. Specificity of the methods was ensured and was successfully applied for the analysis of pharmaceutical formulation of the five components in combination. ICH guidelines were used for validation of the proposed methods. Statistical data were calculated, and the results were satisfactory revealing no significant difference regarding accuracy and precision.

  • selective liquid chromatographic quantification of betamethasone valerate and clioquinol in presence of potential interferents
    International Journal of Pharmacy and Pharmaceutical Sciences, 2014
    Co-Authors: Hayam M Lotfy, Ezzat M Abdelmoety, Eman G Nouman
    Abstract:

    Objective : To develop and justify a validated simple and selective RP-HPLC method for simultaneous determination of betamethasone valerate (BETA), clioquinol (CLIO) together with their potential interferents including their proposed degradation products, the preservatives methyl paraben (MPB) and propyl paraben (PPB) as well as gentamycin and Tolnaftate. Methods : Degradation products of betamethasone and clioquinol were prepared then the technique was built using an efficient chromatographic separation on a Zorbax C 18 column (25 cm×4.6 mm, 5.0 μm) using water- methanol-acetonitrile- glacial acetic acid (394: 50: 550: 6, v/v/v/v) as mobile phase and the eluent was monitored at 275 nm. Results : The developed method was linear over the concentration ranges of 12-240 mg mL -1 , 30-3000 mg mL -1 , 7-140 mg mL -1 and 3.5-70 mg mL- 1 forBETA, CLIO, MPB and PPB, respectively, with high degree of accuracy and precision. Conclusion : The method was successfully applied for the analysis of BETA and CLIO in their pharmaceutical preparations and their combined formulation with gentamycin and Tolnaftate. Recoveries were quantitative, and the results obtained agreed with those obtained by official methods. Keywords : Betamethasone, Clioquinol, Degradation, HPLC, Stability, potential interferents.

Tahereh Shokohi - One of the best experts on this subject based on the ideXlab platform.

  • Molecular Identification and Antifungal Susceptibility of Yeasts and Molds Isolated from Patients with Otomycosis
    Mycopathologia, 2021
    Co-Authors: Keyvan Kiakojuri, Firoozeh Kermani, Javad Javidnia, Mohammad Taghi Hedayati, Iman Haghani, Tahereh Shokohi, Saeid Mahdavi Omran, Somayeh Roodgari, Mojtaba Taghizadeh Armaki, Hamid Badali
    Abstract:

    Fungal otitis externa, an infection of the external auditory canal caused by molds and yeasts, accounts for approximately 10–20% of ear canal infections accompanying high recurrence. The purpose of the current study was to assess the pattern of etiological agents of otomycosis and resistance profile as well as the rate of tympanic membrane perforation. A total of 1040 patients with symptoms of fungal otitis externa, in a period of two years, were investigated. The mycological tests revealed the presence of different fungi in 237 ears (22.8%). Fungal otitis was more related to filamentous fungi of the species Aspergillus flavus (54.43%), A. tubingensis (10.97%), and A. niger (8.86%), followed by yeasts, Candida orthopsilosis (7.59%), C. albicans (6.75%), and C. parapsilosis (5.06%). Tympanic membrane perforation rate was found to be 6.75% and was more common with otomycosis caused by A. flavus , A. tubingensis and C. albicans . In antifungal susceptibility tests, all tested drugs showed generally good activity against most isolates of molds and yeasts, while Tolnaftate, clotrimazole, nystatin, and terbinafine had lowest effects. We found that among Aspergillus isolates, one A. niger isolate was resistant to voriconazole, and one A. flavus isolate was resistant to amphotericin B. Furthermore, among Candida species, three isolates of C. orthopsilosis showed high MIC values to fluconazole, two C. albicans isolates were considered fluconazole resistant and one isolate of C. parapsilosis was resistant to caspofungin and 3 isolates were resistant to fluconazole. Regarding the existence of the cases with perforated tympanic membrane and emerging species causing fungal otitis in the current report, the importance of the early physical examination, precise molecular identification, and the antifungal susceptibility evaluation is highlighted.

  • In vitro activities of antifungal drugs against a large collection of Trichophyton tonsurans isolated from wrestlers.
    Mycoses, 2020
    Co-Authors: Firoozeh Kermani, Javad Javidnia, Mohammad Taghi Hedayati, Abastabar, Iman Haghani, Mojtaba Didehdar, Mahmoud Fami Zaghrami, Tahereh Shokohi
    Abstract:

    Background Trichophyton tonsurans is the most common agent causing tinea gladiatorum in wrestlers and limited data on susceptibility profiles of Trichophyton tonsurans is available. Objectives We aimed to assess the in vitro activity of the common antifungal drug against a large collection of T. tonsurans. Materials/methods The in vitro activities to eight common antifungal drugs (sertaconazole, itraconazole, clotrimazole, fluconazole, butenafine, Tolnaftate, terbinafine, and griseofulvin) against 128 clinical isolates of T. tonsurans strains, obtained from wrestlers with dermatophytosis, was performed according to CLSI M38-A2 broth microdilution document. Results The geometric mean minimum inhibitory concentration was the lowest for Tolnaftate (0.022 µg/ml), followed by itraconazole (0.026 µg/ml), terbinafine (0.033 µg/ml), butenafine (0.088 µg/ml), griseofulvin (0.566 µg/ml), sertaconazole (2.875 µg/ml), clotrimazole (3.419 µg/ml), and fluconazole (12.540 µg/ml). Conclusions Evaluation of antifungal susceptibility of dermatophytes showed that Tolnaftate and itraconazole were the most effective drugs against Trichophyton tonsurans and fluconazole had the least effect.

  • In vitro activity of new azoles luliconazole and lanoconazole compared with ten other antifungal drugs against clinical dermatophyte isolates
    Medical Mycology, 2016
    Co-Authors: Nesa Baghi, Iman Haghani, Mojtaba Didehdar, Tahereh Shokohi, Hamid Badali, Koichi Makimura, Ali Rezaei-matehkolaei, Maryam Abdollahi, Abastabar
    Abstract:

    In vitro susceptibilities of 100 clinical dermatophyte isolates belonging to five species from Iran toward lanoconazole and luliconazole were compared with ten other antifungal agents including econazole, itraconazole, miconazole, fluconazole, griseofulvin, butenafine, terbinafine, caspofungin, anidulafungin and Tolnaftate. MIC and MEC values were analyzed according to CLSI M38-A2 document. The isolates were previously identified to the species level using PCR-RFLP on ITS rDNA region. The range of luliconazole and lanoconazole minimum inhibitory concentrations (MICs) was 0.016-0.032 and 0.063-1 μg/ml, respectively for dermatophyte species. Luliconazole and lanoconazole revealed potent activity against all dermatophyte isolates. Anidulafungin, caspofungin, and luliconazole showed the best activity with the lowest geometric mean 0.01, 0.016, and 0.018 μg/ml, respectively, followed by Tolnaftate (0.06 μg/ml), terbinafine (0.07 μg/ml), itraconazole (0.183 μg/ml), butenafine (0.188 μg/ml), econazole (0.20 μg/ml), lanoconazole (0.24 μg/ml), griseofulvin (1.28 μg/ml), miconazole (2.34 μg/ml) and fluconazole (15.34 μg/ml). The current study demonstrated luliconazole and lanoconazole displayed excellent activity against all dermatophyte isolates, although the majority of dermatophyte isolates showed low susceptibility to griseofulvin and very low to miconazole, and fluconazole.

Maruška Audrius - One of the best experts on this subject based on the ideXlab platform.

  • Study of Tolnaftate release from fatty acids containing ointment and penetration into human skin ex vivo
    2020
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Rimdeika Rytis, Briedis Vitalis
    Abstract:

    Five fatty acids (oleic, linoleic, myristic, lauric and capric) were incorporated in 10% (w/w) into ointment formulation and their influence on lipophilic model drug Tolnaftate release in vitro and enhancing effect on Tolnaftate penetration into epidermis and dermis of human skin ex vivo were investigated. The prepared ointments were tested for homogeneity, pH and rheological properties. In vitro release studies and ex vivo skin penetration experiments were carried out using Hanson and Bronaugh-type flow-through diffusion cells, respectively. Tolnaftate cumulative amount liberated from semisolids was assayed using UV-Vis spectrophotometer. After in vitro skin penetration studies, appropriately extracted human skin layers were analyzed for Tolnaftate content using a validated HPLC method. Statistical analysis revealed that release rate of Tolnaftate from control ointment and ointments with fatty acids was not significantly different and only 7.34ñ8.98% of drug was liberated into an acceptor medium after 6 h. Tolnaftate amount penetrating into 1 cm2 of epidermis from ointments containing oleic, linoleic, myristic and lauric acids was significantly greater (p < 0.05) than from the control ointment. Penetration enhancing ratios for these fatty acids for Tolnaftate penetration into epidermis ranged from 1.48 to 1.75. In conclusion, fatty acids did not increase the liberation of Tolnaftate from ointment formulation, but demonstrated their enhancing effect on Tolnaftate penetration into human epidermis in vitro. Results from in vitro release experiments do not suit for prediction of the situation in the skin in vitro, if chemical penetration enhancers are incorporated into the ointment formulationBiochemijos katedraGamtos mokslų fakultetasLietuvos Sveikatos mokslų universiteto ligoninėLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Study of Tolnaftate release from fatty acids containing ointment and penetration into human skin ex vivo
    2020
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Rimdeika Rytis, Briedis Vitalis
    Abstract:

    Five fatty acids (oleic, linoleic, myristic, lauric and capric) were incorporated in 10% (w/w) into ointment formulation and their influence on lipophilic model drug Tolnaftate release in vitro and enhancing effect on Tolnaftate penetration into epidermis and dermis of human skin ex vivo were investigated. The prepared ointments were tested for homogeneity, pH and rheological properties. In vitro release studies and ex vivo skin penetration experiments were carried out using Hanson and Bronaugh-type flow-through diffusion cells, respectively. Tolnaftate cumulative amount liberated from semisolids was assayed using UV-Vis spectrophotometer. After in vitro skin penetration studies, appropriately extracted human skin layers were analyzed for Tolnaftate content using a validated HPLC method. Statistical analysis revealed that release rate of Tolnaftate from control ointment and ointments with fatty acids was not significantly different and only 7.34ñ8.98% of drug was liberated into an acceptor medium after 6 h. Tolnaftate amount penetrating into 1 cm2 of epidermis from ointments containing oleic, linoleic, myristic and lauric acids was significantly greater (p < 0.05) than from the control ointment. Penetration enhancing ratios for these fatty acids for Tolnaftate penetration into epidermis ranged from 1.48 to 1.75. In conclusion, fatty acids did not increase the liberation of Tolnaftate from ointment formulation, but demonstrated their enhancing effect on Tolnaftate penetration into human epidermis in vitro. Results from in vitro release experiments do not suit for prediction of the situation in the skin in vitro, if chemical penetration enhancers are incorporated into the ointment formulationBiochemijos katedraLietuvos Sveikatos mokslų universiteto ligoninėLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Assay of Tolnaftate in human skin samples after in vitro penetration studies using high performance liquid chromatography
    2020
    Co-Authors: Kežutytė Toma, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tolnaftate, an antifungal of thiocarbamate class, is used topically in 1% formulations. Its penetration into skin layers is a prerequisite for Tolnaftate action against dermatophytes. The aim of this work was to optimize and validate a simple, rapid, accurate and reproducible procedure for Tolnaftate assay in human skin samples and to apply this procedure for in vitro Tolnaftate penetration studies. High performance liquid chromatography (HPLC) method with UV detection was used to validate Tolnaftate assay for linearity, specificity, accuracy, precision, limit of quantitation, limit of detection, drug extraction recovery and stability in skin extracts. In vitro Tolnaftate penetration studies were carried out using flow-through diffusion cells, mounted with human skin. Epidermis and dermis, separated by heat-separation method, were extracted using ultrasonication in methanol. Linear range of the analytical procedure was within 0.6ñ100 μg/mL. The assay was specific, accurate (within-day and between-day recovery values were 98.2ñ104.2% and 98.7ñ101.4%, respectively) and precise (within-day and between-day imprecision was = 3.8%). Mean extraction recoveries of Tolnaftate from epidermis and dermis were satisfactory and reaching 90%. In vitro skin penetration studies revealed that after application of 1% (w/w) Tolnaftate solution in polyethylene glycol 400 for 24 hours, the mean amount of Tolnaftate penetrating into the epidermis and dermis was 2.60 ± 0.28 μg/cm2 and 0.92 ± 0.12 μg/cm2, respectively. A validated reliable HPLC method could be recommended for biopharmaceutical evaluation of Tolnaftate preparations and studies of pharmacokinetics in human skin after in vitro penetration studiesBiochemijos katedraLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Assay of Tolnaftate in human skin samples after in vitro penetration studies using high performance liquid chromatography
    2020
    Co-Authors: Kežutytė Toma, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tolnaftate, an antifungal of thiocarbamate class, is used topically in 1% formulations. Its penetration into skin layers is a prerequisite for Tolnaftate action against dermatophytes. The aim of this work was to optimize and validate a simple, rapid, accurate and reproducible procedure for Tolnaftate assay in human skin samples and to apply this procedure for in vitro Tolnaftate penetration studies. High performance liquid chromatography (HPLC) method with UV detection was used to validate Tolnaftate assay for linearity, specificity, accuracy, precision, limit of quantitation, limit of detection, drug extraction recovery and stability in skin extracts. In vitro Tolnaftate penetration studies were carried out using flow-through diffusion cells, mounted with human skin. Epidermis and dermis, separated by heat-separation method, were extracted using ultrasonication in methanol. Linear range of the analytical procedure was within 0.6ñ100 μg/mL. The assay was specific, accurate (within-day and between-day recovery values were 98.2ñ104.2% and 98.7ñ101.4%, respectively) and precise (within-day and between-day imprecision was = 3.8%). Mean extraction recoveries of Tolnaftate from epidermis and dermis were satisfactory and reaching 90%. In vitro skin penetration studies revealed that after application of 1% (w/w) Tolnaftate solution in polyethylene glycol 400 for 24 hours, the mean amount of Tolnaftate penetrating into the epidermis and dermis was 2.60 ± 0.28 μg/cm2 and 0.92 ± 0.12 μg/cm2, respectively. A validated reliable HPLC method could be recommended for biopharmaceutical evaluation of Tolnaftate preparations and studies of pharmacokinetics in human skin after in vitro penetration studiesBiochemijos katedraGamtos mokslų fakultetasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

  • Tolnaftate penetration into human skin ex vivo from solution and ointment formulations containing chemical penetration enchancers
    2015
    Co-Authors: Kežutytė Toma, Drevinskas Tomas, Maruška Audrius, Kornyšova Olga, Briedis Vitalis
    Abstract:

    Tyrinėtas į tirpalą ir tepalą įvestų oleino, linolo, lauro ir kapro riebiųjų rūgščių skatinantis poveikis tolnaftato skverbimuisi į žmogaus odą ex vivo. Riebiosios rūgštys priklauso lipofiliniams cheminiams skvarbos skatintojams, kurie gali sutrikdyti stratum corneum lipidų išsidėstymą ir paskatinti hidrofobinių vaistų, tokių kaip tolnaftatas, sverbimąsi. Tolnaftato 1% (m/m) tirpalas gautas ištirpinus jį polietilenglikolyje (PEG) 400, o 1% (m/m) tepalas - įvedus tiirpalą į baltojo vazelino, bevandenio lanolino ir PEG 1500 lydinį. Į abi vaisto formas pridėta po 10% (m/m) tyrinėtų riebiųjų rūgščių. [...]Oleic, linoleic, lauric and capric fatty acids were incorporated in 10% (w/w) into solution and ointment formulations and their enhancing effect on Tolnaftate penetration into human skin ex vivo was investigated. Fatty acids belong to the lipophilic chemical penetration enhancers (CPEs), which might alter stratum corneum lipid organization and increase the penetration of hydrophobic active drugs, such as Tolnaftate. Tolnaftate in 1% (w/w) was dissolved in polyethylene glycol (PEG) 400 or incorporated into an ointment, composed of white vaseline, anhydrous lanolin, PEG 400 and PEG 1500. 10% (w/w) of each fatty acid were added to both formulations. [...]Biochemijos katedraLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta