The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Lei Cui - One of the best experts on this subject based on the ideXlab platform.
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Reconstruction of three-dimensional neoUrethra using lingual keratinocytes and corporal smooth muscle cells seeded acellular corporal spongiosum.
Tissue engineering. Part A, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) "neoUrethra" tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 months. Simple epithelial layer regenerated in group B while there was no evidence of CSMCs growing into grafts during study period. Stratified epithelial layer and organized muscle fiber bundles were evident after 6 months in group C. Our study suggested that lingual keratinocytes and CSMCs could be used as a source of seed cells for Urethral tissue engineering. Using the dynamic-static seeding method, 3D Urethra could be constructed in vivo.
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20 RECONSTRUCTION OF THREE DIMENSIONAL NEO-Urethra USING LINGUAL KERATINOCYTES & CORPORAL SMOOTH MUSCLE CELLS SEEDED ACELLULAR CORPORAL SPONGIOSUM
The Journal of Urology, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) “neoUrethra” tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 mon...
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20 reconstruction of three dimensional neo Urethra using lingual keratinocytes corporal smooth muscle cells seeded acellular corporal spongiosum
The Journal of Urology, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) “neoUrethra” tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 mon...
Chao Feng - One of the best experts on this subject based on the ideXlab platform.
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Reconstruction of three-dimensional neoUrethra using lingual keratinocytes and corporal smooth muscle cells seeded acellular corporal spongiosum.
Tissue engineering. Part A, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) "neoUrethra" tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 months. Simple epithelial layer regenerated in group B while there was no evidence of CSMCs growing into grafts during study period. Stratified epithelial layer and organized muscle fiber bundles were evident after 6 months in group C. Our study suggested that lingual keratinocytes and CSMCs could be used as a source of seed cells for Urethral tissue engineering. Using the dynamic-static seeding method, 3D Urethra could be constructed in vivo.
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20 RECONSTRUCTION OF THREE DIMENSIONAL NEO-Urethra USING LINGUAL KERATINOCYTES & CORPORAL SMOOTH MUSCLE CELLS SEEDED ACELLULAR CORPORAL SPONGIOSUM
The Journal of Urology, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) “neoUrethra” tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 mon...
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20 reconstruction of three dimensional neo Urethra using lingual keratinocytes corporal smooth muscle cells seeded acellular corporal spongiosum
The Journal of Urology, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) “neoUrethra” tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 mon...
Wei-dong Zhu - One of the best experts on this subject based on the ideXlab platform.
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Reconstruction of three-dimensional neoUrethra using lingual keratinocytes and corporal smooth muscle cells seeded acellular corporal spongiosum.
Tissue engineering. Part A, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) "neoUrethra" tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 months. Simple epithelial layer regenerated in group B while there was no evidence of CSMCs growing into grafts during study period. Stratified epithelial layer and organized muscle fiber bundles were evident after 6 months in group C. Our study suggested that lingual keratinocytes and CSMCs could be used as a source of seed cells for Urethral tissue engineering. Using the dynamic-static seeding method, 3D Urethra could be constructed in vivo.
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20 RECONSTRUCTION OF THREE DIMENSIONAL NEO-Urethra USING LINGUAL KERATINOCYTES & CORPORAL SMOOTH MUSCLE CELLS SEEDED ACELLULAR CORPORAL SPONGIOSUM
The Journal of Urology, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) “neoUrethra” tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 mon...
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20 reconstruction of three dimensional neo Urethra using lingual keratinocytes corporal smooth muscle cells seeded acellular corporal spongiosum
The Journal of Urology, 2011Co-Authors: Chao Feng, Wei-dong Zhu, Lei CuiAbstract:Tissue engineering technique seems to be the best strategy for treating the Urethra Disease in the near future. In present study, porcine acellular corpus spongiosum matrices (ACSMs) were combined with autologus corporal smooth muscle cells (CSMCs) and lingual keratinocytes by static-dynamic seeding method to form three-dimensional (3D) “neoUrethra” tissue for repairing the Urethral defection. Six scaffolds with two kinds of cells (Group C), six scaffolds with only lingual keratinocytes (Group B), and six matrices alone (Group A) were used to repair the rabbit Urethral defection. Retrograde urethrography and histological analyses were performed to evaluate the results of urethroplasty. In vitro, hematoxylin and eosin staining of seeded ACSM showed multiple epithelial layers and well-distributed CSMCs into the matrix. In vivo, the Urethra kept a wide caliber in Group C. Strictures were observed in groups A and B. Histologically, the retrieved Urethra in group A showed fibrosis and inflammation during 6 mon...
Su-mi Shin - One of the best experts on this subject based on the ideXlab platform.
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Bladder and Urethra Disease
Radiology Illustrated: Pediatric Radiology, 2013Co-Authors: Su-mi ShinAbstract:Congenital anomalies of the lower urinary tract may cause a significant morbidity in infants and children. This chapter discusses and illustrates a wide spectrum of abnormalities of the bladder and Urethra. These abnormalities include urachal anomalies, bladder exstrophy, bladder diverticula, cystitis, bladder neoplasms, neurogenic bladder, posterior Urethral valves, Urethral duplication, and prostatic utricle. Brief embryologic review is also included.
Jiang Xuemei - One of the best experts on this subject based on the ideXlab platform.
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The study of the male Urethra in the quiescent period with ultrasound oxygen retrograde urethrography
Chinese Journal of Uitrasonography, 2001Co-Authors: Jiang XuemeiAbstract:Objective To study the form of the normal and abnormal male Urethra in quiescent period and its clinical significance and to evaluate the clinical application of ultrasound oxygen retrograde urethrography(UORU) in Urethra Disease.Methods Eight normal young men(group Ⅰ), 1 patient with Urethra cancer and 28 cases with benign prostatic hypertrophy(group Ⅱ) were examined by UORU.Results The Urethras in the two groups could not be observed before UORU,but they were all observed after UORU.In groupⅠ,the normal male Urethras in the quiescent period were smooth,glossy and bright lines and the tracks of the posterior Urethras were flat.In groupⅡ,the gas in the Urethra cancer was dispersed,just as the flowers that the fairy lady had scattered here and there.The posterior Urethras of the patients with benign prostatic hypertrophy were all lengthened over 3 cm,the tracks in the quiescent period were almost abnormal (27/28),some of the radians of posterior Urethra were enlarged or some changed into tortuousness.Conclusions The form of the male Urethra in the quiescent period can be completely displayed by UORU,UORU is helpful to analyse the integrality of mucous membrane,it is a new,simple and useful method for the diagnosis of the male Urethra Disease.
