The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform
De-hua Lai - One of the best experts on this subject based on the ideXlab platform.
-
Infection with Trypanosoma Lewisi or Trypanosoma musculi may promote the spread of Toxoplasma gondii.
Parasitology, 2021Co-Authors: Jiang-mei Gao, Zhao-rong Lun, Guo-qing Geng, Geoff Hide, De-hua LaiAbstract:Toxoplasma gondii can infect almost all warm-blooded vertebrates with pathogensis being largely influenced by the host immune status. As important epidemiological hosts, rodents are globally distributed and are also commonly found infected with haemoflagellates, such as those in the genus Trypanosoma. We here address whether and how co-infection with trypanosomes can influence T. gondii infection in laboratory models. Rats of five strains, co-infected with T. Lewisi and mice of four strains, co-infected with T. musculi, were found to be more or less susceptible to T. gondii infection, respectively, with corresponding increased or decreased brain cyst burdens. Downregulation of iNOS expression and decreased NO production or reverse were observed in the peritoneal macrophages of rats or mice, infected with trypanosomes, respectively. Trypanosoma Lewisi and T. musculi can modulate host immune responses, either by enhancement or suppression and influence the outcome of Toxoplasma infection.
-
Novel organization of mitochondrial minicircles and guide RNAs in the zoonotic pathogen Trypanosoma Lewisi.
Nucleic acids research, 2020Co-Authors: Xuan Zhang, Geoff Hide, De-hua Lai, Julius Lukeš, Ju-feng Wang, Zhao-rong LunAbstract:Abstract Kinetoplastid flagellates are known for several unusual features, one of which is their complex mitochondrial genome, known as kinetoplast (k) DNA, composed of mutually catenated maxi- and minicircles. Trypanosoma Lewisi is a member of the Stercorarian group of trypanosomes which is, based on human infections and experimental data, now considered a zoonotic pathogen. By assembling a total of 58 minicircle classes, which fall into two distinct categories, we describe a novel type of kDNA organization in T. Lewisi. RNA-seq approaches allowed us to map the details of uridine insertion and deletion editing events upon the kDNA transcriptome. Moreover, sequencing of small RNA molecules enabled the identification of 169 unique guide (g) RNA genes, with two differently organized minicircle categories both encoding essential gRNAs. The unprecedented organization of minicircles and gRNAs in T. Lewisi broadens our knowledge of the structure and expression of the mitochondrial genomes of these human and animal pathogens. Finally, a scenario describing the evolution of minicircles is presented.
-
Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma Lewisi, a zoonotic pathogen.
Cell cycle (Georgetown Tex.), 2019Co-Authors: Xuan Zhang, Geoff Hide, De-hua Lai, Zhao-rong LunAbstract:Trypanosoma (Herpetosoma) Lewisi is a globally distributed rat trypanosome, currently considered as a zoonotic pathogen; however, a detailed understanding of the morphological events occurring during the cell cycle is lacking. This study aimed to investigate the cell cycle morphology and cleavage events of Trypanosoma Lewisi (T. Lewisi) during in vitro cultivation. By establishing in vitro cultivation of T. Lewisi at 37°C, various cell morphologies and stages could be observed. We have provided a quantitative analysis of the morphological events during T. Lewisi proliferation. We confirmed a generation time of 12.14 ± 0.79 hours, which is similar to that in vivo (12.21 ± 0.14 hours). We also found that there are two distinct cell cycles, with a two-way transformation connection in the developmental status of this parasite, which was contrasted with the previous model of multiple division patterns seen in T. Lewisi. We quantified the timing of cell cycle phases (G1n, 0.56 U; Sn, 0.14 U; G2n, 0.16 U; M, 0.06 U; C, 0.08 U; G1k, 0.65 U; Sk, 0.10 U; G2k, 0.17 U; D, 0.03 U; A, 0.05 U) and their morphological characteristics, particularly with respect to the position of kinetoplast(s) and nucleus/nuclei. Interestingly, we found that nuclear replication in T. Lewisi occurred prior to kinetoplast replication, different to the order of replication found in Trypanosoma brucei and Trypanosoma cruzi, implicating a distinct cell cycle control mechanism in T. Lewisi. We characterized the morphological events during the T. Lewisi cell cycle and presented evidence to support the existence of two distinct cell cycles with two-way transformation between them. These results provide insights into the differentiation and evolution of this parasite and its related species. Keywords: Trypanosoma Lewisi, Cell cycle, In vitro, Multiplication, Division, Zoonotic pathogen
Zhao-rong Lun - One of the best experts on this subject based on the ideXlab platform.
-
Infection with Trypanosoma Lewisi or Trypanosoma musculi may promote the spread of Toxoplasma gondii.
Parasitology, 2021Co-Authors: Jiang-mei Gao, Zhao-rong Lun, Guo-qing Geng, Geoff Hide, De-hua LaiAbstract:Toxoplasma gondii can infect almost all warm-blooded vertebrates with pathogensis being largely influenced by the host immune status. As important epidemiological hosts, rodents are globally distributed and are also commonly found infected with haemoflagellates, such as those in the genus Trypanosoma. We here address whether and how co-infection with trypanosomes can influence T. gondii infection in laboratory models. Rats of five strains, co-infected with T. Lewisi and mice of four strains, co-infected with T. musculi, were found to be more or less susceptible to T. gondii infection, respectively, with corresponding increased or decreased brain cyst burdens. Downregulation of iNOS expression and decreased NO production or reverse were observed in the peritoneal macrophages of rats or mice, infected with trypanosomes, respectively. Trypanosoma Lewisi and T. musculi can modulate host immune responses, either by enhancement or suppression and influence the outcome of Toxoplasma infection.
-
Novel organization of mitochondrial minicircles and guide RNAs in the zoonotic pathogen Trypanosoma Lewisi.
Nucleic acids research, 2020Co-Authors: Xuan Zhang, Geoff Hide, De-hua Lai, Julius Lukeš, Ju-feng Wang, Zhao-rong LunAbstract:Abstract Kinetoplastid flagellates are known for several unusual features, one of which is their complex mitochondrial genome, known as kinetoplast (k) DNA, composed of mutually catenated maxi- and minicircles. Trypanosoma Lewisi is a member of the Stercorarian group of trypanosomes which is, based on human infections and experimental data, now considered a zoonotic pathogen. By assembling a total of 58 minicircle classes, which fall into two distinct categories, we describe a novel type of kDNA organization in T. Lewisi. RNA-seq approaches allowed us to map the details of uridine insertion and deletion editing events upon the kDNA transcriptome. Moreover, sequencing of small RNA molecules enabled the identification of 169 unique guide (g) RNA genes, with two differently organized minicircle categories both encoding essential gRNAs. The unprecedented organization of minicircles and gRNAs in T. Lewisi broadens our knowledge of the structure and expression of the mitochondrial genomes of these human and animal pathogens. Finally, a scenario describing the evolution of minicircles is presented.
-
Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma Lewisi, a zoonotic pathogen.
Cell cycle (Georgetown Tex.), 2019Co-Authors: Xuan Zhang, Geoff Hide, De-hua Lai, Zhao-rong LunAbstract:Trypanosoma (Herpetosoma) Lewisi is a globally distributed rat trypanosome, currently considered as a zoonotic pathogen; however, a detailed understanding of the morphological events occurring during the cell cycle is lacking. This study aimed to investigate the cell cycle morphology and cleavage events of Trypanosoma Lewisi (T. Lewisi) during in vitro cultivation. By establishing in vitro cultivation of T. Lewisi at 37°C, various cell morphologies and stages could be observed. We have provided a quantitative analysis of the morphological events during T. Lewisi proliferation. We confirmed a generation time of 12.14 ± 0.79 hours, which is similar to that in vivo (12.21 ± 0.14 hours). We also found that there are two distinct cell cycles, with a two-way transformation connection in the developmental status of this parasite, which was contrasted with the previous model of multiple division patterns seen in T. Lewisi. We quantified the timing of cell cycle phases (G1n, 0.56 U; Sn, 0.14 U; G2n, 0.16 U; M, 0.06 U; C, 0.08 U; G1k, 0.65 U; Sk, 0.10 U; G2k, 0.17 U; D, 0.03 U; A, 0.05 U) and their morphological characteristics, particularly with respect to the position of kinetoplast(s) and nucleus/nuclei. Interestingly, we found that nuclear replication in T. Lewisi occurred prior to kinetoplast replication, different to the order of replication found in Trypanosoma brucei and Trypanosoma cruzi, implicating a distinct cell cycle control mechanism in T. Lewisi. We characterized the morphological events during the T. Lewisi cell cycle and presented evidence to support the existence of two distinct cell cycles with two-way transformation between them. These results provide insights into the differentiation and evolution of this parasite and its related species. Keywords: Trypanosoma Lewisi, Cell cycle, In vitro, Multiplication, Division, Zoonotic pathogen
-
A preliminary serogical study of Trypanosoma evansi and Trypanosoma Lewisi
2018Co-Authors: Jiang-mei Gao, Truc Philippe, Desquenes M., Vincendeau P., Courtois P., Xuan Zhang, Jittapalapong S., Zhao-rong LunAbstract:Trypanosoma evansi, known as an animal trypanosome, is widely distributed in many countries of Africa, Asia and South America; it causes significant economic loss in these countries. A few cases have also occurred in some countries of Europe due to the importation of infected animals from endemic regions. Rare human T. evansi infections were attended by the health departments and international health organizations in these endemic countries. Trypanosoma Lewisi, a cosmopolitan parasite of rats, sometimes found in humans, is currently considered as a zoonotic pathogen and has gained special attention from scientists and international health organizations such as the World Health Organization. The current study considered the serological screening of human infection by T. evansi and T. Lewisi in a Chinese human population. None of the 622 samples was found positive for T. evansi infection using the card agglutination test for the trypanosome antigen Rotat 1.2, while, 2.41% of the examined serum samples exhibited some seropositivity to T. Lewisi using enzyme-linked immunosorbent assay. No significant difference was found between the samples from areas in the South (Zhaoqing, Guangdong) and Central (Zhengzhou, Henan) China
Geoff Hide - One of the best experts on this subject based on the ideXlab platform.
-
Infection with Trypanosoma Lewisi or Trypanosoma musculi may promote the spread of Toxoplasma gondii.
Parasitology, 2021Co-Authors: Jiang-mei Gao, Zhao-rong Lun, Guo-qing Geng, Geoff Hide, De-hua LaiAbstract:Toxoplasma gondii can infect almost all warm-blooded vertebrates with pathogensis being largely influenced by the host immune status. As important epidemiological hosts, rodents are globally distributed and are also commonly found infected with haemoflagellates, such as those in the genus Trypanosoma. We here address whether and how co-infection with trypanosomes can influence T. gondii infection in laboratory models. Rats of five strains, co-infected with T. Lewisi and mice of four strains, co-infected with T. musculi, were found to be more or less susceptible to T. gondii infection, respectively, with corresponding increased or decreased brain cyst burdens. Downregulation of iNOS expression and decreased NO production or reverse were observed in the peritoneal macrophages of rats or mice, infected with trypanosomes, respectively. Trypanosoma Lewisi and T. musculi can modulate host immune responses, either by enhancement or suppression and influence the outcome of Toxoplasma infection.
-
Novel organization of mitochondrial minicircles and guide RNAs in the zoonotic pathogen Trypanosoma Lewisi.
Nucleic acids research, 2020Co-Authors: Xuan Zhang, Geoff Hide, De-hua Lai, Julius Lukeš, Ju-feng Wang, Zhao-rong LunAbstract:Abstract Kinetoplastid flagellates are known for several unusual features, one of which is their complex mitochondrial genome, known as kinetoplast (k) DNA, composed of mutually catenated maxi- and minicircles. Trypanosoma Lewisi is a member of the Stercorarian group of trypanosomes which is, based on human infections and experimental data, now considered a zoonotic pathogen. By assembling a total of 58 minicircle classes, which fall into two distinct categories, we describe a novel type of kDNA organization in T. Lewisi. RNA-seq approaches allowed us to map the details of uridine insertion and deletion editing events upon the kDNA transcriptome. Moreover, sequencing of small RNA molecules enabled the identification of 169 unique guide (g) RNA genes, with two differently organized minicircle categories both encoding essential gRNAs. The unprecedented organization of minicircles and gRNAs in T. Lewisi broadens our knowledge of the structure and expression of the mitochondrial genomes of these human and animal pathogens. Finally, a scenario describing the evolution of minicircles is presented.
-
Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma Lewisi, a zoonotic pathogen.
Cell cycle (Georgetown Tex.), 2019Co-Authors: Xuan Zhang, Geoff Hide, De-hua Lai, Zhao-rong LunAbstract:Trypanosoma (Herpetosoma) Lewisi is a globally distributed rat trypanosome, currently considered as a zoonotic pathogen; however, a detailed understanding of the morphological events occurring during the cell cycle is lacking. This study aimed to investigate the cell cycle morphology and cleavage events of Trypanosoma Lewisi (T. Lewisi) during in vitro cultivation. By establishing in vitro cultivation of T. Lewisi at 37°C, various cell morphologies and stages could be observed. We have provided a quantitative analysis of the morphological events during T. Lewisi proliferation. We confirmed a generation time of 12.14 ± 0.79 hours, which is similar to that in vivo (12.21 ± 0.14 hours). We also found that there are two distinct cell cycles, with a two-way transformation connection in the developmental status of this parasite, which was contrasted with the previous model of multiple division patterns seen in T. Lewisi. We quantified the timing of cell cycle phases (G1n, 0.56 U; Sn, 0.14 U; G2n, 0.16 U; M, 0.06 U; C, 0.08 U; G1k, 0.65 U; Sk, 0.10 U; G2k, 0.17 U; D, 0.03 U; A, 0.05 U) and their morphological characteristics, particularly with respect to the position of kinetoplast(s) and nucleus/nuclei. Interestingly, we found that nuclear replication in T. Lewisi occurred prior to kinetoplast replication, different to the order of replication found in Trypanosoma brucei and Trypanosoma cruzi, implicating a distinct cell cycle control mechanism in T. Lewisi. We characterized the morphological events during the T. Lewisi cell cycle and presented evidence to support the existence of two distinct cell cycles with two-way transformation between them. These results provide insights into the differentiation and evolution of this parasite and its related species. Keywords: Trypanosoma Lewisi, Cell cycle, In vitro, Multiplication, Division, Zoonotic pathogen
Jiang-mei Gao - One of the best experts on this subject based on the ideXlab platform.
-
Infection with Trypanosoma Lewisi or Trypanosoma musculi may promote the spread of Toxoplasma gondii.
Parasitology, 2021Co-Authors: Jiang-mei Gao, Zhao-rong Lun, Guo-qing Geng, Geoff Hide, De-hua LaiAbstract:Toxoplasma gondii can infect almost all warm-blooded vertebrates with pathogensis being largely influenced by the host immune status. As important epidemiological hosts, rodents are globally distributed and are also commonly found infected with haemoflagellates, such as those in the genus Trypanosoma. We here address whether and how co-infection with trypanosomes can influence T. gondii infection in laboratory models. Rats of five strains, co-infected with T. Lewisi and mice of four strains, co-infected with T. musculi, were found to be more or less susceptible to T. gondii infection, respectively, with corresponding increased or decreased brain cyst burdens. Downregulation of iNOS expression and decreased NO production or reverse were observed in the peritoneal macrophages of rats or mice, infected with trypanosomes, respectively. Trypanosoma Lewisi and T. musculi can modulate host immune responses, either by enhancement or suppression and influence the outcome of Toxoplasma infection.
-
A preliminary serogical study of Trypanosoma evansi and Trypanosoma Lewisi
2018Co-Authors: Jiang-mei Gao, Truc Philippe, Desquenes M., Vincendeau P., Courtois P., Xuan Zhang, Jittapalapong S., Zhao-rong LunAbstract:Trypanosoma evansi, known as an animal trypanosome, is widely distributed in many countries of Africa, Asia and South America; it causes significant economic loss in these countries. A few cases have also occurred in some countries of Europe due to the importation of infected animals from endemic regions. Rare human T. evansi infections were attended by the health departments and international health organizations in these endemic countries. Trypanosoma Lewisi, a cosmopolitan parasite of rats, sometimes found in humans, is currently considered as a zoonotic pathogen and has gained special attention from scientists and international health organizations such as the World Health Organization. The current study considered the serological screening of human infection by T. evansi and T. Lewisi in a Chinese human population. None of the 622 samples was found positive for T. evansi infection using the card agglutination test for the trypanosome antigen Rotat 1.2, while, 2.41% of the examined serum samples exhibited some seropositivity to T. Lewisi using enzyme-linked immunosorbent assay. No significant difference was found between the samples from areas in the South (Zhaoqing, Guangdong) and Central (Zhengzhou, Henan) China
Clarence M. Lee - One of the best experts on this subject based on the ideXlab platform.
-
Comparative positions of kinetoplasts in Trypanosoma musculi and Trypanosoma Lewisi during development in vitro
Cell Proliferation, 2002Co-Authors: M. Ashraf, R. A. Nesbitt, P. A. Humphrey, M. Siewe, Clarence M. LeeAbstract:The development of Trypanosoma musculi and Trypanosoma Lewisi were studied in vitro in the presence of adherent splenic cells. Both parasites developed only when attached by their flagellar tips to adherent splenic cells. During the proliferation of T. musculi, the kinetoplast migrated towards the nucleus, and once in the vicinity of the nucleus, the nuclear division was triggered. The kinetoplast of T. Lewisi did not migrate towards the nucleus, but remained at its original location. The nucleus and kinetoplast divided at the same time in both parasites, and parasites started dividing from their flagellar ends and T. musculi and T. Lewisi daughter cells were formed within 48 h. The unavailability of the adherent splenic cells in vitro led the parasites to transform into round/oval nonviable forms.
