The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform
F. P. Guengerich - One of the best experts on this subject based on the ideXlab platform.
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Metabolic Reactions: Types of Reactions of Cytochrome P450 Enzymes
Handbook of experimental pharmacology, 1993Co-Authors: F. P. GuengerichAbstract:Collectively the cytochrome P450 enzymes (EC 1.14.14.1, Unspecific Monooxygenase) catalyze thousands of different reactions. Even when allowance is made for the similarity of different substrates the number of reactions is still considerable (Fig. 1). However, these can be considered in terms of a few groups of oxidative reactions that proceed through similar chemistry. Most of the oxidative reactions have been demonstrated with biomimetic chemical models, and it is thought that the protein structure determines the catalytic specificity through complementarity to the transition state. The possibility that specific amino acid residues participate directly in chemical events (e.g., specific acid-base catalysis) has not been considered in depth - indeed, even the function of the axial thiolate ligand is not precisely known (Dawson 1988; Imai et al. 1989). The biomimetic model oxidations are not dependent upon the presence of a particular metal, ligand structure, or solvent. Indeed, much has been inferred about the catalytic mechanism of the enzyme from studies with these models (Mansuy et al. 1989) because some of the postulated enzyme intermediates are probably inaccessible for direct observation.
Rui Hua Jiao - One of the best experts on this subject based on the ideXlab platform.
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Dalmanol biosyntheses require coupling of two separate polyketide gene clusters.
Chemical Science, 2018Co-Authors: Zhen Zhen Zhou, Xuan Zhang, Hui Ming Ge, Rui Hua JiaoAbstract:Polyketide–polyketide hybrids are unique natural products with promising bioactivity, but the hybridization processes remain poorly understood. Herein, we present that the biosynthetic pathways of two immunosuppressants, dalmanol A and acetodalmanol A, result from an Unspecific Monooxygenase triggered hybridization of two distinct polyketide (naphthalene and chromane) biosynthetic gene clusters. The orchestration of the functional dimorphism of the polyketide synthase (ChrA) ketoreductase (KR) domain (shortened as ChrA KR) with that of the KR partner (ChrB) in the bioassembly line increases the polyketide diversity and allows the fungal generation of plant chromanes (e.g., noreugenin) and phloroglucinols (e.g., 2,4,6-trihydroxyacetophenone). The simultaneous fungal biosynthesis of 1,3,6,8- and 2-acetyl-1,3,6,8-tetrahydroxynaphthalenes was addressed as well. Collectively, the work may symbolize a movement in understanding the multiple-gene-cluster involved natural product biosynthesis, and highlights the possible fungal generations of some chromane- and phloroglucinol-based phytochemicals.
Zhen Zhen Zhou - One of the best experts on this subject based on the ideXlab platform.
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Dalmanol biosyntheses require coupling of two separate polyketide gene clusters.
Chemical Science, 2018Co-Authors: Zhen Zhen Zhou, Xuan Zhang, Hui Ming Ge, Rui Hua JiaoAbstract:Polyketide–polyketide hybrids are unique natural products with promising bioactivity, but the hybridization processes remain poorly understood. Herein, we present that the biosynthetic pathways of two immunosuppressants, dalmanol A and acetodalmanol A, result from an Unspecific Monooxygenase triggered hybridization of two distinct polyketide (naphthalene and chromane) biosynthetic gene clusters. The orchestration of the functional dimorphism of the polyketide synthase (ChrA) ketoreductase (KR) domain (shortened as ChrA KR) with that of the KR partner (ChrB) in the bioassembly line increases the polyketide diversity and allows the fungal generation of plant chromanes (e.g., noreugenin) and phloroglucinols (e.g., 2,4,6-trihydroxyacetophenone). The simultaneous fungal biosynthesis of 1,3,6,8- and 2-acetyl-1,3,6,8-tetrahydroxynaphthalenes was addressed as well. Collectively, the work may symbolize a movement in understanding the multiple-gene-cluster involved natural product biosynthesis, and highlights the possible fungal generations of some chromane- and phloroglucinol-based phytochemicals.
Xuan Zhang - One of the best experts on this subject based on the ideXlab platform.
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Dalmanol biosyntheses require coupling of two separate polyketide gene clusters.
Chemical Science, 2018Co-Authors: Zhen Zhen Zhou, Xuan Zhang, Hui Ming Ge, Rui Hua JiaoAbstract:Polyketide–polyketide hybrids are unique natural products with promising bioactivity, but the hybridization processes remain poorly understood. Herein, we present that the biosynthetic pathways of two immunosuppressants, dalmanol A and acetodalmanol A, result from an Unspecific Monooxygenase triggered hybridization of two distinct polyketide (naphthalene and chromane) biosynthetic gene clusters. The orchestration of the functional dimorphism of the polyketide synthase (ChrA) ketoreductase (KR) domain (shortened as ChrA KR) with that of the KR partner (ChrB) in the bioassembly line increases the polyketide diversity and allows the fungal generation of plant chromanes (e.g., noreugenin) and phloroglucinols (e.g., 2,4,6-trihydroxyacetophenone). The simultaneous fungal biosynthesis of 1,3,6,8- and 2-acetyl-1,3,6,8-tetrahydroxynaphthalenes was addressed as well. Collectively, the work may symbolize a movement in understanding the multiple-gene-cluster involved natural product biosynthesis, and highlights the possible fungal generations of some chromane- and phloroglucinol-based phytochemicals.
Hui Ming Ge - One of the best experts on this subject based on the ideXlab platform.
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Dalmanol biosyntheses require coupling of two separate polyketide gene clusters.
Chemical Science, 2018Co-Authors: Zhen Zhen Zhou, Xuan Zhang, Hui Ming Ge, Rui Hua JiaoAbstract:Polyketide–polyketide hybrids are unique natural products with promising bioactivity, but the hybridization processes remain poorly understood. Herein, we present that the biosynthetic pathways of two immunosuppressants, dalmanol A and acetodalmanol A, result from an Unspecific Monooxygenase triggered hybridization of two distinct polyketide (naphthalene and chromane) biosynthetic gene clusters. The orchestration of the functional dimorphism of the polyketide synthase (ChrA) ketoreductase (KR) domain (shortened as ChrA KR) with that of the KR partner (ChrB) in the bioassembly line increases the polyketide diversity and allows the fungal generation of plant chromanes (e.g., noreugenin) and phloroglucinols (e.g., 2,4,6-trihydroxyacetophenone). The simultaneous fungal biosynthesis of 1,3,6,8- and 2-acetyl-1,3,6,8-tetrahydroxynaphthalenes was addressed as well. Collectively, the work may symbolize a movement in understanding the multiple-gene-cluster involved natural product biosynthesis, and highlights the possible fungal generations of some chromane- and phloroglucinol-based phytochemicals.
