The Experts below are selected from a list of 6159 Experts worldwide ranked by ideXlab platform
Geoffrey A Cordell - One of the best experts on this subject based on the ideXlab platform.
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lc esi ms study of the flavonoid glycoside malonates of red clover Trifolium pratense
Journal of Agricultural and Food Chemistry, 2000Co-Authors: Longze Lin, Michael Lindenmaier, Jie Yang, Michael Cleary, Shengxiang Qiu, Geoffrey A CordellAbstract:High-performance liquid chromatography−electrospray ionization−mass spectrometry (LC-ESI-MS) was applied to the analysis of the flavonoids and their glycoside malonates of the flowers and leaves of red clover (Trifolium pratense). Through LC-MS comparative studies on the plant extracts and their malonate-free extracts, ∼20 flavonoid glycoside malonates were detected in the flower extract. Eight were identified as genistin 6‘ ‘-O-malonate (39), formononetin 7-O-β-d-glucoside 6‘ ‘-O-malonate (40), biochanin A 7-O-β-d-glucoside 6‘ ‘-O-malonate (41), trifoside 6‘ ‘-O-malonate (42), irilone 4‘-O-β-d-glucoside 6‘ ‘-O-malonate (43), pratensein 7-O-β-d-glucoside 6‘ ‘-O-malonate (44), isoquercitrin 6‘ ‘-O-malonate (45), and 3-methylquercetin 7-O-β-d-glucoside 6‘ ‘-O-malonate (46). About 15 other flavonoids and clovamides were proved to be present in this extract. The study also found that the flowers contained flavones as the major flavonoids, whereas the leaves had isoflavones as the major flavonoids. This is the...
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lc esi ms study of the flavonoid glycoside malonates of red clover Trifolium pratense
Journal of Agricultural and Food Chemistry, 2000Co-Authors: Longze Lin, Michael Lindenmaier, Jie Yang, Michael Cleary, Shengxiang Qiu, Geoffrey A CordellAbstract:High-performance liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was applied to the analysis of the flavonoids and their glycoside malonates of the flowers and leaves of red clover (Trifolium pratense). Through LC-MS comparative studies on the plant extracts and their malonate-free extracts, approximately 20 flavonoid glycoside malonates were detected in the flower extract. Eight were identified as genistin 6' '-O-malonate (39), formononetin 7-O-beta-D-glucoside 6' '-O-malonate (40), biochanin A 7-O-beta-D-glucoside 6' '-O-malonate (41), trifoside 6' '-O-malonate (42), irilone 4'-O-beta-D-glucoside 6' '-O-malonate (43), pratensein 7-O-beta-D-glucoside 6' '-O-malonate (44), isoquercitrin 6' '-O-malonate (45), and 3-methylquercetin 7-O-beta-D-glucoside 6' '-O-malonate (46). About 15 other flavonoids and clovamides were proved to be present in this extract. The study also found that the flowers contained flavones as the major flavonoids, whereas the leaves had isoflavones as the major flavonoids. This is the first detection of the six malonates (39 and 42-46) in the extracts of red clover, and among them, 42, 43, and 46 are new compounds.
Liebao Han - One of the best experts on this subject based on the ideXlab platform.
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analysis of transcripts and splice isoforms in red clover Trifolium pratense l by single molecule long read sequencing
BMC Plant Biology, 2018Co-Authors: Yuehui Chao, Jianbo Yuan, Siqiao Jia, Liebao HanAbstract:Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is the most widely planted forage legume after alfalfa. Although a draft genome sequence was published already, the sequences and completed structure of mRNA transcripts remain unclear, which limit further explore on red clover. In this study, the red clover transcriptome was sequenced using single-molecule long-read sequencing to identify full-length splice isoforms, and 29,730 novel isoforms from known genes and 2194 novel isoforms from novel genes were identified. A total of 5492 alternative splicing events was identified and the majority of alter spliced events in red clover was corrected as intron retention. In addition, of the 15,229 genes detected by SMRT, 8719 including 186,517 transcripts have at least one poly(A) site. Furthermore, we identified 4333 long non-coding RNAs and 3762 fusion transcripts. We analyzed full-length transcriptome of red clover with PacBio SMRT. Those new findings provided important information for improving red clover draft genome annotation and fully characterization of red clover transcriptome.
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analysis of transcripts and splice isoforms in red clover Trifolium pratense l by single molecule long read sequencing
bioRxiv, 2018Co-Authors: Yuehui Chao, Jianbo Yuan, Siqiao Jia, Liebao HanAbstract:Abstract Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is the most widely planted forage legume after alfalfa. Although a draft genome sequence was published already, the sequences and completed structure of mRNA transcripts remain unclear, which limit further explore on red clover. In this study, the red clover transcriptome was sequenced using single-molecule long-read sequencing to identify full-length splice isoforms, and 29,730 novel isoforms from known genes and 2,194 novel isoforms from novel genes were identified. A total of 5,492 alternative splicing events was identified and the majority of alter spliced events in red clover was corrected as intron retention. In addition, of the 15,229 genes detected by SMRT, 8,719 including 1,86,517 transcripts have at least one poly(A) site. Furthermore, we identified 4,333 long non-coding RNAs and 3,762 fusion transcripts. Our results show the feasibility of deep sequencing full-length RNA from red clover transcriptome on a single-molecule level.
Longze Lin - One of the best experts on this subject based on the ideXlab platform.
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lc esi ms study of the flavonoid glycoside malonates of red clover Trifolium pratense
Journal of Agricultural and Food Chemistry, 2000Co-Authors: Longze Lin, Michael Lindenmaier, Jie Yang, Michael Cleary, Shengxiang Qiu, Geoffrey A CordellAbstract:High-performance liquid chromatography−electrospray ionization−mass spectrometry (LC-ESI-MS) was applied to the analysis of the flavonoids and their glycoside malonates of the flowers and leaves of red clover (Trifolium pratense). Through LC-MS comparative studies on the plant extracts and their malonate-free extracts, ∼20 flavonoid glycoside malonates were detected in the flower extract. Eight were identified as genistin 6‘ ‘-O-malonate (39), formononetin 7-O-β-d-glucoside 6‘ ‘-O-malonate (40), biochanin A 7-O-β-d-glucoside 6‘ ‘-O-malonate (41), trifoside 6‘ ‘-O-malonate (42), irilone 4‘-O-β-d-glucoside 6‘ ‘-O-malonate (43), pratensein 7-O-β-d-glucoside 6‘ ‘-O-malonate (44), isoquercitrin 6‘ ‘-O-malonate (45), and 3-methylquercetin 7-O-β-d-glucoside 6‘ ‘-O-malonate (46). About 15 other flavonoids and clovamides were proved to be present in this extract. The study also found that the flowers contained flavones as the major flavonoids, whereas the leaves had isoflavones as the major flavonoids. This is the...
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lc esi ms study of the flavonoid glycoside malonates of red clover Trifolium pratense
Journal of Agricultural and Food Chemistry, 2000Co-Authors: Longze Lin, Michael Lindenmaier, Jie Yang, Michael Cleary, Shengxiang Qiu, Geoffrey A CordellAbstract:High-performance liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was applied to the analysis of the flavonoids and their glycoside malonates of the flowers and leaves of red clover (Trifolium pratense). Through LC-MS comparative studies on the plant extracts and their malonate-free extracts, approximately 20 flavonoid glycoside malonates were detected in the flower extract. Eight were identified as genistin 6' '-O-malonate (39), formononetin 7-O-beta-D-glucoside 6' '-O-malonate (40), biochanin A 7-O-beta-D-glucoside 6' '-O-malonate (41), trifoside 6' '-O-malonate (42), irilone 4'-O-beta-D-glucoside 6' '-O-malonate (43), pratensein 7-O-beta-D-glucoside 6' '-O-malonate (44), isoquercitrin 6' '-O-malonate (45), and 3-methylquercetin 7-O-beta-D-glucoside 6' '-O-malonate (46). About 15 other flavonoids and clovamides were proved to be present in this extract. The study also found that the flowers contained flavones as the major flavonoids, whereas the leaves had isoflavones as the major flavonoids. This is the first detection of the six malonates (39 and 42-46) in the extracts of red clover, and among them, 42, 43, and 46 are new compounds.
Yuehui Chao - One of the best experts on this subject based on the ideXlab platform.
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analysis of transcripts and splice isoforms in red clover Trifolium pratense l by single molecule long read sequencing
BMC Plant Biology, 2018Co-Authors: Yuehui Chao, Jianbo Yuan, Siqiao Jia, Liebao HanAbstract:Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is the most widely planted forage legume after alfalfa. Although a draft genome sequence was published already, the sequences and completed structure of mRNA transcripts remain unclear, which limit further explore on red clover. In this study, the red clover transcriptome was sequenced using single-molecule long-read sequencing to identify full-length splice isoforms, and 29,730 novel isoforms from known genes and 2194 novel isoforms from novel genes were identified. A total of 5492 alternative splicing events was identified and the majority of alter spliced events in red clover was corrected as intron retention. In addition, of the 15,229 genes detected by SMRT, 8719 including 186,517 transcripts have at least one poly(A) site. Furthermore, we identified 4333 long non-coding RNAs and 3762 fusion transcripts. We analyzed full-length transcriptome of red clover with PacBio SMRT. Those new findings provided important information for improving red clover draft genome annotation and fully characterization of red clover transcriptome.
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analysis of transcripts and splice isoforms in red clover Trifolium pratense l by single molecule long read sequencing
bioRxiv, 2018Co-Authors: Yuehui Chao, Jianbo Yuan, Siqiao Jia, Liebao HanAbstract:Abstract Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is the most widely planted forage legume after alfalfa. Although a draft genome sequence was published already, the sequences and completed structure of mRNA transcripts remain unclear, which limit further explore on red clover. In this study, the red clover transcriptome was sequenced using single-molecule long-read sequencing to identify full-length splice isoforms, and 29,730 novel isoforms from known genes and 2,194 novel isoforms from novel genes were identified. A total of 5,492 alternative splicing events was identified and the majority of alter spliced events in red clover was corrected as intron retention. In addition, of the 15,229 genes detected by SMRT, 8,719 including 1,86,517 transcripts have at least one poly(A) site. Furthermore, we identified 4,333 long non-coding RNAs and 3,762 fusion transcripts. Our results show the feasibility of deep sequencing full-length RNA from red clover transcriptome on a single-molecule level.
Michael Lindenmaier - One of the best experts on this subject based on the ideXlab platform.
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lc esi ms study of the flavonoid glycoside malonates of red clover Trifolium pratense
Journal of Agricultural and Food Chemistry, 2000Co-Authors: Longze Lin, Michael Lindenmaier, Jie Yang, Michael Cleary, Shengxiang Qiu, Geoffrey A CordellAbstract:High-performance liquid chromatography−electrospray ionization−mass spectrometry (LC-ESI-MS) was applied to the analysis of the flavonoids and their glycoside malonates of the flowers and leaves of red clover (Trifolium pratense). Through LC-MS comparative studies on the plant extracts and their malonate-free extracts, ∼20 flavonoid glycoside malonates were detected in the flower extract. Eight were identified as genistin 6‘ ‘-O-malonate (39), formononetin 7-O-β-d-glucoside 6‘ ‘-O-malonate (40), biochanin A 7-O-β-d-glucoside 6‘ ‘-O-malonate (41), trifoside 6‘ ‘-O-malonate (42), irilone 4‘-O-β-d-glucoside 6‘ ‘-O-malonate (43), pratensein 7-O-β-d-glucoside 6‘ ‘-O-malonate (44), isoquercitrin 6‘ ‘-O-malonate (45), and 3-methylquercetin 7-O-β-d-glucoside 6‘ ‘-O-malonate (46). About 15 other flavonoids and clovamides were proved to be present in this extract. The study also found that the flowers contained flavones as the major flavonoids, whereas the leaves had isoflavones as the major flavonoids. This is the...
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lc esi ms study of the flavonoid glycoside malonates of red clover Trifolium pratense
Journal of Agricultural and Food Chemistry, 2000Co-Authors: Longze Lin, Michael Lindenmaier, Jie Yang, Michael Cleary, Shengxiang Qiu, Geoffrey A CordellAbstract:High-performance liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was applied to the analysis of the flavonoids and their glycoside malonates of the flowers and leaves of red clover (Trifolium pratense). Through LC-MS comparative studies on the plant extracts and their malonate-free extracts, approximately 20 flavonoid glycoside malonates were detected in the flower extract. Eight were identified as genistin 6' '-O-malonate (39), formononetin 7-O-beta-D-glucoside 6' '-O-malonate (40), biochanin A 7-O-beta-D-glucoside 6' '-O-malonate (41), trifoside 6' '-O-malonate (42), irilone 4'-O-beta-D-glucoside 6' '-O-malonate (43), pratensein 7-O-beta-D-glucoside 6' '-O-malonate (44), isoquercitrin 6' '-O-malonate (45), and 3-methylquercetin 7-O-beta-D-glucoside 6' '-O-malonate (46). About 15 other flavonoids and clovamides were proved to be present in this extract. The study also found that the flowers contained flavones as the major flavonoids, whereas the leaves had isoflavones as the major flavonoids. This is the first detection of the six malonates (39 and 42-46) in the extracts of red clover, and among them, 42, 43, and 46 are new compounds.
