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Kyung-soo Hahm - One of the best experts on this subject based on the ideXlab platform.
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antifungal mechanism of smap 29 1 18 isolated from sheep myeloid mrna against Trichosporon Beigelii
Biochemical and Biophysical Research Communications, 2002Co-Authors: Dong Gun Lee, Pyoung Il Kim, Yoonkyung Park, Seongcheol Park, Eunrhan Woo, Kyung-soo HahmAbstract:The antifungal activity and mechanism of SMAP-29 (1-18) (SMAP-29), a cathelicidin-derived antimicrobial peptide deduced from N-terminal sequence of sheep myeloid mRNA, were investigated. SMAP-29 displayed a strong antifungal activity against various fungi. To understand the antifungal mechanism(s) of SMAP-29, we examined the interaction of SMAP-29 with the pathogenic fungus Trichosporon Beigelii. Confocal microscopy showed that SMAP-29 was localized in the plasma membrane. The antifungal effects of SMAP-29 were further confirmed by using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a plasma membrane probe. Flow cytometric analysis revealed that SMAP-29 acted in an energy-dependent manner. This interaction is also dependent on the ionic environment. Furthermore, SMAP-29 caused significant morphological changes when testing the membrane disrupting activity using liposomes (phosphatidylcholine/cholesterol; 10:1, w/w), as shown by scanning electron microscopy. The results suggest that SMAP-29 may exert its antifungal activity by disrupting the structure of cell membranes, via direct interaction with the lipid bilayers and irregularly disrupted fungal membranes in an energy- and salt-dependent manner.
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Inflence on the cell membrane of Trichosporon Beigelii by fungicidal peptide derived from cecropin A(1-8)-magainin 2(1-12)
1999Co-Authors: Dong Gun Lee, Song Yub Shin, Cheol Young Maeng, Kyung-soo HahmAbstract:The fungicidal activities of the cecropin A(1-8)-magainin 2(1-12) (CA- MA) and its analogues with amino acid substitution were measured by a growth inhibition assay using Trichosporon Beigelii. CA-MA displayed potent fungicidal activity (minimal inhibitory concentration: 5 μg/ml) against Trichosporon Beigelii with no hemolytic activity. Substitution (A12-CA-MA: analogue 2) of Ala to Lys at position 12 of CA-MA caused an increase in fungicidal activity without any increase in hemolytic activity. In order to investigate the fungicidal mechanism of analogue 2, it was reacted with Trichosporon Beigelii protoplasts. The morphological changes and the failure of the cell wall regeneration of the protoplast by analogue 2 were observed. Moreover, the amounts of released potassium ion by the peptide were increased. These results suggested that the fungicidal mechanism of analogue 2 may be due to the pore formation or the detergent-like disruption of cell membranes.ope
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Cecropin A-melittin hybrid peptide exerts its antifungal effects by damaging on the plasma membranes of Trichosporon Beigelii
Biotechnology Letters, 1998Co-Authors: Dong Gun Lee, Song Yub Shin, Cheol Young Maeng, Kyung-soo HahmAbstract:To elucidate the effect of the peptide derived from cecropin A(1-8)-melittin(1-12) having potent antifungal activity without cytotoxicity against eukaryotic cell on the fungal cell membranes, Trichosporon Beigelii protoplasts were prepared. The protoplasts treated with the peptide not only failed to regenerate the fungal cell walls but also disrupted the membrane, indicating that the peptide exerts its antifungal activity by acting on the plasma membranes. © Rapid Science Ltd. 1998
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Cecropin A-melittin hybrid peptide exerts its antifungal effects by damaging on the plasma membranes of Trichosporon Beigelii
Springer Verlag (Germany), 1998Co-Authors: Dong Gun Lee, Song Yub Shin, Cheol Young Maeng, Kyung-soo HahmAbstract:To elucidate the effect of the peptide derived from cecropin A(1-8)-melittin(1-12) having potent antifungal activity without cytotoxicity against eukaryotic cell on the fungal cell membranes, Trichosporon Beigelii protoplasts were prepared. The protoplasts treated with the peptide not only failed to regenerate the fungal cell walls but also disrupted the membrane, indicating that the peptide exerts its antifungal activity by acting on the plasma membranes.ope
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Antifungal Activities of Magainin-2 Hybrid Peptides against Trichosporon Beigelii
Journal of Microbiology and Biotechnology, 1997Co-Authors: Dong Gun Lee, Song Yub Shin, Sung Gu Lee, Kil Lyong Kim, Myung Kyu Lee, Kyung-soo HahmAbstract:In order to obtain a hybrid synthetic peptide with a more potent antifungal activity than magainin-2 but without hemolytic activity, four hybrid peptides were designed from the sequences of magainin 2 and cecropin A and their antifungal activities against Trichosporon Beigelii were investigated. The result showed that analogue 2 and 4 exhibited better antifungal activity against T. Beigelii than magainin-2 but no hemolytic activities. The peptides, therefore, could be used as models for the development of potent antifungal peptides.
Dong Gun Lee - One of the best experts on this subject based on the ideXlab platform.
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antifungal mechanism of smap 29 1 18 isolated from sheep myeloid mrna against Trichosporon Beigelii
Biochemical and Biophysical Research Communications, 2002Co-Authors: Dong Gun Lee, Pyoung Il Kim, Yoonkyung Park, Seongcheol Park, Eunrhan Woo, Kyung-soo HahmAbstract:The antifungal activity and mechanism of SMAP-29 (1-18) (SMAP-29), a cathelicidin-derived antimicrobial peptide deduced from N-terminal sequence of sheep myeloid mRNA, were investigated. SMAP-29 displayed a strong antifungal activity against various fungi. To understand the antifungal mechanism(s) of SMAP-29, we examined the interaction of SMAP-29 with the pathogenic fungus Trichosporon Beigelii. Confocal microscopy showed that SMAP-29 was localized in the plasma membrane. The antifungal effects of SMAP-29 were further confirmed by using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a plasma membrane probe. Flow cytometric analysis revealed that SMAP-29 acted in an energy-dependent manner. This interaction is also dependent on the ionic environment. Furthermore, SMAP-29 caused significant morphological changes when testing the membrane disrupting activity using liposomes (phosphatidylcholine/cholesterol; 10:1, w/w), as shown by scanning electron microscopy. The results suggest that SMAP-29 may exert its antifungal activity by disrupting the structure of cell membranes, via direct interaction with the lipid bilayers and irregularly disrupted fungal membranes in an energy- and salt-dependent manner.
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Inflence on the cell membrane of Trichosporon Beigelii by fungicidal peptide derived from cecropin A(1-8)-magainin 2(1-12)
1999Co-Authors: Dong Gun Lee, Song Yub Shin, Cheol Young Maeng, Kyung-soo HahmAbstract:The fungicidal activities of the cecropin A(1-8)-magainin 2(1-12) (CA- MA) and its analogues with amino acid substitution were measured by a growth inhibition assay using Trichosporon Beigelii. CA-MA displayed potent fungicidal activity (minimal inhibitory concentration: 5 μg/ml) against Trichosporon Beigelii with no hemolytic activity. Substitution (A12-CA-MA: analogue 2) of Ala to Lys at position 12 of CA-MA caused an increase in fungicidal activity without any increase in hemolytic activity. In order to investigate the fungicidal mechanism of analogue 2, it was reacted with Trichosporon Beigelii protoplasts. The morphological changes and the failure of the cell wall regeneration of the protoplast by analogue 2 were observed. Moreover, the amounts of released potassium ion by the peptide were increased. These results suggested that the fungicidal mechanism of analogue 2 may be due to the pore formation or the detergent-like disruption of cell membranes.ope
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Cecropin A-melittin hybrid peptide exerts its antifungal effects by damaging on the plasma membranes of Trichosporon Beigelii
Biotechnology Letters, 1998Co-Authors: Dong Gun Lee, Song Yub Shin, Cheol Young Maeng, Kyung-soo HahmAbstract:To elucidate the effect of the peptide derived from cecropin A(1-8)-melittin(1-12) having potent antifungal activity without cytotoxicity against eukaryotic cell on the fungal cell membranes, Trichosporon Beigelii protoplasts were prepared. The protoplasts treated with the peptide not only failed to regenerate the fungal cell walls but also disrupted the membrane, indicating that the peptide exerts its antifungal activity by acting on the plasma membranes. © Rapid Science Ltd. 1998
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Cecropin A-melittin hybrid peptide exerts its antifungal effects by damaging on the plasma membranes of Trichosporon Beigelii
Springer Verlag (Germany), 1998Co-Authors: Dong Gun Lee, Song Yub Shin, Cheol Young Maeng, Kyung-soo HahmAbstract:To elucidate the effect of the peptide derived from cecropin A(1-8)-melittin(1-12) having potent antifungal activity without cytotoxicity against eukaryotic cell on the fungal cell membranes, Trichosporon Beigelii protoplasts were prepared. The protoplasts treated with the peptide not only failed to regenerate the fungal cell walls but also disrupted the membrane, indicating that the peptide exerts its antifungal activity by acting on the plasma membranes.ope
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Antifungal Activities of Magainin-2 Hybrid Peptides against Trichosporon Beigelii
Journal of Microbiology and Biotechnology, 1997Co-Authors: Dong Gun Lee, Song Yub Shin, Sung Gu Lee, Kil Lyong Kim, Myung Kyu Lee, Kyung-soo HahmAbstract:In order to obtain a hybrid synthetic peptide with a more potent antifungal activity than magainin-2 but without hemolytic activity, four hybrid peptides were designed from the sequences of magainin 2 and cecropin A and their antifungal activities against Trichosporon Beigelii were investigated. The result showed that analogue 2 and 4 exhibited better antifungal activity against T. Beigelii than magainin-2 but no hemolytic activities. The peptides, therefore, could be used as models for the development of potent antifungal peptides.
D V Gokhale - One of the best experts on this subject based on the ideXlab platform.
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a practical and scalable process for 4 r hydroxycyclopent 2 en 1 s acetate by desymmetrization of meso cyclopent 2 en 1 4 diacetate catalyzed by Trichosporon Beigelii ncim 3326 a cheap biocatalyst
Tetrahedron-asymmetry, 2000Co-Authors: Uttam R Kalkote, Sandeep R Ghorpade, Rohini R Joshi, T Ravindranathan, Kulbhushan B Bastawade, D V GokhaleAbstract:Abstract Various yeast and fungal cultures from NCIM, NCL, Pune, India were screened for the hydrolysis of meso -cyclopent-2-en-1,4-diacetate 2 to 4-( R )-hydroxycyclopen-2-en-1-( S )-acetate 1 to provide a cheaper and more effective alternative to PLE which is currently being used for the conversion. Yeast cultures of Trichosporon species were identified as having a pro- R preference in the hydrolysis of 2 ; but the enantioselectivity was poor. Hence detailed medium-engineering investigations were made for the hydrolysis of 2 to 1 using a culture of Trichosporon Beigelii (NCIM 3326) as catalyst. Addition of 10% v/v ethanol was found to enhance the enantioselectivity of the enzyme, affording 1 of 85% optical purity (op) in 83% yield. Further exploration of inherent consecutive kinetic resolutions to the desymmetrization afforded 1 of >98% op in 74% chemical yield.
Yoichiro Goto - One of the best experts on this subject based on the ideXlab platform.
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in vitro susceptibility of Trichosporon Beigelii to antifungal agents
Journal of Chemotherapy, 1996Co-Authors: K Perparim, Yoichiro Goto, T Tashiro, H Nagai, Atsuro Hashimoto, M NasuAbstract:The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of amphotericin B, flucytosine, miconazole, fluconazole and itraconazole against 21 isolates of Trichosporon Beigelii in RPMI-1640 medium were determined using National Committee for Clinical Laboratory Standards (NCCLS) methodology in microdilution method. Most isolates were sensitive to miconazole (MIC90 0.78 microgram/ml), fluconazole (MIC90 6.25 micrograms/ml), and itraconazole (MIC90 0.19 microgram/ml), with the former being the most active agent tested (MFC90 3.12 mu/ml). Although amphotericin B inhibited most strains (MIC range, 0.78-3.12 micrograms/ml), poor fungicidal activity was observed (MFC range, 1.56-12.5 micrograms/ml) showing a pattern of relative resistance in vitro. Flucytosine showed generally poor activity against most isolates tested. These in vitro findings confirm the resistance of T.Beigelii to amphotericin B and suggest that azoles may be an alternative to the former for the treatment of disseminated Trichosporonosis. However, in vivo studies would better validate these in vitro findings.
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Trichosporon Beigelii pneumonia in patients with hematologic malignancies
Chest, 1995Co-Authors: Takayoshi Tashiro, Hiroyuki Nagai, Yoichiro Goto, Perparim Kamberi, Hiroshi Nagaoka, Masaru NasuAbstract:Trichosporon Beigelii is a causative agent of hypersensitivity pneumonia in immunocompetent individuals and of invasive pneumonia in immunocompromised patients. The actual incidence and clinical manifestations of T Beigelii pneumonia are obscure because the diagnosis is sometimes difficult. We studied eight patients with T Beigelii pneumonia diagnosed by immunohistochemical investigation of lung tissue sections and/or isolation of the organism from the lung, sputum, or blood. All patients had underlying hematologic malignancies for which they had received cytotoxic chemotherapy, resulting in profound neutropenia. The clinical manifestations were persistent fever unresponsive to broad-spectrum antibiotic therapy, cough, bloody sputum, and rapidly progressive dyspnea. The chest radiographs showed diffuse alveolar infiltrates in four patients, diffuse interstitial infiltrates in one, patchy reticulonodular infiltrates in one, and focal alveolar infiltrates in two. Histopathologic examination demonstrated numerous centrally necrotic foci with minimal cellular inflammatory reaction, intra-alveolar hemorrhage, and edema. Trichosporon Beigelii consisting of both yeast and hyphal forms was located predominantly in the alveolar vessels. In neutropenic patients with hematologic malignancies, this fungus appears to enter the lung not only through the airways but also via the hematogenous route. In vitro susceptibility testing indicated borderline susceptibility to amphotericin B and showed that some azoles were active against T Beigelii at safely achievable serum concentrations.
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disseminated Trichosporon Beigelii infection in patients with malignant diseases immunohistochemical study and review
European Journal of Clinical Microbiology & Infectious Diseases, 1994Co-Authors: T Tashiro, Yoichiro Goto, H Nagai, P Kamberi, H Kikuchi, M Nasu, Shin-ichro AkizukiAbstract:Trichosporon Beigelii is a causative agent of opportunistic infection and summer-type hypersensitivity pneumonitis in Japan. However, as the diagnosis of Trichosporon Beigelii infection is sometimes difficult, the actual incidence of this disease may be underestimated. Of 203 autopsy patients with malignant disease, seven (7.7%) were diagnosed with disseminated Trichosporon Beigelii infection by immunohistochemical investigation of formalin-fixed, paraffin-embedded tissue sections. Including these seven, a total of 43 patients with Trichosporon Beigelii infection have been reported in Japan. The majority of them had underlying hematologic malignancies, for which they received cytotoxic chemotherapy resulting in neutropenia. This study indicates that the immunohistochemical method, which can be applied to biopsy specimens, is an excellent tool for specific diagnosis of Trichosporon Beigelii infection, which is an emerging fatal mycosis in immunocompromised patients with profound neutropenia.
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Disseminated Trichosporon Beigelii infection: report of nine cases and review
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, 1993Co-Authors: Takayoshi Tashiro, Hiroyuki Nagai, Tohru Yamasaki, Yoichiro Goto, Shin-ichro Akizuki, Masaru NasuAbstract:Trichosporon Beigelii (formerly called Trichosporon cutaneum) is an emerging pathogen of disseminated Trichosporonosis in immunocompromised patients. We conducted postmortem microbiological examinations of lung aspirates and blood in the heart. T. Beigelii was isolated from the samples of 7 (2.24%) of 313 patients. Five of 7 patients isolated were diagnosed as having Trichosporonosis based on histochemical and clinical findings. The other two were considered as colonization. Immunohistochemical study using Cryptococcus neoformans-absorbed antiserum to T. Beigelii was carried out on the sections of autopsied tissues with deep mycoses. The sections from seven patients were positively stained, which were formerly diagnosed as candidiasis and/or aspergillosis. Consequently, 9 patients had disseminated Trichosporonosis caused by T. Beigelii. In Japan, 43 patients including the present 9, 31 males and 12 females, aged 51 (range, 2-84), were reported in the literature. Thirty-seven (86%) patients had hematologic malignancy, and the majority of them revealed profound neutropenia due to cytotoxic chemotherapy. Thirty-eight (88%) patients died despite the anti-fungal chemotherapy including amphotericin B. New strategies for refractory disseminated Trichosporonosis in immunocompromised patients is needed.
Shin-ichro Akizuki - One of the best experts on this subject based on the ideXlab platform.
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disseminated Trichosporon Beigelii infection in patients with malignant diseases immunohistochemical study and review
European Journal of Clinical Microbiology & Infectious Diseases, 1994Co-Authors: T Tashiro, Yoichiro Goto, H Nagai, P Kamberi, H Kikuchi, M Nasu, Shin-ichro AkizukiAbstract:Trichosporon Beigelii is a causative agent of opportunistic infection and summer-type hypersensitivity pneumonitis in Japan. However, as the diagnosis of Trichosporon Beigelii infection is sometimes difficult, the actual incidence of this disease may be underestimated. Of 203 autopsy patients with malignant disease, seven (7.7%) were diagnosed with disseminated Trichosporon Beigelii infection by immunohistochemical investigation of formalin-fixed, paraffin-embedded tissue sections. Including these seven, a total of 43 patients with Trichosporon Beigelii infection have been reported in Japan. The majority of them had underlying hematologic malignancies, for which they received cytotoxic chemotherapy resulting in neutropenia. This study indicates that the immunohistochemical method, which can be applied to biopsy specimens, is an excellent tool for specific diagnosis of Trichosporon Beigelii infection, which is an emerging fatal mycosis in immunocompromised patients with profound neutropenia.
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Disseminated Trichosporon Beigelii infection: report of nine cases and review
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, 1993Co-Authors: Takayoshi Tashiro, Hiroyuki Nagai, Tohru Yamasaki, Yoichiro Goto, Shin-ichro Akizuki, Masaru NasuAbstract:Trichosporon Beigelii (formerly called Trichosporon cutaneum) is an emerging pathogen of disseminated Trichosporonosis in immunocompromised patients. We conducted postmortem microbiological examinations of lung aspirates and blood in the heart. T. Beigelii was isolated from the samples of 7 (2.24%) of 313 patients. Five of 7 patients isolated were diagnosed as having Trichosporonosis based on histochemical and clinical findings. The other two were considered as colonization. Immunohistochemical study using Cryptococcus neoformans-absorbed antiserum to T. Beigelii was carried out on the sections of autopsied tissues with deep mycoses. The sections from seven patients were positively stained, which were formerly diagnosed as candidiasis and/or aspergillosis. Consequently, 9 patients had disseminated Trichosporonosis caused by T. Beigelii. In Japan, 43 patients including the present 9, 31 males and 12 females, aged 51 (range, 2-84), were reported in the literature. Thirty-seven (86%) patients had hematologic malignancy, and the majority of them revealed profound neutropenia due to cytotoxic chemotherapy. Thirty-eight (88%) patients died despite the anti-fungal chemotherapy including amphotericin B. New strategies for refractory disseminated Trichosporonosis in immunocompromised patients is needed.
