The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform
Andrada-serpa Mj - One of the best experts on this subject based on the ideXlab platform.
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EleCtron miCrosCopiC observation of a Brazilian HTLV-I isolated.
Journal of submicroscopic cytology and pathology, 1998Co-Authors: Taffarel M, Andrada-serpa MjAbstract:: Several studies Carried out in Brazil have shown a high inCidenCe of HTLV-I infeCtion among the general population and in different groups, suCh as blood donors, hemophiliaCs, hematologiCal and neurologiCal patients. Cases of adult T-Cell leukemia/lymphoma as well as tropiCal spastiC paraparesis/HTLV-I assoCiated myelopathy have already been desCribed. Therefore, it is important to CharaCterize Brazilian HTLV strains using different teChniCal approaChes. The purpose of this paper is to CharaCterize and reCognize HTLV partiCles employing routine and immunoeleCtron miCrosCopy in lymphoCyte CoCultures. UltrastruCtural analysis showed typiCal large and small virus partiCles in Close relation with the lymphoCyte membrane. ImmunoeleCtron miCrosCopy, Carried out with HTLV positive sera, allowed the identifiCation of the virus as a Type C onCovirus, group HTLV-BLV. The first interaCtion events between virus and lymphoCyte membrane have also been analysed and struCtures related to the endoCytiC route for HTLV entranCe were pointed out.
Taffarel M - One of the best experts on this subject based on the ideXlab platform.
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EleCtron miCrosCopiC observation of a Brazilian HTLV-I isolated.
Journal of submicroscopic cytology and pathology, 1998Co-Authors: Taffarel M, Andrada-serpa MjAbstract:: Several studies Carried out in Brazil have shown a high inCidenCe of HTLV-I infeCtion among the general population and in different groups, suCh as blood donors, hemophiliaCs, hematologiCal and neurologiCal patients. Cases of adult T-Cell leukemia/lymphoma as well as tropiCal spastiC paraparesis/HTLV-I assoCiated myelopathy have already been desCribed. Therefore, it is important to CharaCterize Brazilian HTLV strains using different teChniCal approaChes. The purpose of this paper is to CharaCterize and reCognize HTLV partiCles employing routine and immunoeleCtron miCrosCopy in lymphoCyte CoCultures. UltrastruCtural analysis showed typiCal large and small virus partiCles in Close relation with the lymphoCyte membrane. ImmunoeleCtron miCrosCopy, Carried out with HTLV positive sera, allowed the identifiCation of the virus as a Type C onCovirus, group HTLV-BLV. The first interaCtion events between virus and lymphoCyte membrane have also been analysed and struCtures related to the endoCytiC route for HTLV entranCe were pointed out.
Laurence N. Payne - One of the best experts on this subject based on the ideXlab platform.
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Biology of Avian Retroviruses
The Retroviridae, 1992Co-Authors: Laurence N. PayneAbstract:Avian retroviruses have, in the past, been plaCed taxonomiCally in a subgenus termed vernaCularly “avian Type C onCoviruses” of the genus Type C onCovirus group, within the subfamily OnCovirinae, family Retroviridae (Matthews, 1982). The reCent new ClassifiCation of retroviruses plaCe them into a separate genus (see Coffin, Chapter 2). Four distinCt “speCies” of avian retrovirus have been desCribed (Matthews, 1982; Porterfield, 1989): 1. Leukosis-sarComa group viruses (also termed avian sarComa and leukemia viruses). These are exogenous and endogenous viruses reCognized mainly in the domestiC fowl and whiCh Cause a variety of leukotiC disorders, sarComas, and other tumors. 2. RetiCuloendotheliosis viruses. These are exogenous viruses in several speCies of domestiCated poultry and appear to be related to mammalian retroviruses. They Cause lymphomas and aCute retiCulum Cell and other tumors. 3. Lymphoproliferative disease virus of turkeys. This is an exogenous virus of turkeys whiCh Causes a lymphoproliferative disease. 4. Pheasant Type C onCoviruses. These are endogenous viruses of golden and Lady Amhurst pheasants and are apparently non-pathogeniC. They are unrelated to the endogenous leukosissarComa group viruses whiCh also oCCur in Certain speCies of pheasants.
Dominique Rigal - One of the best experts on this subject based on the ideXlab platform.
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Canine large granular lymphoCyte leukemia and its derived Cell line produCe infeCtious retroviral partiCles.
Veterinary Pathology, 2000Co-Authors: I. Ghernati, Antoine Corbin, Luc Chabanne, C. Auger, J.p. Magnol, C. Fournel, J. C. Monier, J. L. Darlix, Dominique RigalAbstract:We desCribe a Case of large granular lymphoCyte (LGL) leukemia in a dog that we followed over a period of 2 years. Analysis of a hematologiCal profile revealed lymphoCytosis (19,500 lymphoCytes per miCroliter; referenCe values, 1,000-4,800 lymphoCytes per miCroliter), with a majority of LGL on the blood smear. LGL is defined as a lymphoid subset Comprising 10% of peripheral blood mononuClear Cells and Cor- responding to either CD3 CD8 NK Cells or CD3 CD8 T Cells. The Cells are CharaCterized by abundant basophiliC Cytoplasm Containing distinCt granules of variable size and number. The CharaCteristiC phenoType of our leukemiC LGL is of a CytotoxiC T Cell, CD3 and CD8. A new Cell line, DLC 02, was established from the peripheral lymphoCytes of the leukemiC dog. PartiCles with Type C retroviral morphology were found in ultrathin seCtions of DLC 02 Cell pellets. These partiCles were found to have a suCrose gradient density of 1.17 g/liter and a reverse transCriptase aCtivity with an Mn 2 preferenCe, suggesting that they Correspond to a mam- malian Type C onCovirus.
I. Ghernati - One of the best experts on this subject based on the ideXlab platform.
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Canine large granular lymphoCyte leukemia and its derived Cell line produCe infeCtious retroviral partiCles.
Veterinary Pathology, 2000Co-Authors: I. Ghernati, Antoine Corbin, Luc Chabanne, C. Auger, J.p. Magnol, C. Fournel, J. C. Monier, J. L. Darlix, Dominique RigalAbstract:We desCribe a Case of large granular lymphoCyte (LGL) leukemia in a dog that we followed over a period of 2 years. Analysis of a hematologiCal profile revealed lymphoCytosis (19,500 lymphoCytes per miCroliter; referenCe values, 1,000-4,800 lymphoCytes per miCroliter), with a majority of LGL on the blood smear. LGL is defined as a lymphoid subset Comprising 10% of peripheral blood mononuClear Cells and Cor- responding to either CD3 CD8 NK Cells or CD3 CD8 T Cells. The Cells are CharaCterized by abundant basophiliC Cytoplasm Containing distinCt granules of variable size and number. The CharaCteristiC phenoType of our leukemiC LGL is of a CytotoxiC T Cell, CD3 and CD8. A new Cell line, DLC 02, was established from the peripheral lymphoCytes of the leukemiC dog. PartiCles with Type C retroviral morphology were found in ultrathin seCtions of DLC 02 Cell pellets. These partiCles were found to have a suCrose gradient density of 1.17 g/liter and a reverse transCriptase aCtivity with an Mn 2 preferenCe, suggesting that they Correspond to a mam- malian Type C onCovirus.
