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S Haryati - One of the best experts on this subject based on the ideXlab platform.

  • Cloning and molecular analysis of Toxoplasma gondii Surface Antigen 2 (SAG2) gene cloned from Toxoplasma gondii DNA isolated from Javanese acute toxoplasmosis
    IOP Conference Series: Materials Science and Engineering, 2018
    Co-Authors: W Monica, A A Prasetyo, S Haryati
    Abstract:

    The Toxoplasma gondii Surface Antigens 2 (SAG2) is an immunogenic antigen covering the Toxoplasma gondii and one of the main proteins expressed on the surface of the parasite tachyzoites. However, data of the Toxoplasma gondii SAG2 from Indonesian isolate is limited. To study the Toxoplasma gondii SAG2 from Indonesian isolate the gene for SAG2 was amplified from genomic DNA of Toxoplasma gondii isolated from a Javanese acute toxoplasmosis blood samples patient. The clone was sequencing, and the results were subjected to bioinformatics analysis. Physicochemical analysis revealed the 190 aa of SAG2 had 19.47 kDa of weight. The isoelectric point and aliphatic index were 8.61 and 73.105, respectively. The N-terminal methionine half-life in Escherichia coli was more than 10 hours. The antigenicity, secondary structure, and identification of the HLA binding motifs also had been discussed. These findings would contribute information about Toxoplasma gondii SAG2 from Indonesian isolate.

  • Molecular analysis of Toxoplasma gondii Surface Antigen 1 (SAG1) gene cloned from Toxoplasma gondii DNA isolated from Javanese acute toxoplasmosis
    Journal of Physics: Conference Series, 2018
    Co-Authors: S Haryati, A A Prasetyo, Yulia Sari, Ruben Dharmawan
    Abstract:

    Toxoplasma gondii Surface Antigen 1 (SAG1) is often used as a diagnostic tool due to its immunodominant-specific as antigen. However, data of the Toxoplasma gondii SAG1 protein from Indonesian isolate is limited. To study the protein, genomic DNA was isolated from a Javanese acute toxoplasmosis blood samples patient. A complete coding sequence of Toxoplasma gondii SAG1 was cloned and inserted into an Escherichia coli expression plasmid and sequenced. The sequencing results were subjected to bioinformatics analysis. The Toxoplasma gondii SAG1 complete coding sequences were successfully cloned. Physicochemical analysis revealed the 336 aa of SAG1 had 34.7 kDa of weight. The isoelectric point and aliphatic index were 8.4 and 78.4, respectively. The N-terminal methionine half-life in Escherichia coli was more than 10 hours. The antigenicity, secondary structure, and identification of the HLA binding motifs also had been discussed. The results of this study would contribute information about Toxoplasma gondii SAG1 and benefits for further works willing to develop diagnostic and therapeutic strategies against the parasite.

Wei-xia Zhong - One of the best experts on this subject based on the ideXlab platform.

  • Progress on surface antigens of Toxoplasma gondii
    2009
    Co-Authors: Wei-xia Zhong
    Abstract:

    Toxoplasma gondii is all obligate intracellular parasite that infects a wide range of hosts,in-cluding humans and domesticated animals throughout the wodd.The investigation of the antigens of Toxoplasma gondii is of great significance in developing diagnostic reagents,vaccines and studying on the relationship be-tween Toxoplasma gondii and its hosts.This review focused on the recent progress on the antigens of Toxoplas-ma gondii. Key words: Toxoplasma gondii; Surface antigen; lmmunogenieity

Ruben Dharmawan - One of the best experts on this subject based on the ideXlab platform.

  • Molecular analysis of Toxoplasma gondii Surface Antigen 1 (SAG1) gene cloned from Toxoplasma gondii DNA isolated from Javanese acute toxoplasmosis
    Journal of Physics: Conference Series, 2018
    Co-Authors: S Haryati, A A Prasetyo, Yulia Sari, Ruben Dharmawan
    Abstract:

    Toxoplasma gondii Surface Antigen 1 (SAG1) is often used as a diagnostic tool due to its immunodominant-specific as antigen. However, data of the Toxoplasma gondii SAG1 protein from Indonesian isolate is limited. To study the protein, genomic DNA was isolated from a Javanese acute toxoplasmosis blood samples patient. A complete coding sequence of Toxoplasma gondii SAG1 was cloned and inserted into an Escherichia coli expression plasmid and sequenced. The sequencing results were subjected to bioinformatics analysis. The Toxoplasma gondii SAG1 complete coding sequences were successfully cloned. Physicochemical analysis revealed the 336 aa of SAG1 had 34.7 kDa of weight. The isoelectric point and aliphatic index were 8.4 and 78.4, respectively. The N-terminal methionine half-life in Escherichia coli was more than 10 hours. The antigenicity, secondary structure, and identification of the HLA binding motifs also had been discussed. The results of this study would contribute information about Toxoplasma gondii SAG1 and benefits for further works willing to develop diagnostic and therapeutic strategies against the parasite.

Gu Jun-cha - One of the best experts on this subject based on the ideXlab platform.

Małgorzata Paul - One of the best experts on this subject based on the ideXlab platform.

  • Toxoplasma gondii-specific IgG avidity testing in pregnant women
    Clinical Microbiology and Infection, 2020
    Co-Authors: Cécile Garnaud, Isabelle Villena, Hélène Fricker-hidalgo, Birgitta Evengård, Míriam J Álvarez-martinez, Eskild Petersen, Laetitia Maria Kortbeek, Florence Robert-gangneux, Carmen Costache, Małgorzata Paul
    Abstract:

    Background - The parasite Toxoplasma gondii can cause congenital toxoplasmosis following primary infection in a pregnant woman. It is therefore important to distinguish between recent and past infection when both T. gondii-specific IgM and IgG are detected in a single serum in pregnant women. Toxoplasma gondii-specific IgG avidity testing is an essential tool to help to date the infection. However, interpretation of its results can be complex. Objectives - To review the benefits and limitations of T. gondii-specific avidity testing in pregnant women, to help practitioners to interpret the results and adapt the patient management. Sources - PubMed search with the keywords avidity, toxoplasmosis and Toxoplasma gondii for articles published from 1989 to 2019. Content - Toxoplasma gondii-specific IgG avidity testing remains a key tool for dating a T. gondii infection in immunocompetent pregnant women. Several commercial assays are available and display comparable performances. A high avidity result obtained on a first-trimester serum sample is indicative of a past infection, which occurred before pregnancy. To date, a low avidity result must still be considered as non-informative to date the infection, although some authors suggest that very low avidity results are highly suggestive of recent infections depending on the assay. Interpretation of low or grey zone avidity results on a first-trimester serum sample, as well as any avidity result on a second-trimester or third-trimester serum sample, is more complex and requires recourse to expert toxoplasmosis laboratories. Implications - Although used for about 30 years, T. gondii-specific avidity testing has scarcely evolved. The same difficulties in interpretation have persisted over the years. Some authors have proposed additional thresholds to exclude an infection of