Tyrodes Solution

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Patrick Azubuike Onyeyili - One of the best experts on this subject based on the ideXlab platform.

  • carbachol following Escherichia coli infection in chickens
    2016
    Co-Authors: Saka Sanni, Patrick Azubuike Onyeyili, Usman Bala Shamaki
    Abstract:

    muscle to histamine and carbachol following Escherichia coli infection in chickens. Vet. arhiv 75, 423-429, 2005. The contractile responses of the duodenal segments from healthy and Escherichia coli infected broiler chickens on exposure to exogenous histamine and carbachol were studied. Molar concentrations of the agonists were introduced into suspensions of the segments in Tyrodes Solution at 37 oC and the responses recorded using kymograph. Both healthy and infected segments responded dose-dependently to the agonists. The healthy segments showed greater sensitivity to the agonists and produced significantly (P<0.05) higher mean maximum responses in all cases. The threshold responses to molar concentrations of the drugs were lower for segments from infected chickens. It is suggested that E. coli infection may have altered the contractile responses of chicken duodenum to the agonists. Key words: chickens, E. coli, histamine, carbacho

Bala Shamaki Usman - One of the best experts on this subject based on the ideXlab platform.

Naciye Yaktubay Dondas - One of the best experts on this subject based on the ideXlab platform.

  • The relaxant responses induced by ethanol in the smooth muscle: role of extracellular and intracellular Ca2+ [Duz kasta etanol ile induklenen gevseme cevaplari: ekstraseluler ve intraseluler Ca2+un rolu]
    Society of TURAZ AKADEMI, 2016
    Co-Authors: Derya Kaya, Naciye Yaktubay Dondas
    Abstract:

    Since worldwide alcohol consumption, the understanding the mechanism of action of ethanol is important in the gastrointestinal tract. In this study we aimed to clarify the role of extracellular and intracellular calcium (Ca2+) on relaxations induced by ethanol in isolated mouse gastric fundus. Mice (Swiss albino) of either sex were used in this study. After killing the mice by cervical dislocation the gastric fundal strips were prepared by longitudinal incision and mounted under 0.5 g tension in an organ bath filled with Tyrodes Solution. The bath medium was maintained at 37 oC and gassed with %95O2 and 5%CO2. Experimental data were recorded by an isometric transducer. Ethanol (164 mM) caused reproducible relaxations in isolated mouse gastric fundal strips. These relaxations were significantly inhibited by verapamil (10-5-5x10-4 M), a blocker of L-type Ca2+ channels, and ruthenium red (10-5-10-4 M), a blocker of ryanodine receptors (intracellular Ca2+ channels) in a concentration dependent manner. On the other hand cyclopiasonic acid (CPA; 10-6-10-5 M), a blocker of Ca2+-ATPase, failed to affect the relaxations induced by ethanol. The results of experimental data suggest that extracellular Ca2+ influx and intracellular Ca2+ release may play a role on relaxations induced by ethanol in the isolated mouse gastric fundal smooth muscle. [Med-Science 2016; 5(4.000): 967-71

Saka Sanni - One of the best experts on this subject based on the ideXlab platform.

  • carbachol following Escherichia coli infection in chickens
    2016
    Co-Authors: Saka Sanni, Patrick Azubuike Onyeyili, Usman Bala Shamaki
    Abstract:

    muscle to histamine and carbachol following Escherichia coli infection in chickens. Vet. arhiv 75, 423-429, 2005. The contractile responses of the duodenal segments from healthy and Escherichia coli infected broiler chickens on exposure to exogenous histamine and carbachol were studied. Molar concentrations of the agonists were introduced into suspensions of the segments in Tyrodes Solution at 37 oC and the responses recorded using kymograph. Both healthy and infected segments responded dose-dependently to the agonists. The healthy segments showed greater sensitivity to the agonists and produced significantly (P<0.05) higher mean maximum responses in all cases. The threshold responses to molar concentrations of the drugs were lower for segments from infected chickens. It is suggested that E. coli infection may have altered the contractile responses of chicken duodenum to the agonists. Key words: chickens, E. coli, histamine, carbacho

Sanni Saka - One of the best experts on this subject based on the ideXlab platform.