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Mutsumi Nishida - One of the best experts on this subject based on the ideXlab platform.
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organization of the mitochondrial genome of a deep sea fish gonostoma gracile teleostei stomiiformes first example of Transfer RNA gene rearrangements in bony fishes
Marine Biotechnology, 1999Co-Authors: Masaki Miya, Mutsumi NishidaAbstract:We determined the complete nucleotide sequence of the mitochondrial genome (except for a portion of the putative control region) for a deep-sea fish, Gonostoma gracile. The entire mitochondrial genome was purified by gene amplification using long polymerase chain reaction (long PCR), and the products were subsequently used as templates for PCR with 30 sets of newly designed, fish-universal primers that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products showed that the genome contained the same 37 mitochondrial structural genes as found in other vertebrates (two ribosomal RNA, 22 Transfer RNA, and 13 protein-coding genes), with the order of all rRNA and protein-coding genes, and 19 tRNA genes being identical to that in typical vertebrates. The gene order of the three tRNAs (tRNA Glu , tRNA Thr , and tRNA Pro ) relative to cytochrome b, however, differed from that determined in other vertebrates.
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organization of the mitochondrial genome of a deep sea fish gonostoma gracile teleostei stomiiformes first example of Transfer RNA gene rearrangements in bony fishes
Marine Biotechnology, 1999Co-Authors: Masaki Miya, Mutsumi NishidaAbstract:We determined the complete nucleotide sequence of the mitochondrial genome (except for a portion of the putative control region) for a deep-sea fish, Gonostoma gracile. The entire mitochondrial genome was purified by gene amplification using long polymerase chain reaction (long PCR), and the products were subsequently used as templates for PCR with 30 sets of newly designed, fish-universal primers that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products showed that the genome contained the same 37 mitochondrial structural genes as found in other vertebrates (two ribosomal RNA, 22 Transfer RNA, and 13 protein-coding genes), with the order of all rRNA and protein-coding genes, and 19 tRNA genes being identical to that in typical vertebrates. The gene order of the three tRNAs (tRNAGlu, tRNAThr, and tRNAPro) relative to cytochrome b, however, differed from that determined in other vertebrates. Two steps of tandem duplication of gene regions, each followed by deletions of genes, can be invoked as mechanisms generating such rearrangements of tRNAs. This is the first example of tRNA gene rearrangements in a bony fish mitochondrial genome.
Masaki Miya - One of the best experts on this subject based on the ideXlab platform.
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organization of the mitochondrial genome of a deep sea fish gonostoma gracile teleostei stomiiformes first example of Transfer RNA gene rearrangements in bony fishes
Marine Biotechnology, 1999Co-Authors: Masaki Miya, Mutsumi NishidaAbstract:We determined the complete nucleotide sequence of the mitochondrial genome (except for a portion of the putative control region) for a deep-sea fish, Gonostoma gracile. The entire mitochondrial genome was purified by gene amplification using long polymerase chain reaction (long PCR), and the products were subsequently used as templates for PCR with 30 sets of newly designed, fish-universal primers that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products showed that the genome contained the same 37 mitochondrial structural genes as found in other vertebrates (two ribosomal RNA, 22 Transfer RNA, and 13 protein-coding genes), with the order of all rRNA and protein-coding genes, and 19 tRNA genes being identical to that in typical vertebrates. The gene order of the three tRNAs (tRNA Glu , tRNA Thr , and tRNA Pro ) relative to cytochrome b, however, differed from that determined in other vertebrates.
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organization of the mitochondrial genome of a deep sea fish gonostoma gracile teleostei stomiiformes first example of Transfer RNA gene rearrangements in bony fishes
Marine Biotechnology, 1999Co-Authors: Masaki Miya, Mutsumi NishidaAbstract:We determined the complete nucleotide sequence of the mitochondrial genome (except for a portion of the putative control region) for a deep-sea fish, Gonostoma gracile. The entire mitochondrial genome was purified by gene amplification using long polymerase chain reaction (long PCR), and the products were subsequently used as templates for PCR with 30 sets of newly designed, fish-universal primers that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products showed that the genome contained the same 37 mitochondrial structural genes as found in other vertebrates (two ribosomal RNA, 22 Transfer RNA, and 13 protein-coding genes), with the order of all rRNA and protein-coding genes, and 19 tRNA genes being identical to that in typical vertebrates. The gene order of the three tRNAs (tRNAGlu, tRNAThr, and tRNAPro) relative to cytochrome b, however, differed from that determined in other vertebrates. Two steps of tandem duplication of gene regions, each followed by deletions of genes, can be invoked as mechanisms generating such rearrangements of tRNAs. This is the first example of tRNA gene rearrangements in a bony fish mitochondrial genome.
I N Targoff - One of the best experts on this subject based on the ideXlab platform.
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clinical and immunogenetic features of patients with autoantibodies to asparaginyl Transfer RNA synthetase
Arthritis & Rheumatism, 2007Co-Authors: Michito Hirakata, Akira Suwa, Sonoko Nagai, Tsuneyo Mimori, Tetsuya Takada, Shinji Sato, Ekkehard Genth, Yeong W Song, I N TargoffAbstract:Objective We have previously described anti-KS autoantibodies and provided evidence that they are directed against asparaginyl–Transfer RNA (tRNA) synthetase (AsnRS). The aim of the present study was to identify patients with anti-AsnRS autoantibodies and elucidate the clinical significance of this sixth antisynthetase antibody. In particular, we studied whether it was associated with the syndrome of myositis (polymyositis or dermatomyositis [DM]), interstitial lung disease (ILD), arthritis, and other features that had been previously associated with the 5 other anti–aminoacyl–tRNA synthetase autoantibodies. Methods More than 2,500 sera from patients with connective tissue disease (including myositis and ILD) and controls were examined for anti-AsnRS autoantibodies by immunoprecipitation (IP). Positive and control sera were tested for the ability to inhibit AsnRS by preincubation of the enzyme source with the serum. The HLA class II (DRB1, DQA1, DQB1, DPB1) alleles were identified from restriction fragment length polymorphism of polymerase chain reaction–amplified genomic DNA. Results Anti-AsnRS antibodies were identified in the sera of 8 patients (5 Japanese, 1 American, 1 German, and 1 Korean) by IP of the same distinctive set of tRNA and protein that differed from those precipitated by the other 5 antisynthetases, and these antibodies showed specific inhibition of AsnRS activity. Two of these patients had DM, but 7 of 8 (88%) had ILD. Four patients (50%) had arthritis, and 1 had Raynaud's phenomenon. This antisynthetase was very rare among myositis patients (present in 0% of Japanese myositis patients), but it was found in 3% of Japanese ILD patients. Thus, most patients with anti-AsnRS had chronic ILD with or without features of connective tissue disease. Interestingly, all 4 Japanese patients tested had DR2 (DRB1*1501/1502), compared with 33% of healthy controls. Conclusion These results indicate that anti-AsnRS autoantibodies, like anti–alanyl–tRNA synthetase autoantibodies, have a stronger association with ILD than with myositis and may be associated with the DR2 phenotype.
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anti ks identification of autoantibodies to asparaginyl Transfer RNA synthetase associated with interstitial lung disease
Journal of Immunology, 1999Co-Authors: Michito Hirakata, Akira Suwa, Sonoko Nagai, Michael A Kron, Edward P Trieu, Tsuneyo Mimori, Masashi Akizuki, I N TargoffAbstract:Autoantibodies to five of the aminoacyl-Transfer RNA (tRNA) synthetases have been described, and each is associated with a syndrome of inflammatory myopathy with interstitial lung disease (ILD) and arthritis. Serum KS, from a patient with ILD and inflammatory arthritis without evidence of myositis, immunoprecipitated a tRNA that was distinct from that precipitated by any described anti-synthetase or other reported tRNA-related Abs, along with a protein of 65 kDa. KS serum and IgG fraction each showed significant (88%) inhibition of asparaginyl-tRNA synthetase (AsnRS) activity, but not of any of the other 19 aminoacyl-tRNA synthetase activities. Among 884 patients with connective tissue diseases tested, only two other sera were found to immunoprecipitate tRNAs and proteins of identical gel mobility. These two and KS showed identical immunodiffusion lines using HeLa cell extract. The new sera significantly inhibited AsnRS without significant effects on other synthetases tested. Both patients had ILD but neither had evidence of myositis. These data strongly suggest that these three sera have autoantibodies to AsnRS, representing a sixth anti-synthetase. Anti-KS was more closely associated with ILD than with myositis. Further study of this Abs might prove useful in dissecting the stimuli responsible for the genesis of anti-synthetase autoantibodies.
Michito Hirakata - One of the best experts on this subject based on the ideXlab platform.
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clinical and immunogenetic features of patients with autoantibodies to asparaginyl Transfer RNA synthetase
Arthritis & Rheumatism, 2007Co-Authors: Michito Hirakata, Akira Suwa, Sonoko Nagai, Tsuneyo Mimori, Tetsuya Takada, Shinji Sato, Ekkehard Genth, Yeong W Song, I N TargoffAbstract:Objective We have previously described anti-KS autoantibodies and provided evidence that they are directed against asparaginyl–Transfer RNA (tRNA) synthetase (AsnRS). The aim of the present study was to identify patients with anti-AsnRS autoantibodies and elucidate the clinical significance of this sixth antisynthetase antibody. In particular, we studied whether it was associated with the syndrome of myositis (polymyositis or dermatomyositis [DM]), interstitial lung disease (ILD), arthritis, and other features that had been previously associated with the 5 other anti–aminoacyl–tRNA synthetase autoantibodies. Methods More than 2,500 sera from patients with connective tissue disease (including myositis and ILD) and controls were examined for anti-AsnRS autoantibodies by immunoprecipitation (IP). Positive and control sera were tested for the ability to inhibit AsnRS by preincubation of the enzyme source with the serum. The HLA class II (DRB1, DQA1, DQB1, DPB1) alleles were identified from restriction fragment length polymorphism of polymerase chain reaction–amplified genomic DNA. Results Anti-AsnRS antibodies were identified in the sera of 8 patients (5 Japanese, 1 American, 1 German, and 1 Korean) by IP of the same distinctive set of tRNA and protein that differed from those precipitated by the other 5 antisynthetases, and these antibodies showed specific inhibition of AsnRS activity. Two of these patients had DM, but 7 of 8 (88%) had ILD. Four patients (50%) had arthritis, and 1 had Raynaud's phenomenon. This antisynthetase was very rare among myositis patients (present in 0% of Japanese myositis patients), but it was found in 3% of Japanese ILD patients. Thus, most patients with anti-AsnRS had chronic ILD with or without features of connective tissue disease. Interestingly, all 4 Japanese patients tested had DR2 (DRB1*1501/1502), compared with 33% of healthy controls. Conclusion These results indicate that anti-AsnRS autoantibodies, like anti–alanyl–tRNA synthetase autoantibodies, have a stronger association with ILD than with myositis and may be associated with the DR2 phenotype.
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anti ks identification of autoantibodies to asparaginyl Transfer RNA synthetase associated with interstitial lung disease
Journal of Immunology, 1999Co-Authors: Michito Hirakata, Akira Suwa, Sonoko Nagai, Michael A Kron, Edward P Trieu, Tsuneyo Mimori, Masashi Akizuki, I N TargoffAbstract:Autoantibodies to five of the aminoacyl-Transfer RNA (tRNA) synthetases have been described, and each is associated with a syndrome of inflammatory myopathy with interstitial lung disease (ILD) and arthritis. Serum KS, from a patient with ILD and inflammatory arthritis without evidence of myositis, immunoprecipitated a tRNA that was distinct from that precipitated by any described anti-synthetase or other reported tRNA-related Abs, along with a protein of 65 kDa. KS serum and IgG fraction each showed significant (88%) inhibition of asparaginyl-tRNA synthetase (AsnRS) activity, but not of any of the other 19 aminoacyl-tRNA synthetase activities. Among 884 patients with connective tissue diseases tested, only two other sera were found to immunoprecipitate tRNAs and proteins of identical gel mobility. These two and KS showed identical immunodiffusion lines using HeLa cell extract. The new sera significantly inhibited AsnRS without significant effects on other synthetases tested. Both patients had ILD but neither had evidence of myositis. These data strongly suggest that these three sera have autoantibodies to AsnRS, representing a sixth anti-synthetase. Anti-KS was more closely associated with ILD than with myositis. Further study of this Abs might prove useful in dissecting the stimuli responsible for the genesis of anti-synthetase autoantibodies.
Jesus Torresvazquez - One of the best experts on this subject based on the ideXlab platform.
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aminoacyl Transfer RNA synthetase deficiency promotes angiogenesis via the unfolded protein response pathway
Arteriosclerosis Thrombosis and Vascular Biology, 2016Co-Authors: Daniel Castranova, Paul J Paukstelis, Matthew R Swift, Brigid D Lo, Andrew E Davis, Van N Pham, Mayumi F Miller, Jesus TorresvazquezAbstract:Objective—Understanding the mechanisms regulating normal and pathological angiogenesis is of great scientific and clinical interest. In this report, we show that mutations in 2 different aminoacyl-Transfer RNA synthetases, threonyl tRNA synthetase (tarsy58) or isoleucyl tRNA synthetase (iarsy68), lead to similar increased branching angiogenesis in developing zebrafish. Approach and Results—The unfolded protein response pathway is activated by aminoacyl-Transfer RNA synthetase deficiencies, and we show that unfolded protein response genes atf4, atf6, and xbp1, as well as the key proangiogenic ligand vascular endothelial growth factor (vegfaa), are all upregulated in tarsy58 and iarsy68 mutants. Finally, we show that the protein kinase RNA-like endoplasmic reticulum kinase–activating transcription factor 4 arm of the unfolded protein response pathway is necessary for both the elevated vegfaa levels and increased angiogenesis observed in tarsy58 mutants. Conclusions—Our results suggest that endoplasmic retic...
