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Robert W. Lichtwardt - One of the best experts on this subject based on the ideXlab platform.
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a parataeniella Trichomycetes ec crinales in an isopod
2016Co-Authors: Robert W. Lichtwardt, Alice Wheichu ChenAbstract:Infected pillbugs were discovered in a terrarium containing passalid beetles [Popilius disjunctus (Ill.)].2 The isopods probably were introduced with rotting wood and moldy leaves obtained from a woods about 3 miles northwest of Lawrence, in Douglas County, Section 21. Subsequent collections of Armadillidium from this woods confirmed their infection. A few pillbugs of the original colony maintained the endocommensal fungus in their guts after almost a year in culture. Most of the preparations were made by tearing out the hindgut of the living pillbugs with fine forceps, cutting open the gut, soaking it in dilute lactophenol (approximately 1 drop lactophenol per ml of water) to loosen the chitinous lining from the epithelial tissue, and finally mounting the lining with all attached thalli in lactophenol containing cotton blue.
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smittium spp Trichomycetes
2016Co-Authors: Abdulaziz M Elbuni, Robert W. LichtwardtAbstract:SUMMARY Harvested asexual spores (trichospores) of Smittium culisetae, S. culicis, S. simulii, and S. mucronatum were used in this study. Only spores of the first three species were able to germinate in vitro, with S. culisetae demonstrating the highest percentage of germination. Germination may be accomplished either by a rapid extension of the inner spore wall in a process that (under the light microscope) resembles germ tube formation, or by rupturing the outer trichospore wall and the total emergence of the inner spore wall and protoplast. Spores germinated better in nonshaken tryptone-glucose-vitamin (TGv) medium than in shaken medium. Smzittium culisetae spores appear to have an inhibitor that reduces the percentage of germination under crowded conditions. Germination in S. culisetae occurred over a relatively wide range of temperatures and pH values, with maxima near 24-30 C and pH 6.0-7.8. Spores of S. culisetae survived storage in liquid nitrogen, but lost their viability at -15 C and when lyophilized. Features of trichospores that make the Harpellales especially adapted to surviving in the gut of immature aquatic insects have been outlined in a previous article (El-Buni and Lichtwardt, 1976) which dealt primarily with the physiology of spore formation. The emphasis in the present study is on factors affecting germination in cultured Smittium species. Germination, as we use the term with trichospores, involves the initial stages of growth and development of the inner spore with its distinct wall, within the outer trichospore wall, a process that differs morphologically from typical germination in other fungi. Ultrastructural interpretations of trichospore development and germination are the subject of a forthcoming paper by Moss and Lichtwardt and will not be dis
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costa rican gut fungi Trichomycetes infeting lotic insect larvae
Revista De Biologia Tropical, 2015Co-Authors: Robert W. LichtwardtAbstract:Trichomycetes infecting freshwater Ephemeroptera and Diptera (Siinuliidae, Chironomidae) in various life zones oC Costa Rica were studied. Insect populations in 36 streams were sampled, a few repeatedly, at four different . perlods of time over seven years. Eleven new species of Harpellales are reported, plus six species known to occur also in n ontropical parts oi the world. Included are new species of Harpella and Pennella , a new monotypic genus ( Graminelloides) , and four new species of the previously monotypic genera Spartiella and Genistellospora . Seven new Smittium species, including three that are not named at this time, were cultured axenically. A new Amoebidium (Amoebidiales) was found in a stream polluted with organic matter. In the same polluted stream more species of dipteran larvae and a greater number of gut fungi were present than in stretclies of unpolluted water upstream. The overall diversity of Harpellales and their aquatic insect hosts in most Costa Rican streams appeared to be lower than what the author has found in many lotic habitats in more northem and southem regions of the world, and thus this order of fungi may be an exception to the 'latitudinal species diversity gradient' concept as it applies to many other tropical organisms. A key to all 23 Tric homycetes now known to be present in Costa Rica is provided
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Trichomycete gut fungi from tropical regions of the world
Biodiversity and Conservation, 2012Co-Authors: Robert W. LichtwardtAbstract:Fifty-nine species of gut fungi in the orders Harpellales and Asellariales, Class Trichomycetes, have been collected in eight tropical regions of the world, some species occurring in more than one geographic region. Regarding the Harpellales, the rather low number of taxa, compared to reports in more temperate localities, is due primarily to relatively few collections in the tropics, as well as the usually warmer waters found in tropical regions that often have a lower species richness of potential immature insect hosts. Asellariales in freshwater, marine, and terrestrial habitats, likewise, have been seldom inventoried in the tropics. Nonetheless, it is clear that the tropics are fertile grounds for discovering new genera and species, and future investigations will undoubtedly reveal many new taxa that will lead to a better understanding of the evolution and biogeography of Trichomycetes.
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Dacryodiomyces, a new genus of Harpellales in Chironomidae larvae
Mycologia, 2011Co-Authors: Robert W. LichtwardtAbstract:A new genus of gut fungus in the Harpellales (Trichomycetes) with the type species Dacryodiomyces oklahomensis, found in Chironomidae larvae, is described from northeastern Oklahoma, USA. The most distinguishing characters are the elongate-ovoid zygospores attached to their zygosporophore at one end (Type IV), together with trichospores bearing a collar and a single appendage.
Marvin C Williams - One of the best experts on this subject based on the ideXlab platform.
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symbiotic harpellales Trichomycetes in tasmanian aquatic insects
Mycologia, 2005Co-Authors: Leonard C. Ferrington, Robert W. Lichtwardt, Barbara Hayford, Marvin C WilliamsAbstract:Surveys for symbiotic fungi in the guts of aquatic insect larvae (Trichomycetes: Harpellales) in Tasmania, Australia, resulted in the discovery of four new species: two in Gripopterygidae (Plecoptera) nymphs, Plecopteromyces leptoperlarum and P. trinoto- perlarum, and two associated with Diptera larvae, Smittium magnosporum in Thaumaleidae and Stachy- lina dolichospora in Chironomidae. Previously de- scribed species of Harpellales from other localities are reported and new host records summarized. A key to all Tasmanian species of Harpellales is provid- ed.
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a new monotypic fungal genus allantomyces and a new species of legeriomyces Trichomycetes harpellales in the hindgut of a western australian mayfly nymph tasmanocoenis sp
Botany, 1993Co-Authors: Marvin C Williams, Robert W. LichtwardtAbstract:Allantomyces caenidarum sp.nov., a trichomycete fungus that lives in the hindgut of Tasmanocoenis sp. (Caenidae) mayfly nymphs in southwestern Western Australian streams, is described. In addition to normal trichospores and zygospores that serve to reinfest other nymphs, A. caenidarum also produces what may be modified trichospores that serve to increase gut infestation endogenously. The same species of mayfly also harbored a new species, Legeriomyces raus, which belongs to a genus previously known only from North America and Europe. Key words: Caenidae, Allantomyces, Australia, Tasmanocoenis, Trichomycetes, Legeriomyces.
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furculomyces a new homothallic genus of harpellales Trichomycetes from australian midge larvae
Botany, 1992Co-Authors: Robert W. Lichtwardt, Marvin C WilliamsAbstract:A new genus, Furculomyces, is established for two Australian species of trichomycete gut fungi that live in the hindguts of aquatic Chironomidae (Diptera) larvae. The new genus is based primarily on the sexual apparatus consisting of a swollen pair of homothallic conjugants that resemble a wishbone, from which develops a slightly bent biconical zygospore. The type species, Furculomyces boomerangus comb, no v. (basionym: Smittium boomerangum) has been found in Victorian and Tasmanian midges, whereas the new species, Furculomyces westraliensis, is from a midge species in southern Western Australia. These species support other evidence that native Australian gut fungi have a provincial distribution. Key words: Australian, Chironomidae, fungi, Furculomyces, Harpellales, Trichomycetes.
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Smittium bullatum from a New Zealand midge larva and new records of other trichomycete gut fungi
Botany, 1992Co-Authors: Robert W. Lichtwardt, Marvin C WilliamsAbstract:An apparently uncommon gut fungus, Smittium bullatum (Trichomycetes, Harpellales), is described from Chironomidae (Orthocladiinae) larvae living in South Island, New Zealand, streams. Records of other Harpellales in South Island aquatic insects are reported, including the presence of zygospores of the New Zealand species Pennella asymmetrica from Simuliidae larvae. The richness of some harpellid gut fungi in New Zealand is compared with that in Australia and other parts of the world, and the biogeography of Smittium is discussed. Key words: biogeography, Chironomidae, Diptera, New Zealand, Smittium, Trichomycetes.
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Tasmanian Trichomycete gut fungi in aquatic insect larvae.
Mycologia, 1992Co-Authors: Robert W. Lichtwardt, Marvin C WilliamsAbstract:A fourth species of the Australasian genus Austrosmittium, A. biforme (Trichomycetes, Harpellales), has been found. This gut fungus from Chironomidae larvae collected in a Tasmanian stream has the unusual feature of producing two distinctly different sizes of trichospores. Two new Tasmanian species of the more common genus Smittium, S. compactum and S. fastigatum, are described from lotic chironomid larvae. A new species of Harpellales from Tasmanian mayfly nymphs is also described and illustrated, but not named; it may represent a new genus that has similarities to Bojamyces repens recently described from the USA. New records of other Tasmanian Trichomycetes from aquatic insects are provided.
Merlin M White - One of the best experts on this subject based on the ideXlab platform.
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new species and geographical distribution of Trichomycetes gut fungi in a novel host caddisfly trichoptera
Botany, 2019Co-Authors: D B Strongman, Merlin M WhiteAbstract:Trichomycetes are fungi and protists living in the guts of arthropods. Herbivorous insects in freshwater habitats commonly house these microbes, and there are over 300 species described. One very c...
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resolving relationships at the animal fungal divergence a molecular phylogenetic study of the protist Trichomycetes ichthyosporea eccrinida
Molecular Phylogenetics and Evolution, 2017Co-Authors: Nicole Reynolds, Matias J Cafaro, Matthew E Smith, Eric D Tretter, Justin Gause, Dustin Heeney, James F Smith, Stephen J Novak, William A Bourland, Merlin M WhiteAbstract:Abstract Trichomycetes is a group of microorganisms that was considered a class of fungi comprising four orders of commensal, gut-dwelling endosymbionts obligately associated with arthropods. Since molecular phylogenies revealed two of those orders (Amoebidiales and Eccrinales = “protist trichos”) to be closely related to members of the protist class Ichthyosporea (=Mesomycetozoea), Trichomycetes have been considered an ecological association of both early-diverging fungi and protists. Understanding of the taxonomy, evolution, and diversity of the protist trichos is lacking largely due to the difficulties inherent in species collection that have contributed to undersampling and understudy. The most recent classification divides the protist trichos between two families, Amoebidiidae and Eccrinidae (suborder Trichomycina, order Eccrinida). However, there is no comprehensive molecular phylogeny available for this group and major questions about the systematics of protist trichos remain unanswered. Therefore, we generated 18S and 28S rDNA sequences for 106 protist tricho samples and combined them with publicly available Eccrinida sequences for phylogenetic analyses. We also sequenced a conserved protein-coding gene (heat-shock 70 protein) to obtain a multigene data set. We conducted ancestral state reconstruction (ASR) and Bayesian tip-association significance test (BaTS) analyses by mapping six morphological and ecological characters onto the resulting phylogenetic trees. Our results demonstrate: (1) several ecological and morphological character states (habitat, host type, host stage at time of infestation, location within host, spore production, and growth form) are significantly correlated with the phylogeny, and (2) two additional protist tricho families should be incorporated into the taxonomy to reflect phylogenetic relationships. Our data suggest that an integrated strategy that combines morphological, ecological, and molecular characters is needed to further resolve and clarify the systematics of the Eccrinida.
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new species of smittium and stachylina and other Trichomycetes in larval diptera from streams in nova scotia canada 1this paper is dedicated to jo ann frost one of the first students of these fungi in nova scotia and to the numerous other undergradua
Botany, 2012Co-Authors: Merlin M White, D B StrongmanAbstract:The guts of non-predaceous invertebrates in aquatic and moist terrestrial habitats are often colonized by an ecological group of microorganisms called Trichomycetes. Taxonomically, these endobionts are currently a diverse, polyphyletic assemblage including both zygomycetous fungi as well as protistan species. Trichomycetes are worldwide in distribution and are from varied habitats, but the species inventory of gut fungi from hosts in Canada is far from complete. We summarize the findings from our earliest surveys (from 1997 to 2005) and collections of candidate dipteran hosts in Nova Scotia. Nine new species of gut fungi are added to the inventory list, including the following seven Smittium spp.: Smittium aggregatum, Smittium gronthidium, Smittium papillum, Smittium pavocaudatum, Smittium radiculans, Smittium sparsum, and Smittium verticillatum, and the following two Stachylina spp.: Stachylina brevicellaris and Stachylina subgrandis. Four of the other 13 Harpellales, Pennella digitata, Smittium megazygosporum, Stachylina penetralis, and Zancudomyces culisetae are reported for the first time in Atlantic Canada. Also recorded is Paramoebidiumcurvum, with many more specimens of this genus from various locations and hosts included as Paramoebidium spp. only. We suggest that future collections of Diptera, to further document and discover Trichomycetes, are warranted across the varied host habitats that abound not only in eastern Canada but the rest of the country as well.
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new species of spartiella and legeriosimilis from mayflies and other arthropod associated Trichomycetes from nova scotia canada
Botany, 2012Co-Authors: Merlin M White, D B StrongmanAbstract:The digestive tracts of non-predaceous, aquatic insects and other arthropods living in moist habitats harbour a group of fungal and protistan microorganisms known as Trichomycetes or “gut fungi”. A...
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new species and first records of Trichomycetes from immature aquatic insects in idaho
Mycologia, 2012Co-Authors: Molly E Bench, Merlin M WhiteAbstract:Trichomycetes, or gut fungi, are currently recognized as an ecological group of fungi and protists that inhabit the guts of immature insects or other stages and types of arthropods. The geographic distribution of these endosymbionts is worldwide. However trichomycete data from the Pacific Northwest are limited and this is the first account of gut fungi in Idaho. We report on the Trichomycetes from a single site, Cottonwood Creek at Military Reserve Park, Boise, Idaho, where periodic surveys for more than a year resulted in the discovery of four newly named, three probably new but unnamed and 15 previously known species. Among the Harpellales three new species, Capniomyces sasquatchoides, Harpella torus and Lancisporomyces lampetriformis, are described, with two possibly new species of Smittium detailed but unnamed at this time pending further collections. A Genistelloides cf. hibernus also is included as a possible new species. One new species of Amoebidiales, Paramoebidium hamatum, is described as well. ...
Matias J Cafaro - One of the best experts on this subject based on the ideXlab platform.
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resolving relationships at the animal fungal divergence a molecular phylogenetic study of the protist Trichomycetes ichthyosporea eccrinida
Molecular Phylogenetics and Evolution, 2017Co-Authors: Nicole Reynolds, Matias J Cafaro, Matthew E Smith, Eric D Tretter, Justin Gause, Dustin Heeney, James F Smith, Stephen J Novak, William A Bourland, Merlin M WhiteAbstract:Abstract Trichomycetes is a group of microorganisms that was considered a class of fungi comprising four orders of commensal, gut-dwelling endosymbionts obligately associated with arthropods. Since molecular phylogenies revealed two of those orders (Amoebidiales and Eccrinales = “protist trichos”) to be closely related to members of the protist class Ichthyosporea (=Mesomycetozoea), Trichomycetes have been considered an ecological association of both early-diverging fungi and protists. Understanding of the taxonomy, evolution, and diversity of the protist trichos is lacking largely due to the difficulties inherent in species collection that have contributed to undersampling and understudy. The most recent classification divides the protist trichos between two families, Amoebidiidae and Eccrinidae (suborder Trichomycina, order Eccrinida). However, there is no comprehensive molecular phylogeny available for this group and major questions about the systematics of protist trichos remain unanswered. Therefore, we generated 18S and 28S rDNA sequences for 106 protist tricho samples and combined them with publicly available Eccrinida sequences for phylogenetic analyses. We also sequenced a conserved protein-coding gene (heat-shock 70 protein) to obtain a multigene data set. We conducted ancestral state reconstruction (ASR) and Bayesian tip-association significance test (BaTS) analyses by mapping six morphological and ecological characters onto the resulting phylogenetic trees. Our results demonstrate: (1) several ecological and morphological character states (habitat, host type, host stage at time of infestation, location within host, spore production, and growth form) are significantly correlated with the phylogeny, and (2) two additional protist tricho families should be incorporated into the taxonomy to reflect phylogenetic relationships. Our data suggest that an integrated strategy that combines morphological, ecological, and molecular characters is needed to further resolve and clarify the systematics of the Eccrinida.
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eccrinales Trichomycetes are not fungi but a clade of protists at the early divergence of animals and fungi
Molecular Phylogenetics and Evolution, 2005Co-Authors: Matias J CafaroAbstract:Abstract The morphologically diverse orders Eccrinales and Amoebidiales have been considered members of the fungal class Trichomycetes (Zygomycota) for the last 50 years. These organisms either inhabit the gut or are ectocommensals on the exoskeleton of a wide range of arthropods—Crustacea, Insecta, and Diplopoda—in varied habitats. The taxonomy of both orders is based on a few micromorphological characters. One species, Amoebidium parasiticum, has been axenically cultured and this has permitted several biochemical and phylogenetic analyses. As a consequence, the order Amoebidiales has been removed from the Trichomycetes and placed in the class Mesomycetozoea. An affinity between Eccrinales and Amoebidiales was first suggested when the class Trichomycetes was erected by Duboscq et al. [Arch. Zool. Exp. Gen. 86 (1948) 29]. Subsequently, molecular markers have been developed to study the relationship of these orders to other groups. Ribosomal gene (18S and 28S) sequence analyses generated by this study do not support a close association of these orders to the Trichomycetes or to other fungi. Rather, Eccrinales share a common ancestry with the Amoebidiales and belong to the protist class Mesomycetozoea, placed at the animal–fungi boundary.
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arthropod gut fungi from puerto rico and summary of tropical Trichomycetes worldwide
Caribbean Journal of Science, 2000Co-Authors: Merlin M White, Matias J Cafaro, Robert W. LichtwardtAbstract:This is the first survey of Trichomycetes in Puerto Rico. Various arthropods (including mil- lipedes, beetles, larval dipterans, isopods, and crabs) were collected from terrestrial, freshwater and marine habitats, and their gut fungi identified. The four orders of the class Trichomycetes were represented, in- cluding the following genera: Amoebidium, Asellaria, Enterobryus, Genistellospora, Harpella, Leidyomyces, Paramoebidium, Smittium and Stachylina. These correspond to 60 % (9/15) of the genera previously reported from tropical regions worldwide. Results of this survey are combined with the previous reports of tropical Trichomycetes and summarized as a species list. The Puerto Rican genera are discussed, highlighting features of particular interest to those considering future studies on Trichomycetes. We highlight the potential severe underestimation of the diversity of tropical Trichomycetes and suggest that further surveys are warranted, especially with the current focus on biodiversity and tropical microfungi.
D B Strongman - One of the best experts on this subject based on the ideXlab platform.
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new species and geographical distribution of Trichomycetes gut fungi in a novel host caddisfly trichoptera
Botany, 2019Co-Authors: D B Strongman, Merlin M WhiteAbstract:Trichomycetes are fungi and protists living in the guts of arthropods. Herbivorous insects in freshwater habitats commonly house these microbes, and there are over 300 species described. One very c...
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New trichomycete species from China and additional information on Gauthieromyces.
Mycologia, 2015Co-Authors: D B Strongman, Juan WangAbstract:We describe three new species of gut fungi (Kickxellomycotina, Harpellales) from aquatic insects, including Caudomyces typhella from a crane fly larva and Genistelloides torrentis and Orphella sinica from stonefly nymphs. Details on trichomycete species previously reported from Asia, including China, are provided. Recent descriptions of two new species of Gauthieromyces provide a more complete understanding of the genus; it is emended to reflect this new information and an epitype is designated. The description of G. indicus is emended based on an examination of the type specimen and additional collections of this species from China.
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Trichomycetes associated with insects in lotic habitats (streams) within the Halifax Regional Municipality, Nova Scotia, Canada
Botany, 2013Co-Authors: R.t. William, D B StrongmanAbstract:Reported from marine, freshwater, and terrestrial habitats, Trichomycetes are an ecological group of both fungi and protists that colonize the guts of insects, crustaceans, and millipedes. Lotic sites (streams, rivers) are a rich source of hosts and their Trichomycetes. As part of a larger study of Trichomycetes from different aquatic habitats within the Halifax Regional Municipality in Nova Scotia, Canada, one stream from each of three separate watersheds was sampled for aquatic insects, and their guts were dissected and examined for microscopic Trichomycetes. Over the course of 17 months, 24 species of Trichomycetes were collected including the following four new species: Glotzia gemina sp. nov. R.T. William and Strongman from a mayfly (Baetidae); Stachylina tanysoma sp. nov. R.T. William and Strongman; Smittium ampliboja sp. nov. R.T. William and Strongman; and Smittium insolitum sp. nov. R.T. William and Strongman, all recovered from dipteran (Chironomidae) hosts. New continental records were established for three species including Smittium pusillum Manier & Coste and Stachylina euthena Manier & Coste, both originally described from France, and Smittium nodifixum Strongman & Shengquan Xu previously known only from China. A new regional geographical record for Pennella arctica Lichtw. & M.C. Williams is reported and 17 other species previously known from eastern Canada were recorded. Pteromaktron timberleaense R.T. William & Strongman was described by us from Timberlea, Nova Scotia, but at the time no sexual spores (zygospores) were seen. In the collections reported in this paper, zygospores were found so the original description is emended to include the zygospore features for this species. This report adds to the published data on Trichomycetes from Nova Scotia that have appeared over the last 10 years and highlights the significance of long-term sampling in cataloguing the diversity of this group of insect associated microorganisms. We also present some data on seasonal variation in trichomycete occurrence and comment on site specificity in the Trichomycetes we collected.
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Trichomycetes from Governor's Lake and Lake Micmac within the Halifax Regional Municipality, Nova Scotia, Canada
Botany, 2013Co-Authors: R.t. William, D B StrongmanAbstract:Trichomycetes are endosymbiotic fungi and protists that attach to the gut lining of insects and other arthropods. The vast majority of Trichomycetes have been reported from lotic habitats (primarily flowing streams), whereas lentic habitats are relatively unexplored. Despite being largely understudied, lentic waters should be viable habitats for Trichomycetes because insect types commonly colonized by Trichomycetes in lotic systems are also found in lentic habitats. As part of a larger study, two lakes in separate watersheds within the Halifax Regional Municipality, Nova Scotia, Canada, were sampled. Six species of Trichomycetes were found, including three new species, Smittium guttisporum sp. nov. R.T. William and Strongman, Stachylina serpula sp. nov. R.T. William and Strongman, and Stachylina zeppelin sp. nov. R.T. William and Strongman, all isolated from dipteran (Chironomidae) hosts. A new continental record for Smittium bulbosporophorus L.G. Valle & Santam., previously only reported from Spain, is documented from Governor's Lake, Timberlea, Nova Scotia. Smittium dipterorum Lichtw., known from Costa Rica, Dominican Republic, and Mexico was also collected from the Governor's Lake site, establishing a new Canadian record.
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Trichomycetes occurring in both lentic lake and lotic stream habitats within the halifax regional municipality nova scotia canada1
Botany, 2013Co-Authors: R.t. William, D B StrongmanAbstract:Trichomycetes are an ecological group of fungi and protists found inside the gut lumen of aquatic insect larvae and have been reported virtually everywhere trichomycetologists have looked for them. Trichomycetes are common in freshwater aquatic habitats, both lentic and lotic, though lentic habitats have been explored less frequently. An inventory of Trichomycetes inhabiting both stream (lotic) and lake (lentic) habitats was conducted within the Halifax Regional Municipality, Nova Scotia, Canada, including one site at the interface where a stream emptied into a lake. Twenty-six species of Trichomycetes were collected from both lotic and lentic sites, nine of which are new species, all isolated from midge (Chironomidae) larvae. These are as follows: Smittium adaiosporum sp. nov. R.T. William and Strongman, Smittium cryptancora sp. nov. R.T. William and Strongman , Smittium petilum sp. nov. R.T. William and Strongman, Smittium peculiare sp. nov. R.T. William and Strongman, Stachylina abundans sp. nov. R.T. William and Strongman, Stachylina extensiva sp. nov. R.T. William and Strongman, Stachylina infrequens sp. nov. R.T. William and Strongman, Stachylina somnisimilis sp. nov. R.T. William and Strongman, and Stachylina uranus sp. nov. R.T. William and Strongman. In addition, a new continental record for Smittium hecatei L.G. Valle & Santam. previously reported from Spain and new regional geographic records for Smittium minutisporum Lichtw., Siri & M.M. White and Smittium mucronatum Manier & Mathiez ex Manier are documented. In addition, 14 other Trichomycetes previously reported from Atlantic Canada were recorded and these included both harpellid fungi and protists in the genus Paramoebidium. Insects from the orders Diptera, Ephemeroptera, and Plecoptera were all found to contain Trichomycetes. Data on the frequency of occurrence and the habitat preference for some gut fungi are discussed.
