The Experts below are selected from a list of 74814 Experts worldwide ranked by ideXlab platform
Sanfeng Zhang - One of the best experts on this subject based on the ideXlab platform.
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Political incentives, Transformation Efficiency and resource-exhausted cities
Journal of Cleaner Production, 2018Co-Authors: Huiming Zhang, Lifang Xiong, Sanfeng ZhangAbstract:Abstract Since 2003, the evaluation system of government officials in China has undergone significant changes from relying on almost solely on traditional economic growth criteria to a broader set of indicators including environmental quality and per-capita disposable income of urban residents. Resource-exhausted cities face severe problems, such as environmental pollution and industrial restructuring, during this process of city Transformation. While addressing these problems, local officials, especially local top leaders, play a very important role, because their performance under this new evaluation system, to a certain extent, impacts the Transformation Efficiency of resource-exhausted cities. Based on this, we analyze the relationship between political incentives and city Transformation Efficiency by applying a panel dataset of 37 resource-exhausted cities from the 2004–2014. The findings reveal that: (1) Transformation Efficiency has been improved noticeably over the final two years of the sample period following 8 years of fluctuation without meaningful improvement. (2) Of the factors which impact the Transformation Efficiency of resource-exhausted cities, foreign direct investment, fiscal deficit and number of internet users produce only weak effects. This indicates that investment openness, fiscal surplus of local government, and infrastructure construction are not key variables in boosting urban Transformation. (3) The impact of political incentives on the Transformation Efficiency of resource-exhausted cities depends on whether the officials are municipal party secretaries or mayors. The results confirm that the existing authority structure in China has an effect on the Transformation of resource-exhausted cities and imply that, in contrast to the municipal party secretaries, the mayors take a subordinate position in the authority structure, thus failing to exert their effective role in the process of urban Transformation.
Thi Duc Thai - One of the best experts on this subject based on the ideXlab platform.
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Identification of a cytosine methyltransferase that improves Transformation Efficiency in Methylomonas sp. DH-1
Biotechnology for Biofuels, 2020Co-Authors: Jun Ren, Hyang-mi Lee, Thi Duc ThaiAbstract:Background Industrial biofuels and other value-added products can be produced from metabolically engineered microorganisms. Methylomonas sp. DH-1 is a candidate platform for bioconversion that uses methane as a carbon source. Although several genetic engineering techniques have been developed to work with Methylomonas sp. DH-1, the genetic manipulation of plasmids remains difficult because of the restriction-modification (RM) system present in the bacteria. Therefore, the RM system in Methylomonas sp. DH-1 must be identified to improve the genetic engineering prospects of this microorganism. Results We identified a DNA methylation site, TGGCCA, and its corresponding cytosine methyltransferase for the first time in Methylomonas sp. DH-1 through whole-genome bisulfite sequencing. The methyltransferase was confirmed to methylate the fourth nucleotide of TGG C CA. In general, methylated plasmids exhibited better Transformation Efficiency under the protection of the RM system than non-methylated plasmids did. As expected, when we transformed Methylomonas sp. DH-1 with plasmid DNA harboring the psy gene, the metabolic flux towards carotenoid increased. The methyltransferase-treated plasmid exhibited an increase in Transformation Efficiency of 2.5 × 10^3 CFU/μg (124%). The introduced gene increased the production of carotenoid by 26%. In addition, the methyltransferase-treated plasmid harboring anti- psy sRNA gene exhibited an increase in Transformation Efficiency by 70% as well. The production of carotenoid was decreased by 40% when the psy gene was translationally repressed by anti- psy sRNA. Conclusions Plasmid DNA methylated by the discovered cytosine methyltransferase from Methylomonas sp. DH-1 had a higher Transformation Efficiency than non-treated plasmid DNA. The RM system identified in this study may facilitate the plasmid-based genetic manipulation of methanotrophs.
Huiming Zhang - One of the best experts on this subject based on the ideXlab platform.
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Political incentives, Transformation Efficiency and resource-exhausted cities
Journal of Cleaner Production, 2018Co-Authors: Huiming Zhang, Lifang Xiong, Sanfeng ZhangAbstract:Abstract Since 2003, the evaluation system of government officials in China has undergone significant changes from relying on almost solely on traditional economic growth criteria to a broader set of indicators including environmental quality and per-capita disposable income of urban residents. Resource-exhausted cities face severe problems, such as environmental pollution and industrial restructuring, during this process of city Transformation. While addressing these problems, local officials, especially local top leaders, play a very important role, because their performance under this new evaluation system, to a certain extent, impacts the Transformation Efficiency of resource-exhausted cities. Based on this, we analyze the relationship between political incentives and city Transformation Efficiency by applying a panel dataset of 37 resource-exhausted cities from the 2004–2014. The findings reveal that: (1) Transformation Efficiency has been improved noticeably over the final two years of the sample period following 8 years of fluctuation without meaningful improvement. (2) Of the factors which impact the Transformation Efficiency of resource-exhausted cities, foreign direct investment, fiscal deficit and number of internet users produce only weak effects. This indicates that investment openness, fiscal surplus of local government, and infrastructure construction are not key variables in boosting urban Transformation. (3) The impact of political incentives on the Transformation Efficiency of resource-exhausted cities depends on whether the officials are municipal party secretaries or mayors. The results confirm that the existing authority structure in China has an effect on the Transformation of resource-exhausted cities and imply that, in contrast to the municipal party secretaries, the mayors take a subordinate position in the authority structure, thus failing to exert their effective role in the process of urban Transformation.
Pietro D. Spanu - One of the best experts on this subject based on the ideXlab platform.
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Yeast Transformation Efficiency is enhanced by TORC1‐ and eisosome‐dependent signaling
Wiley, 2019Co-Authors: Florian Kuemmel, Maria‐nefeli Skoufou‐papoutsaki, Pietro D. SpanuAbstract:Abstract Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning Transformation are still unclear. The addition of amino acids to the growth and Transformation medium increases Transformation Efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances Transformation via ubiquitin‐mediated endocytosis. We created mutants of the TORC1 pathway, alpha‐arrestins, and eisosome‐related genes. Our results demonstrate that the TORC1‐Npr1‐Art1/Rsp5 pathway regulates yeast Transformation. Based on our previous study, activation of this pathway results in up to a 200‐fold increase in Transformation Efficiency, or greater. Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on Transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimize gene therapy
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Yeast Transformation Efficiency is enhanced by TORC1- and eisosome-dependent signaling.
MicrobiologyOpen, 2018Co-Authors: Florian Kuemmel, Maria-nefeli Skoufou-papoutsaki, Pietro D. SpanuAbstract:Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning Transformation are still unclear. The addition of amino acids to the growth and Transformation medium increases Transformation Efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances Transformation via ubiquitin-mediated endocytosis. We created mutants of the TORC1 pathway, alpha-arrestins, and eisosome-related genes. Our results demonstrate that the TORC1-Npr1-Art1/Rsp5 pathway regulates yeast Transformation. Based on our previous study, activation of this pathway results in up to a 200-fold increase in Transformation Efficiency, or greater. Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on Transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimize gene therapy.
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Yeast Transformation Efficiency is enhanced by TORC1- and eisosome-dependent signalling
2018Co-Authors: Florian Kuemmel, Maria-nefeli Skoufou-papoutsaki, Pietro D. SpanuAbstract:Transformation of baker9s yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning Transformation are still unclear. Addition of amino acids to the growth and Transformation medium increases Transformation Efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances Transformation via ubiquitin-mediated endocytosis. We created mutants of the TORC1 pathway, α-arrestins, and eisosome-related genes. Our results demonstrate that the TORC1-Npr1-Art1/Rsp5 pathway regulates yeast Transformation. Based on our previous study, activation of this pathway results in a 13-fold increase in Transformation Efficiency, or greater. Additionally, we suggest DNA is taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on Transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimise gene therapy.
Jun Ren - One of the best experts on this subject based on the ideXlab platform.
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Identification of a cytosine methyltransferase that improves Transformation Efficiency in Methylomonas sp. DH-1
Biotechnology for Biofuels, 2020Co-Authors: Jun Ren, Hyang-mi Lee, Thi Duc ThaiAbstract:Background Industrial biofuels and other value-added products can be produced from metabolically engineered microorganisms. Methylomonas sp. DH-1 is a candidate platform for bioconversion that uses methane as a carbon source. Although several genetic engineering techniques have been developed to work with Methylomonas sp. DH-1, the genetic manipulation of plasmids remains difficult because of the restriction-modification (RM) system present in the bacteria. Therefore, the RM system in Methylomonas sp. DH-1 must be identified to improve the genetic engineering prospects of this microorganism. Results We identified a DNA methylation site, TGGCCA, and its corresponding cytosine methyltransferase for the first time in Methylomonas sp. DH-1 through whole-genome bisulfite sequencing. The methyltransferase was confirmed to methylate the fourth nucleotide of TGG C CA. In general, methylated plasmids exhibited better Transformation Efficiency under the protection of the RM system than non-methylated plasmids did. As expected, when we transformed Methylomonas sp. DH-1 with plasmid DNA harboring the psy gene, the metabolic flux towards carotenoid increased. The methyltransferase-treated plasmid exhibited an increase in Transformation Efficiency of 2.5 × 10^3 CFU/μg (124%). The introduced gene increased the production of carotenoid by 26%. In addition, the methyltransferase-treated plasmid harboring anti- psy sRNA gene exhibited an increase in Transformation Efficiency by 70% as well. The production of carotenoid was decreased by 40% when the psy gene was translationally repressed by anti- psy sRNA. Conclusions Plasmid DNA methylated by the discovered cytosine methyltransferase from Methylomonas sp. DH-1 had a higher Transformation Efficiency than non-treated plasmid DNA. The RM system identified in this study may facilitate the plasmid-based genetic manipulation of methanotrophs.
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Identification of a cytosine methyltransferase that improves Transformation Efficiency in Methylomonas sp. DH-1
2020Co-Authors: Jun Ren, Hyang-mi Lee, Thai Duc ThiAbstract:Abstract Background: Industrial biofuels and other value-added products can be produced from metabolically engineered microorganisms. Methylomonas sp. DH-1 is a candidate platform for bioconversion that uses methane as a carbon source. Although several genetic engineering techniques have been developed to work with Methylomonas sp. DH-1, the genetic manipulation of plasmids remains difficult because of the restriction-modification (RM) system present in the bacteria. Therefore, the RM system in Methylomonas sp. DH-1 must be identified to improve the genetic engineering prospects of this microorganism.Results: We identified a DNA methylation site, TGGCCA, and its corresponding cytosine methyltransferase for the first time in Methylomonas sp. DH-1 through whole-genome bisulfite sequencing. The methyltransferase was confirmed to methylate the fourth nucleotide of TGGC*CA. In general, methylated plasmids exhibited better Transformation Efficiency under the protection of the RM system than non-methylated plasmids did. As expected, when we transformed Methylomonas sp. DH-1 with plasmid DNA harboring the psy gene, the metabolic flux towards carotenoid increased. The methyltransferase-treated plasmid exhibited an increase in Transformation Efficiency of 3.9 × 103 CFU/μg (110 %). The introduced gene increased the production of carotenoid by 7.2 %.Conclusions: Plasmid DNA methylated by the discovered cytosine methyltransferase from Methylomonas sp. DH-1 had a higher Transformation Efficiency than non-treated plasmid DNA. The RM system identified in this study may facilitate the plasmid-based genetic manipulation of methanotrophs.