The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform
Karen A Holbrook - One of the best experts on this subject based on the ideXlab platform.
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analysis of skin derived amniotic fluid cells in the second trimester detection of severe genodermatoses expressed in the fetal period
Journal of Investigative Dermatology, 1994Co-Authors: M Akiyama, Karen A HolbrookAbstract:Abstract Amniotic fluid contains skin-derived cells. To determine whether populations of amniotic fluid cells reflect genetic conditions, we surveyed the population of amniotic fluid cells from 36 fetuses at 16–21 weeks estimated gestational age at risk for junctional epidermolysis bullosa, recessive dystrophic epidermolysis bullosa, epidermolysis bullosa Dowling-Meara, harlequin ichthyosis, lamellar ichthyosis (nonbullous congenital ichthyosiform erythroderma), or Sjogren-Larson syndrome. Periderm cells, keratinocytes, cells of unknown epithelial origin, fibroblasts, fibrin clots, and Urinary Cast-like materials were seen in the amniotic fluid from normal fetuses. A large number of small macrophages phagocytizing collagen fibers was found in the amniotic fluid from all recessive dystrophic epidermolysis bullosa, some junctional epidermolysis bullosa fetuses, and a single epidermolysis bullosa Dowling-Meara fetus. Immuno-gold electron microscopy with anti-lysozyme and anti-CD68 antibodies confirmed that the cells morphologically identified as macrophages were active macrophages containing many lysosomes. The percentage of macrophages/total amniotic fluid cells in amniotic fluid samples from the fetuses affected with recessive dystrophic epidermolysis bullosa was significantly increased (12.30 ± 0.20%) compared with that in the amniotic fluid from normal fetuses (1.30 ± 0.65%) (p
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Analysis of skin-derived amniotic fluid cells in the second trimester; detection of severe genodermatoses expressed in the fetal period.
The Journal of investigative dermatology, 1994Co-Authors: M Akiyama, Karen A HolbrookAbstract:Amniotic fluid contains skin-derived cells. To determine whether populations of amniotic fluid cells reflect genetic conditions, we surveyed the population of amniotic fluid cells from 36 fetuses at 16-21 weeks estimated gestational age at risk for junctional epidermolysis bullosa, recessive dystrophic epidermolysis bullosa, epidermolysis bullosa Dowling-Meara, harlequin ichthyosis, lamellar ichthyosis (nonbullous congenital ichthyosiform erythroderma), or Sjogren-Larson syndrome. Periderm cells, keratinocytes, cells of unknown epithelial origin, fibroblasts, fibrin clots, and Urinary Cast-like materials were seen in the amniotic fluid from normal fetuses. A large number of small macrophages phagocytizing collagen fibers was found in the amniotic fluid from all recessive dystrophic epidermolysis bullosa, some junctional epidermolysis bullosa fetuses, and a single epidermolysis bullosa Dowling-Meara fetus. Immuno-gold electron microscopy with anti-lysozyme and anti-CD68 antibodies confirmed that the cells morphologically identified as macrophages were active macrophages containing many lysosomes. The percentage of macrophages/total amniotic fluid cells in amniotic fluid samples from the fetuses affected with recessive dystrophic epidermolysis bullosa was significantly increased (12.30 +/- 0.20%) compared with that in the amniotic fluid from normal fetuses (1.30 +/- 0.65%) (p < 0.001). In amniotic fluid samples from fetuses affected with harlequin ichthyosis and lamellar ichthyosis, clumps of keratinized cells that contained disease-specific abnormal granules and lipid droplets were observed. These results indicate that the morphologic analysis of amniotic fluid cells can provide important supportive information for the prenatal diagnosis of several severe genodermatoses.
M Akiyama - One of the best experts on this subject based on the ideXlab platform.
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analysis of skin derived amniotic fluid cells in the second trimester detection of severe genodermatoses expressed in the fetal period
Journal of Investigative Dermatology, 1994Co-Authors: M Akiyama, Karen A HolbrookAbstract:Abstract Amniotic fluid contains skin-derived cells. To determine whether populations of amniotic fluid cells reflect genetic conditions, we surveyed the population of amniotic fluid cells from 36 fetuses at 16–21 weeks estimated gestational age at risk for junctional epidermolysis bullosa, recessive dystrophic epidermolysis bullosa, epidermolysis bullosa Dowling-Meara, harlequin ichthyosis, lamellar ichthyosis (nonbullous congenital ichthyosiform erythroderma), or Sjogren-Larson syndrome. Periderm cells, keratinocytes, cells of unknown epithelial origin, fibroblasts, fibrin clots, and Urinary Cast-like materials were seen in the amniotic fluid from normal fetuses. A large number of small macrophages phagocytizing collagen fibers was found in the amniotic fluid from all recessive dystrophic epidermolysis bullosa, some junctional epidermolysis bullosa fetuses, and a single epidermolysis bullosa Dowling-Meara fetus. Immuno-gold electron microscopy with anti-lysozyme and anti-CD68 antibodies confirmed that the cells morphologically identified as macrophages were active macrophages containing many lysosomes. The percentage of macrophages/total amniotic fluid cells in amniotic fluid samples from the fetuses affected with recessive dystrophic epidermolysis bullosa was significantly increased (12.30 ± 0.20%) compared with that in the amniotic fluid from normal fetuses (1.30 ± 0.65%) (p
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Analysis of skin-derived amniotic fluid cells in the second trimester; detection of severe genodermatoses expressed in the fetal period.
The Journal of investigative dermatology, 1994Co-Authors: M Akiyama, Karen A HolbrookAbstract:Amniotic fluid contains skin-derived cells. To determine whether populations of amniotic fluid cells reflect genetic conditions, we surveyed the population of amniotic fluid cells from 36 fetuses at 16-21 weeks estimated gestational age at risk for junctional epidermolysis bullosa, recessive dystrophic epidermolysis bullosa, epidermolysis bullosa Dowling-Meara, harlequin ichthyosis, lamellar ichthyosis (nonbullous congenital ichthyosiform erythroderma), or Sjogren-Larson syndrome. Periderm cells, keratinocytes, cells of unknown epithelial origin, fibroblasts, fibrin clots, and Urinary Cast-like materials were seen in the amniotic fluid from normal fetuses. A large number of small macrophages phagocytizing collagen fibers was found in the amniotic fluid from all recessive dystrophic epidermolysis bullosa, some junctional epidermolysis bullosa fetuses, and a single epidermolysis bullosa Dowling-Meara fetus. Immuno-gold electron microscopy with anti-lysozyme and anti-CD68 antibodies confirmed that the cells morphologically identified as macrophages were active macrophages containing many lysosomes. The percentage of macrophages/total amniotic fluid cells in amniotic fluid samples from the fetuses affected with recessive dystrophic epidermolysis bullosa was significantly increased (12.30 +/- 0.20%) compared with that in the amniotic fluid from normal fetuses (1.30 +/- 0.65%) (p < 0.001). In amniotic fluid samples from fetuses affected with harlequin ichthyosis and lamellar ichthyosis, clumps of keratinized cells that contained disease-specific abnormal granules and lipid droplets were observed. These results indicate that the morphologic analysis of amniotic fluid cells can provide important supportive information for the prenatal diagnosis of several severe genodermatoses.
Alessandro C. Pasqualotto - One of the best experts on this subject based on the ideXlab platform.
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Cryptococcus within a Urinary Cast
Kidney international, 2013Co-Authors: José Antonio Tesser Poloni, Liane Nanci Rotta, Carlos Franco Voegeli, Alessandro C. PasqualottoAbstract:A 13-year-old girl with AIDS was admitted to the hospital with neck pain and vomiting. Routine urinalysis revealed an unusual Cast containing two encapsulated budding yeast forms entrapped within the matrix (Figure 1). The urine sediment was stained with China ink and revealed free encapsulated budding yeasts (Figure 2). The morphology of the yeast was consistent with Cryptococcus sp. The fungus was also isolated in the cerebrospinal fluid (CSF) and in liver and bone marrow biopsy, characterizing disseminated cryptococcosis. The latex agglutination test was positive in the serum (titer 1:4096). The patient is currently on treatment for the cryptococcal infection with amphotericin B. The diagnosis of disseminated cryptococcosis is usually made by CSF analysis. Urine is an easy-to-obtain sample that may be useful in a search for Cryptococcus sp. Although reports describing cryptococcuria exist, this is the first report of Cryptococcus sp. being entrapped within a Urinary Cast. Urine microscopy and culture could provide important clues to the presence of cryptococcal infection. n e p h r o l o g y i m a g e http://www.kidney-international.org
José Antonio Tesser Poloni - One of the best experts on this subject based on the ideXlab platform.
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Cryptococcus within a Urinary Cast
Kidney international, 2013Co-Authors: José Antonio Tesser Poloni, Liane Nanci Rotta, Carlos Franco Voegeli, Alessandro C. PasqualottoAbstract:A 13-year-old girl with AIDS was admitted to the hospital with neck pain and vomiting. Routine urinalysis revealed an unusual Cast containing two encapsulated budding yeast forms entrapped within the matrix (Figure 1). The urine sediment was stained with China ink and revealed free encapsulated budding yeasts (Figure 2). The morphology of the yeast was consistent with Cryptococcus sp. The fungus was also isolated in the cerebrospinal fluid (CSF) and in liver and bone marrow biopsy, characterizing disseminated cryptococcosis. The latex agglutination test was positive in the serum (titer 1:4096). The patient is currently on treatment for the cryptococcal infection with amphotericin B. The diagnosis of disseminated cryptococcosis is usually made by CSF analysis. Urine is an easy-to-obtain sample that may be useful in a search for Cryptococcus sp. Although reports describing cryptococcuria exist, this is the first report of Cryptococcus sp. being entrapped within a Urinary Cast. Urine microscopy and culture could provide important clues to the presence of cryptococcal infection. n e p h r o l o g y i m a g e http://www.kidney-international.org
Hideaki Yoshino - One of the best experts on this subject based on the ideXlab platform.
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Urinary Cast is a useful predictor of acute kidney injury in acute heart failure
Scientific reports, 2019Co-Authors: Satoshi Higuchi, Yusuke Kabeya, Kenichi Matsushita, Satoko Yamasaki, Hiroaki Ohnishi, Hideaki YoshinoAbstract:Acute kidney injury (AKI) is associated with poor prognosis among patients with acute heart failure (AHF). Early documentation of impaired kidney function through simple examination may provide risk reduction in such patients. The present study aims to reveal an association between cellular Casts and hospital-acquired AKI in AHF. This study included patients with AHF who underwent urinalysis, including Urinary sediment analysis within 24 hours post admission. AKI was defined as an increase of ≥0.3 mg/dL within 48 hours or ≥1.5 times in serum creatinine level in contrast to baseline creatinine level. In this study, 114 patients with AHF (age, 75 ± 14 years; male, 59.7%) were included. Of them, 40 (35%) developed hospital-acquired AKI. Cellular Casts were detected in 30 patients (26%) prior to AKI development and related to hospital-acquired AKI in the multivariate logistic regression analysis (odds ratio, 2.80; 95% confidence interval, 1.04–7.49; P = 0.041). In conclusion, cellular Casts are observed occasionally in patients with AHF and potentially useful markers for development of AKI during hospitalization.
