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Dibyajyoti Banerjee - One of the best experts on this subject based on the ideXlab platform.
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Development of a point of care testing tool to classify peritoneal effusion as exudate and Transudate.
Clinica Chimica Acta, 2011Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Subir Kumar Bandyopadhyay, Dibyajyoti BanerjeeAbstract:Abstract Background Recently we developed a bedside test to classify pleural effusion into exudate and Transudate but point of care classification of peritoneal effusion is yet not published. Methods We analyzed the Boyer's criteria parameters from bloodless peritoneal fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was used and 10 μl of 30% hydrogen peroxide was added, followed by inspection of the sample for the appearance of bubbles. Results All exudative ascitic fluids (n = 50) have shown the appearance of profuse bubbles within 1 min addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative ascitic fluids (n = 50) have not shown bubble formation within 1 min after the addition of hydrogen peroxide. The exudate does not show bubble formation if added with catalase inhibitors prior to the addition of hydrogen peroxide. Blood mixed Transudate have shown profuse bubble formation after the addition of hydrogen peroxide. Conclusion The hydrogen peroxide bubbling reaction has the potential to be developed as a point of care test to classify peritoneal fluid as exudate or Transudate.
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Development of a point of care testing tool to classify peritoneal effusion as exudate and Transudate.
Clinica chimica acta; international journal of clinical chemistry, 2011Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Subir Bandyopadhyay, Dibyajyoti BanerjeeAbstract:Recently we developed a bedside test to classify pleural effusion into exudate and Transudate but point of care classification of peritoneal effusion is yet not published. We analyzed the Boyer's criteria parameters from bloodless peritoneal fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was used and 10 μl of 30% hydrogen peroxide was added, followed by inspection of the sample for the appearance of bubbles. All exudative ascitic fluids (n=50) have shown the appearance of profuse bubbles within 1 min addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative ascitic fluids (n=50) have not shown bubble formation within 1 min after the addition of hydrogen peroxide. The exudate does not show bubble formation if added with catalase inhibitors prior to the addition of hydrogen peroxide. Blood mixed Transudate have shown profuse bubble formation after the addition of hydrogen peroxide. The hydrogen peroxide bubbling reaction has the potential to be developed as a point of care test to classify peritoneal fluid as exudate or Transudate. Copyright © 2011 Elsevier B.V. All rights reserved.
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A drop of hydrogen peroxide can differentiate exudative pleural effusion from Transudate--development of a bedside screening test.
Clinica chimica acta; international journal of clinical chemistry, 2009Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Soumya Bhattacharjee, Dibyajyoti BanerjeeAbstract:There is no bedside test to classify pleural fluid as exudate or Transudate. The aim of the present study is to develop such a test. We analyzed the Light's criteria parameters from bloodless pleural fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was taken and added with 10 microl of 30% hydrogen peroxide followed by inspection of the sample for appearance of bubbles. All exudative fluids (n=52) have shown appearance of profuse bubbles within 1 min of addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative fluids (n=32) have not shown bubble formation within 1 min after addition of hydrogen peroxide. The exudate does not show bubble formation if supplemented with catalase inhibitors. Blood mixed Transudate have shown profuse bubble formation after addition of hydrogen peroxide. In the case of blood uncontaminated pleural fluid, this newly developed protocol's sensitivity and specificity will be equivalent to Light's criteria probably with more advantage as by this procedure transport of the sample to the clinical laboratory is not required due to its inherent simplicity.
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A drop of hydrogen peroxide can differentiate exudative pleural effusion from Transudate--development of a bedside screening test.
Clinica Chimica Acta, 2009Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Soumya Bhattacharjee, Dibyajyoti BanerjeeAbstract:Abstract Background There is no bedside test to classify pleural fluid as exudate or Transudate. The aim of the present study is to develop such a test. Methods We analyzed the Light's criteria parameters from bloodless pleural fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was taken and added with 10 μl of 30% hydrogen peroxide followed by inspection of the sample for appearance of bubbles. Result All exudative fluids ( n = 52) have shown appearance of profuse bubbles within 1 min of addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative fluids ( n = 32) have not shown bubble formation within 1 min after addition of hydrogen peroxide. The exudate does not show bubble formation if supplemented with catalase inhibitors. Blood mixed Transudate have shown profuse bubble formation after addition of hydrogen peroxide. Conclusion In the case of blood uncontaminated pleural fluid, this newly developed protocol's sensitivity and specificity will be equivalent to Light's criteria probably with more advantage as by this procedure transport of the sample to the clinical laboratory is not required due to its inherent simplicity.
Oktay Imecik - One of the best experts on this subject based on the ideXlab platform.
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Diagnostic efficiency of pseudocholinesterase level in discrimination of Transudates-exudates
Tuberkuloz ve toraks, 2003Co-Authors: Faruk Ozer, Fikret Kanat, Abdülcelil Kalem, Oktay ImecikAbstract:In this study, 80 cases with pleural effusion hospitalized at the departments of Chest Diseases and Internal Medicine in the Medical School of Selcuk University, and 30 healthy people as control group were studied. Regarding to the diagnosis, pleural fluids were classified into two groups as follows; Transudates and exudates. Difference between the mean pleural pseudocholinesterase levels of Transudates and exudates was statistically significant (p< 0.001). Similar significance was also obtained in the mean pleural fluid/serum pseudocholinesterase ratios of the groups (p< 0.001). In determination of exudative fluids both sensitivity and specificity of the pleural fluid pseudocholinesterase level was 100%. Sensitivity and specificity of the pleural fluid/serum pseudocholinesterase ratio were 90 and 87%, respectively. We have concluded that pleural pseudocholinesterase level and pleural fluid/serum pseudocholinesterase ratio can be used as a parameter with high diagnostic efficiency in discrimination of pleural effusions as exudates and Transudates.
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Diagnostic value of uric acid to differentiate Transudates and exudates.
Clinical chemistry and laboratory medicine, 2000Co-Authors: Kürşat Uzun, Hüseyin Vural, Faruk Ozer, Oktay ImecikAbstract:Uric acid is known to be an end product of purine metabolism. Increases in uric acid may be found in clinical conditions associated with tissue hypoxia. We have investigated the value of uric acid to differentiate between a Transudate and exudate. In this study, we measured uric acid in the pleural fluid and the serum of 110 patients, 30 women and 80 men with a mean age of 49.5±19 years. Light's criteria were used to differentiate between a Transudate and exudate. Mean serum uric acid was 496.7±153.4 μmol/l in patients with Transudates and 291.3±143.1 μmol/l in patients with exudates. Mean pleural fluid uric acid was 487.7±165 μmol/l in patients with Transudates and 279.9±142.1 μmol/l in patients with exudates. These data showed that the levels of serum and pleural uric acid were higher in Transudates than exudates (p 0.05). The specificity and sensitivity of pleural uric acid for diagnosis of Transudate effusions were 73% and 80.6%, respectively. The specificity and sensitivity of pleural uric acid for diagnosis of Transudate effusions from exudates without malignancy were 71.8% and 91.7%, respectively. The sensitivity and specificity of pleural lactate dehydrogenase for diagnosis of exudates were 82% and 89%; the sensitivity and specificity of pleural fluid/serum lactate dehydrogenase were 85% and 89%; the sensitivity and specificity of pleural fluid/serum protein were 91% and 89%, respectively. Using all three of Light's criteria together, the sensitivity was 91% and its specificity was 94%. Our findings indicate that determination of uric acid in pleural fluid may be of diagnostic value in differential diagnosis of Transudates and exudates. The sensitivity of pleural uric acid measurement was higher for exudates without malignancy. However, Light's criteria remain the best means of separating Transudates from exudates.
John G Mchutchison - One of the best experts on this subject based on the ideXlab platform.
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the serum ascites albumin gradient is superior to the exudate Transudate concept in the differential diagnosis of ascites
Annals of Internal Medicine, 1992Co-Authors: Bruce A Runyon, Agnes A Montano, Evangelos A Akriviadis, Mainor R Antillon, Michelle A Irving, John G MchutchisonAbstract:Abstract ▪Objective:To compare the serum-ascites albumin gradient to the exudate-Transudate concept in the classification of ascites. ▪Design:Prospective collection of ascitic fluid data from patie...
Juan Custardoy - One of the best experts on this subject based on the ideXlab platform.
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Pleural fluid to serum cholinesterase ratio for the separation of Transudates and exudates.
Chest, 1996Co-Authors: Eduardo Garcia-pachon, Isabel Padilla-navas, Jose F. Sanchez, Benedicto Jimenez, Juan CustardoyAbstract:Study objective To evaluate the usefulness of two new parameters for separating pleural Transudates and exudates: pleural fluid cholinesterase level and pleural fluid to serum cholinesterase ratio, and to compare the results with the other well-established criteria. Design Prospective evaluation of the patients referred for diagnostic thoracentesis. Setting Pulmonary sections of a community hospital and a university hospital. Patients One hundred ninety-three consecutive patients. Forty were excluded for different reasons. Measurements The following criteria for separating the pleural effusions in Transudates and exudates were analyzed: Light's criteria, the pleural fluid cholesterol level, the pleural fluid to serum cholesterol ratio, the pleural fluid cholinesterase level, and the pleural fluid to serum cholinesterase ratio. Results One hundred fifty-three patients had conditions diagnosed. Thirty-five were classified as having Transudates and 118 as exudates. The percentage of effusions misclassified by each parameter was as follows: Light's criteria, 7.8%; pleural fluid cholesterol, 7.8%; pleural fluid to serum cholesterol ratio, 6.5%; pleural fluid cholinesterase, 8.5%; and pleural fluid to serum cholinesterase ratio, 1.3%. Conclusions The pleural fluid to serum cholinesterase ratio is the most accurate criterion for separating pleural Transudates and exudates. If further studies confirm our results, the cholinesterase ratio could be used as the first step in the diagnosis of pleural effusions.
Subendu Sarkar - One of the best experts on this subject based on the ideXlab platform.
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Development of a point of care testing tool to classify peritoneal effusion as exudate and Transudate.
Clinica Chimica Acta, 2011Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Subir Kumar Bandyopadhyay, Dibyajyoti BanerjeeAbstract:Abstract Background Recently we developed a bedside test to classify pleural effusion into exudate and Transudate but point of care classification of peritoneal effusion is yet not published. Methods We analyzed the Boyer's criteria parameters from bloodless peritoneal fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was used and 10 μl of 30% hydrogen peroxide was added, followed by inspection of the sample for the appearance of bubbles. Results All exudative ascitic fluids (n = 50) have shown the appearance of profuse bubbles within 1 min addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative ascitic fluids (n = 50) have not shown bubble formation within 1 min after the addition of hydrogen peroxide. The exudate does not show bubble formation if added with catalase inhibitors prior to the addition of hydrogen peroxide. Blood mixed Transudate have shown profuse bubble formation after the addition of hydrogen peroxide. Conclusion The hydrogen peroxide bubbling reaction has the potential to be developed as a point of care test to classify peritoneal fluid as exudate or Transudate.
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Development of a point of care testing tool to classify peritoneal effusion as exudate and Transudate.
Clinica chimica acta; international journal of clinical chemistry, 2011Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Subir Bandyopadhyay, Dibyajyoti BanerjeeAbstract:Recently we developed a bedside test to classify pleural effusion into exudate and Transudate but point of care classification of peritoneal effusion is yet not published. We analyzed the Boyer's criteria parameters from bloodless peritoneal fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was used and 10 μl of 30% hydrogen peroxide was added, followed by inspection of the sample for the appearance of bubbles. All exudative ascitic fluids (n=50) have shown the appearance of profuse bubbles within 1 min addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative ascitic fluids (n=50) have not shown bubble formation within 1 min after the addition of hydrogen peroxide. The exudate does not show bubble formation if added with catalase inhibitors prior to the addition of hydrogen peroxide. Blood mixed Transudate have shown profuse bubble formation after the addition of hydrogen peroxide. The hydrogen peroxide bubbling reaction has the potential to be developed as a point of care test to classify peritoneal fluid as exudate or Transudate. Copyright © 2011 Elsevier B.V. All rights reserved.
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A drop of hydrogen peroxide can differentiate exudative pleural effusion from Transudate--development of a bedside screening test.
Clinica chimica acta; international journal of clinical chemistry, 2009Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Soumya Bhattacharjee, Dibyajyoti BanerjeeAbstract:There is no bedside test to classify pleural fluid as exudate or Transudate. The aim of the present study is to develop such a test. We analyzed the Light's criteria parameters from bloodless pleural fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was taken and added with 10 microl of 30% hydrogen peroxide followed by inspection of the sample for appearance of bubbles. All exudative fluids (n=52) have shown appearance of profuse bubbles within 1 min of addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative fluids (n=32) have not shown bubble formation within 1 min after addition of hydrogen peroxide. The exudate does not show bubble formation if supplemented with catalase inhibitors. Blood mixed Transudate have shown profuse bubble formation after addition of hydrogen peroxide. In the case of blood uncontaminated pleural fluid, this newly developed protocol's sensitivity and specificity will be equivalent to Light's criteria probably with more advantage as by this procedure transport of the sample to the clinical laboratory is not required due to its inherent simplicity.
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A drop of hydrogen peroxide can differentiate exudative pleural effusion from Transudate--development of a bedside screening test.
Clinica Chimica Acta, 2009Co-Authors: Subendu Sarkar, Gorachand Bhattacharya, Soumya Bhattacharjee, Dibyajyoti BanerjeeAbstract:Abstract Background There is no bedside test to classify pleural fluid as exudate or Transudate. The aim of the present study is to develop such a test. Methods We analyzed the Light's criteria parameters from bloodless pleural fluid and classified the biofluid as exudate or Transudate and also estimated some parameters of oxidative stress in the biofluid by established spectrophotometric procedure. Two hundred microliters of sample was taken and added with 10 μl of 30% hydrogen peroxide followed by inspection of the sample for appearance of bubbles. Result All exudative fluids ( n = 52) have shown appearance of profuse bubbles within 1 min of addition of hydrogen peroxide along with significantly more catalase activity compared to Transudate. All transudative fluids ( n = 32) have not shown bubble formation within 1 min after addition of hydrogen peroxide. The exudate does not show bubble formation if supplemented with catalase inhibitors. Blood mixed Transudate have shown profuse bubble formation after addition of hydrogen peroxide. Conclusion In the case of blood uncontaminated pleural fluid, this newly developed protocol's sensitivity and specificity will be equivalent to Light's criteria probably with more advantage as by this procedure transport of the sample to the clinical laboratory is not required due to its inherent simplicity.
