The Experts below are selected from a list of 1863 Experts worldwide ranked by ideXlab platform
Dani Eshel - One of the best experts on this subject based on the ideXlab platform.
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vacuolar processing enzyme activates programmed cell death in the apical meriStem inducing loss of apical dominance
Plant Cell and Environment, 2017Co-Authors: Paula Teperbamnolker, Yossi Buskila, Amnon Lers, Eduard Belausov, Dalia Wolf, Adi Doronfaigenboim, Shifra Bendor, Renier A L Van Der Hoorn, Dani EshelAbstract:The potato (Solanum tuberosum L.) tuber is a swollen Underground Stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (StVPE) in the tuber apical meriStem (TAM). VPE activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 protein (mVPE) exhibits specific activity for caspase-1, but not caspase-3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNAi resulted in reduced Stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to GFP showed enhanced Stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and Stem branching.
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stronger sink demand for metabolites supports dominance of the apical bud in etiolated growth
Journal of Experimental Botany, 2016Co-Authors: Yossi Buskila, Noa Sela, Paula Teperbamnolker, Eilon Shani, Roy Weinstain, Victor Gaba, Amnon Lers, Dani EshelAbstract:: The potato tuber is a swollen Underground Stem that can sprout under dark conditions. Sprouting initiates in the tuber apical bud (AP), while lateral buds (LTs) are repressed by apical dominance (AD). Under conditions of lost AD, removal of tuber LTs showed that they partially inhibit AP growth only at the AD stage. Detached buds were inhibited by exogenous application of naphthaleneacetic acid (NAA), whereas 6-benzyladenine (6-BA) and gibberellic acid (GA3) induced bud burst and elongation, respectively. NAA, applied after 6-BA or GA3, nullified the latters' growth-stimulating effect in both the AP and LTs. GA3 applied to the fifth-position LT was transported mainly to the tuber's AP. GA3 treatment also resulted in increased indole-3-acetic acid (IAA) concentration and cis-zeatin O-glucoside in the AP. In a tuber tissue strip that included two or three buds connected by the peripheral vascular syStem, treatment of a LT with GA3 affected only the AP side of the strip, suggesting that the AP is the strongest sink for GA3, which induces its etiolated elongation. Dipping etiolated sprouts in labeled GA3 showed specific accumulation of the signal in the AP. Transcriptome analysis of GA3's effect showed that genes related to the cell cycle, cell proliferation, and hormone transport are up-regulated in the AP as compared to the LT. Sink demand for metabolites is suggested to support AD in etiolated Stem growth by inducing differential gene expression in the AP.
Yossi Buskila - One of the best experts on this subject based on the ideXlab platform.
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vacuolar processing enzyme activates programmed cell death in the apical meriStem inducing loss of apical dominance
Plant Cell and Environment, 2017Co-Authors: Paula Teperbamnolker, Yossi Buskila, Amnon Lers, Eduard Belausov, Dalia Wolf, Adi Doronfaigenboim, Shifra Bendor, Renier A L Van Der Hoorn, Dani EshelAbstract:The potato (Solanum tuberosum L.) tuber is a swollen Underground Stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (StVPE) in the tuber apical meriStem (TAM). VPE activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 protein (mVPE) exhibits specific activity for caspase-1, but not caspase-3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNAi resulted in reduced Stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to GFP showed enhanced Stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and Stem branching.
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stronger sink demand for metabolites supports dominance of the apical bud in etiolated growth
Journal of Experimental Botany, 2016Co-Authors: Yossi Buskila, Noa Sela, Paula Teperbamnolker, Eilon Shani, Roy Weinstain, Victor Gaba, Amnon Lers, Dani EshelAbstract:: The potato tuber is a swollen Underground Stem that can sprout under dark conditions. Sprouting initiates in the tuber apical bud (AP), while lateral buds (LTs) are repressed by apical dominance (AD). Under conditions of lost AD, removal of tuber LTs showed that they partially inhibit AP growth only at the AD stage. Detached buds were inhibited by exogenous application of naphthaleneacetic acid (NAA), whereas 6-benzyladenine (6-BA) and gibberellic acid (GA3) induced bud burst and elongation, respectively. NAA, applied after 6-BA or GA3, nullified the latters' growth-stimulating effect in both the AP and LTs. GA3 applied to the fifth-position LT was transported mainly to the tuber's AP. GA3 treatment also resulted in increased indole-3-acetic acid (IAA) concentration and cis-zeatin O-glucoside in the AP. In a tuber tissue strip that included two or three buds connected by the peripheral vascular syStem, treatment of a LT with GA3 affected only the AP side of the strip, suggesting that the AP is the strongest sink for GA3, which induces its etiolated elongation. Dipping etiolated sprouts in labeled GA3 showed specific accumulation of the signal in the AP. Transcriptome analysis of GA3's effect showed that genes related to the cell cycle, cell proliferation, and hormone transport are up-regulated in the AP as compared to the LT. Sink demand for metabolites is suggested to support AD in etiolated Stem growth by inducing differential gene expression in the AP.
Paula Teperbamnolker - One of the best experts on this subject based on the ideXlab platform.
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vacuolar processing enzyme activates programmed cell death in the apical meriStem inducing loss of apical dominance
Plant Cell and Environment, 2017Co-Authors: Paula Teperbamnolker, Yossi Buskila, Amnon Lers, Eduard Belausov, Dalia Wolf, Adi Doronfaigenboim, Shifra Bendor, Renier A L Van Der Hoorn, Dani EshelAbstract:The potato (Solanum tuberosum L.) tuber is a swollen Underground Stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (StVPE) in the tuber apical meriStem (TAM). VPE activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 protein (mVPE) exhibits specific activity for caspase-1, but not caspase-3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNAi resulted in reduced Stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to GFP showed enhanced Stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and Stem branching.
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stronger sink demand for metabolites supports dominance of the apical bud in etiolated growth
Journal of Experimental Botany, 2016Co-Authors: Yossi Buskila, Noa Sela, Paula Teperbamnolker, Eilon Shani, Roy Weinstain, Victor Gaba, Amnon Lers, Dani EshelAbstract:: The potato tuber is a swollen Underground Stem that can sprout under dark conditions. Sprouting initiates in the tuber apical bud (AP), while lateral buds (LTs) are repressed by apical dominance (AD). Under conditions of lost AD, removal of tuber LTs showed that they partially inhibit AP growth only at the AD stage. Detached buds were inhibited by exogenous application of naphthaleneacetic acid (NAA), whereas 6-benzyladenine (6-BA) and gibberellic acid (GA3) induced bud burst and elongation, respectively. NAA, applied after 6-BA or GA3, nullified the latters' growth-stimulating effect in both the AP and LTs. GA3 applied to the fifth-position LT was transported mainly to the tuber's AP. GA3 treatment also resulted in increased indole-3-acetic acid (IAA) concentration and cis-zeatin O-glucoside in the AP. In a tuber tissue strip that included two or three buds connected by the peripheral vascular syStem, treatment of a LT with GA3 affected only the AP side of the strip, suggesting that the AP is the strongest sink for GA3, which induces its etiolated elongation. Dipping etiolated sprouts in labeled GA3 showed specific accumulation of the signal in the AP. Transcriptome analysis of GA3's effect showed that genes related to the cell cycle, cell proliferation, and hormone transport are up-regulated in the AP as compared to the LT. Sink demand for metabolites is suggested to support AD in etiolated Stem growth by inducing differential gene expression in the AP.
Amnon Lers - One of the best experts on this subject based on the ideXlab platform.
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vacuolar processing enzyme activates programmed cell death in the apical meriStem inducing loss of apical dominance
Plant Cell and Environment, 2017Co-Authors: Paula Teperbamnolker, Yossi Buskila, Amnon Lers, Eduard Belausov, Dalia Wolf, Adi Doronfaigenboim, Shifra Bendor, Renier A L Van Der Hoorn, Dani EshelAbstract:The potato (Solanum tuberosum L.) tuber is a swollen Underground Stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (StVPE) in the tuber apical meriStem (TAM). VPE activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 protein (mVPE) exhibits specific activity for caspase-1, but not caspase-3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNAi resulted in reduced Stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to GFP showed enhanced Stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and Stem branching.
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stronger sink demand for metabolites supports dominance of the apical bud in etiolated growth
Journal of Experimental Botany, 2016Co-Authors: Yossi Buskila, Noa Sela, Paula Teperbamnolker, Eilon Shani, Roy Weinstain, Victor Gaba, Amnon Lers, Dani EshelAbstract:: The potato tuber is a swollen Underground Stem that can sprout under dark conditions. Sprouting initiates in the tuber apical bud (AP), while lateral buds (LTs) are repressed by apical dominance (AD). Under conditions of lost AD, removal of tuber LTs showed that they partially inhibit AP growth only at the AD stage. Detached buds were inhibited by exogenous application of naphthaleneacetic acid (NAA), whereas 6-benzyladenine (6-BA) and gibberellic acid (GA3) induced bud burst and elongation, respectively. NAA, applied after 6-BA or GA3, nullified the latters' growth-stimulating effect in both the AP and LTs. GA3 applied to the fifth-position LT was transported mainly to the tuber's AP. GA3 treatment also resulted in increased indole-3-acetic acid (IAA) concentration and cis-zeatin O-glucoside in the AP. In a tuber tissue strip that included two or three buds connected by the peripheral vascular syStem, treatment of a LT with GA3 affected only the AP side of the strip, suggesting that the AP is the strongest sink for GA3, which induces its etiolated elongation. Dipping etiolated sprouts in labeled GA3 showed specific accumulation of the signal in the AP. Transcriptome analysis of GA3's effect showed that genes related to the cell cycle, cell proliferation, and hormone transport are up-regulated in the AP as compared to the LT. Sink demand for metabolites is suggested to support AD in etiolated Stem growth by inducing differential gene expression in the AP.
Joao Maximo De Siqueira - One of the best experts on this subject based on the ideXlab platform.
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chemical composition and evaluation of the anti inflammatory and antinociceptive activities of duguetia furfuracea essential oil effect on edema leukocyte recruitment tumor necrosis factor alpha production inos expression and adenosinergic and opioidergic syStems
Journal of Ethnopharmacology, 2019Co-Authors: Aline Aparecida Saldanha, Leticia Vieira, Rosy Iara Maciel De Azambuja Ribeiro, Ralph Gruppi Thome, Helio Batista Dos Santos, Denise Brentan Silva, Carlos Alexandre Carollo, Flavio Martins De Oliveira, Debora De Oliveira Lopes, Joao Maximo De SiqueiraAbstract:Abstract Ethnopharmacological relevance Duguetia furfuracea (A. St. -Hil.) Saff. (Annonaceae) is commonly known in Brazil as “araticum-seco,” and its root is used in folk medicine to treat inflammatory and painful disorders. However, no studies have been performed to evaluate these therapeutic activities. Aim of the study Investigate the chemical composition, anti-inflammatory and antinociceptive effects, and elucidate the possible antinociceptive mechanisms of action from the essential oil of D. furfuracea (EODf) Underground Stem bark. Materials and methods Chemical composition was determined by gas chromatography and mass spectrometry (GC/MS). The paw edema induced by LPS, formalin-induced nociception, LPS-induced thermal hyperalgesia and rota-rod tests in vivo were used to evaluate the anti-inflammatory and antinociceptive effects in addition to the alteration on motor coordination. Histological analyses and an immunohistochemistry assay for iNOS were performed on mouse footpads of naive, control, 10 mg/kg EODf, and 10 mg/kg indomethacin (Ind) groups. The samples were removed at 1, 3, and 6 h after subplantar injection of LPS. In addition, the involvement of the adenosinergic, opioidergic, serotonergic, and cholinergic syStems were investigated, in order to elucidate possible antinociceptive mechanisms. Results Twenty-four volatile constituents were detected and identified. (E)-asarone (21.9%), bicyclogermacrene (16.7%), 2,4,5-trimethoxystyrene (16.1%), α-gurjunene (15%), cyperene (7.8%), and (E)-caryophyllene (4.6%) were major compounds found in EODf. Oral treatment (p.o.) with EODf (1, 3, and 10 mg/kg) significantly inhibited the paw edema induced by LPS. At 10 mg/kg EODf promoted inhibition of tumor necrosis factor alpha (TNF-α) production, recruitment of polymorphonuclear (PMN) leukocytes and inducible nitric oxide synthase (iNOS) expression in paw tissue. EODf (10 and 30 mg/kg, p.o.) also reduced licking time in both phases of the formalin test and it had a significant effect on the LPS-induced thermal hyperalgesia model. The administration of caffeine (Caf) and naloxone (Nal) reversed the antinociceptive activity of EODf, in the first phase of the formalin test and in the LPS-induced thermal hyperalgesia model. Moreover, Nal was also able to abolish the antinociception caused by EODf, in the second phase of formalin test. In the rota-rod test, EODf-treated animals did not show any alteration of motor coordination. Conclusions Our findings indicate that EODf Underground Stem bark produces anti-inflammatory and both central and peripheral antinociceptive effects. Furthermore, the antinociceptive activity of EODf Underground Stem bark is possibly mediated by adenosinergic and opioidergic pathways, and its properties do not induce effects on motor coordination. These results support the use of the folk medicine, D. furfuracea root, to treat inflammation and painful conditions.
