The Experts below are selected from a list of 1437 Experts worldwide ranked by ideXlab platform

Zhang Luo - One of the best experts on this subject based on the ideXlab platform.

  • effects of focused ultrasound wave on Mucosa of rabbit Trachea
    Chinese Archives of Otolaryngology-head and Neck Surgery, 2009
    Co-Authors: Zhang Luo
    Abstract:

    OBJECTIVE To study the effect of focused ultrasound wave on lower respiratory and Trachea Mucosa of rabbit.METHODS Focused ultrasound scan was performed on rabbit Trachea in vitro.Pathological changes of Tracheal Mucosa were investigated.Part of Trachea Mucosa was seeded on collagen-coated cover slips for primary culture.Phase-contrast microscopy was used to detect the cilia activity.The phase-contrast images were captured with a high-speed digital camera which was a progressive scan charged-coupled device(CCD)that provided images at 240 frames per second(fps)and then to detect the gray-intensity variation in the region of interest(ROI).RESULTS Light microscopic examination showed that the epithelia of Tracheal Mucosa was intact and there was edema and diffused distribution of spot coagulated necroses in subMucosa.Degeneration of vascular endothelial cells and glandular cells necroses were found in the coagulated necrosis region.There wasn't significant difference of ciliary beat frequency between the rabbit Trachea treated with focused ultrasound and the control.CONCLUSION Focused ultrasound wave with therapy intensity could ablate subMucosa including blood vessels glands and respiratory mucous tissues.However there were no damage were found to the epithelial layer and cilia.ty could ablate subepithelia layer tissue including blood vessels glands and respiration mucous tissues.However there were no damage to the epithelial layer and cilia.

Yu Jing - One of the best experts on this subject based on the ideXlab platform.

  • effects of topical anesthesia of Trachea Mucosa with lidocaine before general anesthesia on analepsia quality
    Practical Clinical Medicine, 2012
    Co-Authors: Yu Jing
    Abstract:

    Objective To investigate the effects of topical anesthesia of Trachea Mucosa with lidocaine before general anesthesia on analepsia quality in patients undergoing laparoscopic cholecystectomy.Methods Forty ASAⅠ-Ⅱ patients(aged 35-55 years) undergoing laparoscopic cholecystectomy were randomly assigned to receive either 0.9% NaCl(control group) or 4 mL 2% lidocaine(experiment group) for topical anesthesia of lingual root,throat and Trachea Mucosa.Systolic blood pressure(SBP),diastolic blood pressure(DBP),heart rate(HR),visual analogue scale(VAS) scores,agitation degree and residence time in post-anesthctic ICU(PACU) were recorded in analepsia period.Results Compared with control group,SBP,DBP,HR and VAS scores significantly decreased in lidocaine group in analepsia period(P0.05).Conclusion Topical anesthesia of Trachea Mucosa with lidocaine before general anesthesia can improve the analepsia quality in patients undergoing laparoscopic cholecystectomy.

Nauwynck Hans - One of the best experts on this subject based on the ideXlab platform.

  • Us3 and Us9 proteins contribute to the stromal invasion of bovine herpesvirus 1 in the respiratory Mucosa
    'Microbiology Society', 2017
    Co-Authors: Zhao Jing, Poelaert Katrien, Steukers Lennert, Favoreel Herman, Li Yewei, Chowdhury Shafiqul, Van Drunen Littel-van Den Hurk Sylvia, Caij Brigitte, Nauwynck Hans
    Abstract:

    Bovine herpesvirus 1 (BHV-1) infection may lead to conjunctivitis, upper respiratory tract problems, pneumonia, genital disorders and abortion. BHV-1 is able to spread quickly in a plaque-wise manner and invade by breaching the basement membrane (BM) barrier in the respiratory Mucosa. BHV-1 Us3, a serine/threonine kinase, induces a dramatic cytoskeletal reorganization and BHV-1 Us9, a tail-anchored membrane protein, is required for axonal transport of viruses in neurons. In this study, we investigated the role of Us3 and Us9 during BHV-1 infection in the respiratory Mucosa. First, we constructed and characterized BHV-1 Us3 null, Us9 null and revertant viruses. Then, we analysed the viral replication and plaque size (latitude) in Madin-Darby bovine kidney (MDBK) cells and the respiratory Mucosa as well as viral penetration depth underneath the BM of the respiratory Mucosa when inoculated with these recombinant viruses. Knockout of Us3 resulted in a 1 log(10) reduction in viral titre and plaque size (latitude) in MDBK cells and the Trachea Mucosa. There were no defects in the cell-to-cell spread observed for BHV-1 Us9 null virus. Both BHV-1 Us3 null and Us9 null viruses showed a significant reduction of plaque penetration underneath the BM; however, penetration was not completely inhibited. In conclusion, the current findings demonstrated that Us3 and Us9 play an important role in the invasion of BHV-1 through the BM of the respiratory Mucosa, which shows the way forward for research-based attenuation of viruses in order to make safer and better-performing vaccines

Zhao Jing - One of the best experts on this subject based on the ideXlab platform.

  • Us3 and Us9 proteins contribute to the stromal invasion of bovine herpesvirus 1 in the respiratory Mucosa
    'Microbiology Society', 2017
    Co-Authors: Zhao Jing, Poelaert Katrien, Steukers Lennert, Favoreel Herman, Li Yewei, Chowdhury Shafiqul, Van Drunen Littel-van Den Hurk Sylvia, Caij Brigitte, Nauwynck Hans
    Abstract:

    Bovine herpesvirus 1 (BHV-1) infection may lead to conjunctivitis, upper respiratory tract problems, pneumonia, genital disorders and abortion. BHV-1 is able to spread quickly in a plaque-wise manner and invade by breaching the basement membrane (BM) barrier in the respiratory Mucosa. BHV-1 Us3, a serine/threonine kinase, induces a dramatic cytoskeletal reorganization and BHV-1 Us9, a tail-anchored membrane protein, is required for axonal transport of viruses in neurons. In this study, we investigated the role of Us3 and Us9 during BHV-1 infection in the respiratory Mucosa. First, we constructed and characterized BHV-1 Us3 null, Us9 null and revertant viruses. Then, we analysed the viral replication and plaque size (latitude) in Madin-Darby bovine kidney (MDBK) cells and the respiratory Mucosa as well as viral penetration depth underneath the BM of the respiratory Mucosa when inoculated with these recombinant viruses. Knockout of Us3 resulted in a 1 log(10) reduction in viral titre and plaque size (latitude) in MDBK cells and the Trachea Mucosa. There were no defects in the cell-to-cell spread observed for BHV-1 Us9 null virus. Both BHV-1 Us3 null and Us9 null viruses showed a significant reduction of plaque penetration underneath the BM; however, penetration was not completely inhibited. In conclusion, the current findings demonstrated that Us3 and Us9 play an important role in the invasion of BHV-1 through the BM of the respiratory Mucosa, which shows the way forward for research-based attenuation of viruses in order to make safer and better-performing vaccines

M N Kondrashova - One of the best experts on this subject based on the ideXlab platform.

  • the effect of inhaled air ions generated by technical ionizers and a bioionizer on rat Trachea Mucosa and the phagocytic activity of blood cells
    IEEE Transactions on Plasma Science, 2006
    Co-Authors: T V Sirota, V I Novoselov, V G Safronova, V A Yanin, V D Tsvetkov, S E Amelina, A L Lushnikova, V N Maltseva, V P Tikhonov, M N Kondrashova
    Abstract:

    It was shown for the first time that inhaling negative air ions (NAI) leads to changes in the functional state of the rat Trachea Mucosa, which in turn affects the phagocytic activity of blood cells. The effect depends on the concentration of the NAI, the duration of their action, and the sources of the NAI generation. For air ionization, technical ionizers and a bioionizer (a plant electrified through soil) developed by the Diod Company, Moscow, Russia were used. It was shown that the NAI at high doses (600 000 ion/cm3), generated by an Elion 131M device, induce a damage to the rat Trachea Mucosa, an inhibition of the activity of its antioxidant enzymes, and a decrease in the phagocytic activity of whole blood cells. Low doses of the NAI from the same source (25 000-50 000 ion/cm3) and high doses of the NAI from devices Elion 132S and 132R (320 000-500 000 ion/cm3), and a bioionizer (500 000 ion/cm3) produced a milder effect, activating the secretion of goblet cells of the Trachea Mucosa without its damage. A possible mechanism of the effect of inhaled NAI as exogenous reactive oxygen species (ROS) on internal processes in the organism is discussed. The involvement of the ROS in the action of ionized air found in the study is probably the general property of different electrical influences on biological objects. Therefore, the result of the investigation may be of interest for a wide circle of specialists dealing with ElectroMed influences