The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Yifan Lyu - One of the best experts on this subject based on the ideXlab platform.
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engineering a 3d dna logic gate nanomachine for bispecific recognition and computing on target cell surfaces
Journal of the American Chemical Society, 2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics.
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Engineering a 3D DNA-Logic Gate Nanomachine for Bispecific Recognition and Computing on Target Cell Surfaces
2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics
Ruizi Peng - One of the best experts on this subject based on the ideXlab platform.
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engineering a 3d dna logic gate nanomachine for bispecific recognition and computing on target cell surfaces
Journal of the American Chemical Society, 2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics.
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Engineering a 3D DNA-Logic Gate Nanomachine for Bispecific Recognition and Computing on Target Cell Surfaces
2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics
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fluorescence resonance energy transfer based dna nanoPrism with a split aptamer for adenosine triphosphate sensing in living cells
Analytical Chemistry, 2017Co-Authors: Xiaofang Zheng, Ruizi Peng, Qiaoling Liu, Shuangyan Huan, Xi Jiang, Yaya Wang, Xiaobing ZhangAbstract:We have developed a DNA nanoprobe for adenosine triphosphate (ATP) sensing in living cells, based on the split aptamer and the DNA Triangular Prism (TP). In which nucleic acid aptamer was split into two fragments, the stem of the split aptamer was respectively labeled donor and acceptor fluorophores that underwent a fluorescence resonance energy transfer if two ATP molecules were bound as target molecule to the recognition module. Hence, ATP as a target induced the self-assembly of split aptamer fragments and thereby brought the dual fluorophores into close proximity for high fluorescence resonance energy transfer (FRET) efficiency. In the in vitro assay, an almost 5-fold increase in FA/FD signal was observed, the fluorescence emission ratio was found to be linear with the concentration of ATP in the range of 0.03–2 mM, and the nanoprobe was highly selective toward ATP. For the strong protecting capability to nucleic acids from enzymatic cleavage and the excellent biocompatibility of the TP, the DNA TP na...
Xiaobing Zhang - One of the best experts on this subject based on the ideXlab platform.
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engineering a 3d dna logic gate nanomachine for bispecific recognition and computing on target cell surfaces
Journal of the American Chemical Society, 2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics.
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Engineering a 3D DNA-Logic Gate Nanomachine for Bispecific Recognition and Computing on Target Cell Surfaces
2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics
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fluorescence resonance energy transfer based dna nanoPrism with a split aptamer for adenosine triphosphate sensing in living cells
Analytical Chemistry, 2017Co-Authors: Xiaofang Zheng, Ruizi Peng, Qiaoling Liu, Shuangyan Huan, Xi Jiang, Yaya Wang, Xiaobing ZhangAbstract:We have developed a DNA nanoprobe for adenosine triphosphate (ATP) sensing in living cells, based on the split aptamer and the DNA Triangular Prism (TP). In which nucleic acid aptamer was split into two fragments, the stem of the split aptamer was respectively labeled donor and acceptor fluorophores that underwent a fluorescence resonance energy transfer if two ATP molecules were bound as target molecule to the recognition module. Hence, ATP as a target induced the self-assembly of split aptamer fragments and thereby brought the dual fluorophores into close proximity for high fluorescence resonance energy transfer (FRET) efficiency. In the in vitro assay, an almost 5-fold increase in FA/FD signal was observed, the fluorescence emission ratio was found to be linear with the concentration of ATP in the range of 0.03–2 mM, and the nanoprobe was highly selective toward ATP. For the strong protecting capability to nucleic acids from enzymatic cleavage and the excellent biocompatibility of the TP, the DNA TP na...
Weihong Tan - One of the best experts on this subject based on the ideXlab platform.
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engineering a 3d dna logic gate nanomachine for bispecific recognition and computing on target cell surfaces
Journal of the American Chemical Society, 2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics.
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Engineering a 3D DNA-Logic Gate Nanomachine for Bispecific Recognition and Computing on Target Cell Surfaces
2018Co-Authors: Ruizi Peng, Xiaofang Zheng, Yifan Lyu, Xiaobing Zhang, Qiaoling Liu, Changjun You, Shuangyan Huan, Weihong TanAbstract:Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator “AND” returns a true value, it is followed by an “ON” signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate Triangular Prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics
H Abbassi - One of the best experts on this subject based on the ideXlab platform.
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numerical investigation of forced convection in a horizontal channel with a built in Triangular Prism
Journal of Heat Transfer-transactions of The Asme, 2002Co-Authors: H Abbassi, S Turki, Ben S NasrallahAbstract:Structure of laminar flow and heat transfer, in a two-dimensional horizontal channel differentially heated, with a builtin Triangular Prism is investigated from the numerical solutions of complete Navier–Stokes and energy equations. The numerical scheme is based on Control Volume Finite-Element Method with the SIMPLER algorithm for pressure-velocity coupling. Many standard test flows are successfully simulated. Results are obtained for Reynolds number ranging from 20 to 250 at Pr =0.71 with constant physical properties. The flow is especially studied in details for two Reynolds numbers, Re =30 as a sample of the symmetric flow, and Re =100 as a sample for the periodic flow. Results are presented to show how the presence of such bluff body affects the flow pattern and the heat transfer from the hot bottom plate to the flow in both cases, symmetric and periodic flows. 2001 Editions scientifiques et medicales Elsevier SAS laminar flow / periodic / obstacle / heat transfer / shedding / vortices / forced convection Resume—La convection forcee d’air dans un canal plan differentiellement chauffe et contenant un obstacle ayant la forme d’un Prisme triangulaire est etudiee numeriquement. Les equations de conservation sont resolues pour une geometrie bidimensionnelle par une methode de volumes finis a maillage non structure en conservant la vitesse et la pression comme variables dynamiques du probleme. Plusieurs tests de validations du code de calcul ont ete realises avec succes. Les resultats sont obtenus pour Reynolds allant de 20 a 250 pour Pr =0,71. L’ecoulement est particulierement etudie en details pour Re =30 et Re =100 comme etant deux echantillons respectifs de l’ecoulement symetrique et de l’ecoulement periodique. L’objectif de ce travail est l’etude de l’effet de l’obstacle sur la structure de l’ecoulement et sur les transferts thermiques. 2001 Editions scientifiques et medicales Elsevier SAS ecoulement laminaire / periodique / obstacle / transfert thermique / detachement / tourbillons / convection forcee
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channel flow past bluff body outlet boundary condition vortex shedding and effects of buoyancy
Computational Mechanics, 2002Co-Authors: H Abbassi, S Turki, Ben S NasrallahAbstract:Structure of laminar flow and heat transfer, in a two-dimensional horizontal plane channel differentially heated, with a built-in Triangular Prism is investigated from the numerical solutions of complete Navier–Stokes and energy equations. Results are obtained for Reynolds and Grashof numbers ranging respectively from 30 to 200 and from 0 to 1.5 × 104 at Pr=0.71. In forced convection, results are specially presented to show how the vortex shedding at downstream affects the upstream. Also, two correlations giving the Strouhal and the averaged Nusselt numbers as functions of the Reynolds number are proposed. In mixed convection, the superposition of Von Karman street and convective cells is discussed.
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numerical investigation of forced convection in a plane channel with a built in Triangular Prism
International Journal of Thermal Sciences, 2001Co-Authors: H Abbassi, S Turki, Sassi Ben NasrallahAbstract:Abstract Structure of laminar flow and heat transfer, in a two-dimensional horizontal channel differentially heated, with a built-in Triangular Prism is investigated from the numerical solutions of complete Navier–Stokes and energy equations. The numerical scheme is based on Control Volume Finite-Element Method with the SIMPLER algorithm for pressure-velocity coupling. Many standard test flows are successfully simulated. Results are obtained for Reynolds number ranging from 20 to 250 at Pr= 0.71 with constant physical properties. The flow is especially studied in details for two Reynolds numbers, Re= 30 as a sample of the symmetric flow, and Re= 100 as a sample for the periodic flow. Results are presented to show how the presence of such bluff body affects the flow pattern and the heat transfer from the hot bottom plate to the flow in both cases, symmetric and periodic flows.
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mixed convection in a plane channel with a built in Triangular Prism
Numerical Heat Transfer Part A-applications, 2001Co-Authors: H Abbassi, S Turki, Ben S NasrallahAbstract:The superposition of Von Karman street and convective cells in a horizontal plane channel containing a Triangular Prism and heated from below constitute the principal subject of this numerical investigation. The numerical scheme is based on the control volume finite element method (CVFEM) adapted to the standard staggered grid with the SIM PLER algorithm for pressure-velocity coupling and an Alternating Direction Implicit (ADI) scheme for the time integration. Many standard test flows are simulated successfully. Results are obtained for a Grashof number ranging from 0 to 1.5 10 4 at Pr 0.71 and Re 100 with constant physical properties. At the outlet of the computational domain a convective boundary condition (CBC) is used. Results are presented to show the effect of development of convective cells on the flow pattern and on the Strouhal number. Regarding the heat transfer rate, we focus on the effect of the presence of the Triangular Prism on the heat flux transferred from the hot wall to the flow.
