The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Harald G Wiker - One of the best experts on this subject based on the ideXlab platform.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry. In total, 1417 different proteins were identified. In silico analysis of the identified proteins revealed that 248 proteins had at least one predicted trans-membrane region. Also, 64 of the identified proteins were predicted lipoproteins, and 54 proteins were predicted as outer membrane proteins. Three-hundred-and-ninety-five of the observed proteins, including 91 integral membrane proteins were described for the first time. Comparison of abundance levels of the identified proteins was performed using the eXponentially modified protein abundance indeX (emPAI) which takes into account the number of the observable peptides to the number of eXperimentally observed peptide ions for a given protein. The outcome showed that among the membrane-and membrane-associated proteins several proteins are present with high relative abundance. Further, a close eXamination of the lipoprotein LpqG (Rv3623) which is only detected in the membrane fractions of M. tuberculosis but not in M. bovis, revealed that the homologous gene in M. bovis lack the signal peptide and lipoboX motif, suggesting impaired eXport to the membrane. Altogether, we have identified a substantial proportion of membrane- and membrane-associated proteins of M. tuberculosis H37Rv, compared the relative abundance of the identified proteins and also revealed subtle differences between the different members of the M. tuberculosis compleX.
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membrane and membrane associated proteins in Triton X 114 eXtracts of mycobacterium bovis bcg identified using a combination of gel based and gel free fractionation strategies
Proteomics, 2008Co-Authors: Hiwa Malen, Tina Softeland, Gustavo A De Souza, Harald G Wiker, Frode S Berven, Magnus O Arntzen, Clive DsantosAbstract:Tuberculosis is an ancient disease that remains a significant global health problem. Because many membrane and membrane-associated proteins of this pathogen represent potential targets for drugs, diagnostic probes or vaccine components, we have analysed Mycobacterium bovis, bacillus Calmette-Guerin (BCG) substrain Moreau, using Triton X-114 for eXtraction of lipophilic proteins, followed by identification with LC coupled MS/MS. We identified 351 different proteins in total, and 103 (29%) were predicted as integral membrane proteins with at least one predicted transmembrane region and another 84 (23.9%) proteins had a positive grand average of hydropathicity (GRAVY) value, indicating increased probability for membrane association. Altogether 43 predicted lipoproteins (Lpps) were identified which is close to 50% of the total number of Lpps in the genome. Fifty-four proteins, including twenty-four predicted integral membrane proteins and seven predicted Lpps are described for the first time. The proportion of hydrophobic membrane and membrane-associated proteins shows that Triton X-114 is a highly efficient method for eXtraction of membrane proteins from bacteria, without the need for preisolation of membranes. ATP synthase, NAD(P) transhydrogenase, ubiquinone oXidoreductase and ubiquinol-cytochrome C reductase appear to represent major enzyme compleXes in the membrane of Mycobacterium tuberculosis compleX organisms.
Hiwa Malen - One of the best experts on this subject based on the ideXlab platform.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry. In total, 1417 different proteins were identified. In silico analysis of the identified proteins revealed that 248 proteins had at least one predicted trans-membrane region. Also, 64 of the identified proteins were predicted lipoproteins, and 54 proteins were predicted as outer membrane proteins. Three-hundred-and-ninety-five of the observed proteins, including 91 integral membrane proteins were described for the first time. Comparison of abundance levels of the identified proteins was performed using the eXponentially modified protein abundance indeX (emPAI) which takes into account the number of the observable peptides to the number of eXperimentally observed peptide ions for a given protein. The outcome showed that among the membrane-and membrane-associated proteins several proteins are present with high relative abundance. Further, a close eXamination of the lipoprotein LpqG (Rv3623) which is only detected in the membrane fractions of M. tuberculosis but not in M. bovis, revealed that the homologous gene in M. bovis lack the signal peptide and lipoboX motif, suggesting impaired eXport to the membrane. Altogether, we have identified a substantial proportion of membrane- and membrane-associated proteins of M. tuberculosis H37Rv, compared the relative abundance of the identified proteins and also revealed subtle differences between the different members of the M. tuberculosis compleX.
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membrane and membrane associated proteins in Triton X 114 eXtracts of mycobacterium bovis bcg identified using a combination of gel based and gel free fractionation strategies
Proteomics, 2008Co-Authors: Hiwa Malen, Tina Softeland, Gustavo A De Souza, Harald G Wiker, Frode S Berven, Magnus O Arntzen, Clive DsantosAbstract:Tuberculosis is an ancient disease that remains a significant global health problem. Because many membrane and membrane-associated proteins of this pathogen represent potential targets for drugs, diagnostic probes or vaccine components, we have analysed Mycobacterium bovis, bacillus Calmette-Guerin (BCG) substrain Moreau, using Triton X-114 for eXtraction of lipophilic proteins, followed by identification with LC coupled MS/MS. We identified 351 different proteins in total, and 103 (29%) were predicted as integral membrane proteins with at least one predicted transmembrane region and another 84 (23.9%) proteins had a positive grand average of hydropathicity (GRAVY) value, indicating increased probability for membrane association. Altogether 43 predicted lipoproteins (Lpps) were identified which is close to 50% of the total number of Lpps in the genome. Fifty-four proteins, including twenty-four predicted integral membrane proteins and seven predicted Lpps are described for the first time. The proportion of hydrophobic membrane and membrane-associated proteins shows that Triton X-114 is a highly efficient method for eXtraction of membrane proteins from bacteria, without the need for preisolation of membranes. ATP synthase, NAD(P) transhydrogenase, ubiquinone oXidoreductase and ubiquinol-cytochrome C reductase appear to represent major enzyme compleXes in the membrane of Mycobacterium tuberculosis compleX organisms.
Sharad Pathak - One of the best experts on this subject based on the ideXlab platform.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry. In total, 1417 different proteins were identified. In silico analysis of the identified proteins revealed that 248 proteins had at least one predicted trans-membrane region. Also, 64 of the identified proteins were predicted lipoproteins, and 54 proteins were predicted as outer membrane proteins. Three-hundred-and-ninety-five of the observed proteins, including 91 integral membrane proteins were described for the first time. Comparison of abundance levels of the identified proteins was performed using the eXponentially modified protein abundance indeX (emPAI) which takes into account the number of the observable peptides to the number of eXperimentally observed peptide ions for a given protein. The outcome showed that among the membrane-and membrane-associated proteins several proteins are present with high relative abundance. Further, a close eXamination of the lipoprotein LpqG (Rv3623) which is only detected in the membrane fractions of M. tuberculosis but not in M. bovis, revealed that the homologous gene in M. bovis lack the signal peptide and lipoboX motif, suggesting impaired eXport to the membrane. Altogether, we have identified a substantial proportion of membrane- and membrane-associated proteins of M. tuberculosis H37Rv, compared the relative abundance of the identified proteins and also revealed subtle differences between the different members of the M. tuberculosis compleX.
Anna Taraba - One of the best experts on this subject based on the ideXlab platform.
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properties of aqueous solutions of nonionic surfactants Triton X 114 and tween 80 at temperatures from 293 to 318 k spectroscopic and ultrasonic studies
Chemical Physics, 2017Co-Authors: Katarzyna Szymczyk, Anna TarabaAbstract:Abstract The speed of sound, density and refractive indeX of aqueous solutions of the nonionic surfactants, p-(1,1,3,3-tetramethylbutyl) phenoXypoly(ethyleneglycol) Triton X-114 (TX114) and polysorbate 80, Tween 80, have been measured over the entire range of concentration at 293, 298, 303, 308, 313 and 318 K under atmospheric pressure. Steady state fluorescence measurements have been also made using pyrene as a probe. From the eXperimental data the quantities such as critical micelle concentration (CMC), apparent dielectric constant, hydration number, isentropic compressibility, apparent specific adiabatic compressibility of a solute, intermolecular free length, acoustic impedance and molar sound number were determined. The variation of these parameters with concentration and temperature was discussed in terms of intermolecular interactions in the solution of a given surfactant.
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aggregation behavior of Triton X 114 and tween 80 at various temperatures and concentrations studied by density and viscosity measurements
Journal of Thermal Analysis and Calorimetry, 2016Co-Authors: Katarzyna Szymczyk, Anna TarabaAbstract:Measurements of density and viscosity for the aqueous solutions of the nonionic surfactants, Triton X-114 and Tween 80, have been taken in the temperature range T = 293–318 K at an interval of 5 K. On the basis of the obtained results, various parameters have been calculated and analyzed including concentration of surfactants in the bulk phase and temperature, for eXample volume eXpansivity and fractional free volume, which indicates that water around the Tween 80 molecules is more loosely bound than the Triton X-114. Moreover, it was proved that depending on the temperature and concentration of studied surfactants they can behave as a water structure breaker or maker.
Gustavo A De Souza - One of the best experts on this subject based on the ideXlab platform.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry.
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definition of novel cell envelope associated proteins in Triton X 114 eXtracts of mycobacterium tuberculosis h37rv
BMC Microbiology, 2010Co-Authors: Hiwa Malen, Sharad Pathak, Tina Softeland, Gustavo A De Souza, Harald G WikerAbstract:Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 detergent-phase separation for eXtraction of lipophilic proteins, followed by their identification with high resolution mass spectrometry. In total, 1417 different proteins were identified. In silico analysis of the identified proteins revealed that 248 proteins had at least one predicted trans-membrane region. Also, 64 of the identified proteins were predicted lipoproteins, and 54 proteins were predicted as outer membrane proteins. Three-hundred-and-ninety-five of the observed proteins, including 91 integral membrane proteins were described for the first time. Comparison of abundance levels of the identified proteins was performed using the eXponentially modified protein abundance indeX (emPAI) which takes into account the number of the observable peptides to the number of eXperimentally observed peptide ions for a given protein. The outcome showed that among the membrane-and membrane-associated proteins several proteins are present with high relative abundance. Further, a close eXamination of the lipoprotein LpqG (Rv3623) which is only detected in the membrane fractions of M. tuberculosis but not in M. bovis, revealed that the homologous gene in M. bovis lack the signal peptide and lipoboX motif, suggesting impaired eXport to the membrane. Altogether, we have identified a substantial proportion of membrane- and membrane-associated proteins of M. tuberculosis H37Rv, compared the relative abundance of the identified proteins and also revealed subtle differences between the different members of the M. tuberculosis compleX.
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membrane and membrane associated proteins in Triton X 114 eXtracts of mycobacterium bovis bcg identified using a combination of gel based and gel free fractionation strategies
Proteomics, 2008Co-Authors: Hiwa Malen, Tina Softeland, Gustavo A De Souza, Harald G Wiker, Frode S Berven, Magnus O Arntzen, Clive DsantosAbstract:Tuberculosis is an ancient disease that remains a significant global health problem. Because many membrane and membrane-associated proteins of this pathogen represent potential targets for drugs, diagnostic probes or vaccine components, we have analysed Mycobacterium bovis, bacillus Calmette-Guerin (BCG) substrain Moreau, using Triton X-114 for eXtraction of lipophilic proteins, followed by identification with LC coupled MS/MS. We identified 351 different proteins in total, and 103 (29%) were predicted as integral membrane proteins with at least one predicted transmembrane region and another 84 (23.9%) proteins had a positive grand average of hydropathicity (GRAVY) value, indicating increased probability for membrane association. Altogether 43 predicted lipoproteins (Lpps) were identified which is close to 50% of the total number of Lpps in the genome. Fifty-four proteins, including twenty-four predicted integral membrane proteins and seven predicted Lpps are described for the first time. The proportion of hydrophobic membrane and membrane-associated proteins shows that Triton X-114 is a highly efficient method for eXtraction of membrane proteins from bacteria, without the need for preisolation of membranes. ATP synthase, NAD(P) transhydrogenase, ubiquinone oXidoreductase and ubiquinol-cytochrome C reductase appear to represent major enzyme compleXes in the membrane of Mycobacterium tuberculosis compleX organisms.
