The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform
Catherine Porcher - One of the best experts on this subject based on the ideXlab platform.
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ETV6 activates vegfa expression through positive and negative Transcriptional regulatory networks in Xenopus embryos
Nature Communications, 2019Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling controls physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here, we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits expression of the repressor foxo3 , to prevent Foxo3 from binding to and repressing the vegfa promoter. ETV6 also directly activates expression of the activator klf4 ; reflecting a genome-wide paucity in ETS-binding motifs in ETV6 genomic targets, Klf4 then recruits ETV6 to the vegfa promoter to activate its expression. These two mechanisms (double negative gate and feed-forward loop) are classic features of gene regulatory networks specifying cell fates. Thus, ETV6’s dual function, as a Transcriptional repressor and activator, controls a major signaling pathway involved in endothelial and blood development in vivo. How vegfa expression is Transcriptionally regulated in vivo is unclear. Here, the authors demonstrate that the ETS Transcription Factor ETV6 acts as a repressor and an activator of two direct regulators of vegfa expression ( foxo3 and klf4 , respectively) to control blood formation in Xenopus .
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Vegfa expression is activated through positive and negative Transcriptional regulatory networks controlled by the ETS Factor ETV6 in vivo
bioRxiv, 2018Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling is crucial for physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits the expression of the vegfa repressor, foxo3. Surprisingly, it also directly activates the expression of the vegfa activator, klf4. Finally, it indirectly binds to the vegfa promoter where it co-localizes with Klf4. Klf4 deficiency downregulates vegfa expression and significantly decreases ETV6 binding to the vegfa promoter, indicating that Klf4 recruits ETV6 to the vegfa promoter. Thus, our work uncovers a dual function for ETV6, as both a Transcriptional repressor and activator, in controlling a major signaling pathway involved in blood and endothelial development in vivo. Given the established relationships between development and cancer, this elaborate gene regulatory network may inform new strategies for the treatment of VEGFA-dependent tumorigenesis.
Lei Li - One of the best experts on this subject based on the ideXlab platform.
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ETV6 activates vegfa expression through positive and negative Transcriptional regulatory networks in Xenopus embryos
Nature Communications, 2019Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling controls physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here, we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits expression of the repressor foxo3 , to prevent Foxo3 from binding to and repressing the vegfa promoter. ETV6 also directly activates expression of the activator klf4 ; reflecting a genome-wide paucity in ETS-binding motifs in ETV6 genomic targets, Klf4 then recruits ETV6 to the vegfa promoter to activate its expression. These two mechanisms (double negative gate and feed-forward loop) are classic features of gene regulatory networks specifying cell fates. Thus, ETV6’s dual function, as a Transcriptional repressor and activator, controls a major signaling pathway involved in endothelial and blood development in vivo. How vegfa expression is Transcriptionally regulated in vivo is unclear. Here, the authors demonstrate that the ETS Transcription Factor ETV6 acts as a repressor and an activator of two direct regulators of vegfa expression ( foxo3 and klf4 , respectively) to control blood formation in Xenopus .
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Vegfa expression is activated through positive and negative Transcriptional regulatory networks controlled by the ETS Factor ETV6 in vivo
bioRxiv, 2018Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling is crucial for physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits the expression of the vegfa repressor, foxo3. Surprisingly, it also directly activates the expression of the vegfa activator, klf4. Finally, it indirectly binds to the vegfa promoter where it co-localizes with Klf4. Klf4 deficiency downregulates vegfa expression and significantly decreases ETV6 binding to the vegfa promoter, indicating that Klf4 recruits ETV6 to the vegfa promoter. Thus, our work uncovers a dual function for ETV6, as both a Transcriptional repressor and activator, in controlling a major signaling pathway involved in blood and endothelial development in vivo. Given the established relationships between development and cancer, this elaborate gene regulatory network may inform new strategies for the treatment of VEGFA-dependent tumorigenesis.
Rossella Rispoli - One of the best experts on this subject based on the ideXlab platform.
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ETV6 activates vegfa expression through positive and negative Transcriptional regulatory networks in Xenopus embryos
Nature Communications, 2019Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling controls physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here, we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits expression of the repressor foxo3 , to prevent Foxo3 from binding to and repressing the vegfa promoter. ETV6 also directly activates expression of the activator klf4 ; reflecting a genome-wide paucity in ETS-binding motifs in ETV6 genomic targets, Klf4 then recruits ETV6 to the vegfa promoter to activate its expression. These two mechanisms (double negative gate and feed-forward loop) are classic features of gene regulatory networks specifying cell fates. Thus, ETV6’s dual function, as a Transcriptional repressor and activator, controls a major signaling pathway involved in endothelial and blood development in vivo. How vegfa expression is Transcriptionally regulated in vivo is unclear. Here, the authors demonstrate that the ETS Transcription Factor ETV6 acts as a repressor and an activator of two direct regulators of vegfa expression ( foxo3 and klf4 , respectively) to control blood formation in Xenopus .
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Vegfa expression is activated through positive and negative Transcriptional regulatory networks controlled by the ETS Factor ETV6 in vivo
bioRxiv, 2018Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling is crucial for physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits the expression of the vegfa repressor, foxo3. Surprisingly, it also directly activates the expression of the vegfa activator, klf4. Finally, it indirectly binds to the vegfa promoter where it co-localizes with Klf4. Klf4 deficiency downregulates vegfa expression and significantly decreases ETV6 binding to the vegfa promoter, indicating that Klf4 recruits ETV6 to the vegfa promoter. Thus, our work uncovers a dual function for ETV6, as both a Transcriptional repressor and activator, in controlling a major signaling pathway involved in blood and endothelial development in vivo. Given the established relationships between development and cancer, this elaborate gene regulatory network may inform new strategies for the treatment of VEGFA-dependent tumorigenesis.
Roger Patient - One of the best experts on this subject based on the ideXlab platform.
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ETV6 activates vegfa expression through positive and negative Transcriptional regulatory networks in Xenopus embryos
Nature Communications, 2019Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling controls physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here, we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits expression of the repressor foxo3 , to prevent Foxo3 from binding to and repressing the vegfa promoter. ETV6 also directly activates expression of the activator klf4 ; reflecting a genome-wide paucity in ETS-binding motifs in ETV6 genomic targets, Klf4 then recruits ETV6 to the vegfa promoter to activate its expression. These two mechanisms (double negative gate and feed-forward loop) are classic features of gene regulatory networks specifying cell fates. Thus, ETV6’s dual function, as a Transcriptional repressor and activator, controls a major signaling pathway involved in endothelial and blood development in vivo. How vegfa expression is Transcriptionally regulated in vivo is unclear. Here, the authors demonstrate that the ETS Transcription Factor ETV6 acts as a repressor and an activator of two direct regulators of vegfa expression ( foxo3 and klf4 , respectively) to control blood formation in Xenopus .
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Vegfa expression is activated through positive and negative Transcriptional regulatory networks controlled by the ETS Factor ETV6 in vivo
bioRxiv, 2018Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling is crucial for physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits the expression of the vegfa repressor, foxo3. Surprisingly, it also directly activates the expression of the vegfa activator, klf4. Finally, it indirectly binds to the vegfa promoter where it co-localizes with Klf4. Klf4 deficiency downregulates vegfa expression and significantly decreases ETV6 binding to the vegfa promoter, indicating that Klf4 recruits ETV6 to the vegfa promoter. Thus, our work uncovers a dual function for ETV6, as both a Transcriptional repressor and activator, in controlling a major signaling pathway involved in blood and endothelial development in vivo. Given the established relationships between development and cancer, this elaborate gene regulatory network may inform new strategies for the treatment of VEGFA-dependent tumorigenesis.
Aldo Ciau-uitz - One of the best experts on this subject based on the ideXlab platform.
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ETV6 activates vegfa expression through positive and negative Transcriptional regulatory networks in Xenopus embryos
Nature Communications, 2019Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling controls physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here, we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits expression of the repressor foxo3 , to prevent Foxo3 from binding to and repressing the vegfa promoter. ETV6 also directly activates expression of the activator klf4 ; reflecting a genome-wide paucity in ETS-binding motifs in ETV6 genomic targets, Klf4 then recruits ETV6 to the vegfa promoter to activate its expression. These two mechanisms (double negative gate and feed-forward loop) are classic features of gene regulatory networks specifying cell fates. Thus, ETV6’s dual function, as a Transcriptional repressor and activator, controls a major signaling pathway involved in endothelial and blood development in vivo. How vegfa expression is Transcriptionally regulated in vivo is unclear. Here, the authors demonstrate that the ETS Transcription Factor ETV6 acts as a repressor and an activator of two direct regulators of vegfa expression ( foxo3 and klf4 , respectively) to control blood formation in Xenopus .
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Vegfa expression is activated through positive and negative Transcriptional regulatory networks controlled by the ETS Factor ETV6 in vivo
bioRxiv, 2018Co-Authors: Lei Li, Rossella Rispoli, Roger Patient, Aldo Ciau-uitz, Catherine PorcherAbstract:VEGFA signaling is crucial for physiological and pathological angiogenesis and hematopoiesis. Although many context-dependent signaling pathways downstream of VEGFA have been uncovered, vegfa Transcriptional regulation in vivo remains unclear. Here we show that the ETS Transcription Factor, ETV6, positively regulates vegfa expression during Xenopus blood stem cell development through multiple Transcriptional inputs. In agreement with its established repressive functions, ETV6 directly inhibits the expression of the vegfa repressor, foxo3. Surprisingly, it also directly activates the expression of the vegfa activator, klf4. Finally, it indirectly binds to the vegfa promoter where it co-localizes with Klf4. Klf4 deficiency downregulates vegfa expression and significantly decreases ETV6 binding to the vegfa promoter, indicating that Klf4 recruits ETV6 to the vegfa promoter. Thus, our work uncovers a dual function for ETV6, as both a Transcriptional repressor and activator, in controlling a major signaling pathway involved in blood and endothelial development in vivo. Given the established relationships between development and cancer, this elaborate gene regulatory network may inform new strategies for the treatment of VEGFA-dependent tumorigenesis.