The Experts below are selected from a list of 2661 Experts worldwide ranked by ideXlab platform
William P Schiemann - One of the best experts on this subject based on the ideXlab platform.
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sox4 emt programs and the metastatic progression of breast cancers mastering the masters of emt
Breast Cancer Research, 2013Co-Authors: Jenny G Parvani, William P SchiemannAbstract:Epithelial-mesenchymal transition (EMT) programs require the expression of a variety of so-called master regulators of EMT, including members of the Snail, Zeb, and Twist Transcription Factor families. Teleologically, the requirement for such a diverse group of ‘master regulators’ seems evolutionarily cumbersome, and emerging evidence indicates that these Transcription Factors do in fact mediate unique and specialized functions, suggesting the existence of higher-order ‘masters’ that truly direct and coordinate EMT programs. Accordingly, Tiwari and colleagues recently delineated an elegant pathway wherein transforming growth Factor-beta stimulates Sox4 expression, which induces that of the histone methyltransferase, Ezh2, thereby reprogramming the epigenome to elicit EMT programs and metastasis of breast cancers. This viewpoint highlights Sox4 as a ‘new’ master of EMT programs and metastatic breast cancer.
Jenny G Parvani - One of the best experts on this subject based on the ideXlab platform.
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sox4 emt programs and the metastatic progression of breast cancers mastering the masters of emt
Breast Cancer Research, 2013Co-Authors: Jenny G Parvani, William P SchiemannAbstract:Epithelial-mesenchymal transition (EMT) programs require the expression of a variety of so-called master regulators of EMT, including members of the Snail, Zeb, and Twist Transcription Factor families. Teleologically, the requirement for such a diverse group of ‘master regulators’ seems evolutionarily cumbersome, and emerging evidence indicates that these Transcription Factors do in fact mediate unique and specialized functions, suggesting the existence of higher-order ‘masters’ that truly direct and coordinate EMT programs. Accordingly, Tiwari and colleagues recently delineated an elegant pathway wherein transforming growth Factor-beta stimulates Sox4 expression, which induces that of the histone methyltransferase, Ezh2, thereby reprogramming the epigenome to elicit EMT programs and metastasis of breast cancers. This viewpoint highlights Sox4 as a ‘new’ master of EMT programs and metastatic breast cancer.
Cazacu Eugeniu - One of the best experts on this subject based on the ideXlab platform.
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Epithelio-mesenchymal transition process in the pathogenesis of extragenital endometriosis
MedEspera, 2020Co-Authors: Cazacu Eugeniu, Vataman Vladimir, Pretula RuslanAbstract:Department of Morphopathology, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of Moldova, The 8th International Medical Congress for Students and Young Doctors, September 24-26, 2020Introduction. Epithelial – mesenchymal transition (EMT) endows cells with migratory and invasive proprieties, a prerequisite for the establishment of endometriotic lesions. The role EMT might play in the pathophysiology of endometriosis is still unknow. Therefore, we examined four markers for EMT in endometrium and endometriosis: E – cadherin + Vimentin, double reactions and simple reactions Twist and N – cadherin. Aim of the study. Immunohistochemical assessment of the invasiveness potential of extragenital endometriosis lesions by investigating some of the specific markers (β-catenin / vimentin panel) of the epithelio-mesenchymal transition process (EMT), a process by which epithelial cells lose their polarity and contact with the polarity and contact invasive. Materials and methods. During a period of five years (2012 – 2017) we analyzed 41 cases of extragenital endometriosis: appendix 5, colon 7, intestine 8, anterior abdominal wall after caesarean operation- 10, inguinal hernia – 6, umbilical hernia- 4, perineal region- 1. The material was processed according to the classic histological technique by inclusion in paraffin. The 3 μm sections obtained were stained with Hematoxylin – Eosin and Masson's trichrome stains. Another sections were dewaxed, rehydrated and processed for immunohistochemistry using as primary antibodies monoclonal antibodies Vimentin and mouse monoclonal antibody N – cadtherin, E – cadherin, Twist. Results. Immunohistochemically, we aimed to change the immunophenotype from epithelial to mesenchyme in extragenital endometriosis by analyzing the most important markers of the transition process. In endometriosis and endometrium E – cadherin, Vimentin, N – cadherin and Twist were expressed on protein level. Investigation of E – cadherin / Vimentin coexpression revealed a decrease in E – cadherin reactivity at the site of invasion of gastrointestinal endometriosis with an increase in reactivity to Vimentin together with the increase of the invasion pattern and the increase of the stage of the disease respectively. Twist Transcription Factor immunoexpression revealed a highly positive expression on the mesenchymal lineage, proving involvement of this Transcriptional Factor in the invasion process of endometriosis. N – cadherin was positive in the endometrial glands, showing their differentiation into a mesenchymal phenotype and their migratory potential. Conclusions. The results of our study confirm involvement of the epithelial – mesenchymal transition process in the pathogenesis of extragenital endometriosis lesions, on the one hand, and they certify their invasive potential in these localizations, on the other hand
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Study of the epithelio-mesenchymal transition process in the pathogenesis of gastrointestinal tract endometriosis
MedEspera, 2018Co-Authors: Cazacu EugeniuAbstract:Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova, Department of Morphopathology, Department of Pathology, University of Medicine and Pharmacy, Craiova, RomaniaIntroduction. Epithelial – mesenchymal transition (EMT) endows cells with migratory and invasive proprieties, a prerequisite for the establishment of endometriotic lesions. The role EMT might play in the pathophysiology of endometriosis is still unknow. Therefore, we examined four markers for EMT in endometrium and endometriosis: E – cadherin + Vimentin, double reactions and simple reactions Twist and N – cadherin. Aim of the study. The role EMT in the pathophysiology of endometriosis. Materials and methods. During a period of five years (2012 – 2017) we analyzed 7 cases of gastrointestinal tract endometriosis: appendix (1case), colon (5 cases), ileum (1case). The material was processed according to the classic histological technique by inclusion in paraffin. The 3 μm sections obtained were stained with Hematoxylin – Eosin and Masson’s trichrome stains. Another sections were dewaxed, rehydrated and processed for immunohistochemistry using as primary antibodies monoclonal antibodies Vimentin and mouse monoclonal antibody N – cadtherin, E – cadherin, Twist. Results. Immunohistochemically, we aimed to change the immunophenotype from epithelial to mesenchyme in gastrointestinal endometriosis by analyzing the most important markers of the transition process. In endometriosis and endometrium E – cadherin, Vimentin, N – cadherin and Twist were expressed on protein level. Investigation of E – cadherin / Vimentin coexpression revealed a decrease in E – cadherin reactivity at the site of invasion of gastrointestinal endometriosis with an increase in reactivity to Vimentin together with the increase of the invasion pattern and the increase of the stage of the disease respectively. Twist Transcription Factor immunoexpression revealed a highly positive expression on the mesenchymal lineage, proving involvement of this Transcriptional Factor in the invasion process of gastrointestinal endometriosis. N – cadherin was positive in the endometrial glands, showing their differentiation into a mesenchymal phenotype and their migratory potential. Conclusion: The results of our study confirm involvement of the epithelial – mesenchymal transition process in the pathogenesis
Pretula Ruslan - One of the best experts on this subject based on the ideXlab platform.
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Epithelio-mesenchymal transition process in the pathogenesis of extragenital endometriosis
MedEspera, 2020Co-Authors: Cazacu Eugeniu, Vataman Vladimir, Pretula RuslanAbstract:Department of Morphopathology, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, Republic of Moldova, The 8th International Medical Congress for Students and Young Doctors, September 24-26, 2020Introduction. Epithelial – mesenchymal transition (EMT) endows cells with migratory and invasive proprieties, a prerequisite for the establishment of endometriotic lesions. The role EMT might play in the pathophysiology of endometriosis is still unknow. Therefore, we examined four markers for EMT in endometrium and endometriosis: E – cadherin + Vimentin, double reactions and simple reactions Twist and N – cadherin. Aim of the study. Immunohistochemical assessment of the invasiveness potential of extragenital endometriosis lesions by investigating some of the specific markers (β-catenin / vimentin panel) of the epithelio-mesenchymal transition process (EMT), a process by which epithelial cells lose their polarity and contact with the polarity and contact invasive. Materials and methods. During a period of five years (2012 – 2017) we analyzed 41 cases of extragenital endometriosis: appendix 5, colon 7, intestine 8, anterior abdominal wall after caesarean operation- 10, inguinal hernia – 6, umbilical hernia- 4, perineal region- 1. The material was processed according to the classic histological technique by inclusion in paraffin. The 3 μm sections obtained were stained with Hematoxylin – Eosin and Masson's trichrome stains. Another sections were dewaxed, rehydrated and processed for immunohistochemistry using as primary antibodies monoclonal antibodies Vimentin and mouse monoclonal antibody N – cadtherin, E – cadherin, Twist. Results. Immunohistochemically, we aimed to change the immunophenotype from epithelial to mesenchyme in extragenital endometriosis by analyzing the most important markers of the transition process. In endometriosis and endometrium E – cadherin, Vimentin, N – cadherin and Twist were expressed on protein level. Investigation of E – cadherin / Vimentin coexpression revealed a decrease in E – cadherin reactivity at the site of invasion of gastrointestinal endometriosis with an increase in reactivity to Vimentin together with the increase of the invasion pattern and the increase of the stage of the disease respectively. Twist Transcription Factor immunoexpression revealed a highly positive expression on the mesenchymal lineage, proving involvement of this Transcriptional Factor in the invasion process of endometriosis. N – cadherin was positive in the endometrial glands, showing their differentiation into a mesenchymal phenotype and their migratory potential. Conclusions. The results of our study confirm involvement of the epithelial – mesenchymal transition process in the pathogenesis of extragenital endometriosis lesions, on the one hand, and they certify their invasive potential in these localizations, on the other hand
Yanchao Qin - One of the best experts on this subject based on the ideXlab platform.
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expressions of Twist Transcription Factor and e cadherin in anaplastic thyroid carcinoma and their relationship with the prognosis
Cancer Research and Clinic, 2019Co-Authors: Dongguang Qin, Yanchao QinAbstract:Objective To investigate the expressions of Twist Transcription Factor and E-cadherin in anaplastic thyroid carcinoma and their correlation with the prognosis. Methods The clinicopathological data of 20 patients with anaplastic thyroid carcinoma in Shanxi Provincial Cancer Hospital from May 2016 to April 2018 were retrospectively analyzed. The expressions of Twist protein and E-cadherin protein in anaplastic thyroid carcinoma tissues and corresponding paracancerous tissues were detected by using immunohistochemistry. The relationship between the expressions of the two proteins and the clinicopathological features of the patients was analyzed, and the correlation among the expressions of the two proteins as well as the clinicopathological Factors and the prognosis of the patients was analyzed. Results The positive expression rate of Twist protein in anaplastic thyroid carcinoma tissues was 65% (13/20), while it was 20% (4/20) in paracancerous tissues, and the difference was statistically significant (P=0.004). The positive expression rate of E-cadherin in anaplastic thyroid carcinoma tissues was 60% (12/20), while it was 100% (20/20) in paracancerous tissues, and the difference was statistically significant (P=0.001). There was a negative correlation between the expression of Twist protein and E-cadherin protein in anaplastic thyroid carcinoma (r=-0.685, P=0.001). The expression of Twist protein in anaplastic thyroid carcinoma was correlated with lymph node metastasis and TNM stage (both P 0.05). The expression of E-cadherin protein in anaplastic thyroid carcinoma had no correlation with clinicopathological Factors (all P >0.05). The results of Kaplan-Meier survival analysis showed that lymph node metastasis (P < 0.01), TNM stage (P=0.002) and the expression of Twist protein (P=0.017) were prognostic Factors in patients with anaplastic thyroid carcinoma. Conclusions The expression of Twist Transcription Factor is upregulated in anaplastic thyroid carcinoma, and the expression of E-cadherin is downregulated. The expression of Twist protein is correlated with the prognosis of the patients. The epithelial-mesenchymal transition may be involved in the progress of anaplastic thyroid carcinoma. Key words: Anaplastic thyroid carcinoma; Epithelial-mesenchymal transition; Immunohistochemistry
