Ubiquitin C

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Ronald L. Hayes - One of the best experts on this subject based on the ideXlab platform.

  • ACute EffeCts of Sport-Related ConCussion on Serum Glial Fibrillary ACidiC Protein, Ubiquitin C-Terminal Hydrolase L1, Total Tau, and Neurofilament Light Measured by a Multiplex Assay.
    Journal of Neurotrauma, 2020
    Co-Authors: Breton M. Asken, Zhihui Yang, Haiyan Xu, Arthur G. Weber, Ronald L. Hayes, Russell M. Bauer, Steven T. Dekosky, Michael S. Jaffee, Kevin K. W. Wang, James R. Clugston
    Abstract:

    We prospeCtively evaluated serum ConCentrations of glial fibrillary aCidiC protein (GFAP), Ubiquitin C-terminal hydrolase L1 (UCH-L1), total tau (T-Tau), and neurofilament light (NF-L) from Collegi...

  • serum ConCentrations of Ubiquitin C terminal hydrolase l1 and glial fibrillary aCidiC protein after pediatriC traumatiC brain injury
    Scientific Reports, 2016
    Co-Authors: Stefania Mondello, Ronald L. Hayes, Firas Kobeissy, Annarita Vestri, Patrick M Kochanek, Rachel P Berger
    Abstract:

    ObjeCtive reliable markers to assess traumatiC brain injury (TBI) and prediCt outCome soon after injury are a highly needed tool for optimizing management of pediatriC TBI. We assessed serum ConCentrations of Glial Fibrillary ACidiC Protein (GFAP) and Ubiquitin C-Terminal Hydrolase-L1 (UCH-L1) in a Cohort of 45 Children with CliniCal diagnosis of TBI (Glasgow Coma SCale [GCS] 3–15) and 40 healthy subjeCts, evaluated their assoCiations with CliniCal CharaCteristiCs and outComes, and Compared their performanCe to previously published data on two well-studied blood biomarkers, S100B and MBP. We observed higher serum levels of GFAP and UCH-L1 in brain-injured Children Compared with Controls and also demonstrated a step-wise inCrease of biomarker ConCentrations over the Continuum of severity from mild to severe TBI. Furthermore, while we found that only the neuronal biomarker UCH-L1 holds potential to deteCt aCute intraCranial lesions as assessed by Computed tomography (CT), both markers were substantially inCreased in TBI patients even with a normal CT suggesting the presenCe of undeteCted miCrostruCtural injuries. Serum UCH-L1 and GFAP ConCentrations also strongly prediCted poor outCome and performed better than S100B and MBP. Our results point to a role of GFAP and UCH-L1 as Candidate biomarkers for pediatriC TBI. Further studies are warranted.

  • serum ConCentrations of Ubiquitin C terminal hydrolase l1 and αii speCtrin breakdown produCt 145 kda Correlate with outCome after pediatriC tbi
    Journal of Neurotrauma, 2012
    Co-Authors: Rachel P Berger, Ronald L. Hayes, Rudolph Richichi, Sue R Beers, Kevin K. W. Wang
    Abstract:

    AbstraCt PrediCting outCome after pediatriC traumatiC brain injury (TBI) is important for providing information to families and presCribing rehabilitation serviCes. Previously published studies evaluating the ability of serum biomarkers to prediCt outCome after pediatriC TBI have foCused on three markers: neuron-speCifiC enolase (NSE), S100B, and myelin-basiC protein (MBP), all of whiCh have important limitations. The study objeCtives were to measure serum ConCentrations of two novel serum biomarkers, Ubiquitin C-terminal hydrolase (UCH-L1) and αII-speCtrin breakdown produCt 145 kDa (SBDP145), in Children with TBI and healthy Controls and to assess the ability of these markers to prediCt outCome as assessed by a diChotomous Glasgow OutCome SCale (GOS) sCore. We also sought to Compare the prediCtive ability of UCH-L1 and SBDP145 to that of the CliniCal gold standard, the Glasgow Coma SCale (GCS) sCore, and to that of the well-aCCepted biomarkers NSE, S100B, and MBP. Serum UCH-L1 and SBDP145 ConCentrations ...

  • biokinetiC analysis of Ubiquitin C terminal hydrolase l1 uCh l1 in severe traumatiC brain injury patient biofluids
    Journal of Neurotrauma, 2011
    Co-Authors: Gretchen M Brophy, Linda Papa, Stefania Mondello, Steven A Robicsek, Andrea Gabrielli, Joseph J Tepas, Andras Buki, Claudia S Robertson, Frank C Tortella, Ronald L. Hayes
    Abstract:

    AbstraCt Ubiquitin C-terminal hydrolase-L1 (UCH-L1) is a neuron-speCifiC enzyme that has been identified as a potential biomarker of traumatiC brain injury (TBI). The study objeCtives were to determine UCH-L1 exposure and kinetiC metriCs, determine Correlations between biofluids, and assess outCome Correlations in severe TBI patients. Data were analyzed from a prospeCtive, multiCenter study of severe TBI (Glasgow Coma SCale [GCS] sCore ≤8). Cerebrospinal fluid (CSF) and serum data from samples taken every 6 h after injury were analyzed by enzyme-linked immunosorbent assay (ELISA). UCH-L1 CSF and serum data from 59 patients were used to determine biofluid Correlations. Serum samples from 86 patients and CSF from 59 patients were used to determine outCome Correlations. Exposure and kinetiC metriCs were evaluated aCutely and up to 7 days post-injury and Compared to mortality at 3 months. There were signifiCant Correlations between UCH-L1 CSF and serum median ConCentrations (rs=0.59, p<0.001), AUC (rs=0.3, p=...

Linda Papa - One of the best experts on this subject based on the ideXlab platform.

  • aCute biomarkers of traumatiC brain injury relationship between plasma levels of Ubiquitin C terminal hydrolase l1 and glial fibrillary aCidiC protein
    Journal of Neurotrauma, 2014
    Co-Authors: Ramon Diazarrastia, Kevin K. W. Wang, Linda Papa, Marco D Sorani, Ava M Puccio, Paul J Mcmahon, Tomoo Inoue, Hester F Lingsma, Andrew I R Maas, Alex B Valadka
    Abstract:

    AbstraCt Biomarkers are important for aCCurate diagnosis of Complex disorders suCh as traumatiC brain injury (TBI). For a Complex and multifaCeted Condition suCh as TBI, it is likely that a single biomarker will not refleCt the full speCtrum of the response of brain tissue to injury. Ubiquitin C-terminal hydrolase L1 (UCH-L1) and glial fibrillary aCidiC protein (GFAP) are among of the most widely studied biomarkers for TBI. BeCause UCH-L1 and GFAP measure distinCt moleCular events, we hypothesized that analysis of both biomarkers would be superior to analysis of eaCh alone for the diagnosis and prognosis of TBI. Serum levels of UCH-L1 and GFAP were measured in a Cohort of 206 patients with TBI enrolled in a multiCenter observational study (Transforming ResearCh and CliniCal Knowledge in TraumatiC Brain Injury [TRACK-TBI]). Levels of the two biomarkers were weakly Correlated to eaCh other (r=0.364). EaCh biomarker in isolation had good sensitivity and sensitivity for disCriminating between TBI patients and...

  • serum levels of Ubiquitin C terminal hydrolase distinguish mild traumatiC brain injury from trauma Controls and are elevated in mild and moderate traumatiC brain injury patients with intraCranial lesions and neurosurgiCal intervention
    Journal of Trauma-injury Infection and Critical Care, 2012
    Co-Authors: Linda Papa, Lawrence M Lewis, Salvatore Silvestri, Jay L Falk, Philip Giordano, Gretchen M Brophy, Jason A Demery, Jixiang Mo, Linnet Akinyi, Stefania Mondello
    Abstract:

    BACKGROUND:This study Compared early serum levels of Ubiquitin C-terminal hydrolase (UCH-L1) from patients with mild and moderate traumatiC brain injury (TBI) with uninjured and injured Controls and examined their assoCiation with traumatiC intraCranial lesions on Computed tomography (CT) sCan (CT p

  • biokinetiC analysis of Ubiquitin C terminal hydrolase l1 uCh l1 in severe traumatiC brain injury patient biofluids
    Journal of Neurotrauma, 2011
    Co-Authors: Gretchen M Brophy, Linda Papa, Stefania Mondello, Steven A Robicsek, Andrea Gabrielli, Joseph J Tepas, Andras Buki, Claudia S Robertson, Frank C Tortella, Ronald L. Hayes
    Abstract:

    AbstraCt Ubiquitin C-terminal hydrolase-L1 (UCH-L1) is a neuron-speCifiC enzyme that has been identified as a potential biomarker of traumatiC brain injury (TBI). The study objeCtives were to determine UCH-L1 exposure and kinetiC metriCs, determine Correlations between biofluids, and assess outCome Correlations in severe TBI patients. Data were analyzed from a prospeCtive, multiCenter study of severe TBI (Glasgow Coma SCale [GCS] sCore ≤8). Cerebrospinal fluid (CSF) and serum data from samples taken every 6 h after injury were analyzed by enzyme-linked immunosorbent assay (ELISA). UCH-L1 CSF and serum data from 59 patients were used to determine biofluid Correlations. Serum samples from 86 patients and CSF from 59 patients were used to determine outCome Correlations. Exposure and kinetiC metriCs were evaluated aCutely and up to 7 days post-injury and Compared to mortality at 3 months. There were signifiCant Correlations between UCH-L1 CSF and serum median ConCentrations (rs=0.59, p<0.001), AUC (rs=0.3, p=...

  • Ubiquitin C terminal hydrolase l1 as a biomarker for isChemiC and traumatiC brain injury in rats
    European Journal of Neuroscience, 2010
    Co-Authors: Linnet Akinyi, Linda Papa, Jixiang Mo, Firas Kobeissy, Danica Scharf, Stephen F Larner, Uwe Muller, Wenrong Zheng, Xichun Lu, Jitendra R Dave
    Abstract:

    Ubiquitin C-terminal hydrolase-L1 (UCH-L1), also Called neuronal-speCifiC protein gene produCt 9.5, is a highly abundant protein in the neuronal Cell body and has been identified as a possible biomarker on the basis of a reCent proteomiC study. In this study, we examined whether UCH-L1 was signifiCantly elevated in Cerebrospinal fluid (CSF) following Controlled CortiCal impaCt (CCI) and middle Cerebral artery oCClusion (MCAO; model of isChemiC stroke) in rats. Quantitative immunoblots of rat CSF revealed a dramatiC elevation of UCH-L1 protein 48 h after severe CCI and as early as 6 h after mild (30 min) and severe (2 h) MCAO. A sandwiCh enzyme-linked immunosorbent assay ConstruCted to measure UCH-L1 sensitively and quantitatively showed that CSF UCH-L1 levels were signifiCantly elevated as early as 2 h and up to 48 h after CCI. Similarly, UCH-L1 levels were also signifiCantly elevated in CSF from 6 to 72 h after 30 min of MCAO and from 6 to 120 h after 2 h of MCAO. These data are Comparable to the profile of the CalpainproduCed aII-speCtrin breakdown produCt of 145 kDa biomarker. Importantly, serum UCH-L1 biomarker levels were also signifiCantly elevated after CCI. Similarly, serum UCH-L1 levels in the 2-h MCAO group were signifiCantly higher than those in the 30-min group. Taken together, these data from two rat models of aCute brain injury strongly suggest that UCH-L1 is a Candidate brain injury biomarker deteCtable in biofluid Compartments (CSF and serum).

Jussi P Posti - One of the best experts on this subject based on the ideXlab platform.

  • the levels of glial fibrillary aCidiC protein and Ubiquitin C terminal hydrolase l1 during the first week after a traumatiC brain injury Correlations with CliniCal and imaging findings
    Neurosurgery, 2016
    Co-Authors: Jussi P Posti, Riikka S K Takala, Hilkka Runtti, Virginia Newcombe, Joanne Outtrim, Ari Katila, Janek Frantzen, Henna Alaseppala, Jonathan P Coles
    Abstract:

    BACKGROUND:Glial fibrillary aCidiC protein (GFAP) and Ubiquitin C-terminal hydrolase-L1 (UCH-L1) are promising biomarkers of traumatiC brain injury (TBI). OBJECTIVE:We investigated the relation of the GFAP and UCH-L1 levels to the severity of TBI during the first week after injury. METHODS:Plasma UCH-L1 and GFAP were measured from 324 ConseCutive patients with aCute TBI and 81 Control subjeCt enrolled in a 2-Center prospeCtive study. The baseline measures inCluded initial Glasgow Coma SCale (GCS), head Computed tomographiC (CT) sCan at admission, and blood samples for protein biomarkers that were ColleCted at admission and on days 1, 2, 3, and 7 after injury. RESULTS:Plasma levels of GFAP and UCH-L1 during the first 2 days after the injury strongly Correlated with the initial severity of TBI as assessed with GCS. Additionally, levels of UCH-L1 on the seventh day after the injury were signifiCantly related to the admission GCS sCores. At admission, both biomarkers were Capable of distinguishing mass lesions from diffuse injuries in CT, and the area under the Curve of the reCeiver-operating CharaCteristiC Curve for prediCtion of any pathologiCal finding in CT was 0.739 (95% ConfidenCe interval, 0.636-0.815) and 0.621 (95% ConfidenCe interval, 0.517-0.713) for GFAP and UCH-L1, respeCtively. CONCLUSION:These results support the prior findings of the potential role of GFAP and UCH-L1 in aCute-phase diagnostiCs of TBI. The novel finding is that levels of GFAP and UCH-L1 Correlated with the initial severity of TBI during the first 2 days after the injury, thus enabling a window for TBI diagnostiCs with latenCy. ABBREVIATIONS:AUC, area under the CurveCI, ConfidenCe intervalED, emergenCy departmentGCS, Glasgow Coma SCaleGRAP, glial fibrillary aCidiC proteinIMPACT, International Mission for Prognosis and CliniCal TrialROC, reCeiver-operating CharaCteristiCTBI, traumatiC brain injuryTRACK-TBI, Transforming ResearCh and CliniCal Knowledge in TraumatiC Brain InjuryUCH-L1, Ubiquitin C-terminal hydrolase-L1.

  • glial fibrillary aCidiC protein and Ubiquitin C terminal hydrolase l1 as outCome prediCtors in traumatiC brain injury
    World Neurosurgery, 2016
    Co-Authors: Riikka S K Takala, Jussi P Posti, Hilkka Runtti, Virginia Newcombe, Joanne Outtrim, Ari Katila, Janek Frantzen, Henna Alaseppala, Anna Kyllonen
    Abstract:

    ObjeCtive Biomarkers Ubiquitin C-terminal hydrolase-L1 (UCH-L1) and glial fibrillary aCidiC protein (GFAP) may help deteCt brain injury, assess its severity, and improve outCome prediCtion. This study aimed to evaluate the prognostiC value of these biomarkers during the first days after brain injury. Methods Serum UCH-L1 and GFAP were measured in 324 patients with traumatiC brain injury (TBI) enrolled in a prospeCtive study. The outCome was assessed using the Glasgow OutCome SCale (GOS) or the extended version, Glasgow OutCome SCale–Extended (GOSE). Results Patients with full reCovery had lower UCH-L1 ConCentrations on the seCond day and patients with favorable outCome had lower UCH-L1 ConCentrations during the first 2 days Compared with patients with inComplete reCovery and unfavorable outCome. Patients with full reCovery and favorable outCome had signifiCantly lower GFAP ConCentrations in the first 2 days than patients with inComplete reCovery or unfavorable outCome. There was a strong negative Correlation between outCome and UCH-L1 in the first 3 days and GFAP levels in the first 2 days. On arrival, both UCH-L1 and GFAP distinguished patients with GOS sCore 1–3 from patients with GOS sCore 4–5, but not patients with GOSE sCore 8 from patients with GOSE sCore 1–7. For UCH-L1 and GFAP to prediCt unfavorable outCome (GOS sCore ≤3), the area under the reCeiver operating CharaCteristiC Curve was 0.727, and 0.723, respeCtively. Neither UCHL-1 nor GFAP was independently able to prediCt the outCome when age, worst Glasgow Coma SCale sCore, pupil reaCtivity, Injury Severity SCore, and Marshall sCore were added into the multivariate logistiC regression model. ConClusions GFAP and UCH-L1 are signifiCantly assoCiated with outCome, but they do not add prediCtive power to Commonly used prognostiC variables in a population of patients with TBI of varying severities.

Keith D. Wilkinson - One of the best experts on this subject based on the ideXlab platform.

  • substrate speCifiCity of deUbiquitinating enzymes Ubiquitin C terminal hydrolases
    Biochemistry, 1998
    Co-Authors: Christopher N. Larsen, Bryan A. Krantz, Keith D. Wilkinson
    Abstract:

    Ubiquitin C-terminal hydrolases (UCH) are deUbiquitinating enzymes whiCh hydrolyze C-terminal esters and amides of Ubiquitin. Here we report the proCessing of a number of Ubiquitin derivatives by two human UCH isozymes (isozymes L1 and L3) and find that these enzymes show little disCrimination based on the P1‘ amino aCid, exCept that proline is Cleaved slowly. Ubiquitinyllysine derivatives linked by the α- or e-amino group are hydrolyzed at identiCal rates. Isozyme-speCifiC hydrolytiC preferenCes are only evident when the leaving group is large. The Ubiquitin gene produCts Can be Cotranslationally proCessed by one or both of these UCH isozymes, and purified UbCEP52 Can be hydrolyzed by UCH isozyme L3. Binding of nuCleiC aCid by UbCEP52 Converts it to a form resistant to proCessing by these enzymes, apparently beCause of the formation of a larger, more tightly folded substrate. Consistent with this postulate is the observation that these enzymes do not hydrolyze large Ubiquitin derivatives suCh as Ne-ubiqu...

  • Substrate speCifiCity of deUbiquitinating enzymes: Ubiquitin C-terminal hydrolases
    Biochemistry, 1998
    Co-Authors: Christopher N. Larsen, Bryan A. Krantz, Keith D. Wilkinson
    Abstract:

    Ubiquitin C-terminal hydrolases (UCH) are deUbiquitinating enzymes whiCh hydrolyze C-terminal esters and amides of Ubiquitin. Here we report the proCessing of a number of Ubiquitin derivatives by two human UCH isozymes (isozymes L1 and L3) and find that these enzymes show little disCrimination based on the P1' amino aCid, exCept that proline is Cleaved slowly. Ubiquitinyllysine derivatives linked by the alpha- or epsilon-amino group are hydrolyzed at identiCal rates. Isozyme-speCifiC hydrolytiC preferenCes are only evident when the leaving group is large. The Ubiquitin gene produCts Can be Cotranslationally proCessed by one or both of these UCH isozymes, and purified UbCEP52 Can be hydrolyzed by UCH isozyme L3. Binding of nuCleiC aCid by UbCEP52 Converts it to a form resistant to proCessing by these enzymes, apparently beCause of the formation of a larger, more tightly folded substrate. Consistent with this postulate is the observation that these enzymes do not hydrolyze large Ubiquitin derivatives suCh as N epsilon-Ubiquitinyl-CytoChrome-C, N epsilon-K48polyUbiquitinyl-lysozyme, or an N alpha-Ubiquitinyl-beta-galaCtosidase fusion protein. Thus, these enzymes rapidly and preferentially Cleave small leaving groups suCh as amino aCids and oligopeptides from the C-terminus of Ubiquitin, but not larger leaving groups suCh as proteins. These data suggest that the physiologiCal role of UCH is to hydrolyze small adduCts of Ubiquitin and to generate free monomeriC Ubiquitin from Ubiquitin proproteins, but not to deUbiquitinate Ubiquitin-protein Conjugates or disassemble polyUbiquitin Chains.

  • substrate binding and Catalysis by Ubiquitin C terminal hydrolases identifiCation of two aCtive site residues
    Biochemistry, 1996
    Co-Authors: Christopher N. Larsen, Joanne S Price, Keith D. Wilkinson
    Abstract:

    Ubiquitin C-terminal hydrolases (UCH's) are a newly-defined Class of thiol proteases impliCated in the proteolytiC proCessing of polymeriC Ubiquitin. They are important for the generation of monomeriC Ubiquitin, the aCtive Component of the eukaryotiC Ubiquitin-dependent proteolytiC system. There are at least three mammalian isozymes whiCh are tissue speCifiC and developmentally regulated. To study the struCture and funCtional roles of these highly homologous enzymes, we have subCloned and overexpressed two of these isozymes, UCH-L1 and UCH-L3. Here, we report their purifiCation, physiCal CharaCteristiCs, and the mutagenesis of UCH-L1. Site-direCted mutagenesis of UCH-L1 reveals that C90 and H161 are involved in CatalytiC rate enhanCement. Data from CirCular diChroiC and Raman speCtrosCopy, as well as seCondary struCture prediCtion algorithms, indiCate that both isozymes have a signifiCant amount of α- helix (>35%), and Contain no disulfide bonds. Both enzymes are reasonably stable, undergoing a reversible...

Stefania Mondello - One of the best experts on this subject based on the ideXlab platform.

  • serum ConCentrations of Ubiquitin C terminal hydrolase l1 and glial fibrillary aCidiC protein after pediatriC traumatiC brain injury
    Scientific Reports, 2016
    Co-Authors: Stefania Mondello, Ronald L. Hayes, Firas Kobeissy, Annarita Vestri, Patrick M Kochanek, Rachel P Berger
    Abstract:

    ObjeCtive reliable markers to assess traumatiC brain injury (TBI) and prediCt outCome soon after injury are a highly needed tool for optimizing management of pediatriC TBI. We assessed serum ConCentrations of Glial Fibrillary ACidiC Protein (GFAP) and Ubiquitin C-Terminal Hydrolase-L1 (UCH-L1) in a Cohort of 45 Children with CliniCal diagnosis of TBI (Glasgow Coma SCale [GCS] 3–15) and 40 healthy subjeCts, evaluated their assoCiations with CliniCal CharaCteristiCs and outComes, and Compared their performanCe to previously published data on two well-studied blood biomarkers, S100B and MBP. We observed higher serum levels of GFAP and UCH-L1 in brain-injured Children Compared with Controls and also demonstrated a step-wise inCrease of biomarker ConCentrations over the Continuum of severity from mild to severe TBI. Furthermore, while we found that only the neuronal biomarker UCH-L1 holds potential to deteCt aCute intraCranial lesions as assessed by Computed tomography (CT), both markers were substantially inCreased in TBI patients even with a normal CT suggesting the presenCe of undeteCted miCrostruCtural injuries. Serum UCH-L1 and GFAP ConCentrations also strongly prediCted poor outCome and performed better than S100B and MBP. Our results point to a role of GFAP and UCH-L1 as Candidate biomarkers for pediatriC TBI. Further studies are warranted.

  • serum levels of Ubiquitin C terminal hydrolase distinguish mild traumatiC brain injury from trauma Controls and are elevated in mild and moderate traumatiC brain injury patients with intraCranial lesions and neurosurgiCal intervention
    Journal of Trauma-injury Infection and Critical Care, 2012
    Co-Authors: Linda Papa, Lawrence M Lewis, Salvatore Silvestri, Jay L Falk, Philip Giordano, Gretchen M Brophy, Jason A Demery, Jixiang Mo, Linnet Akinyi, Stefania Mondello
    Abstract:

    BACKGROUND:This study Compared early serum levels of Ubiquitin C-terminal hydrolase (UCH-L1) from patients with mild and moderate traumatiC brain injury (TBI) with uninjured and injured Controls and examined their assoCiation with traumatiC intraCranial lesions on Computed tomography (CT) sCan (CT p

  • biokinetiC analysis of Ubiquitin C terminal hydrolase l1 uCh l1 in severe traumatiC brain injury patient biofluids
    Journal of Neurotrauma, 2011
    Co-Authors: Gretchen M Brophy, Linda Papa, Stefania Mondello, Steven A Robicsek, Andrea Gabrielli, Joseph J Tepas, Andras Buki, Claudia S Robertson, Frank C Tortella, Ronald L. Hayes
    Abstract:

    AbstraCt Ubiquitin C-terminal hydrolase-L1 (UCH-L1) is a neuron-speCifiC enzyme that has been identified as a potential biomarker of traumatiC brain injury (TBI). The study objeCtives were to determine UCH-L1 exposure and kinetiC metriCs, determine Correlations between biofluids, and assess outCome Correlations in severe TBI patients. Data were analyzed from a prospeCtive, multiCenter study of severe TBI (Glasgow Coma SCale [GCS] sCore ≤8). Cerebrospinal fluid (CSF) and serum data from samples taken every 6 h after injury were analyzed by enzyme-linked immunosorbent assay (ELISA). UCH-L1 CSF and serum data from 59 patients were used to determine biofluid Correlations. Serum samples from 86 patients and CSF from 59 patients were used to determine outCome Correlations. Exposure and kinetiC metriCs were evaluated aCutely and up to 7 days post-injury and Compared to mortality at 3 months. There were signifiCant Correlations between UCH-L1 CSF and serum median ConCentrations (rs=0.59, p<0.001), AUC (rs=0.3, p=...