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Rita Rizky Rahmawati - One of the best experts on this subject based on the ideXlab platform.
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identification of javan langur Trachypithecus auratus in javan langur center jlc coban talun batu based on cytochrome b sequence
KnE Life Sciences, 2015Co-Authors: Rita Rizky Rahmawati, Emy Kusumawati, Lulus Putri Aninda, Miftahul Mushlih, Abdul Gofur, Dwi ListyoriniAbstract:Javan Langur (Trachypithecus auratus) is one of endemic primates in Indonesia and classified into vulnerable level. Based on its morphological characteristic and geographic area, Javan Langur divided into 2 subspecies, those are Trachypithecus auratus auratus and Trachypithecus auratus mauritius. The identification of its morphological characteristic and genetic in species or subspecies level is important before releasing because it is correlated with inbreeding grouping and releasing place. The aim of this study is to identify the Javan Langur (Trachypithecus auratus) in Javan Langur Center (JLC) Coban Talun-Batu based on D-Loop sequence. The blood sample used in this study was taken from two adult female Javan Langur named Ijem and Embun, that have different morphological characteristics. Both (primates) are suggested to be T.a.mauritius and T.a.auratus respectively. The amplification of D-Loop sequence by mean of PCR technique was performed using specific designed primers forward D-LoopRs 5’-GTACTTAACTCCACCACCAA-3’ and reverse D-LoopRs 5’-GTTGAGTTGGGTATGCTCGA-3’. The phylogenetic tree reconstruction based on MEGA 5 using Maximum Likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model shows that Ijem and Embun are placed in different clade. Pairwise distance from those samples value is 11,4%, it prove that they are interspecies. This result supports the morphological characteristic and previous research used Cyt-b sequence, Ijem is Trachypithecus auratus mauritius and Embun is Trachypithecus auratus auratus. Keywords : Identification, Javan Langur (Trachypithecus auratus), D-loop, Javan Langur Center
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Identifikasi Lutung Jawa (Trachypithecus auratus) di Javan Langur Center (JLC) Coban Talun, Batu berdasarkan Sekuen Cytochrome-b
SKRIPSI Jurusan Biologi - Fakultas MIPA UM, 2013Co-Authors: Rita Rizky RahmawatiAbstract:ABSTRAK Rahmawati, Rita Rizky. 2013. Identifikasi Lutung Jawa (Trachypithecus auratus) di Javan Langur Center (JLC) Coban Talun, Batu berdasarkan Sekuen Cytochrome-b. Skripsi, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Malang. Pembimbing: (I) Dra. Dwi Listyorini, M.Si, D.Sc, (II) Dr. H. Abdul Gofur, M.Si. Kata Kunci: Lutung Jawa, Javan Langur Center (JLC), Cytochrome-b Lutung Jawa (Trachypithecus auratus) merupakan lutung endemik Indonesia dan tergolong sebagai primata yang rentan (vulnerable). Beberapa peneliti membagi spesies Lutung Jawa (T. auratus) menjadi beberapa subspesies, namun tidak dijelaskan lebih lanjut mengenai batasan yang digunakan. Gen Cytochrome-b (Cyt-b) dari DNA mitokondria dapat digunakan sebagai indikator yang kuat untuk identifikasi spesies dan kajian evolusi molekuler. Tujuan penelitian ini adalah untuk mengidentifikasi subspesies Lutung Jawa (T. auratus) berdasarkan sekuen Cyt-b. Jenis penelitian ini adalah deskriptif eksploratif dengan sampel dua ekor Lutung Jawa (T. auratus) dari Javan Langur Center (JLC) yang bernama Andin (♀) dan Bobby (♂). Amplifikasi gen target Cyt-b dari DNA total darah dilakukan dengan teknik PCR menggunakan primer Forward L15162 5’-CTTCCATGAGGACAAATATC-3’ dan primer Reverse Rmuc1 5’-GTGGAGTATAGGTATGATTGC-3’. Rekonstruksi pohon filogenetik menggunakan software MEGA5 dengan metode maximum likelihood (ML), sedangkan analisis pairwise distance menggunakan model Kimura-2 parameter. Hasil analisis pohon filogenetik dan nilai pairwise distance diketahui bahwa Andin dan Bobby berada satu kelompok dengan T. a. auratus. Andin terletak satu clade dengan T. a. auratus haplotype aaF, sedangkan Bobby terletak satu clade dengan T. a. auratus dan T. a. auratus haplotype aaC. Berdasarkan analisis tersebut dapat disimpulkan bahwa kedua sampel termasuk dalam subspesies T. a. auratus (Lutung Jawa sebaran Jawa bagian Timur)
Chengming Huang - One of the best experts on this subject based on the ideXlab platform.
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Head and shoulder coloration of François’s langur (Trachypithecus francoisi; left), white-headed langur (T. leucocephalus; middle) and golden-headed langur (T. poliocephalus; right).
2013Co-Authors: Zhijin Liu, Tilo Nadler, Boshi Wang, Qihai Zhou, Chengming Huang, Guangjian Liu, Tao Sun, Jiang Zhou, Tengcheng Que, Ziming WangAbstract:Head and shoulder coloration of François’s langur (Trachypithecus francoisi; left), white-headed langur (T. leucocephalus; middle) and golden-headed langur (T. poliocephalus; right).
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ranging behavior of the francois langur Trachypithecus francoisi in limestone habitats of nonggang china
Integrative Zoology, 2011Co-Authors: Qihai Zhou, Chengming Huang, L I Ming, Fuwen WeiAbstract:Ranging behavior is an important aspect of animal behavior that researchers use to investigate ecological influences on individual behavior. We collected data on the ranging behavior of one group of Francois' langurs (Trachypithecus francoisi) between August 2003 and July 2004 in a limestone habitat within Nonggang Nature Reserve, China. We evaluated the influences of food availability, water resources and sleeping sites on ranging behavior. During the study period, the total home range size for the study group was 64.5 or (69.3 ha if the lacunae within the borders in which langurs were not observed were included), and the mean daily path length was 541 m. These are well within the range of variation reported in Trachypithecus species. The monthly range size was between 9.8 and 23.3 ha, and monthly range size correlated negatively with young leaf availability. The langurs tended to use a larger range size during young leaf-lean periods. The langurs did not use their home range uniformly, and 74% of their activities occurred within 35% of their home range. The most heavily used quadrats in the home range were located near the most frequently used sleeping sites, suggesting that sleeping sites have a significant influence on the ranging behavior of Francois' langurs in limestone habitats.
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factors affecting the ranging behavior of white headed langurs Trachypithecus leucocephalus
International Journal of Primatology, 2011Co-Authors: Qihai Zhou, Chengming Huang, Xiaoping Tang, Henglian HuangAbstract:Ranging behavior is an important aspect of animal behavior that researchers use to investigate ecological influences on individual behavior. We studied the influence of diet, water resources, and sleeping sites on the ranging behavior of 2 groups of white-headed langurs (Trachypithecus leucocephalus) in a limestone habitat at Fusui Nature Reserve, China, between August 2007 and July 2008. During the study period, the total home range sizes for the 2 focal groups were 23.8 ha and 33.8 ha, the mean daily path lengths were 491 m and 512 m, and leaves accounted for 83.4% and 91.0% of the diet, which are well within the range of variation reported for other Trachypithecus. One focal group traveled significantly longer distances in the rainy season months than in the dry season months. This variation may be related to the seasonal difference in food availability and diet. The langurs did not use their home ranges uniformly, and 50% of their activities occurred within 11% (group 1) and 20% (group 2) of their home ranges. The most heavily used quadrats in the home ranges were located near the most frequently used sleeping sites. Moreover, the core areas (>70% of location records) of both groups’ home ranges included ≥1 permanent water pool. The langurs ventured to these pools for drinking when surface water became scarce in the dry season. These results suggest that sleeping sites and water scarcity may be significant influences on the ranging behavior of white-headed langurs in limestone habitat.
Dwi Listyorini - One of the best experts on this subject based on the ideXlab platform.
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identification of javan langur Trachypithecus auratus in javan langur center jlc coban talun batu based on cytochrome b sequence
KnE Life Sciences, 2015Co-Authors: Rita Rizky Rahmawati, Emy Kusumawati, Lulus Putri Aninda, Miftahul Mushlih, Abdul Gofur, Dwi ListyoriniAbstract:Javan Langur (Trachypithecus auratus) is one of endemic primates in Indonesia and classified into vulnerable level. Based on its morphological characteristic and geographic area, Javan Langur divided into 2 subspecies, those are Trachypithecus auratus auratus and Trachypithecus auratus mauritius. The identification of its morphological characteristic and genetic in species or subspecies level is important before releasing because it is correlated with inbreeding grouping and releasing place. The aim of this study is to identify the Javan Langur (Trachypithecus auratus) in Javan Langur Center (JLC) Coban Talun-Batu based on D-Loop sequence. The blood sample used in this study was taken from two adult female Javan Langur named Ijem and Embun, that have different morphological characteristics. Both (primates) are suggested to be T.a.mauritius and T.a.auratus respectively. The amplification of D-Loop sequence by mean of PCR technique was performed using specific designed primers forward D-LoopRs 5’-GTACTTAACTCCACCACCAA-3’ and reverse D-LoopRs 5’-GTTGAGTTGGGTATGCTCGA-3’. The phylogenetic tree reconstruction based on MEGA 5 using Maximum Likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model shows that Ijem and Embun are placed in different clade. Pairwise distance from those samples value is 11,4%, it prove that they are interspecies. This result supports the morphological characteristic and previous research used Cyt-b sequence, Ijem is Trachypithecus auratus mauritius and Embun is Trachypithecus auratus auratus. Keywords : Identification, Javan Langur (Trachypithecus auratus), D-loop, Javan Langur Center
Qihai Zhou - One of the best experts on this subject based on the ideXlab platform.
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Head and shoulder coloration of François’s langur (Trachypithecus francoisi; left), white-headed langur (T. leucocephalus; middle) and golden-headed langur (T. poliocephalus; right).
2013Co-Authors: Zhijin Liu, Tilo Nadler, Boshi Wang, Qihai Zhou, Chengming Huang, Guangjian Liu, Tao Sun, Jiang Zhou, Tengcheng Que, Ziming WangAbstract:Head and shoulder coloration of François’s langur (Trachypithecus francoisi; left), white-headed langur (T. leucocephalus; middle) and golden-headed langur (T. poliocephalus; right).
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ranging behavior of the francois langur Trachypithecus francoisi in limestone habitats of nonggang china
Integrative Zoology, 2011Co-Authors: Qihai Zhou, Chengming Huang, L I Ming, Fuwen WeiAbstract:Ranging behavior is an important aspect of animal behavior that researchers use to investigate ecological influences on individual behavior. We collected data on the ranging behavior of one group of Francois' langurs (Trachypithecus francoisi) between August 2003 and July 2004 in a limestone habitat within Nonggang Nature Reserve, China. We evaluated the influences of food availability, water resources and sleeping sites on ranging behavior. During the study period, the total home range size for the study group was 64.5 or (69.3 ha if the lacunae within the borders in which langurs were not observed were included), and the mean daily path length was 541 m. These are well within the range of variation reported in Trachypithecus species. The monthly range size was between 9.8 and 23.3 ha, and monthly range size correlated negatively with young leaf availability. The langurs tended to use a larger range size during young leaf-lean periods. The langurs did not use their home range uniformly, and 74% of their activities occurred within 35% of their home range. The most heavily used quadrats in the home range were located near the most frequently used sleeping sites, suggesting that sleeping sites have a significant influence on the ranging behavior of Francois' langurs in limestone habitats.
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factors affecting the ranging behavior of white headed langurs Trachypithecus leucocephalus
International Journal of Primatology, 2011Co-Authors: Qihai Zhou, Chengming Huang, Xiaoping Tang, Henglian HuangAbstract:Ranging behavior is an important aspect of animal behavior that researchers use to investigate ecological influences on individual behavior. We studied the influence of diet, water resources, and sleeping sites on the ranging behavior of 2 groups of white-headed langurs (Trachypithecus leucocephalus) in a limestone habitat at Fusui Nature Reserve, China, between August 2007 and July 2008. During the study period, the total home range sizes for the 2 focal groups were 23.8 ha and 33.8 ha, the mean daily path lengths were 491 m and 512 m, and leaves accounted for 83.4% and 91.0% of the diet, which are well within the range of variation reported for other Trachypithecus. One focal group traveled significantly longer distances in the rainy season months than in the dry season months. This variation may be related to the seasonal difference in food availability and diet. The langurs did not use their home ranges uniformly, and 50% of their activities occurred within 11% (group 1) and 20% (group 2) of their home ranges. The most heavily used quadrats in the home ranges were located near the most frequently used sleeping sites. Moreover, the core areas (>70% of location records) of both groups’ home ranges included ≥1 permanent water pool. The langurs ventured to these pools for drinking when surface water became scarce in the dry season. These results suggest that sleeping sites and water scarcity may be significant influences on the ranging behavior of white-headed langurs in limestone habitat.
Miftahul Mushlih - One of the best experts on this subject based on the ideXlab platform.
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identification of javan langur Trachypithecus auratus in javan langur center jlc coban talun batu based on cytochrome b sequence
KnE Life Sciences, 2015Co-Authors: Rita Rizky Rahmawati, Emy Kusumawati, Lulus Putri Aninda, Miftahul Mushlih, Abdul Gofur, Dwi ListyoriniAbstract:Javan Langur (Trachypithecus auratus) is one of endemic primates in Indonesia and classified into vulnerable level. Based on its morphological characteristic and geographic area, Javan Langur divided into 2 subspecies, those are Trachypithecus auratus auratus and Trachypithecus auratus mauritius. The identification of its morphological characteristic and genetic in species or subspecies level is important before releasing because it is correlated with inbreeding grouping and releasing place. The aim of this study is to identify the Javan Langur (Trachypithecus auratus) in Javan Langur Center (JLC) Coban Talun-Batu based on D-Loop sequence. The blood sample used in this study was taken from two adult female Javan Langur named Ijem and Embun, that have different morphological characteristics. Both (primates) are suggested to be T.a.mauritius and T.a.auratus respectively. The amplification of D-Loop sequence by mean of PCR technique was performed using specific designed primers forward D-LoopRs 5’-GTACTTAACTCCACCACCAA-3’ and reverse D-LoopRs 5’-GTTGAGTTGGGTATGCTCGA-3’. The phylogenetic tree reconstruction based on MEGA 5 using Maximum Likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model shows that Ijem and Embun are placed in different clade. Pairwise distance from those samples value is 11,4%, it prove that they are interspecies. This result supports the morphological characteristic and previous research used Cyt-b sequence, Ijem is Trachypithecus auratus mauritius and Embun is Trachypithecus auratus auratus. Keywords : Identification, Javan Langur (Trachypithecus auratus), D-loop, Javan Langur Center
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Kekerabatan Lutung Jawa Di Pusat Konservasi Lutung Jawa, Kecamatan Dau, Kabupaten Malang, Berdasarkan Sekuen Gen Cytochrome B.
SKRIPSI Jurusan Biologi - Fakultas MIPA UM, 2011Co-Authors: Miftahul MushlihAbstract:ABSTRAK Lutung Jawa (Trachypithecus auratus) merupakan salah satu spesies endemik Indonesia yang keberadaannnya dinyatakan rentan (vurnerable) terhadap kepunahan oleh International Union for Conservation of Nature and Natural(IUCN). Di Pusat Konservasi Lutung Jawa, Kecamatan Dau, Kabupaten Resources Malang, diduga ada dua subspesies yaitu Trachypithecus auratus auratus (bernama Rus) dan Trachypithecus auratus mauritius (Bernama Ijem). Identifikasi kekerabatan Lutung Jawa yang selama ini dilakukan di Pusat Konservasi Lutung Jawa terbatas pada pengamatan morfologi dan daerah asalnya saja, sehingga menghasilkan informasi yang terbatas. Analisis menggunakan DNA dianggap mampu menghasilkan informasi yang lebih akurat. Identifikasi kekerabatan Lutung Jawa diperlukan untuk memetakan kelompok Lutung Jawa sebelum dikembalikan kehabitat asalnya. Dari hasil Polymerase Chain Reaction (PCR) menggunakan primer Forward hasil modifikasi dari L15162 (Wangchuk, 2005), 5’-CTTCC ATGAGGACAAATATC-3’ dan Primer Reverse Rmuc1, 5’-GTGGAGTATAGGT ATGATTGC-3’ diperoleh sekuen gen cyt-b sepanjang 500 bp pada Rus dan 501 bp pada Ijem. Hasil analisis topologi filogenetik dengan metode Maximum-likelihood (ML) dan pairwise distance dengan menggunakan software MEGA5 menunjukkan bahwa Rus dan Ijem berkerabat sangat dekat dan merupakan satu jenis T. a.. Nilai pairwise distance kedua sampel 0,4% ± 0,0027. Topologi pohon auratus filogenetik menggunakan metode ML menunjukkan kedua sampel berkerabat dekat dengan T. a. auratus haplotype aaJ ǀEF465117.1ǀ, T. a. auratus haplotype aaG ǀ EF465115.1ǀ, dan T. a. auratus haplotype aaB ǀEF465110.1ǀ, serta berada dalam satu kelompok dengan anggota T. auratus lainnya
