The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Mingshan Ji - One of the best experts on this subject based on the ideXlab platform.
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phytochemical analysis biochemical and mineral composition and gc ms profiling of methanolic extract of chinese arrowhead sagittaria trifolia l from northeast china
Molecules, 2019Co-Authors: Maqsood Ahmed, Mingshan Ji, Aatika Sikandar, Aafia Iram, Ansar Javeed, Jamil Shafi, Zeeshan Iqbal, Mazher Farid IqbalAbstract:Sagittaria trifolia is a medicinal foodstuff of China and East Asia belonging to the family Alismataceae. Samples of S. trifolia tubers were collected from Meihekow, Siping, Jilin, Harbin and Wuchang from Northeast China. The current study was aimed to evaluate the qualitative and quantitative analysis, antioxidant activity, biochemical analysis and chemical composition of different populations of S. trifolia. By using Folin–Ciocalteu, aluminium chloride colourimetric and 1,1-diphenyl-1-picrylhydrazyl (DPPH), total phenol and flavonoids content and antioxidant activity was analysed. Furthermore, chemical composition, biochemical analysis and mineral substances were also determined. The results showed the presence of flavonoids, phenols, saponins, tannins, glycosides and steroids except for alkaloids and terpenoids by qualitative analysis. Quantitative analysis revealed that highest total phenol, flavonoids content and antioxidant potential identified from Meihekow, i.e., 2.307 mg GAE/g, 12.263 mg QE/g and 77.373%, respectively. Gas chromatography-mass spectrometry results showed the presence of 40 chemical compounds corresponding to 99.44% of total extract that might be responsible for antioxidant properties. Mineral and biochemical analysis revealed the presence of calcium, magnesium, potassium, sodium, iron, copper, zinc and, carbohydrate, protein, fibre and fat contents, respectively. Interestingly, all S. trifolia populations collected from different locations possess similar composition. The dietary values, phytoconstituents, antioxidant activities and nutritional and curative chemical compounds of S. trifolia are beneficial for the nutritherapy of human beings.
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bensulfuron methyl resistant sagittaria trifolia l multiple resistance cross resistance and molecular basis of resistance to acetolactate synthase inhibiting herbicides
Archives of Biological Sciences, 2017Co-Authors: Danni Fu, Jamil Shafi, Bochui Zhao, Xiuwei Li, Mingshan JiAbstract:Acetolactate synthase (ALS)-inhibiting herbicides play an important role in controlling broad-leaved weeds. Populations of Sagittaria trifolia L. showed resistance to ALS-inhibiting sulfonylurea herbicides (e.g. bensulfuron-methyl) in paddy fields in the northeast of China. In our study, whole-plant bioassays were performed on eight suspected resistant S. trifolia populations that showed high levels of resistance to bensulfuron-methyl, with resistance indices from 31.06 to 120.35. The results of ALS-activity assays were consistent with the observed whole-plant dose-response data. This confirmed that resistant populations displayed significantly higher ALS activity than the sensitive population due to prevention of normal enzyme-herbicide interaction. The mutations Pro-197-Ser, Pro-197-His, Pro-197-Thr and Pro-197-Leu were identified in the ALS gene of resistant populations. Pro-197-His and Pro-197-Thr mutations conferring resistance to bensulfuron-methyl are reported for the first time in S. trifolia . All resistant populations were resistant to sulfonylurea (SU) herbicides, but not to imidazolinone (IMI) herbicides. HLJ-5 and JL-3 populations were resistant to bispyribac-sodium of the pyrimidinyl-thiobenozoate (PTB) class of ALS herbicides, JL-2 to penoxsulam of triazolopyrimidine (TP) class and JL-1 to pyribenzoxim, also of PTB class. The eight S. trifolia populations were susceptible to other herbicide modes of action tested. https://doi.org/10.2298/ABS170210010F Received: February 10, 2017; Revised: March 6, 2017; Accepted: March 9, 2017; Published online: March 20, 2017 How to cite this article: Fu D, Shafi J, Zhao B, Li X, Zhu H, Wei S, Ji M. Bensulfuron-methyl resistant Sagittaria trifolia L.: Multiple resistance, cross-resistance and molecular basis of resistance to acetolactate synthase-inhibiting herbicides. Arch Biol Sci. 2017;69(4):649-58.
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non target site resistance to als inhibiting herbicides in a sagittaria trifolia l population
Pesticide Biochemistry and Physiology, 2017Co-Authors: Bochui Zhao, Jamil Shafi, Pingsheng Li, Danni Fu, Yang Yu, Chengtian Huang, Mingshan JiAbstract:Sagittaria trifolia L. is one of the most competitive weeds in rice fields in northeastern China. The continuous use of acetolactate synthase (ALS)-inhibitors has led to the evolution of herbicide resistant S. trifolia. A subpopulation BC1, which was derived from the L1 population, was analyzed using DNA sequencing and ALS enzyme activity assays and levels of resistance to five ALS-inhibiting herbicides was determined. DNA sequencing and ALS enzyme assays revealed no amino acid substitutions and no significant differences in enzyme sensitivity between susceptible and resistant populations. Whole-plant dose-response experiments showed that the BC1 population exhibited different levels of resistance (resistance ratios ranging from 2.14 to 51.53) to five ALS herbicides, and the addition of malathion (P450 inhibitor) to bensulfuron-methyl, penoxsulam and bispyribac-sodium strongly reduced the dry weight accumulation of the BC1 population compared with the effects of the three herbicides alone. The results of the present study demonstrated that the BC1 population has evolved non-target-site resistance to ALS-inhibiting herbicides.
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target site resistance to bensulfuron methyl in sagittaria trifolia l populations
Pesticide Biochemistry and Physiology, 2015Co-Authors: Pingsheng Li, Mingshan Ji, Qin Dong, Haining WangAbstract:Abstract Sagittaria trifolia L. is one of the most serious weeds in paddy fields in northeast of China and cannot be controlled effectively by bensulfuron-methyl in recent years. In this study, two suspected resistant S. trifolia populations (R1 and R2) were collected in Liaoning province of China. Whole-plant dose–response studies showed that R1 and R2 were highly resistant to bensulfuron-methyl, with the GR 50 R/S ratios of 76.99 and 49.94 respectively. In vitro acetolactate synthase (ALS) assays revealed that resistance was due to reduced sensitivity of the ALS to bensulfuron-methyl inhibition, with I 50 R/S ratios of 81.86 and 67.48 for R1 and R2, respectively. Total ALS activity was similar for the S and R2 populations, whereas the R1 population displayed significantly higher ALS activity than did the S population. The mutations Pro-197-Leu and Pro-197-Ser were identified in the ALS gene of the R1 and R2 populations, respectively. This is the first report examining bensulfuron-resistant S. trifolia in Liaoning province, China. The Pro197 mutation is likely responsible for resistance to bensulfuron-methyl in S. trifolia populations.
Jamil Shafi - One of the best experts on this subject based on the ideXlab platform.
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phytochemical analysis biochemical and mineral composition and gc ms profiling of methanolic extract of chinese arrowhead sagittaria trifolia l from northeast china
Molecules, 2019Co-Authors: Maqsood Ahmed, Mingshan Ji, Aatika Sikandar, Aafia Iram, Ansar Javeed, Jamil Shafi, Zeeshan Iqbal, Mazher Farid IqbalAbstract:Sagittaria trifolia is a medicinal foodstuff of China and East Asia belonging to the family Alismataceae. Samples of S. trifolia tubers were collected from Meihekow, Siping, Jilin, Harbin and Wuchang from Northeast China. The current study was aimed to evaluate the qualitative and quantitative analysis, antioxidant activity, biochemical analysis and chemical composition of different populations of S. trifolia. By using Folin–Ciocalteu, aluminium chloride colourimetric and 1,1-diphenyl-1-picrylhydrazyl (DPPH), total phenol and flavonoids content and antioxidant activity was analysed. Furthermore, chemical composition, biochemical analysis and mineral substances were also determined. The results showed the presence of flavonoids, phenols, saponins, tannins, glycosides and steroids except for alkaloids and terpenoids by qualitative analysis. Quantitative analysis revealed that highest total phenol, flavonoids content and antioxidant potential identified from Meihekow, i.e., 2.307 mg GAE/g, 12.263 mg QE/g and 77.373%, respectively. Gas chromatography-mass spectrometry results showed the presence of 40 chemical compounds corresponding to 99.44% of total extract that might be responsible for antioxidant properties. Mineral and biochemical analysis revealed the presence of calcium, magnesium, potassium, sodium, iron, copper, zinc and, carbohydrate, protein, fibre and fat contents, respectively. Interestingly, all S. trifolia populations collected from different locations possess similar composition. The dietary values, phytoconstituents, antioxidant activities and nutritional and curative chemical compounds of S. trifolia are beneficial for the nutritherapy of human beings.
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bensulfuron methyl resistant sagittaria trifolia l multiple resistance cross resistance and molecular basis of resistance to acetolactate synthase inhibiting herbicides
Archives of Biological Sciences, 2017Co-Authors: Danni Fu, Jamil Shafi, Bochui Zhao, Xiuwei Li, Mingshan JiAbstract:Acetolactate synthase (ALS)-inhibiting herbicides play an important role in controlling broad-leaved weeds. Populations of Sagittaria trifolia L. showed resistance to ALS-inhibiting sulfonylurea herbicides (e.g. bensulfuron-methyl) in paddy fields in the northeast of China. In our study, whole-plant bioassays were performed on eight suspected resistant S. trifolia populations that showed high levels of resistance to bensulfuron-methyl, with resistance indices from 31.06 to 120.35. The results of ALS-activity assays were consistent with the observed whole-plant dose-response data. This confirmed that resistant populations displayed significantly higher ALS activity than the sensitive population due to prevention of normal enzyme-herbicide interaction. The mutations Pro-197-Ser, Pro-197-His, Pro-197-Thr and Pro-197-Leu were identified in the ALS gene of resistant populations. Pro-197-His and Pro-197-Thr mutations conferring resistance to bensulfuron-methyl are reported for the first time in S. trifolia . All resistant populations were resistant to sulfonylurea (SU) herbicides, but not to imidazolinone (IMI) herbicides. HLJ-5 and JL-3 populations were resistant to bispyribac-sodium of the pyrimidinyl-thiobenozoate (PTB) class of ALS herbicides, JL-2 to penoxsulam of triazolopyrimidine (TP) class and JL-1 to pyribenzoxim, also of PTB class. The eight S. trifolia populations were susceptible to other herbicide modes of action tested. https://doi.org/10.2298/ABS170210010F Received: February 10, 2017; Revised: March 6, 2017; Accepted: March 9, 2017; Published online: March 20, 2017 How to cite this article: Fu D, Shafi J, Zhao B, Li X, Zhu H, Wei S, Ji M. Bensulfuron-methyl resistant Sagittaria trifolia L.: Multiple resistance, cross-resistance and molecular basis of resistance to acetolactate synthase-inhibiting herbicides. Arch Biol Sci. 2017;69(4):649-58.
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non target site resistance to als inhibiting herbicides in a sagittaria trifolia l population
Pesticide Biochemistry and Physiology, 2017Co-Authors: Bochui Zhao, Jamil Shafi, Pingsheng Li, Danni Fu, Yang Yu, Chengtian Huang, Mingshan JiAbstract:Sagittaria trifolia L. is one of the most competitive weeds in rice fields in northeastern China. The continuous use of acetolactate synthase (ALS)-inhibitors has led to the evolution of herbicide resistant S. trifolia. A subpopulation BC1, which was derived from the L1 population, was analyzed using DNA sequencing and ALS enzyme activity assays and levels of resistance to five ALS-inhibiting herbicides was determined. DNA sequencing and ALS enzyme assays revealed no amino acid substitutions and no significant differences in enzyme sensitivity between susceptible and resistant populations. Whole-plant dose-response experiments showed that the BC1 population exhibited different levels of resistance (resistance ratios ranging from 2.14 to 51.53) to five ALS herbicides, and the addition of malathion (P450 inhibitor) to bensulfuron-methyl, penoxsulam and bispyribac-sodium strongly reduced the dry weight accumulation of the BC1 population compared with the effects of the three herbicides alone. The results of the present study demonstrated that the BC1 population has evolved non-target-site resistance to ALS-inhibiting herbicides.
Bochui Zhao - One of the best experts on this subject based on the ideXlab platform.
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bensulfuron methyl resistant sagittaria trifolia l multiple resistance cross resistance and molecular basis of resistance to acetolactate synthase inhibiting herbicides
Archives of Biological Sciences, 2017Co-Authors: Danni Fu, Jamil Shafi, Bochui Zhao, Xiuwei Li, Mingshan JiAbstract:Acetolactate synthase (ALS)-inhibiting herbicides play an important role in controlling broad-leaved weeds. Populations of Sagittaria trifolia L. showed resistance to ALS-inhibiting sulfonylurea herbicides (e.g. bensulfuron-methyl) in paddy fields in the northeast of China. In our study, whole-plant bioassays were performed on eight suspected resistant S. trifolia populations that showed high levels of resistance to bensulfuron-methyl, with resistance indices from 31.06 to 120.35. The results of ALS-activity assays were consistent with the observed whole-plant dose-response data. This confirmed that resistant populations displayed significantly higher ALS activity than the sensitive population due to prevention of normal enzyme-herbicide interaction. The mutations Pro-197-Ser, Pro-197-His, Pro-197-Thr and Pro-197-Leu were identified in the ALS gene of resistant populations. Pro-197-His and Pro-197-Thr mutations conferring resistance to bensulfuron-methyl are reported for the first time in S. trifolia . All resistant populations were resistant to sulfonylurea (SU) herbicides, but not to imidazolinone (IMI) herbicides. HLJ-5 and JL-3 populations were resistant to bispyribac-sodium of the pyrimidinyl-thiobenozoate (PTB) class of ALS herbicides, JL-2 to penoxsulam of triazolopyrimidine (TP) class and JL-1 to pyribenzoxim, also of PTB class. The eight S. trifolia populations were susceptible to other herbicide modes of action tested. https://doi.org/10.2298/ABS170210010F Received: February 10, 2017; Revised: March 6, 2017; Accepted: March 9, 2017; Published online: March 20, 2017 How to cite this article: Fu D, Shafi J, Zhao B, Li X, Zhu H, Wei S, Ji M. Bensulfuron-methyl resistant Sagittaria trifolia L.: Multiple resistance, cross-resistance and molecular basis of resistance to acetolactate synthase-inhibiting herbicides. Arch Biol Sci. 2017;69(4):649-58.
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non target site resistance to als inhibiting herbicides in a sagittaria trifolia l population
Pesticide Biochemistry and Physiology, 2017Co-Authors: Bochui Zhao, Jamil Shafi, Pingsheng Li, Danni Fu, Yang Yu, Chengtian Huang, Mingshan JiAbstract:Sagittaria trifolia L. is one of the most competitive weeds in rice fields in northeastern China. The continuous use of acetolactate synthase (ALS)-inhibitors has led to the evolution of herbicide resistant S. trifolia. A subpopulation BC1, which was derived from the L1 population, was analyzed using DNA sequencing and ALS enzyme activity assays and levels of resistance to five ALS-inhibiting herbicides was determined. DNA sequencing and ALS enzyme assays revealed no amino acid substitutions and no significant differences in enzyme sensitivity between susceptible and resistant populations. Whole-plant dose-response experiments showed that the BC1 population exhibited different levels of resistance (resistance ratios ranging from 2.14 to 51.53) to five ALS herbicides, and the addition of malathion (P450 inhibitor) to bensulfuron-methyl, penoxsulam and bispyribac-sodium strongly reduced the dry weight accumulation of the BC1 population compared with the effects of the three herbicides alone. The results of the present study demonstrated that the BC1 population has evolved non-target-site resistance to ALS-inhibiting herbicides.
Pingsheng Li - One of the best experts on this subject based on the ideXlab platform.
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non target site resistance to als inhibiting herbicides in a sagittaria trifolia l population
Pesticide Biochemistry and Physiology, 2017Co-Authors: Bochui Zhao, Jamil Shafi, Pingsheng Li, Danni Fu, Yang Yu, Chengtian Huang, Mingshan JiAbstract:Sagittaria trifolia L. is one of the most competitive weeds in rice fields in northeastern China. The continuous use of acetolactate synthase (ALS)-inhibitors has led to the evolution of herbicide resistant S. trifolia. A subpopulation BC1, which was derived from the L1 population, was analyzed using DNA sequencing and ALS enzyme activity assays and levels of resistance to five ALS-inhibiting herbicides was determined. DNA sequencing and ALS enzyme assays revealed no amino acid substitutions and no significant differences in enzyme sensitivity between susceptible and resistant populations. Whole-plant dose-response experiments showed that the BC1 population exhibited different levels of resistance (resistance ratios ranging from 2.14 to 51.53) to five ALS herbicides, and the addition of malathion (P450 inhibitor) to bensulfuron-methyl, penoxsulam and bispyribac-sodium strongly reduced the dry weight accumulation of the BC1 population compared with the effects of the three herbicides alone. The results of the present study demonstrated that the BC1 population has evolved non-target-site resistance to ALS-inhibiting herbicides.
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target site resistance to bensulfuron methyl in sagittaria trifolia l populations
Pesticide Biochemistry and Physiology, 2015Co-Authors: Pingsheng Li, Mingshan Ji, Qin Dong, Haining WangAbstract:Abstract Sagittaria trifolia L. is one of the most serious weeds in paddy fields in northeast of China and cannot be controlled effectively by bensulfuron-methyl in recent years. In this study, two suspected resistant S. trifolia populations (R1 and R2) were collected in Liaoning province of China. Whole-plant dose–response studies showed that R1 and R2 were highly resistant to bensulfuron-methyl, with the GR 50 R/S ratios of 76.99 and 49.94 respectively. In vitro acetolactate synthase (ALS) assays revealed that resistance was due to reduced sensitivity of the ALS to bensulfuron-methyl inhibition, with I 50 R/S ratios of 81.86 and 67.48 for R1 and R2, respectively. Total ALS activity was similar for the S and R2 populations, whereas the R1 population displayed significantly higher ALS activity than did the S population. The mutations Pro-197-Leu and Pro-197-Ser were identified in the ALS gene of the R1 and R2 populations, respectively. This is the first report examining bensulfuron-resistant S. trifolia in Liaoning province, China. The Pro197 mutation is likely responsible for resistance to bensulfuron-methyl in S. trifolia populations.
Young Ryun Chung - One of the best experts on this subject based on the ideXlab platform.
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potential of an indigenous fungus plectosporium tabacinum as a mycoherbicide for control of arrowhead sagittaria trifolia
Plant Disease, 1998Co-Authors: Young Ryun ChungAbstract:ABSTRACT An isolate of the indigenous fungus Plectosporium tabacinum was isolated from arrowhead (Sagittaria trifolia) in Yusung, Korea in 1990 and evaluated in laboratory and growth chamber tests as a potential mycoherbicide. The fungus grew comparatively slowly on potato dextrose agar and corn meal agar, attaining a diameter of 65 mm after 12 days at 25°C. Conidia were mass-produced in shake-cultures or in a fermentor using potato dextrose broth containing yeast extract (0.5%, wt/vol) at 25°C. When arrowhead seedlings at the 2- to 3-leaf stage were inoculated with conidial suspensions (2 × 107 conidia/ml) and incubated in a dew chamber for 18 h at 25°C, the plants developed small, brown spots on the leaves and petioles in 2 days, and were blighted completely within 7 days after inoculation. This effect was consistent on arrowhead plants from the 2- to 5-leaf stage. Another arrowhead species, S. pygmaea, was as susceptible as S. trifolia to the pathogen. Several crops, including rice, barley, and wheat a...
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Potential of an indigenous fungus, Plectosporium tabacinum, as a mycoherbicide for control of arrowhead (sagittaria trifolia)
Plant disease, 1998Co-Authors: Young Ryun Chung, Suk Jin Koo, Heung Tae Kim, Kwang Yun ChoAbstract:An isolate of the indigenous fungus Plectosporium tabacinum was isolated from arrowhead (Sagittaria trifolia) in Yusung, Korea in 1990 and evaluated in laboratory and growth chamber tests as a potential mycoherbicide. The fungus grew comparatively slowly on potato dextrose agar and corn meal agar, attaining a diameter of 65 mm after 12 days at 25°C. Conidia were mass-produced in shake-cultures or in a fermentor using potato dextrose broth containing yeast extract (0.5%, wt/vol) at 25°C. When arrowhead seedlings at the 2- to 3-leaf stage were inoculated with conidial suspensions (2 × 107 conidia/ml) and incubated in a dew chamber for 18 h at 25°C, the plants developed small, brown spots on the leaves and petioles in 2 days, and were blighted completely within 7 days after inoculation. This effect was consistent on arrowhead plants from the 2- to 5-leaf stage. Another arrowhead species, S. pygmaea, was as susceptible as S. trifolia to the pathogen. Several crops, including rice, barley, and wheat and 34 other common weed species, were immune. In small-scale field tests in paddy fields during the summers of 1992 and 1993, a mean reduction of 71.3% in the number of arrowhead plants was observed following a foliar spray of a conidial suspension (107 conidia/ml). These results indicate that P. tabacinum has potential as a selective mycoherbicide for arrowhead control.
