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Klaus Fischer - One of the best experts on this subject based on the ideXlab platform.
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simultaneous and sensitive hplc determination of mono and disaccharides Uronic Acids and amino sugars after derivatization by reductive amination
Acta Hydrochimica Et Hydrobiologica, 2003Co-Authors: Klaus Fischer, Marion Wacht, Axel MeyerAbstract:The reductive amination of low-molecular-weight saccharides, Uronic Acids, and amino sugars, followed by a separation of the derivates by means of ion-pair chromatography or RP-HPLC, offers an interesting alternative to HPAEC-PAD for the environmental analysis of these compounds. Under this aspect various potential amination reagents, i.e., p-amino-benzoic acid (p-AMBA), p-AMBA propyl ester, 1-aminopyrene, 2-(2-aminophenyl)indole, and 4-aminoazobenzene, were tested with regard to the formation of derivates and to the chromatographic properties of the formed derivates. p-AMBA, p-AMBA propyl ester and 4-aminoazobenzene proved to be especially suited, because they facilitate the amination of all carbohydrate reference components together with a complete separation and sensible detection (detection limits < 0.5 mg/L) of the derivates. Mainly the following elution sequence was ascertained: amino sugars (hexosamines) / disaccharide(s) / monosaccharides (hexoses) / hexUronic acid(s) / N-acetyl-D-glucosamine. Detection limits down to 0.1 μmol/L were realized using p-AMBA as reagent, facilitating the determination of the target compounds in landfill leachates and lysimeter percolates. Applying the p-AMBA propyl ester for derivatization, chromatographic interferences with weakly retained derivates and the coelution of the reagent with its galactosamine derivate can be avoided, since the ester elutes after its derivates unlike p-AMBA itself. Simultane und sensitive HPLC-Bestimmung von Mono- und Disacchariden, Uronsauren und Aminozuckern nach Derivatisierung mittels reduktiver Aminierung Die reduktive Aminierung von niedermolekularen Sacchariden, Uronsauren und Aminozuckern, kombiniert mit ionenpaarchromatographischer oder RP-HPLC-Trennung der Derivate, eroffnet eine interessante Alternative in der Umweltanalytik dieser Verbindungen gegenuber der HPAEC-PAD. Vor diesem Hintergrund wurden einige potentielle Aminierungsreagentien, u.a. p-Aminobenzoesaure (p-AMBA), der p-AMBA-Propylester, 1-Aminopyren, 2-(2-Aminophenyl)-indol und 4-Aminoazobenzol, hinsichtlich der Derivatbildung und der chromatographischen Eigenschaften der Derivate getestet. Dabei erwiesen sich p-AMBA, ihr Propylester und 4-Aminoazobenzol als besonders geeignet, da mit ihnen eine Derivatisierung samtlicher Kohlenhydratreferenzkomponenten sowie eine vollstandige chromatographische Auftrennung der Derivate und ihre empfindliche Detektion (NWG < 0.5 mg/L) moglich ist. In der Regel wurde folgende Elutionssequenz erhalten: Aminozucker (Hexosamine) / Disaccharide / Monosaccharide (Hexosen) / Hexuronsaure(n) / N-Acetyl-D-glucosamin. Mit p-AMBA wurden Nachweisgrenzen von bis zu 0.1 μmol/L erzielt, was die Bestimmung der Zielkomponenten in Deponiesickerwassern und Lysimeterperkolaten erlaubte. Bei Verwendung des p-AMBA-Propylesters, der im Unterschied zur p-AMBA nach den Derivaten eluiert, konnen Interferenzen mit wenig retardierten Derivaten und die Coelution mit Galactosamin vermieden werden.
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ion pair rp hplc determination of sugars amino sugars and Uronic Acids after derivatization with p aminobenzoic acid
Analytical Chemistry, 2001Co-Authors: Axel Meyer, Christine Raba, Klaus FischerAbstract:A new, selective, and sensitive ion-pair RP-HPLC method for the simultaneous determination of three classes of natural organic compounds, i.e., carbohydrates, amino sugars, and Uronic Acids, in env...
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ion pair rp hplc determination of sugars amino sugars and Uronic Acids after derivatization with p aminobenzoic acid
Analytical Chemistry, 2001Co-Authors: Axel Meyer, Christine Raba, Klaus FischerAbstract:A new, selective, and sensitive ion-pair RP-HPLC method for the simultaneous determination of three classes of natural organic compounds, i.e., carbohydrates, amino sugars, and Uronic Acids, in environmental samples is presented. p-Aminobenzoic acid is used for precolumn derivatization of the analytes, enabling fluorescence (lambda(ex) 313 nm, lambda(em) 358 nm) or photometric detection (303 nm). The dependence of the derivatization yield on the reaction conditions is examined. Derivatives of lactose, galactose, glucose, mannose, xylose, arabinose, galactUronic acid, glucUronic acid, N-acetylglucosamine, and glycerinealdehyde were separated on a RP-C18 column with hydrophilic end capping within 35 min, applying TBAHSO4 as the ion-pair reagent. The concentration detection limits range between 20 and 30 microg L(-1) ((1-2) x 10(-7) M) for fluorescence detection and between 30 and 75 microg L(-1) for UV detection. A good linearity is achieved in the concentration range from 50 microg L(-1) to 100 mg L(-1) (r2 > 0.99). The described method has been applied for the determination of mono-/disaccharides, Uronic Acids, and amino sugars in soil solutions and in landfill leachates.
Axel Meyer - One of the best experts on this subject based on the ideXlab platform.
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simultaneous and sensitive hplc determination of mono and disaccharides Uronic Acids and amino sugars after derivatization by reductive amination
Acta Hydrochimica Et Hydrobiologica, 2003Co-Authors: Klaus Fischer, Marion Wacht, Axel MeyerAbstract:The reductive amination of low-molecular-weight saccharides, Uronic Acids, and amino sugars, followed by a separation of the derivates by means of ion-pair chromatography or RP-HPLC, offers an interesting alternative to HPAEC-PAD for the environmental analysis of these compounds. Under this aspect various potential amination reagents, i.e., p-amino-benzoic acid (p-AMBA), p-AMBA propyl ester, 1-aminopyrene, 2-(2-aminophenyl)indole, and 4-aminoazobenzene, were tested with regard to the formation of derivates and to the chromatographic properties of the formed derivates. p-AMBA, p-AMBA propyl ester and 4-aminoazobenzene proved to be especially suited, because they facilitate the amination of all carbohydrate reference components together with a complete separation and sensible detection (detection limits < 0.5 mg/L) of the derivates. Mainly the following elution sequence was ascertained: amino sugars (hexosamines) / disaccharide(s) / monosaccharides (hexoses) / hexUronic acid(s) / N-acetyl-D-glucosamine. Detection limits down to 0.1 μmol/L were realized using p-AMBA as reagent, facilitating the determination of the target compounds in landfill leachates and lysimeter percolates. Applying the p-AMBA propyl ester for derivatization, chromatographic interferences with weakly retained derivates and the coelution of the reagent with its galactosamine derivate can be avoided, since the ester elutes after its derivates unlike p-AMBA itself. Simultane und sensitive HPLC-Bestimmung von Mono- und Disacchariden, Uronsauren und Aminozuckern nach Derivatisierung mittels reduktiver Aminierung Die reduktive Aminierung von niedermolekularen Sacchariden, Uronsauren und Aminozuckern, kombiniert mit ionenpaarchromatographischer oder RP-HPLC-Trennung der Derivate, eroffnet eine interessante Alternative in der Umweltanalytik dieser Verbindungen gegenuber der HPAEC-PAD. Vor diesem Hintergrund wurden einige potentielle Aminierungsreagentien, u.a. p-Aminobenzoesaure (p-AMBA), der p-AMBA-Propylester, 1-Aminopyren, 2-(2-Aminophenyl)-indol und 4-Aminoazobenzol, hinsichtlich der Derivatbildung und der chromatographischen Eigenschaften der Derivate getestet. Dabei erwiesen sich p-AMBA, ihr Propylester und 4-Aminoazobenzol als besonders geeignet, da mit ihnen eine Derivatisierung samtlicher Kohlenhydratreferenzkomponenten sowie eine vollstandige chromatographische Auftrennung der Derivate und ihre empfindliche Detektion (NWG < 0.5 mg/L) moglich ist. In der Regel wurde folgende Elutionssequenz erhalten: Aminozucker (Hexosamine) / Disaccharide / Monosaccharide (Hexosen) / Hexuronsaure(n) / N-Acetyl-D-glucosamin. Mit p-AMBA wurden Nachweisgrenzen von bis zu 0.1 μmol/L erzielt, was die Bestimmung der Zielkomponenten in Deponiesickerwassern und Lysimeterperkolaten erlaubte. Bei Verwendung des p-AMBA-Propylesters, der im Unterschied zur p-AMBA nach den Derivaten eluiert, konnen Interferenzen mit wenig retardierten Derivaten und die Coelution mit Galactosamin vermieden werden.
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ion pair rp hplc determination of sugars amino sugars and Uronic Acids after derivatization with p aminobenzoic acid
Analytical Chemistry, 2001Co-Authors: Axel Meyer, Christine Raba, Klaus FischerAbstract:A new, selective, and sensitive ion-pair RP-HPLC method for the simultaneous determination of three classes of natural organic compounds, i.e., carbohydrates, amino sugars, and Uronic Acids, in env...
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ion pair rp hplc determination of sugars amino sugars and Uronic Acids after derivatization with p aminobenzoic acid
Analytical Chemistry, 2001Co-Authors: Axel Meyer, Christine Raba, Klaus FischerAbstract:A new, selective, and sensitive ion-pair RP-HPLC method for the simultaneous determination of three classes of natural organic compounds, i.e., carbohydrates, amino sugars, and Uronic Acids, in environmental samples is presented. p-Aminobenzoic acid is used for precolumn derivatization of the analytes, enabling fluorescence (lambda(ex) 313 nm, lambda(em) 358 nm) or photometric detection (303 nm). The dependence of the derivatization yield on the reaction conditions is examined. Derivatives of lactose, galactose, glucose, mannose, xylose, arabinose, galactUronic acid, glucUronic acid, N-acetylglucosamine, and glycerinealdehyde were separated on a RP-C18 column with hydrophilic end capping within 35 min, applying TBAHSO4 as the ion-pair reagent. The concentration detection limits range between 20 and 30 microg L(-1) ((1-2) x 10(-7) M) for fluorescence detection and between 30 and 75 microg L(-1) for UV detection. A good linearity is achieved in the concentration range from 50 microg L(-1) to 100 mg L(-1) (r2 > 0.99). The described method has been applied for the determination of mono-/disaccharides, Uronic Acids, and amino sugars in soil solutions and in landfill leachates.
Christine Raba - One of the best experts on this subject based on the ideXlab platform.
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ion pair rp hplc determination of sugars amino sugars and Uronic Acids after derivatization with p aminobenzoic acid
Analytical Chemistry, 2001Co-Authors: Axel Meyer, Christine Raba, Klaus FischerAbstract:A new, selective, and sensitive ion-pair RP-HPLC method for the simultaneous determination of three classes of natural organic compounds, i.e., carbohydrates, amino sugars, and Uronic Acids, in env...
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ion pair rp hplc determination of sugars amino sugars and Uronic Acids after derivatization with p aminobenzoic acid
Analytical Chemistry, 2001Co-Authors: Axel Meyer, Christine Raba, Klaus FischerAbstract:A new, selective, and sensitive ion-pair RP-HPLC method for the simultaneous determination of three classes of natural organic compounds, i.e., carbohydrates, amino sugars, and Uronic Acids, in environmental samples is presented. p-Aminobenzoic acid is used for precolumn derivatization of the analytes, enabling fluorescence (lambda(ex) 313 nm, lambda(em) 358 nm) or photometric detection (303 nm). The dependence of the derivatization yield on the reaction conditions is examined. Derivatives of lactose, galactose, glucose, mannose, xylose, arabinose, galactUronic acid, glucUronic acid, N-acetylglucosamine, and glycerinealdehyde were separated on a RP-C18 column with hydrophilic end capping within 35 min, applying TBAHSO4 as the ion-pair reagent. The concentration detection limits range between 20 and 30 microg L(-1) ((1-2) x 10(-7) M) for fluorescence detection and between 30 and 75 microg L(-1) for UV detection. A good linearity is achieved in the concentration range from 50 microg L(-1) to 100 mg L(-1) (r2 > 0.99). The described method has been applied for the determination of mono-/disaccharides, Uronic Acids, and amino sugars in soil solutions and in landfill leachates.
Xingyao Xiong - One of the best experts on this subject based on the ideXlab platform.
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quantification of sugar compounds and Uronic Acids in enzymatic hydrolysates of lignocellulose using high performance anion exchange chromatography with pulsed amperometric detection
Energy & Fuels, 2012Co-Authors: Lin Tan, Zhou Heng, Tingting Zhang, Ziwei Jiang, Xingyao XiongAbstract:A method was developed for simultaneous determination of seven sugar compounds and two Uronic Acids by high-performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) using a CarboPac PA20 column under a gradient elution (mobile phase: H2O/200 mM NaOH/100 mM NaAc) at 0.4 mL/min. Under these conditions, d-(−)-arabinose, d-(+)-galactose, d-(+)-glucose, d-(+)-mannose, d-(+)-xylose, d-(−)-fructose, d-(+)-cellobiose, d-galactUronic acid, and d-glucUronic acid were all separated in 46 min. The calibration curve, detection limits, reproducibility and accuracy were also determined. Furthermore, seven sugar compounds and two Uronic Acids were all detected in the enzymatic hydrolysates of different lignocellulosic materials treated by different pretreatment processes. Our results demonstrate that this method was feasible for sugar compounds separation without the need for derivatization and organic solvent, and it also has the advantages of having low interference, high sensitivity, ...
Christina M Szabo - One of the best experts on this subject based on the ideXlab platform.
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complete monosaccharide analysis by high performance anion exchange chromatography with pulsed amperometric detection
Analytical Chemistry, 2012Co-Authors: Zhenqing Zhang, Nazeer M Khan, Karen M Nunez, Edward K Chess, Christina M SzaboAbstract:Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including Uronic Acids and sialic Acids, are also important components in some complex carbohydrates. In this report, a high-performance anion-exchange chromatography method with pulsed amperometric detection was initially developed to analyze acidic sugars including different Uronic Acids and sialic Acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method has a limit of quantitation of ∼12.5 × 10–3 nmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety of complex carbohyd...
