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H Hoste - One of the best experts on this subject based on the ideXlab platform.
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anthelmintic activities against haemonchus contortus or Trichostrongylus colubriformis from small ruminants are influenced by structural features of condensed tannins
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Jessica Quijada, Christos Fryganas, Honorata M Ropiak, Aina Ramsay, Irene Muellerharvey, H HosteAbstract:Plants containing condensed tannins (CTs) may hold promise as alternatives to synthetic anthelmintic (AH) drugs for controlling gastrointestinal nematodes (GINs). However, the structural features that contribute to the AH activities of CTs remain elusive. This study probed the relationships between CT structures and their AH activities. Eighteen plant resources were selected on the basis of their diverse CT structures. From each plant resource, two CT fractions were isolated and their in vitro AH activities were measured with the larval exsheathment inhibition assay, which was applied to Haemonchus contortus and Trichostrongylus colubriformis. Calculation of mean EC50 values indicated that H. contortus was more susceptible than T. colubriformis to the different fractions and that the F1 fractions were less efficient than the F2 ones, as indicated by the respective mean values for H. contortus, F1 = 136.9 ± 74.1 μg/mL and F2 = 108.1 ± 53.2 μg/mL, and for T. colubriformis, F1 = 233 ± 54.3 μg/mL and F2 = 166...
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in vitro effect of heather extracts on Trichostrongylus colubriformis eggs larvae and adults
Veterinary Parasitology, 2013Co-Authors: Javier Morenogonzalo, H Hoste, F Manolaraki, Pilar Frutos, Gonzalo Hervas, R Celaya, K Osoro, L M OrtegamoraAbstract:Abstract This study was carried out to evaluate the in vitro effects of different heather species on Trichostrongylus colubriformis eggs, larvae and adult worms, and obtain scientific evidence to attribute these effects to the action of their phenolic compounds and/or tannins. Total phenolic extracts of three heather species (Calluna vulgaris, Erica cinerea, and Erica umbellata) and an equal mixture of these three extracts were tested in vitro in the three development stages of T. colubriformis using an egg hatching assay (EHA), larval exsheathment inhibition assay (LEIA), and adult motility inhibition assay (AMIA). The egg hatching rate was measured after incubation with heather extracts for 48 h at 25 °C. Infective third-stage larvae (L3) were incubated for 3 h at 25 °C with heather extracts. The evolution of artificial exsheathment over time was measured with repeated observations at 20-min intervals for 60 min. Adult worms were obtained from one donor goat and incubated with the extracts at 37 °C for 5 days in 48-multiwell plates. Worm motility was measured at 0, 19, 24, 43, 48, 67, 72, 96 and 115 h after the beginning of the experiment. The extracts were tested at concentrations of 75, 150, 300, 600 and 1200 μg/ml. All extracts significantly (P
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effects of four tropical tanniniferous plant extracts on the inhibition of larval migration and the exsheathment process of Trichostrongylus colubriformis infective stage
Veterinary Parasitology, 2008Co-Authors: M A Alonsodiaz, J F J Torresacosta, C A Sandovalcastro, C M Capetilloleal, S Brunet, H HosteAbstract:Abstract The anthelmintic (AH) effect of Acacia pennatula, Leucaena leucocephala, Lisyloma latisiliquum and Piscidia piscipula was evaluated in the infective larvae (L3) of Trichostrongylus colubriformis. Different concentrations of lyophilized extracts were tested using the larval migration inhibition (LMI) test. An inhibitor of tannins (the polyvinyl polypyrrolidone [PVPP]) was used to verify whether these compounds were responsible for the AH effects. Then, the effect of extracts on larval exsheathment was examined by observing the exsheathment process at 10-min intervals for 70 min. The LMI test showed a dose-dependant AH effect for A. pennatula, L. leucocephala and L. latisiliquum (P
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effects of four tropical tanniniferous plant extracts on the inhibition of larval migration and the exsheathment process of Trichostrongylus colubriformis infective stage
Veterinary Parasitology, 2008Co-Authors: M A Alonsodiaz, J F J Torresacosta, C A Sandovalcastro, C M Capetilloleal, S Brunet, H HosteAbstract:The anthelmintic (AH) effect of Acacia pennatula, Leucaena leucocephala, Lisyloma latisiliquum and Piscidia piscipula was evaluated in the infective larvae (L(3)) of Trichostrongylus colubriformis. Different concentrations of lyophilized extracts were tested using the larval migration inhibition (LMI) test. An inhibitor of tannins (the polyvinyl polypyrrolidone [PVPP]) was used to verify whether these compounds were responsible for the AH effects. Then, the effect of extracts on larval exsheathment was examined by observing the exsheathment process at 10-min intervals for 70 min. The LMI test showed a dose-dependant AH effect for A. pennatula, L. leucocephala and L. latisiliquum (P<0.01), but not for P. piscipula. The restoration of L(3) migration to values similar to those of controls after the addition of PVPP, indicates that tannins are involved in AH effects. Trichostrongylus colubriformis exsheathment was partially or totally blocked by the four plants extracts. Tropical tanniniferous plants evaluated in the current study may have potential as AH for the control of T. colubriformis if in vivo investigations indicate useful effects.
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experimental concurrent infection of sheep with oestrus ovis and Trichostrongylus colubriformis effects of antiparasitic treatments on interactions between parasite populations and blood eosinophilic responses
Veterinary Parasitology, 2006Co-Authors: H.t. Yacob, Jean Paul Bergeaud, Christelle Grisez, Ph Jacquiet, H Hoste, G Terefe, Francoise Prevot, Ph DorchiesAbstract:Abstract The purpose of this experiment was to determine if an earlier infection with Oestrus ovis would down regulate an infection with Trichostrongylus colubriformis when the larvae of O. ovis were expelled from the nasal cavities of sheep by a specific treatment. Three groups of five lambs were used: group 1 was artificially infected with O. ovis larvae and later with T. colubriformis , group 2 received O. ovis larvae and later was treated with ivermectin 14 days before being infected with T. colubriformis . Group 3 was infected with T. colubriformis only. The criteria examined were: the effects on nematode egg excretion, worm fecundity, nematode burdens and the kinetics of blood eosinophils. Significant decreases of nematode egg excretion, worm fecundity, nematode burdens were observed in group 1 compared to group 3. However, no changes were observed in either group 2 or 3. In group 2 it was noted that antiparasitic treatment induced a rapid decrease in blood eosinophils to a range close to the non-infected control group and this was associated with the removal of the down regulation effects of nematode burdens. This experiment showed that there is no cross immunity between O. ovis and T. colubriformis and that eosinophils may act against any parasite without specific priming.
M Y Hasegawa - One of the best experts on this subject based on the ideXlab platform.
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dried ground banana plant leaves musa spp for the control of haemonchus contortus and Trichostrongylus colubriformis infections in sheep
Parasitology Research, 2015Co-Authors: Lilian Gregory, Eidi Yoshihara, Bruno Leonardo Mendonca Ribeiro, L K F Silva, Eduardo Marques, E B S Meira, R S Rossi, P H Sampaio, H Louvandini, M Y HasegawaAbstract:To evaluate the anthelmintic effect of Musa spp. leaves, 12 animals were artificially infected with Haemonchus contortus, and another 12 animals were infected with Trichostrongylus colubriformis. Then, both treatment groups were offered 400 g of dried ground banana plant leaves, and the control animals were offered only 1000 g of coast cross hay. During the trials, the animals received weekly physical examinations. The methods used to evaluate the efficiency of this treatment were packed cell volume, total plasma protein and faecal egg counts, and egg hatchability tests were performed on days −2, +3, +6, +9, +13 and +15. Coproculture tests were performed on day −2 to confirm monospecific infections. In the FEC and EHT, a statistically significant difference (0.04, 0.005; p 0.05) for Haemochus contortus group in all tests. Our results confirmed previous findings suggesting that dried ground banana plant leaves possess anthelmintic activity.
Lilian Gregory - One of the best experts on this subject based on the ideXlab platform.
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dried ground banana plant leaves musa spp for the control of haemonchus contortus and Trichostrongylus colubriformis infections in sheep
Parasitology Research, 2015Co-Authors: Lilian Gregory, Eidi Yoshihara, Bruno Leonardo Mendonca Ribeiro, L K F Silva, Eduardo Marques, E B S Meira, R S Rossi, P H Sampaio, H Louvandini, M Y HasegawaAbstract:To evaluate the anthelmintic effect of Musa spp. leaves, 12 animals were artificially infected with Haemonchus contortus, and another 12 animals were infected with Trichostrongylus colubriformis. Then, both treatment groups were offered 400 g of dried ground banana plant leaves, and the control animals were offered only 1000 g of coast cross hay. During the trials, the animals received weekly physical examinations. The methods used to evaluate the efficiency of this treatment were packed cell volume, total plasma protein and faecal egg counts, and egg hatchability tests were performed on days −2, +3, +6, +9, +13 and +15. Coproculture tests were performed on day −2 to confirm monospecific infections. In the FEC and EHT, a statistically significant difference (0.04, 0.005; p 0.05) for Haemochus contortus group in all tests. Our results confirmed previous findings suggesting that dried ground banana plant leaves possess anthelmintic activity.
Herve Hoste - One of the best experts on this subject based on the ideXlab platform.
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ellagitannins inhibit the exsheathment of haemonchus contortus and Trichostrongylus colubriformis larvae the efficiency increases together with the molecular size
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Maari Karone, Jussi Suvanto, Marica T Engstrom, Jeffrey R Ahe, Lucie Legrou, Jari Sinkkone, Juhapekka Salmine, Herve HosteAbstract:Worldwide, parasitic gastrointestinal nematodes continue to threaten animal health, welfare, and production in outdoor breeding systems of small ruminants. For more than 50 years, the control of these parasitic worms has relied on the use of commercial synthetic anthelmintics. However, anthelmintic resistance in worm populations is nowadays widespread and requires novel solutions. The use of tannin-rich plants has been suggested as an alternative to synthetic anthelmintics to control gastrointestinal nematodes. The majority of previous studies have focused on the activity of proanthocyanidins (syn condensed tannins), and less is known about ellagitannins. In this study, the effects of 30 structurally unique ellagitannins on the exsheathment of third-stage infective larvae were examined on Haemonchus contortus and Trichostrongylus colubriformis by the in vitro larval exsheathment inhibition assay. Ellagitannins were found to be promising natural anthelmintics as they showed direct inhibition on larval exsheathment for both nematode species. In general, ellagitannins were more efficient at inhibiting the exsheathment of H. contortus larvae than those of T. colubriformis. The efficiency of inhibition increased as the degree of oligomerization or the molecular weight of the ellagitannin increased. Otherwise, we found no other structural features of ellagitannins that significantly affected the anthelmintic activity on the third-stage infective larvae. The effective concentrations were physiologically relevant and should be achievable in the gastrointestinal tract also in in vivo conditions.
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Anthelmintic Activities against Haemonchus contortus or Trichostrongylus colubriformis from small ruminants are influenced by structural features of condensed tannins
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Jessica Quijada, Christos Fryganas, Honorata M Ropiak, Aina Ramsay, Irene Mueller Harvey, Herve HosteAbstract:Plants containing condensed tannins (CTs) may hold promise as alternatives to synthetic anthelmintic (AH) drugs for controlling gastrointestinal nematodes (GINs). However, the structural features that contribute to the AH activities of CTs remain elusive. This study probed the relationships between CT structures and their AH activities. Eighteen plant resources were selected on the basis of their diverse CT structures. From each plant resource, two CT fractions were isolated and their in vitro AH activities were measured with the larval exsheathment inhibition assay, which was applied to Haemonchus contortus and Trichostrongylus colubriformis. Calculation of mean EC50 values indicated that H. contortus was more susceptible than T. colubriformis to the different fractions and that the F1 fractions were less efficient than the F2 ones, as indicated by the respective mean values for H. contortus, F1 = 136.9 +/- 74.1 mu g/mL and F2 = 108.1 +/- 53.2 mu g/mL, and for T. colubriformis, F1 = 233 +/- 54.3 mu g/mL and F2 = 166 +/- 39.9 mu g/mL. The results Showed that the AH activity against H. contortus was associated with the monomeric Si units that give rise to prodelphinidins (P < 0.05) and with CT polymer size (P < 0.10). However, for T. colubriformis AH activity Was correlated only with prodelphinidins (P < 0.05). These results suggest that CTs have different modes of action against different parasite species.
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concurrent parasitic infections of sheep depression of Trichostrongylus colubriformis populations by a subsequent infection with oestrus ovis
Veterinary Parasitology, 2004Co-Authors: H.t. Yacob, Céline Bleuart, Jean Paul Bergeaud, Christelle Grisez, Ph Jacquiet, Frédéric Prévot, Ph Dorchies, Herve HosteAbstract:Abstract Concurrent infections of sheep with Oestrus ovis and trichostrongyles of the digestive tract are common in the field. Previous results have shown that a previous infection with O. ovis adversely affects worm populations of either Trichostrongylus colubriformis or Haemonchus contortus. However, no information was available to determine the influence of the succession of infections on the expression of interactions between these parasites located in remote anatomical sites. In order to investigate the role of these modulating factors, an experimental study was conducted on four groups of naive sheep, examining the consequences of a delayed infection with O. ovis on a pre-existing population of T. colubriformis. group T was infected four times with 4000 T. colubriformis larvae on days 0, 14, 28 and 42 of experiment; group O received multiple infections with O. ovis first instar larvae on days 42, 49, 56, 70 and 77; sheep from group TO received both infections and animals from group C remained as uninfected controls. Faecal egg counts and eosinophilia were measured weekly throughout the study. At necropsy (day 91), the mucosal cellular responses in the nasal cavities (septum, turbinates, ethmoid and sinus) and in the digestive tract (stomach and small intestine) from all animals were analysed from histological sections. Infection of the digestive tract with nematodes did not modify the biology of Oestrus populations, as measured by the number and weight of larvae. In contrast, infections with O. ovis after T. colubriformis infection was related to significant reductions (P
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effects of condensed tannins on established populations and on incoming larvae of Trichostrongylus colubriformis and teladorsagia circumcincta in goats
Veterinary Research, 2003Co-Authors: Virginie Paolini, Audrey Frayssines, Philippe Dorchies, Herve HosteAbstract:The use of tanniferous plants or tannins represents one alternative approach to the control of gastrointestinal parasites in ruminants but most data have been obtained in sheep. The current study was therefore performed in goats with two objectives: firstly, to investigate the effects of condensed tannins (CT) on adult populations of Trichostrongylus colubriformis and Teladorsa- gia circumcincta; secondly, to examine their effects on the establishment of infective larvae of these two species. In experiment 1, two groups of kids were infected with 6 000 L3 of T. colubriformis and 6 000 L3 of T. circumcincta. After 7 weeks, quebracho extracts were administered per os for 8 days to one group. A comparable group which did not receive tannins was included as the control. The kids were slaughtered on week 11. Parasitological and pathophysiological parameters were measured weekly. Worm counts were assessed and mast cells, globule leukocytes and eosinophils were counted in the abomasal and intestinal mucosae. Tannin administration was associated with a decrease in egg excretion, and a decrease in female fecundity, but with no changes in worm num- bers. These changes were associated with an increased number of intestinal mast cells. In experi- ment 2, 24 goats were used according to a 2 2 factorial design, depending on infection and tannin administration. Two groups were either infected with 6 000 L3 of T. colubriformis or T. circum- cincta. Within each group, the goats were either drenched or undrenched with tannin extracts. Pathophysiological parameters were measured weekly. Twelve days after the cessation of tannin administration, the goats were slaughtered. Worm counts and female worm fecundity were deter- mined. Tannin consumption was associated with a significant reduction (P < 0.001) of Trichos- trongylus populations and a close to significant reduction for Teladorsagia. No effect on fecundity was observed. Our results (1) confirm the consequences of condensed tannins on nematodes in goats as in sheep and (2) indicate divergent effects depending on the parasitic stage exposed to the con- densed tannins. Trichostrongylus colubriformis / Teladorsagia circumcincta / goats / tannin / alternative methods of control
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comparison of the effects on milk production of concurrent infection with haemonchus contortus and Trichostrongylus colubriformis in high and low producing dairy goats
American Journal of Veterinary Research, 1993Co-Authors: Herve Hoste, C ChartierAbstract:The impact of nematode parasitism of the digestive tract on milk output and milk quality was examined in dairy goats. In addition, the consequences of worm infection were compared in goats with different lactation performance (ie, with initial high or low milk production). Forty-eight goats in the second month of lactation were allotted equally to 2 groups. The first group was given 5,000 Haemonchus contortus and 20,000 Trichostrongylus colubriformis larvae. The 24 additional goats remained free of parasites. Parasitologic, serologic, and milk data were collected every 2 weeks for 5 months, and body condition of the goats was scored throughout the study. Results of strongyle egg count in feces, increase in pepsinogen values, and reduction in RBC count, PCV, and serum inorganic phosphate concentration indicated subclinical infection. This subclinical parasitism induced a decrease in body condition scoring and led to persistent decrease in milk yield, ranging from 2.5 to 10% reduction from control values. Changes in fat and protein contents were not detected. In contrast, the consequences of infection were more severe in the 6 goats with the highest milk production at the start of the study. Decrease in milk output ranged between 13.0 to 25.1%, and was associated with decrease in fat content. Comparison of the response to parasitism in the 6 goats with the highest lactation performance and the 6 goats with the lowest performance indicated differences between both subgroups. According to parasitologic and pathologic data, high-producer goats had less resistance and/or resilience to infection associated with more severe consequences on milk production.
M A Alonsodiaz - One of the best experts on this subject based on the ideXlab platform.
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effects of four tropical tanniniferous plant extracts on the inhibition of larval migration and the exsheathment process of Trichostrongylus colubriformis infective stage
Veterinary Parasitology, 2008Co-Authors: M A Alonsodiaz, J F J Torresacosta, C A Sandovalcastro, C M Capetilloleal, S Brunet, H HosteAbstract:Abstract The anthelmintic (AH) effect of Acacia pennatula, Leucaena leucocephala, Lisyloma latisiliquum and Piscidia piscipula was evaluated in the infective larvae (L3) of Trichostrongylus colubriformis. Different concentrations of lyophilized extracts were tested using the larval migration inhibition (LMI) test. An inhibitor of tannins (the polyvinyl polypyrrolidone [PVPP]) was used to verify whether these compounds were responsible for the AH effects. Then, the effect of extracts on larval exsheathment was examined by observing the exsheathment process at 10-min intervals for 70 min. The LMI test showed a dose-dependant AH effect for A. pennatula, L. leucocephala and L. latisiliquum (P
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effects of four tropical tanniniferous plant extracts on the inhibition of larval migration and the exsheathment process of Trichostrongylus colubriformis infective stage
Veterinary Parasitology, 2008Co-Authors: M A Alonsodiaz, J F J Torresacosta, C A Sandovalcastro, C M Capetilloleal, S Brunet, H HosteAbstract:The anthelmintic (AH) effect of Acacia pennatula, Leucaena leucocephala, Lisyloma latisiliquum and Piscidia piscipula was evaluated in the infective larvae (L(3)) of Trichostrongylus colubriformis. Different concentrations of lyophilized extracts were tested using the larval migration inhibition (LMI) test. An inhibitor of tannins (the polyvinyl polypyrrolidone [PVPP]) was used to verify whether these compounds were responsible for the AH effects. Then, the effect of extracts on larval exsheathment was examined by observing the exsheathment process at 10-min intervals for 70 min. The LMI test showed a dose-dependant AH effect for A. pennatula, L. leucocephala and L. latisiliquum (P<0.01), but not for P. piscipula. The restoration of L(3) migration to values similar to those of controls after the addition of PVPP, indicates that tannins are involved in AH effects. Trichostrongylus colubriformis exsheathment was partially or totally blocked by the four plants extracts. Tropical tanniniferous plants evaluated in the current study may have potential as AH for the control of T. colubriformis if in vivo investigations indicate useful effects.
