The Experts below are selected from a list of 59448 Experts worldwide ranked by ideXlab platform
Geoffrey A Codd - One of the best experts on this subject based on the ideXlab platform.
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immuno crossreactivity and Toxicity Assessment of conjugation products of the cyanobacterial toxin microcystin lr
Fems Microbiology Letters, 2000Co-Authors: James S Metcalf, Kenneth A Beattie, Geoffrey A CoddAbstract:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96–112%) to that of microcystin-LR, when assayed at a concentration of 1 μg l−1. Toxicity Assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3–9-fold lower Toxicity than microcystin-LR.
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immuno crossreactivity and Toxicity Assessment of conjugation products of the cyanobacterial toxin microcystin lr
Fems Microbiology Letters, 2000Co-Authors: James S Metcalf, Kenneth A Beattie, Geoffrey A CoddAbstract:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96–112%) to that of microcystin-LR, when assayed at a concentration of 1 μg l−1. Toxicity Assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3–9-fold lower Toxicity than microcystin-LR.
James S Metcalf - One of the best experts on this subject based on the ideXlab platform.
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immuno crossreactivity and Toxicity Assessment of conjugation products of the cyanobacterial toxin microcystin lr
Fems Microbiology Letters, 2000Co-Authors: James S Metcalf, Kenneth A Beattie, Geoffrey A CoddAbstract:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96–112%) to that of microcystin-LR, when assayed at a concentration of 1 μg l−1. Toxicity Assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3–9-fold lower Toxicity than microcystin-LR.
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immuno crossreactivity and Toxicity Assessment of conjugation products of the cyanobacterial toxin microcystin lr
Fems Microbiology Letters, 2000Co-Authors: James S Metcalf, Kenneth A Beattie, Geoffrey A CoddAbstract:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96–112%) to that of microcystin-LR, when assayed at a concentration of 1 μg l−1. Toxicity Assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3–9-fold lower Toxicity than microcystin-LR.
Colin R Janssen - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the mayfly ephoron virgo for european sediment Toxicity Assessment
Journal of Soils and Sediments, 2012Co-Authors: Lien T H Nguyen, Michiel B Vandegehuchte, H G Van Der Geest, Colin R JanssenAbstract:Purpose The mayfly Ephoron virgo plays a key role in the ecological recovery in large European rivers. The larvae spend their life in the sediment and are therefore exposed to contaminants in various ways. In this study, the use of E. virgo as a test species for whole sediment bioassays was evaluated. The sensitivity was compared with Hexagenia limbata (a North American mayfly recommended for standard sediment testing). Lead (Pb) was used as a model contaminant.
Kenneth A Beattie - One of the best experts on this subject based on the ideXlab platform.
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immuno crossreactivity and Toxicity Assessment of conjugation products of the cyanobacterial toxin microcystin lr
Fems Microbiology Letters, 2000Co-Authors: James S Metcalf, Kenneth A Beattie, Geoffrey A CoddAbstract:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96–112%) to that of microcystin-LR, when assayed at a concentration of 1 μg l−1. Toxicity Assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3–9-fold lower Toxicity than microcystin-LR.
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immuno crossreactivity and Toxicity Assessment of conjugation products of the cyanobacterial toxin microcystin lr
Fems Microbiology Letters, 2000Co-Authors: James S Metcalf, Kenneth A Beattie, Geoffrey A CoddAbstract:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96–112%) to that of microcystin-LR, when assayed at a concentration of 1 μg l−1. Toxicity Assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3–9-fold lower Toxicity than microcystin-LR.
Lien T H Nguyen - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the mayfly ephoron virgo for european sediment Toxicity Assessment
Journal of Soils and Sediments, 2012Co-Authors: Lien T H Nguyen, Michiel B Vandegehuchte, H G Van Der Geest, Colin R JanssenAbstract:Purpose The mayfly Ephoron virgo plays a key role in the ecological recovery in large European rivers. The larvae spend their life in the sediment and are therefore exposed to contaminants in various ways. In this study, the use of E. virgo as a test species for whole sediment bioassays was evaluated. The sensitivity was compared with Hexagenia limbata (a North American mayfly recommended for standard sediment testing). Lead (Pb) was used as a model contaminant.