The Experts below are selected from a list of 42 Experts worldwide ranked by ideXlab platform
Antonietta Mello - One of the best experts on this subject based on the ideXlab platform.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brule, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brule of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brule, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brule contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brule. In addition, Fisher’s Exact Tests detected more biological processes inside the brule; these processes were related to responses to multiple types of stress. Thus, although the brule has a reduced diversity of plant and microbial species, the organisms in the brule show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific Reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brûlé, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brûlé of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brûlé, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brûlé contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brûlé. In addition, Fisher’s Exact Tests detected more biological processes inside the brûlé; these processes were related to responses to multiple types of stress. Thus, although the brûlé has a reduced diversity of plant and microbial species, the organisms in the brûlé show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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PCR primers specific for the Genus Tuber reveal the presence of several truffle species in a truffle-ground
FEMS microbiology letters, 2009Co-Authors: Elisa Zampieri, Antonietta Mello, Paola Bonfante, Claude MuratAbstract:Truffles are hypogeous Ascomycete fungi belonging to the Genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the Genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As Genus-specific primers are not available, the aims of this work were (1) to assess the usefulness of the β-tubulin gene as a DNA barcoding region for designing Tuber Genus-specific primers, (2) to test the primers on a range of fruiting bodies, representing a large part of truffle biodiversity and (3) to check their ecological usefulness, applying them to truffle-ground soil. The new primers designed on the β-tubulin gene were specific to the Tuber Genus in nested PCR. When applied to DNA from soils, they gave a positive signal for 23 of 32 soils. Phylogenetic analysis confirmed that the bands corresponded to Tuber and that at least five Tuber species were present in the truffle-ground. β-tubulin was found to be a good barcoding region for designing Tuber Genus-specific primers, detecting a high Tuber diversity in a natural environment. These primers will be useful for understanding truffle ecology and for practical needs in plantation management.
Elisa Zampieri - One of the best experts on this subject based on the ideXlab platform.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brule, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brule of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brule, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brule contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brule. In addition, Fisher’s Exact Tests detected more biological processes inside the brule; these processes were related to responses to multiple types of stress. Thus, although the brule has a reduced diversity of plant and microbial species, the organisms in the brule show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific Reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brûlé, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brûlé of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brûlé, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brûlé contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brûlé. In addition, Fisher’s Exact Tests detected more biological processes inside the brûlé; these processes were related to responses to multiple types of stress. Thus, although the brûlé has a reduced diversity of plant and microbial species, the organisms in the brûlé show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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PCR primers specific for the Genus Tuber reveal the presence of several truffle species in a truffle-ground
FEMS microbiology letters, 2009Co-Authors: Elisa Zampieri, Antonietta Mello, Paola Bonfante, Claude MuratAbstract:Truffles are hypogeous Ascomycete fungi belonging to the Genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the Genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As Genus-specific primers are not available, the aims of this work were (1) to assess the usefulness of the β-tubulin gene as a DNA barcoding region for designing Tuber Genus-specific primers, (2) to test the primers on a range of fruiting bodies, representing a large part of truffle biodiversity and (3) to check their ecological usefulness, applying them to truffle-ground soil. The new primers designed on the β-tubulin gene were specific to the Tuber Genus in nested PCR. When applied to DNA from soils, they gave a positive signal for 23 of 32 soils. Phylogenetic analysis confirmed that the bands corresponded to Tuber and that at least five Tuber species were present in the truffle-ground. β-tubulin was found to be a good barcoding region for designing Tuber Genus-specific primers, detecting a high Tuber diversity in a natural environment. These primers will be useful for understanding truffle ecology and for practical needs in plantation management.
Paola Bonfante - One of the best experts on this subject based on the ideXlab platform.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brule, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brule of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brule, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brule contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brule. In addition, Fisher’s Exact Tests detected more biological processes inside the brule; these processes were related to responses to multiple types of stress. Thus, although the brule has a reduced diversity of plant and microbial species, the organisms in the brule show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific Reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brûlé, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brûlé of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brûlé, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brûlé contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brûlé. In addition, Fisher’s Exact Tests detected more biological processes inside the brûlé; these processes were related to responses to multiple types of stress. Thus, although the brûlé has a reduced diversity of plant and microbial species, the organisms in the brûlé show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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PCR primers specific for the Genus Tuber reveal the presence of several truffle species in a truffle-ground
FEMS microbiology letters, 2009Co-Authors: Elisa Zampieri, Antonietta Mello, Paola Bonfante, Claude MuratAbstract:Truffles are hypogeous Ascomycete fungi belonging to the Genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the Genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As Genus-specific primers are not available, the aims of this work were (1) to assess the usefulness of the β-tubulin gene as a DNA barcoding region for designing Tuber Genus-specific primers, (2) to test the primers on a range of fruiting bodies, representing a large part of truffle biodiversity and (3) to check their ecological usefulness, applying them to truffle-ground soil. The new primers designed on the β-tubulin gene were specific to the Tuber Genus in nested PCR. When applied to DNA from soils, they gave a positive signal for 23 of 32 soils. Phylogenetic analysis confirmed that the bands corresponded to Tuber and that at least five Tuber species were present in the truffle-ground. β-tubulin was found to be a good barcoding region for designing Tuber Genus-specific primers, detecting a high Tuber diversity in a natural environment. These primers will be useful for understanding truffle ecology and for practical needs in plantation management.
Stefania Daghino - One of the best experts on this subject based on the ideXlab platform.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific Reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brûlé, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brûlé of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brûlé, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brûlé contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brûlé. In addition, Fisher’s Exact Tests detected more biological processes inside the brûlé; these processes were related to responses to multiple types of stress. Thus, although the brûlé has a reduced diversity of plant and microbial species, the organisms in the brûlé show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brule, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brule of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brule, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brule contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brule. In addition, Fisher’s Exact Tests detected more biological processes inside the brule; these processes were related to responses to multiple types of stress. Thus, although the brule has a reduced diversity of plant and microbial species, the organisms in the brule show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
Marco Chiapello - One of the best experts on this subject based on the ideXlab platform.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific Reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brûlé, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brûlé of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brûlé, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brûlé contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brûlé. In addition, Fisher’s Exact Tests detected more biological processes inside the brûlé; these processes were related to responses to multiple types of stress. Thus, although the brûlé has a reduced diversity of plant and microbial species, the organisms in the brûlé show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
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Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles
Scientific reports, 2016Co-Authors: Elisa Zampieri, Paola Bonfante, Marco Chiapello, Stefania Daghino, Antonietta MelloAbstract:For some truffle species of the Tuber Genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brule, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brule of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brule, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brule contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brule. In addition, Fisher’s Exact Tests detected more biological processes inside the brule; these processes were related to responses to multiple types of stress. Thus, although the brule has a reduced diversity of plant and microbial species, the organisms in the brule show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.
