The Experts below are selected from a list of 11937 Experts worldwide ranked by ideXlab platform

Cori Cauglin - One of the best experts on this subject based on the ideXlab platform.

  • a novel sensitive high performance liquid chromatography electrochemical procedure for measuring formaldehyde produced from oxidative deamination of methylamine and in biological samples
    Analytical Biochemistry, 2003
    Co-Authors: Cori Cauglin, Krista L Wempe, Diana Gubisnehaberle
    Abstract:

    Formaldehyde is a well-known environmental Toxic Hazard. It is also a product of oxidative deamination of methylamine catalyzed by semicarbazide-sensitive amine oxidase (SSAO). Increased SSAO-mediated deamination has been implicated in some pathophysiological conditions, such as diabetic complications. The measurement of formaldehyde in the enzymatic reactions and in vivo production using conventional methods was not straightforward due to limitations of selectivity and sensitivity. A novel high-performance liquid chromatography (HPLC)/electrochemical procedure for the measurement of formaldehyde has been developed. The measurement is based on the formation of adducts between formaldehyde and dopamine. These adducts can be selectively purified and concentrated using a batch method of alumina absorption, separated by HPLC, and electrochemically quantified. The method is highly selective and substantially more sensitive, i.e., detection of picomole levels of formaldehyde, than the conventional methods. The procedure not only facilitates the assessment of SSAO activity in vitro but also is useful for assessing formaldehyde in tissues and biological fluids.

  • A novel sensitive high-performance liquid chromatography/electrochemical procedure for measuring formaldehyde produced from oxidative deamination of methylamine and in biological samples
    Analytical Biochemistry, 2003
    Co-Authors: Cori Cauglin, Krista L Wempe, Diana Gubisne-haberle
    Abstract:

    Formaldehyde is a well-known environmental Toxic Hazard. It is also a product of oxidative deamination of methylamine catalyzed by semicarbazide-sensitive amine oxidase (SSAO). Increased SSAO-mediated deamination has been implicated in some pathophysiological conditions, such as diabetic complications. The measurement of formaldehyde in the enzymatic reactions and in vivo production using conventional methods was not straightforward due to limitations of selectivity and sensitivity. A novel high-performance liquid chromatography (HPLC)/electrochemical procedure for the measurement of formaldehyde has been developed. The measurement is based on the formation of adducts between formaldehyde and dopamine. These adducts can be selectively purified and concentrated using a batch method of alumina absorption, separated by HPLC, and electrochemically quantified. The method is highly selective and substantially more sensitive, i.e., detection of picomole levels of formaldehyde, than the conventional methods. The procedure not only facilitates the assessment of SSAO activity in vitro but also is useful for assessing formaldehyde in tissues and biological fluids.

Diana Gubisne-haberle - One of the best experts on this subject based on the ideXlab platform.

  • A novel sensitive high-performance liquid chromatography/electrochemical procedure for measuring formaldehyde produced from oxidative deamination of methylamine and in biological samples
    Analytical Biochemistry, 2003
    Co-Authors: Cori Cauglin, Krista L Wempe, Diana Gubisne-haberle
    Abstract:

    Formaldehyde is a well-known environmental Toxic Hazard. It is also a product of oxidative deamination of methylamine catalyzed by semicarbazide-sensitive amine oxidase (SSAO). Increased SSAO-mediated deamination has been implicated in some pathophysiological conditions, such as diabetic complications. The measurement of formaldehyde in the enzymatic reactions and in vivo production using conventional methods was not straightforward due to limitations of selectivity and sensitivity. A novel high-performance liquid chromatography (HPLC)/electrochemical procedure for the measurement of formaldehyde has been developed. The measurement is based on the formation of adducts between formaldehyde and dopamine. These adducts can be selectively purified and concentrated using a batch method of alumina absorption, separated by HPLC, and electrochemically quantified. The method is highly selective and substantially more sensitive, i.e., detection of picomole levels of formaldehyde, than the conventional methods. The procedure not only facilitates the assessment of SSAO activity in vitro but also is useful for assessing formaldehyde in tissues and biological fluids.

Diana Gubisnehaberle - One of the best experts on this subject based on the ideXlab platform.

  • a novel sensitive high performance liquid chromatography electrochemical procedure for measuring formaldehyde produced from oxidative deamination of methylamine and in biological samples
    Analytical Biochemistry, 2003
    Co-Authors: Cori Cauglin, Krista L Wempe, Diana Gubisnehaberle
    Abstract:

    Formaldehyde is a well-known environmental Toxic Hazard. It is also a product of oxidative deamination of methylamine catalyzed by semicarbazide-sensitive amine oxidase (SSAO). Increased SSAO-mediated deamination has been implicated in some pathophysiological conditions, such as diabetic complications. The measurement of formaldehyde in the enzymatic reactions and in vivo production using conventional methods was not straightforward due to limitations of selectivity and sensitivity. A novel high-performance liquid chromatography (HPLC)/electrochemical procedure for the measurement of formaldehyde has been developed. The measurement is based on the formation of adducts between formaldehyde and dopamine. These adducts can be selectively purified and concentrated using a batch method of alumina absorption, separated by HPLC, and electrochemically quantified. The method is highly selective and substantially more sensitive, i.e., detection of picomole levels of formaldehyde, than the conventional methods. The procedure not only facilitates the assessment of SSAO activity in vitro but also is useful for assessing formaldehyde in tissues and biological fluids.

Krista L Wempe - One of the best experts on this subject based on the ideXlab platform.

  • a novel sensitive high performance liquid chromatography electrochemical procedure for measuring formaldehyde produced from oxidative deamination of methylamine and in biological samples
    Analytical Biochemistry, 2003
    Co-Authors: Cori Cauglin, Krista L Wempe, Diana Gubisnehaberle
    Abstract:

    Formaldehyde is a well-known environmental Toxic Hazard. It is also a product of oxidative deamination of methylamine catalyzed by semicarbazide-sensitive amine oxidase (SSAO). Increased SSAO-mediated deamination has been implicated in some pathophysiological conditions, such as diabetic complications. The measurement of formaldehyde in the enzymatic reactions and in vivo production using conventional methods was not straightforward due to limitations of selectivity and sensitivity. A novel high-performance liquid chromatography (HPLC)/electrochemical procedure for the measurement of formaldehyde has been developed. The measurement is based on the formation of adducts between formaldehyde and dopamine. These adducts can be selectively purified and concentrated using a batch method of alumina absorption, separated by HPLC, and electrochemically quantified. The method is highly selective and substantially more sensitive, i.e., detection of picomole levels of formaldehyde, than the conventional methods. The procedure not only facilitates the assessment of SSAO activity in vitro but also is useful for assessing formaldehyde in tissues and biological fluids.

  • A novel sensitive high-performance liquid chromatography/electrochemical procedure for measuring formaldehyde produced from oxidative deamination of methylamine and in biological samples
    Analytical Biochemistry, 2003
    Co-Authors: Cori Cauglin, Krista L Wempe, Diana Gubisne-haberle
    Abstract:

    Formaldehyde is a well-known environmental Toxic Hazard. It is also a product of oxidative deamination of methylamine catalyzed by semicarbazide-sensitive amine oxidase (SSAO). Increased SSAO-mediated deamination has been implicated in some pathophysiological conditions, such as diabetic complications. The measurement of formaldehyde in the enzymatic reactions and in vivo production using conventional methods was not straightforward due to limitations of selectivity and sensitivity. A novel high-performance liquid chromatography (HPLC)/electrochemical procedure for the measurement of formaldehyde has been developed. The measurement is based on the formation of adducts between formaldehyde and dopamine. These adducts can be selectively purified and concentrated using a batch method of alumina absorption, separated by HPLC, and electrochemically quantified. The method is highly selective and substantially more sensitive, i.e., detection of picomole levels of formaldehyde, than the conventional methods. The procedure not only facilitates the assessment of SSAO activity in vitro but also is useful for assessing formaldehyde in tissues and biological fluids.

Mats Westin - One of the best experts on this subject based on the ideXlab platform.

  • Toxic Hazard of leachates from furfurylated wood comparison between two different aquatic organisms
    Environmental Toxicology and Chemistry, 2010
    Co-Authors: Annica Pilgård, Liesbeth De Vetter, Joris Van Acker, Mats Westin
    Abstract:

    Environmental concern regarding the use of Toxic preservatives such as chromated copper arsenate (CCA) has been put forward. In the European Union, United States, and Japan, CCA has been phased out for residential and water-contact applications. EcoToxicological studies of wood treated with conventional preservatives were carried out in the late 1990s, and it was concluded that the main impact is to water and aquatic organisms. Today, alternatives to conventional preservation methods, marketed as “environmentally friendly” or “nonToxic,” are emerging. Examples of such alternatives are modified wood, e.g., thermally modified, furfurylated, and acetylated wood. To date, not enough Hazard characterization has been performed. In the present study, the Microtox® assay with the marine bacterium Vibrio fischeri and the Daphtox® procedure with the crustacean Daphnia magna were used as screening methods in an effect assessment. Both organisms were exposed to water leachates from furfurylated wood using two different leaching procedures. The results indicate that Microtox is more sensitive to the Toxic components from furfurylated wood than Daphtox. Furthermore, the Toxicity of treated Pinus radiata was higher than that of treated Pinus sylvestris. The Toxicity did not diminish over the test period, as is the case for preservative-treated wood. The present study found that treatment conditions can influence the Toxicity considerably, so Toxicity studies should be included in the development of new treatment process. The present study also shows that using an intermediate vacuum-drying step, leading to a more efficient curing/polymerization, results in slightly less hydrophobic oligomers in the product, such that the leachates become less Toxic to bacteria. Environ. Toxicol. Chem. 2010;29:1067–1071. © 2010 SETAC

  • Toxic Hazard and chemical analysis of leachates from furfurylated wood
    Environmental toxicology and chemistry, 2010
    Co-Authors: Annica Pilgård, Andreas Treu, Albert N. T. Van Zeeland, Richard Johannes Antonius Gosselink, Mats Westin
    Abstract:

    The furfurylation process is an extensively investigated wood modification process. Furfuryl alcohol molecules penetrate into the wood cell wall and polymerize in situ. This results in a permanent swelling of the wood cell walls. It is unclear whether or not chemical bonds exist between the furfuryl alcohol polymer and the wood. In the present study, five different wood species were used, both hardwoods and softwoods. They were treated with three different furfurylation procedures and leached according to three different leaching methods. The present study shows that, in general, the leachates from furfurylated wood have low Toxicity. It also shows that the choice of leaching method is decisive for the outcome of the Toxicity results. Earlier studies have shown that leachates from wood treated with furfuryl alcohol prepolymers have higher Toxicity to Vibrio fischeri than leachates from wood treated with furfuryl alcohol monomers. This is probably attributable to differences in leaching of chemical compounds. The present study shows that this difference in the Toxicity most likely cannot be attributed to maleic acid, furan, furfural, furfuryl alcohol, or 2-furoic acid. However, the difference might be caused by the two substances 5-hydroxymethylfurfural and 2,5-furandimethanol. The present study found no difference in the amount of leached furfuryl alcohol between leachates from furfurylated softwood and furfurylated hardwood species. Earlier studies have indicated differences in grafting of furfuryl alcohol to lignin. However, nothing was found in the present study that could support this. The leachates of furfurylated wood still need to be investigated further to identify the chemical differences between wood furfurylated with furfuryl alcohol monomers and furfuryl alcohol prepolymers. Environ. Toxicol. Chem. 2010;29:1918–1924. © 2010 SETAC