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Mark Varrelmann - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel mycovirus isolated from Rhizoctonia solani (AG 2-2 IV) provides further information about genome plasticity within the order Tymovirales
Archives of Virology, 2017Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The complete genome of a novel mycovirus, named Rhizoctonia solani flexivirus 1 (RsFV-1), which infects an avirulent strain of Rhizoctonia solani AG 2-2 IV, was sequenced and analyzed. Its RNA genome consists of 10,621 nucleotides, excluding the poly-A tail, and encodes a single protein of 3477 amino acids. The identification of conserved motifs of methyltransferase, helicase and RNA-dependent RNA polymerase revealed its relatedness to members of the alphavirus-like superfamily of positive-strand RNA viruses. Phylogenetic analysis of these fused domains suggested that this virus should be assigned to the order Tymovirales . The recently described Fusarium graminearum deltaflexivirus 1 was found to be its closest relative. However, the whole genome, as well as the encoded protein of RsFV-1, is larger than that of other known members of the order Tymovirales , and unlike all other viruses belonging to this order, its methyltransferase domain is not located at the N-terminus of the replicase. Although genome diversity, as a result of recombination and gene loss, is a well-documented trait in members of the order Tymovirales , no related virus with a comparable genome alteration has been reported before. For these reasons, RsFV-1 broadens our perception about genome plasticity and diversity within the order Tymovirales .
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Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV
PLOS ONE, 2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNA-polymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.
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Identification of the conserved motifs A to G in the RdRp domain of the type species of all genera within the order Tymovirales.
2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The RdRp domains of Rhizoctonia solani flexivirus 1 (RsFV-1) (KX349064), Rhizoctonia solani flexivirus 2 (RSFV-2) (KX349069) and the type species of all genera within the Tymovirales [8] were aligned using MEGA 6.06 and the sequence alignment algorithm MUSCLE [44]. Conserved motifs A to G were marked according to Bruenn 2003, Černý et al. 2014, Koonin et al. 1993, Boehr et al. 2014 and Xu et al. 2003 [30, 34, 42–44]. ACLSV, Apple chlorotic leaf spot virus (ABY71563.1); AcLV, Aconitum latent virus (NP_116487.1); ASGV, Apple stem grooving virus (BAA98054.1); ASPV, Apple stem pitting virus (AEP02955.1); BotV-F, Botrytis virus F (NP_068549.1); BotV-X, Botrytis virus X (NP_932306.1); CLBV, Citrus leaf blotch virus (NP_624333.1); GFkV, Grapevine fleck virus (NP_542612.1); GVA, Grapevine virus A (AAO17778.1); ICRSV, Indian citrus ringspot virus (NP_203553.1); LoLV, Lolium latent virus (YP_001718499.1); MRFV, Maize rayado fino virus (NP_115454.1); PVT, Potato virus T (BAM16482.1); PVX, Potato virus X (AAF89747.1); SsDRV, Sclerotinia sclerotiorum debilitation-associated RNA virus (YP_325662.1); ShVX, Shallot virus X (NP_620648.1). Shading indicates level of conservation and the consensus sequence is displayed.
Anika Bartholomäus - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel mycovirus isolated from Rhizoctonia solani (AG 2-2 IV) provides further information about genome plasticity within the order Tymovirales
Archives of Virology, 2017Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The complete genome of a novel mycovirus, named Rhizoctonia solani flexivirus 1 (RsFV-1), which infects an avirulent strain of Rhizoctonia solani AG 2-2 IV, was sequenced and analyzed. Its RNA genome consists of 10,621 nucleotides, excluding the poly-A tail, and encodes a single protein of 3477 amino acids. The identification of conserved motifs of methyltransferase, helicase and RNA-dependent RNA polymerase revealed its relatedness to members of the alphavirus-like superfamily of positive-strand RNA viruses. Phylogenetic analysis of these fused domains suggested that this virus should be assigned to the order Tymovirales . The recently described Fusarium graminearum deltaflexivirus 1 was found to be its closest relative. However, the whole genome, as well as the encoded protein of RsFV-1, is larger than that of other known members of the order Tymovirales , and unlike all other viruses belonging to this order, its methyltransferase domain is not located at the N-terminus of the replicase. Although genome diversity, as a result of recombination and gene loss, is a well-documented trait in members of the order Tymovirales , no related virus with a comparable genome alteration has been reported before. For these reasons, RsFV-1 broadens our perception about genome plasticity and diversity within the order Tymovirales .
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Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV
PLOS ONE, 2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNA-polymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.
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Identification of the conserved motifs A to G in the RdRp domain of the type species of all genera within the order Tymovirales.
2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The RdRp domains of Rhizoctonia solani flexivirus 1 (RsFV-1) (KX349064), Rhizoctonia solani flexivirus 2 (RSFV-2) (KX349069) and the type species of all genera within the Tymovirales [8] were aligned using MEGA 6.06 and the sequence alignment algorithm MUSCLE [44]. Conserved motifs A to G were marked according to Bruenn 2003, Černý et al. 2014, Koonin et al. 1993, Boehr et al. 2014 and Xu et al. 2003 [30, 34, 42–44]. ACLSV, Apple chlorotic leaf spot virus (ABY71563.1); AcLV, Aconitum latent virus (NP_116487.1); ASGV, Apple stem grooving virus (BAA98054.1); ASPV, Apple stem pitting virus (AEP02955.1); BotV-F, Botrytis virus F (NP_068549.1); BotV-X, Botrytis virus X (NP_932306.1); CLBV, Citrus leaf blotch virus (NP_624333.1); GFkV, Grapevine fleck virus (NP_542612.1); GVA, Grapevine virus A (AAO17778.1); ICRSV, Indian citrus ringspot virus (NP_203553.1); LoLV, Lolium latent virus (YP_001718499.1); MRFV, Maize rayado fino virus (NP_115454.1); PVT, Potato virus T (BAM16482.1); PVX, Potato virus X (AAF89747.1); SsDRV, Sclerotinia sclerotiorum debilitation-associated RNA virus (YP_325662.1); ShVX, Shallot virus X (NP_620648.1). Shading indicates level of conservation and the consensus sequence is displayed.
Andreas Schluter - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel mycovirus isolated from Rhizoctonia solani (AG 2-2 IV) provides further information about genome plasticity within the order Tymovirales
Archives of Virology, 2017Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The complete genome of a novel mycovirus, named Rhizoctonia solani flexivirus 1 (RsFV-1), which infects an avirulent strain of Rhizoctonia solani AG 2-2 IV, was sequenced and analyzed. Its RNA genome consists of 10,621 nucleotides, excluding the poly-A tail, and encodes a single protein of 3477 amino acids. The identification of conserved motifs of methyltransferase, helicase and RNA-dependent RNA polymerase revealed its relatedness to members of the alphavirus-like superfamily of positive-strand RNA viruses. Phylogenetic analysis of these fused domains suggested that this virus should be assigned to the order Tymovirales . The recently described Fusarium graminearum deltaflexivirus 1 was found to be its closest relative. However, the whole genome, as well as the encoded protein of RsFV-1, is larger than that of other known members of the order Tymovirales , and unlike all other viruses belonging to this order, its methyltransferase domain is not located at the N-terminus of the replicase. Although genome diversity, as a result of recombination and gene loss, is a well-documented trait in members of the order Tymovirales , no related virus with a comparable genome alteration has been reported before. For these reasons, RsFV-1 broadens our perception about genome plasticity and diversity within the order Tymovirales .
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Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV
PLOS ONE, 2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNA-polymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.
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Identification of the conserved motifs A to G in the RdRp domain of the type species of all genera within the order Tymovirales.
2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The RdRp domains of Rhizoctonia solani flexivirus 1 (RsFV-1) (KX349064), Rhizoctonia solani flexivirus 2 (RSFV-2) (KX349069) and the type species of all genera within the Tymovirales [8] were aligned using MEGA 6.06 and the sequence alignment algorithm MUSCLE [44]. Conserved motifs A to G were marked according to Bruenn 2003, Černý et al. 2014, Koonin et al. 1993, Boehr et al. 2014 and Xu et al. 2003 [30, 34, 42–44]. ACLSV, Apple chlorotic leaf spot virus (ABY71563.1); AcLV, Aconitum latent virus (NP_116487.1); ASGV, Apple stem grooving virus (BAA98054.1); ASPV, Apple stem pitting virus (AEP02955.1); BotV-F, Botrytis virus F (NP_068549.1); BotV-X, Botrytis virus X (NP_932306.1); CLBV, Citrus leaf blotch virus (NP_624333.1); GFkV, Grapevine fleck virus (NP_542612.1); GVA, Grapevine virus A (AAO17778.1); ICRSV, Indian citrus ringspot virus (NP_203553.1); LoLV, Lolium latent virus (YP_001718499.1); MRFV, Maize rayado fino virus (NP_115454.1); PVT, Potato virus T (BAM16482.1); PVX, Potato virus X (AAF89747.1); SsDRV, Sclerotinia sclerotiorum debilitation-associated RNA virus (YP_325662.1); ShVX, Shallot virus X (NP_620648.1). Shading indicates level of conservation and the consensus sequence is displayed.
Anika Winkler - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel mycovirus isolated from Rhizoctonia solani (AG 2-2 IV) provides further information about genome plasticity within the order Tymovirales
Archives of Virology, 2017Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The complete genome of a novel mycovirus, named Rhizoctonia solani flexivirus 1 (RsFV-1), which infects an avirulent strain of Rhizoctonia solani AG 2-2 IV, was sequenced and analyzed. Its RNA genome consists of 10,621 nucleotides, excluding the poly-A tail, and encodes a single protein of 3477 amino acids. The identification of conserved motifs of methyltransferase, helicase and RNA-dependent RNA polymerase revealed its relatedness to members of the alphavirus-like superfamily of positive-strand RNA viruses. Phylogenetic analysis of these fused domains suggested that this virus should be assigned to the order Tymovirales . The recently described Fusarium graminearum deltaflexivirus 1 was found to be its closest relative. However, the whole genome, as well as the encoded protein of RsFV-1, is larger than that of other known members of the order Tymovirales , and unlike all other viruses belonging to this order, its methyltransferase domain is not located at the N-terminus of the replicase. Although genome diversity, as a result of recombination and gene loss, is a well-documented trait in members of the order Tymovirales , no related virus with a comparable genome alteration has been reported before. For these reasons, RsFV-1 broadens our perception about genome plasticity and diversity within the order Tymovirales .
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Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV
PLOS ONE, 2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNA-polymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.
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Identification of the conserved motifs A to G in the RdRp domain of the type species of all genera within the order Tymovirales.
2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The RdRp domains of Rhizoctonia solani flexivirus 1 (RsFV-1) (KX349064), Rhizoctonia solani flexivirus 2 (RSFV-2) (KX349069) and the type species of all genera within the Tymovirales [8] were aligned using MEGA 6.06 and the sequence alignment algorithm MUSCLE [44]. Conserved motifs A to G were marked according to Bruenn 2003, Černý et al. 2014, Koonin et al. 1993, Boehr et al. 2014 and Xu et al. 2003 [30, 34, 42–44]. ACLSV, Apple chlorotic leaf spot virus (ABY71563.1); AcLV, Aconitum latent virus (NP_116487.1); ASGV, Apple stem grooving virus (BAA98054.1); ASPV, Apple stem pitting virus (AEP02955.1); BotV-F, Botrytis virus F (NP_068549.1); BotV-X, Botrytis virus X (NP_932306.1); CLBV, Citrus leaf blotch virus (NP_624333.1); GFkV, Grapevine fleck virus (NP_542612.1); GVA, Grapevine virus A (AAO17778.1); ICRSV, Indian citrus ringspot virus (NP_203553.1); LoLV, Lolium latent virus (YP_001718499.1); MRFV, Maize rayado fino virus (NP_115454.1); PVT, Potato virus T (BAM16482.1); PVX, Potato virus X (AAF89747.1); SsDRV, Sclerotinia sclerotiorum debilitation-associated RNA virus (YP_325662.1); ShVX, Shallot virus X (NP_620648.1). Shading indicates level of conservation and the consensus sequence is displayed.
Alfred Puhler - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel mycovirus isolated from Rhizoctonia solani (AG 2-2 IV) provides further information about genome plasticity within the order Tymovirales
Archives of Virology, 2017Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The complete genome of a novel mycovirus, named Rhizoctonia solani flexivirus 1 (RsFV-1), which infects an avirulent strain of Rhizoctonia solani AG 2-2 IV, was sequenced and analyzed. Its RNA genome consists of 10,621 nucleotides, excluding the poly-A tail, and encodes a single protein of 3477 amino acids. The identification of conserved motifs of methyltransferase, helicase and RNA-dependent RNA polymerase revealed its relatedness to members of the alphavirus-like superfamily of positive-strand RNA viruses. Phylogenetic analysis of these fused domains suggested that this virus should be assigned to the order Tymovirales . The recently described Fusarium graminearum deltaflexivirus 1 was found to be its closest relative. However, the whole genome, as well as the encoded protein of RsFV-1, is larger than that of other known members of the order Tymovirales , and unlike all other viruses belonging to this order, its methyltransferase domain is not located at the N-terminus of the replicase. Although genome diversity, as a result of recombination and gene loss, is a well-documented trait in members of the order Tymovirales , no related virus with a comparable genome alteration has been reported before. For these reasons, RsFV-1 broadens our perception about genome plasticity and diversity within the order Tymovirales .
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Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV
PLOS ONE, 2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNA-polymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.
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Identification of the conserved motifs A to G in the RdRp domain of the type species of all genera within the order Tymovirales.
2016Co-Authors: Anika Bartholomäus, Anika Winkler, Andreas Schluter, Daniel Wibberg, Alfred Puhler, Mark VarrelmannAbstract:The RdRp domains of Rhizoctonia solani flexivirus 1 (RsFV-1) (KX349064), Rhizoctonia solani flexivirus 2 (RSFV-2) (KX349069) and the type species of all genera within the Tymovirales [8] were aligned using MEGA 6.06 and the sequence alignment algorithm MUSCLE [44]. Conserved motifs A to G were marked according to Bruenn 2003, Černý et al. 2014, Koonin et al. 1993, Boehr et al. 2014 and Xu et al. 2003 [30, 34, 42–44]. ACLSV, Apple chlorotic leaf spot virus (ABY71563.1); AcLV, Aconitum latent virus (NP_116487.1); ASGV, Apple stem grooving virus (BAA98054.1); ASPV, Apple stem pitting virus (AEP02955.1); BotV-F, Botrytis virus F (NP_068549.1); BotV-X, Botrytis virus X (NP_932306.1); CLBV, Citrus leaf blotch virus (NP_624333.1); GFkV, Grapevine fleck virus (NP_542612.1); GVA, Grapevine virus A (AAO17778.1); ICRSV, Indian citrus ringspot virus (NP_203553.1); LoLV, Lolium latent virus (YP_001718499.1); MRFV, Maize rayado fino virus (NP_115454.1); PVT, Potato virus T (BAM16482.1); PVX, Potato virus X (AAF89747.1); SsDRV, Sclerotinia sclerotiorum debilitation-associated RNA virus (YP_325662.1); ShVX, Shallot virus X (NP_620648.1). Shading indicates level of conservation and the consensus sequence is displayed.
