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Patel Bharat - One of the best experts on this subject based on the ideXlab platform.
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Caloramator mitchellensis sp. nov., a thermoanaerobe isolated from the geothermal waters of the Great Artesian Basin of Australia, and emended description of the genus Caloramator
Society for General Microbiology, 2011Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly thermophilic anaerobe, designated strain VF08T, was isolated from a water sample collected from a Great Artesian Basin bore (registered bore number 22981) situated at Mitchell, QLD, Australia. Cells of isolate VF08T were slightly curved, non-sporulating rods (1.5-3.5װ.4-0.8 孩, which stained Gram-negative but possessed a Gram-positive cell-wall ultrastructure. The strain grew optimally in Tryptone-yeast extract-glucose (TYEG) medium at 55 à(temperature growth range between 37 and 60 é and a pH of 7 (pH growth range, 6.0-9.0). Yeast extract or Tryptone was required for growth on glucose, fructose, xylose, maltose, sucrose, raffinose, cellobiose, ribose, pyruvate, Tryptone, peptone, Casamino acids, amyl media and serine, but could also support growth as the sole carbon source. End products from glucose fermentation were acetate, ethanol, CO2 and H2. The strain reduced vanadium(V), but not iron(III), manganese(IV), elemental sulfur, sulfate, thiosulfate, sulfite, nitrate or nitrite in the presence of 0.2?% yeast extract, peptone, Tryptone, glucose, sucrose and Casamino acids, but an increase in the growth rate or cell yield was not observed. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and =2?% NaCl (w/v). The G+C content of the DNA was 38.4ᰮ8 mol% as determined by the thermal denaturation (Tm) method. 16S rRNA gene sequence analysis revealed that isolate VF08T was a member of the genus Caloramator with Caloramator australicus and Caloramator fervidus (formerly Clostridium fervidus) being the closest relatives with similarity values of 85.0 and 86.1?%, respectively, when helix 6 nucleotides were included in the analysis, and 95.2?% and 94?%, respectively, when these nucleotides were masked from the analysis. Further analysis revealed that strain VF08T formed an individual cluster (cluster II) within the genus Caloramator and could be distinguished from other species within the genus Caloramator (clusters I, III and IV) on the basis of signature nucleotides and differences in phenotypic traits. These data suggest that strain VF08T is a novel species of the genus Caloramator, for which the name Caloramator mitchellensis sp. nov. is proposed. The type strain is VF08T (=JCM 15828T=KCTC 5735T). An emended description of the genus Caloramator is also provided.No Full Tex
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Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters
'Microbiology Society', 2010Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly anaerobic, thermophilic bacterium, designated strain AeBT, was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeBT were slightly curved rods (2.5–6.0×1.0 μm) that stained Gram-negative and formed spherical terminal to subterminal spores. The strain grew optimally in Tryptone–yeast extract–Casamino acids medium at 50 °C (range 37–55 °C) and pH 7 (range pH 5–9). Strain AeBT grew poorly on yeast extract (0.2 %) and Tryptone (0.2 %) as sole carbon sources, which were obligately required for growth on other energy sources. Growth of strain AeBT increased in the presence of various carbohydrates and amino acids, but not organic acids. End products detected from glucose fermentation were ethanol, acetate, CO2 and H2. In the presence of 0.2 % yeast extract, iron(III), manganese(IV), vanadium(V) and cobalt(III) were reduced, but not sulfate, thiosulfate, sulfite, elemental sulfur, nitrate or nitrite. Iron(III) was also reduced in the presence of Tryptone, peptone, Casamino acids and amyl media (Research Achievement), but not starch, xylan, chitin, glycerol, ethanol, pyruvate, benzoate, lactate, acetate, propionate, succinate, glycine, serine, lysine, threonine, arginine, glutamate, valine, leucine, histidine, alanine, aspartate, isoleucine or methionine. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and NaCl concentrations >2 %. The DNA G+C content was 35.4±1 mol%, as determined by the thermal denaturation method. 16S rRNA gene sequence analysis indicated that strain AeBT is a member of the family Clostridiaceae, class Clostridia, phylum ‘Firmicutes’, and is positioned approximately equidistantly between the genera Sarcina, Anaerobacter, Caloramator and Clostridium (16S rRNA gene similarity values of 87.8–90.9 %). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain AeBT is considered to represent a novel species in a new genus, for which the name Fervidicella metallireducens gen. nov., sp. nov. is proposed; the type strain is AeBT (=JCM 15555T=KCTC 5667T)
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Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters
Society for General Microbiology, 2010Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly anaerobic, thermophilic bacterium, designated strain AeBT, was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeBT were slightly curved rods (2.5-6.0x1.0 孩 that stained Gram-negative and formed spherical terminal to subterminal spores. The strain grew optimally in Tryptone-yeast extract-Casamino acids medium at 50 à(range 37-55 é and pH 7 (range pH 5-9). Strain AeBT grew poorly on yeast extract (0.2 %) and Tryptone (0.2 %) as sole carbon sources, which were obligately required for growth on other energy sources. Growth of strain AeBT increased in the presence of various carbohydrates and amino acids, but not organic acids. End products detected from glucose fermentation were ethanol, acetate, CO2 and H2. In the presence of 0.2 % yeast extract, iron(III), manganese(IV), vanadium(V) and cobalt(III) were reduced, but not sulfate, thiosulfate, sulfite, elemental sulfur, nitrate or nitrite. Iron(III) was also reduced in the presence of Tryptone, peptone, Casamino acids and amyl media (Research Achievement), but not starch, xylan, chitin, glycerol, ethanol, pyruvate, benzoate, lactate, acetate, propionate, succinate, glycine, serine, lysine, threonine, arginine, glutamate, valine, leucine, histidine, alanine, aspartate, isoleucine or methionine. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and NaCl concentrations >2 %. The DNA G+C content was 35.4ᱠmol%, as determined by the thermal denaturation method. 16S rRNA gene sequence analysis indicated that strain AeBT is a member of the family Clostridiaceae, class Clostridia, phylum 'Firmicutes', and is positioned approximately equidistantly between the genera Sarcina, Anaerobacter, Caloramator and Clostridium (16S rRNA gene similarity values of 87.8-90.9 %). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain AeBT is considered to represent a novel species in a new genus, for which the name Fervidicella metallireducens gen. nov., sp. nov. is proposed; the type strain is AeBT (=JCM 15555T=KCTC 5667T).No Full Tex
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Fervidicola ferrireducens gen. nov., sp. nov., a thermophilic anaerobic bacterium from geothermal waters of the Great Artesian Basin, Australia
Society for General Microbiology, 2009Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly anaerobic, thermophilic bacterium, designated strain Y170T, was isolated from a microbial mat colonizing thermal waters of a run-off channel created by the free-flowing waters of a Great Artesian Basin (GAB) bore well (New Lorne bore; registered number 17263). Cells of strain Y170T were slightly curved rods (1.2-12 0.8-1.1 mm) and stained Gram-negative. The strain grew optimally in Tryptone-yeast extract-glucose medium at 70 6C (temperature range for growth was 55-80 6C) and pH 7 (pH range for growth was 5-9). Strain Y170T grew poorly on yeast extract as a sole carbon source, but not on Tryptone (0.2 %). Yeast extract could not be replaced by Tryptone and was obligately required for growth on Tryptone, peptone, glucose, fructose, galactose, cellobiose, mannose, sucrose, xylose, mannitol, formate, pyruvate, Casamino acids and threonine. No growth was observed on arabinose, lactose, maltose, raffinose, chitin, xylan, pectin, starch, acetate, benzoate, lactate, propionate, succinate, myo-inositol, ethanol, glycerol, amyl media, aspartate, leucine, glutamate, alanine, arginine, serine and glycine. End products detected from glucose fermentation were acetate, ethanol and presumably CO2 and H2. Iron(III), manganese(IV), thiosulfate and elemental sulfur, but not sulfate, sulfite, nitrate or nitrite, were used as electron acceptors in the presence of 0.2% yeast extract. Iron(III) in the form of amorphous Fe(III) oxhydroxide and Fe(III) citrate was also reduced in the presence of Tryptone, peptone and Casamino acids, but not with chitin, xylan, pectin, formate, starch, pyruvate, acetate, benzoate, threonine, lactate, propionate, succinate, inositol, ethanol, glycerol, mannitol, aspartate, leucine, glutamate, alanine, arginine, serine or glycine. Strain Y170T was not able to utilize molecular hydrogen and/or carbon dioxide in the presence or absence of iron(III). Chloramphenicol, streptomycin, tetracycline, penicillin and ampicillin and NaCl concentrations greater than 2% inhibited growth. The G+C content of the DNA was 48ᱠmol% [SD (n53); Tm]. 16S rRNA gene sequence analysis indicated that strain Y170T is a member of the family Syntrophomonadaceae, class Clostridia, phylum Firmicutes and was most closely related to members of the genus Thermosediminibacter (mean similarity of 93.6 %). On the basis of the 16S rRNA gene sequence comparisons and physiological characteristics, strain Y170T is considered to represent a novel species of a new genus, for which the name Fervidicola ferrireducens gen. nov., sp. nov. is proposed. The type strain is Y170T (5KCTC 5610T5JCM 15106T5DSM 21121T).No Full Tex
Namudar İzzet Kurbanoğlu - One of the best experts on this subject based on the ideXlab platform.
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Utilization as peptone for glycerol production of ram horn waste with a new process
Energy Conversion and Management, 2004Co-Authors: Esabi Basaran Kurbanoglu, Namudar İzzet KurbanoğluAbstract:Abstract A major component of the horns is protein. Peptones are defined as protein hydrolysates. The potential use of ram horn peptone (RHP) as a nitrogen source for glycerol production by Saccharomyces cerevisiae was studied. For this purpose, first, RHP was produced. Ram horns were hydrolyzed by treating with acids (6 N H 2 SO 4 and 6 N HCl) and neutralizing the solutions. The amounts of protein, nitrogen, ash, some minerals, total sugars, total lipids and amino acids of the RHP were determined. The RHP was compared with a bacto-Tryptone from casein and other peptones. With the addition of RHP to the fermentation medium with a final concentration of 4% (optimal concentration), the glycerol value for 4 days reached a maximum value (8.5 g l −1 ), which is 25% higher than that of the bacto-Tryptone (6.8 g l −1 ), 32% higher than that of bacto-peptone (6.4 g l −1 ) and 49% higher than that of fish peptone (5.7 g l −1 ). The results show that RHP can be utilized as a peptone and may be a valuable supplement in biotechnology.
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A new process for the utilization as peptone of ram horn waste
Journal of Bioscience and Bioengineering, 2002Co-Authors: Esabi Basaran Kurbanoglu, Namudar İzzet KurbanoğluAbstract:A peptone for utilization in microbiological culture media can be produced from rams horns. A major component of the horns is protein. Peptones are defined as protein hydrolysates. Ram horns obtained from a slaughterhouse in Erzurum, Turkey were hydrolyzed by treatment with acid (6N-HCl) and ram horn hydrolysate (RHH) was obtained. The RHH was evaporated and termed ram horn peptone (RHP). RHP was compared with a bacto-Tryptone from casein and other peptones. The results show that RHP can be utilized as a peptone and may be a valuable supplement in biotechnology.
Bharat K C Patel - One of the best experts on this subject based on the ideXlab platform.
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caloramator australicus sp nov a thermophilic anaerobic bacterium from the great artesian basin of australia
International Journal of Systematic and Evolutionary Microbiology, 2009Co-Authors: Christopher David Ogg, Bharat K C PatelAbstract:A strictly anaerobic, thermophilic bacterium, designated strain RC3T, was isolated from microbial mats colonizing thermal waters of a run-off channel formed by free-flowing waters from a bore well (registered no. 17263) of the Great Artesian Basin, Australia. The slightly curved rods (2.5-4.2x0.8-1.0 microm) of strain RC3T stained Gram-positive and grew optimally in Tryptone-yeast extract-glucose medium at 60 degrees C (range 45-70 degrees C) and pH 7 (range pH 5-9). Strain RC3T grew poorly on yeast extract (0.2 %) but did not grow on Tryptone (0.2 %) as a sole carbon source; yeast extract was required for growth on other energy sources, which included glucose, fructose, galactose, xylose, maltose, sucrose, raffinose, mannose, cellobiose, cellulose, starch, amylopectin, xylan, peptone, amyl media (Research Achievement), threonine and pyruvate but did not include arabinose, ribose, lactose, CM-cellulose, myo-inositol, mannitol, chitin, casein, formate, acetate, succinate, propionate, lactate, benzoate, glycerol, ethanol, Casamino acids, arginine, alanine, serine, glycine, glutamine, leucine, isoleucine, methionine or aspartate. The end products of glucose fermentation were ethanol and acetate. In the presence of 0.2 % yeast extract, iron(III), manganese(IV) and elemental sulfur were reduced but not sulfate, sulfite, thiosulfate, nitrate or nitrite. Iron(III) was also reduced in the presence of peptone, Tryptone, amyl media, threonine and glycerol but not chitin, xylan, pectin, starch, pyruvate, acetate, benzoate, lactate, propionate, succinate, inositol, ethanol, mannitol, arginine, glutamine or serine. Strain RC3T was not able to utilize molecular hydrogen and/or carbon dioxide in the presence or absence of iron(III). In the presence of iron(III) and glycerol, increased concentrations of Fe(II) corresponded to increased cell numbers, demonstrating that strain RC3(T) was able to conserve energy to support growth from the reduction of Fe(III) to Fe(II). Chloramphenicol, streptomycin, tetracycline, penicillin and ampicillin and NaCl concentrations greater than 2 % inhibited growth. The G+C content of the DNA was 34+/-1 mol% as determined by the thermal denaturation (Tm) method. 16S rRNA gene sequence analysis indicated that strain RC3T was affiliated to Caloramator fervidus (95.8 % similarity to the type strain) and to other Caloramator species (average similarity of 91.6 %) within the phylum Firmicutes. On the basis of phylogenetic and phenotypic characteristics, it is proposed that strain RC3T should be classified in the genus Caloramator as a representative of a novel species, Caloramator australicus sp. nov. The type strain is RC3T (=JCM 1508T =KCTC 5601T).
Ogg Christopher - One of the best experts on this subject based on the ideXlab platform.
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Caloramator mitchellensis sp. nov., a thermoanaerobe isolated from the geothermal waters of the Great Artesian Basin of Australia, and emended description of the genus Caloramator
Society for General Microbiology, 2011Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly thermophilic anaerobe, designated strain VF08T, was isolated from a water sample collected from a Great Artesian Basin bore (registered bore number 22981) situated at Mitchell, QLD, Australia. Cells of isolate VF08T were slightly curved, non-sporulating rods (1.5-3.5װ.4-0.8 孩, which stained Gram-negative but possessed a Gram-positive cell-wall ultrastructure. The strain grew optimally in Tryptone-yeast extract-glucose (TYEG) medium at 55 à(temperature growth range between 37 and 60 é and a pH of 7 (pH growth range, 6.0-9.0). Yeast extract or Tryptone was required for growth on glucose, fructose, xylose, maltose, sucrose, raffinose, cellobiose, ribose, pyruvate, Tryptone, peptone, Casamino acids, amyl media and serine, but could also support growth as the sole carbon source. End products from glucose fermentation were acetate, ethanol, CO2 and H2. The strain reduced vanadium(V), but not iron(III), manganese(IV), elemental sulfur, sulfate, thiosulfate, sulfite, nitrate or nitrite in the presence of 0.2?% yeast extract, peptone, Tryptone, glucose, sucrose and Casamino acids, but an increase in the growth rate or cell yield was not observed. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and =2?% NaCl (w/v). The G+C content of the DNA was 38.4ᰮ8 mol% as determined by the thermal denaturation (Tm) method. 16S rRNA gene sequence analysis revealed that isolate VF08T was a member of the genus Caloramator with Caloramator australicus and Caloramator fervidus (formerly Clostridium fervidus) being the closest relatives with similarity values of 85.0 and 86.1?%, respectively, when helix 6 nucleotides were included in the analysis, and 95.2?% and 94?%, respectively, when these nucleotides were masked from the analysis. Further analysis revealed that strain VF08T formed an individual cluster (cluster II) within the genus Caloramator and could be distinguished from other species within the genus Caloramator (clusters I, III and IV) on the basis of signature nucleotides and differences in phenotypic traits. These data suggest that strain VF08T is a novel species of the genus Caloramator, for which the name Caloramator mitchellensis sp. nov. is proposed. The type strain is VF08T (=JCM 15828T=KCTC 5735T). An emended description of the genus Caloramator is also provided.No Full Tex
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Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters
'Microbiology Society', 2010Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly anaerobic, thermophilic bacterium, designated strain AeBT, was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeBT were slightly curved rods (2.5–6.0×1.0 μm) that stained Gram-negative and formed spherical terminal to subterminal spores. The strain grew optimally in Tryptone–yeast extract–Casamino acids medium at 50 °C (range 37–55 °C) and pH 7 (range pH 5–9). Strain AeBT grew poorly on yeast extract (0.2 %) and Tryptone (0.2 %) as sole carbon sources, which were obligately required for growth on other energy sources. Growth of strain AeBT increased in the presence of various carbohydrates and amino acids, but not organic acids. End products detected from glucose fermentation were ethanol, acetate, CO2 and H2. In the presence of 0.2 % yeast extract, iron(III), manganese(IV), vanadium(V) and cobalt(III) were reduced, but not sulfate, thiosulfate, sulfite, elemental sulfur, nitrate or nitrite. Iron(III) was also reduced in the presence of Tryptone, peptone, Casamino acids and amyl media (Research Achievement), but not starch, xylan, chitin, glycerol, ethanol, pyruvate, benzoate, lactate, acetate, propionate, succinate, glycine, serine, lysine, threonine, arginine, glutamate, valine, leucine, histidine, alanine, aspartate, isoleucine or methionine. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and NaCl concentrations >2 %. The DNA G+C content was 35.4±1 mol%, as determined by the thermal denaturation method. 16S rRNA gene sequence analysis indicated that strain AeBT is a member of the family Clostridiaceae, class Clostridia, phylum ‘Firmicutes’, and is positioned approximately equidistantly between the genera Sarcina, Anaerobacter, Caloramator and Clostridium (16S rRNA gene similarity values of 87.8–90.9 %). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain AeBT is considered to represent a novel species in a new genus, for which the name Fervidicella metallireducens gen. nov., sp. nov. is proposed; the type strain is AeBT (=JCM 15555T=KCTC 5667T)
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Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters
Society for General Microbiology, 2010Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly anaerobic, thermophilic bacterium, designated strain AeBT, was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeBT were slightly curved rods (2.5-6.0x1.0 孩 that stained Gram-negative and formed spherical terminal to subterminal spores. The strain grew optimally in Tryptone-yeast extract-Casamino acids medium at 50 à(range 37-55 é and pH 7 (range pH 5-9). Strain AeBT grew poorly on yeast extract (0.2 %) and Tryptone (0.2 %) as sole carbon sources, which were obligately required for growth on other energy sources. Growth of strain AeBT increased in the presence of various carbohydrates and amino acids, but not organic acids. End products detected from glucose fermentation were ethanol, acetate, CO2 and H2. In the presence of 0.2 % yeast extract, iron(III), manganese(IV), vanadium(V) and cobalt(III) were reduced, but not sulfate, thiosulfate, sulfite, elemental sulfur, nitrate or nitrite. Iron(III) was also reduced in the presence of Tryptone, peptone, Casamino acids and amyl media (Research Achievement), but not starch, xylan, chitin, glycerol, ethanol, pyruvate, benzoate, lactate, acetate, propionate, succinate, glycine, serine, lysine, threonine, arginine, glutamate, valine, leucine, histidine, alanine, aspartate, isoleucine or methionine. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and NaCl concentrations >2 %. The DNA G+C content was 35.4ᱠmol%, as determined by the thermal denaturation method. 16S rRNA gene sequence analysis indicated that strain AeBT is a member of the family Clostridiaceae, class Clostridia, phylum 'Firmicutes', and is positioned approximately equidistantly between the genera Sarcina, Anaerobacter, Caloramator and Clostridium (16S rRNA gene similarity values of 87.8-90.9 %). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain AeBT is considered to represent a novel species in a new genus, for which the name Fervidicella metallireducens gen. nov., sp. nov. is proposed; the type strain is AeBT (=JCM 15555T=KCTC 5667T).No Full Tex
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Fervidicola ferrireducens gen. nov., sp. nov., a thermophilic anaerobic bacterium from geothermal waters of the Great Artesian Basin, Australia
Society for General Microbiology, 2009Co-Authors: Ogg Christopher, Patel BharatAbstract:A strictly anaerobic, thermophilic bacterium, designated strain Y170T, was isolated from a microbial mat colonizing thermal waters of a run-off channel created by the free-flowing waters of a Great Artesian Basin (GAB) bore well (New Lorne bore; registered number 17263). Cells of strain Y170T were slightly curved rods (1.2-12 0.8-1.1 mm) and stained Gram-negative. The strain grew optimally in Tryptone-yeast extract-glucose medium at 70 6C (temperature range for growth was 55-80 6C) and pH 7 (pH range for growth was 5-9). Strain Y170T grew poorly on yeast extract as a sole carbon source, but not on Tryptone (0.2 %). Yeast extract could not be replaced by Tryptone and was obligately required for growth on Tryptone, peptone, glucose, fructose, galactose, cellobiose, mannose, sucrose, xylose, mannitol, formate, pyruvate, Casamino acids and threonine. No growth was observed on arabinose, lactose, maltose, raffinose, chitin, xylan, pectin, starch, acetate, benzoate, lactate, propionate, succinate, myo-inositol, ethanol, glycerol, amyl media, aspartate, leucine, glutamate, alanine, arginine, serine and glycine. End products detected from glucose fermentation were acetate, ethanol and presumably CO2 and H2. Iron(III), manganese(IV), thiosulfate and elemental sulfur, but not sulfate, sulfite, nitrate or nitrite, were used as electron acceptors in the presence of 0.2% yeast extract. Iron(III) in the form of amorphous Fe(III) oxhydroxide and Fe(III) citrate was also reduced in the presence of Tryptone, peptone and Casamino acids, but not with chitin, xylan, pectin, formate, starch, pyruvate, acetate, benzoate, threonine, lactate, propionate, succinate, inositol, ethanol, glycerol, mannitol, aspartate, leucine, glutamate, alanine, arginine, serine or glycine. Strain Y170T was not able to utilize molecular hydrogen and/or carbon dioxide in the presence or absence of iron(III). Chloramphenicol, streptomycin, tetracycline, penicillin and ampicillin and NaCl concentrations greater than 2% inhibited growth. The G+C content of the DNA was 48ᱠmol% [SD (n53); Tm]. 16S rRNA gene sequence analysis indicated that strain Y170T is a member of the family Syntrophomonadaceae, class Clostridia, phylum Firmicutes and was most closely related to members of the genus Thermosediminibacter (mean similarity of 93.6 %). On the basis of the 16S rRNA gene sequence comparisons and physiological characteristics, strain Y170T is considered to represent a novel species of a new genus, for which the name Fervidicola ferrireducens gen. nov., sp. nov. is proposed. The type strain is Y170T (5KCTC 5610T5JCM 15106T5DSM 21121T).No Full Tex
Manu Lopus - One of the best experts on this subject based on the ideXlab platform.
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Tryptone-stabilized gold nanoparticles induce unipolar clustering of supernumerary centrosomes and G1 arrest in triple-negative breast cancer cells.
Scientific reports, 2019Co-Authors: J. Grace Nirmala, Manu LopusAbstract:Gold nanoparticles of different sizes, shapes, and decorations exert a variety of effects on biological systems. We report a novel mechanism of action of chemically modified, Tryptone-stabilized gold nanoparticles (T-GNPs) in the triple-negative breast cancer (TNBC) cell line, MDA-MB-231. The T-GNPs, synthesized using HAuCl4.3H2O and Tryptone and characterized by an assortment of spectroscopy techniques combined with high-resolution electron microscopy, demonstrated strong antiproliferative and anti-clonogenic potential against MDA-MB-231 cells, arresting them at the G1 phase of the cell cycle and promoting apoptosis. The molecular mechanism of action of these particles involved induction of unipolar clustering and hyper amplification of the supernumerary centrosomes (a distinctive feature of many tumour cells, including TNBC cells). The clustering was facilitated by microtubules with suppressed dynamicity. Mass spectrometry-assisted proteomic analysis revealed that the T-GNP-induced G1 arrest was facilitated, at least in part, by downregulation of ribosome biogenesis pathways. Due to the presence of supernumerary centrosomes in many types of tumour cells, we propose chemical induction of their unipolar clustering as a potential therapeutic strategy.
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Tryptone-stabilized gold nanoparticles target tubulin and inhibit cell viability by inducing an unusual form of cell cycle arrest
Experimental cell research, 2017Co-Authors: Tejashree Mahaddalkar, Sourabh Mehta, Sanith Cheriyamundath, H. Muthurajan, Manu LopusAbstract:Gold nanoparticles have been investigated extensively for their molecular mechanisms of action and anticancer potential. We report a novel, tubulin-targeted antiproliferative mechanism of action of Tryptone-stabilized gold nanoparticles (TsAuNPs). TsAuNPs, synthesized using HAuCl4·3H2O and Tryptone and characterized by a variety of spectroscopic methods and transmission electron microscopy, were found to be inhibitory to viability of human pancreatic (PANC-1), cervical (HeLa), and breast (MDA-MB-231) cancer cell lines in a concentration-dependent manner, with highest efficacy against PANC-1 cells. The particles strongly inhibited the clonogenic propagation of PANC-1 cells. TsAuNPs-mediated inhibition of cell viability involved an unusual mode of cell cycle arrest (arrest at both G0/G1 phase and S-phase) followed by apoptosis. In vitro, TsAuNPs bound purified tubulin, competitively inhibited anilinonaphthalene sulfonate binding to tubulin, and suppressed tubulin assembly. In cells, tubulin-TsAuNPs interactions were manifested as a disrupted microtubule network, defective reassembly of cold-disassembled microtubules, and induction of tubulin acetylation. Our data indicate that TsAuNPs inhibit cell viability by inducing differential cell cycle arrest possibly through disrupted dynamicity of cellular microtubules.
