The Experts below are selected from a list of 3669 Experts worldwide ranked by ideXlab platform
Catherine W. Donnelly - One of the best experts on this subject based on the ideXlab platform.
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Increased detection of acid-injured Escherichia coli O157:H7 in autoclaved apple cider by using nonselective repair on Trypticase Soy Agar
Journal of Food Protection, 1997Co-Authors: Todd M. Silk, Catherine W. DonnellyAbstract:Three different acid-resistant strains of Escherichia coli O157:H7 were inoculated individually and as a cocktail into sterile apple cider (pH 3.2) at a level of approximately 105 cells per ml and incubated at 2°C. Samples were plated on Trypticase Soy Agar (TSA), violet red bile Agar (VRBA), sorbitol MacConkey Agar (SMA), and Petrifilm E. coli count plates (Petrifilm) at 24-h intervals. Repair of acid-injured cells was assessed by surface plating cider samples on TSA and allowing a 2-h room-temperature incubation period followed by overlaying with double-strength VRBA or SMA. Since SMA is a surface plate medium, the repair procedure was modified by overlaying SMA with Trypticase Soy broth after 2 h of room-temperature incubation. Populations of all three strains and the cocktail of strains decreased rapidly in apple cider and approached undetectable levels within 72 h. At 24 and 48 h, 98.4% and >99% of the E. coli populations were injured, respectively. Repair procedures significantly (α = 0.05) increase...
Mark A Miller - One of the best experts on this subject based on the ideXlab platform.
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optochin revisited defining the optimal type of blood Agar for presumptive identification of streptococcus pneumoniae
Journal of Clinical Microbiology, 1998Co-Authors: Michael Gardam, Mark A MillerAbstract:To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia Agar, Trypticase Soy Agar (TSA), and Mueller-Hinton Agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood Agar be used.
Todd M. Silk - One of the best experts on this subject based on the ideXlab platform.
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Increased detection of acid-injured Escherichia coli O157:H7 in autoclaved apple cider by using nonselective repair on Trypticase Soy Agar
Journal of Food Protection, 1997Co-Authors: Todd M. Silk, Catherine W. DonnellyAbstract:Three different acid-resistant strains of Escherichia coli O157:H7 were inoculated individually and as a cocktail into sterile apple cider (pH 3.2) at a level of approximately 105 cells per ml and incubated at 2°C. Samples were plated on Trypticase Soy Agar (TSA), violet red bile Agar (VRBA), sorbitol MacConkey Agar (SMA), and Petrifilm E. coli count plates (Petrifilm) at 24-h intervals. Repair of acid-injured cells was assessed by surface plating cider samples on TSA and allowing a 2-h room-temperature incubation period followed by overlaying with double-strength VRBA or SMA. Since SMA is a surface plate medium, the repair procedure was modified by overlaying SMA with Trypticase Soy broth after 2 h of room-temperature incubation. Populations of all three strains and the cocktail of strains decreased rapidly in apple cider and approached undetectable levels within 72 h. At 24 and 48 h, 98.4% and >99% of the E. coli populations were injured, respectively. Repair procedures significantly (α = 0.05) increase...
Michael Gardam - One of the best experts on this subject based on the ideXlab platform.
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optochin revisited defining the optimal type of blood Agar for presumptive identification of streptococcus pneumoniae
Journal of Clinical Microbiology, 1998Co-Authors: Michael Gardam, Mark A MillerAbstract:To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia Agar, Trypticase Soy Agar (TSA), and Mueller-Hinton Agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood Agar be used.
Tomoyuki Fujii - One of the best experts on this subject based on the ideXlab platform.
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Carbon Dioxide Inhibition of Escherichia coli and Staphylococcus aureus on a pH-adjusted Surface in a Model System
Journal of Food Science, 1999Co-Authors: Bon Kimura, T. Yoshiyama, Tomoyuki FujiiAbstract:Growth of Escherichia coli and Staphylococcus aureus on the surface of Trypticase Soy Agar (TSA) packaged with various CO 2 partial pressures (0, 20, 40, 60, 80, 100%, balance N 2 ) was compared to the control (N 2 100%) on TSA in which the pH was adjusted to equal that in CO 2 atmospheres at 15°C and 30°C. At 15°C, the biostatic effect was noted with all CO 2 partial pressures for both species. At 30°C, the biostatic effect of CO 2 was almost completely nullified for E. coli, but that for S. aureus was still effective. S. aureus was more sensitive to the inhibitory effects of CO 2 than E. coli at both the temperatures.
