The Experts below are selected from a list of 5346 Experts worldwide ranked by ideXlab platform
Behzad Baradaran - One of the best experts on this subject based on the ideXlab platform.
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APOPTOTIC EFFECT OF THE Urtica Dioica PLANT EXTRACTS ON BREAST CANCER CELL LINE (MDA- MB- 468)
2020Co-Authors: Ali Mohammadi, Behzad BaradaranAbstract:Background & objectives: Cancer is one of the most causes of mortality in worldwide. Components derived from natural plants that induce apoptosis are used for cancer treatment. Therefore investigation of different herbal components for new anti-cancer drug is one of the main research activities throughout the world. According to low cost, oral consumption and easy access to the public extracts of Urtica Dioica, in this study we aimed to investigate the effectiveness of this herb on MDA-MB-468 breast cancer cells. Methods: Cytotoxic effect of Urtica Dioica extract was measured using MTT assays. To show induction of apoptosis by this plant TUNEL and DNA Fragmentation test were performed. Results: In the present study dichloromethane extracts noticeably killed cancer cells. IC50 values related to human breast adenocarcinoma cell line MDA-MB-468 were 29.46±1.05 µg/ml in 24 hours and 15.54±1.04 µg/ml in 48 hours. TUNEL test and DNA Fragmentation assay showed apoptotic characteristic in the extract treated cells. Conclusion: The results showed that MDA-MB-468 cells after treatment with dichloromethane extract of Urtica Dioica, induces apoptosis in MDA -MB-468 cancer cells which may be useful in the treatment of canc er.
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the Urtica Dioica extract enhances sensitivity of paclitaxel drug to mda mb 468 breast cancer cells
Biomedicine & Pharmacotherapy, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Mahyar Aghapour, Solmaz Shirjang, Sanam Nami, Behzad BaradaranAbstract:Abstract Introduction Due to the chemo resistant nature of cancer cells and adverse effects of current therapies, researchers are looking for the most efficient therapeutic approach which has the lowest side effects and the highest toxicity on cancer cells. The aim of the present study was to investigate the synergic effect of Urtica Dioica extract in combination with paclitaxel on cell death and invasion of human breast cancer MDA-MB-468 cell line. Materials and methods To determine the cytotoxic effects of Urtica Dioica extract with paclitaxel, MTT assay was performed. The scratch test was exploited to assess the effects of Urtica Dioica, Paclitaxel alone and combination on migration of cancer cells. The expression levels of snail-1, ZEB1, ZEB2, twist, Cdc2, cyclin B1 and Wee1 genes were quantified using qRT-PCR and western blot performed for snail-1expression. The effects of plant extract, Paclitaxel alone and combination on different phases of cell cycle was analyzed using flow cytometry. Results Results of MTT assay showed that Urtica Dioica significantly destroyed cancer cells. Interestingly, Concurrent use of Urtica Dioica extract with paclitaxel resulted in decreased IC50 dose of paclitaxel. Moreover, findings of scratch assay exhibited the inhibitory effects of Urtica Dioica, Paclitaxel alone and combination on migration of MDA-MB-468 cell line. Our findings also demonstrated that the extract substantially decreased the Snail-1 and related gene expression. Ultimately, Cell cycle arrest occurred at G2/M phase post-treatment by deregulating Cdc2 and wee1. Conclusions Our results demonstrated that the dichloromethane extract of Urtica Dioica inhibit cell growth and migration. Also, Urtica Dioica extract substantially increased sensitivity of breast cancer cells to paclitaxel. Therefore, it can be used as a potential candidate for treatment of breast cancer with paclitaxel.
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Urtica Dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells pc3
Cellular and Molecular Biology, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Mahyar Aghapour, Behzad BaradaranAbstract:Prostate cancer is considered as the major cause of death among men around the world. There are a number of medicinal plants triggering apoptosis response in cancer cells, thus have a therapeutic potential. Therefore, further studies to characterize beneficial properties of these plants in order to introduce novel anti-cancer drugs are the interest of recent researches on the alternative medicine. On the other hand, due to traditional uses and availability of Urtica Dioica extract, we decided to evaluate the efficacy of this medicinal herb on pc3 prostate cancer cell line. In the present study the cytotoxic effects of Urtica Dioica extract were assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and trypan blue viability dye. Then, DNA fragmentation and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were exploited to measure cell death and apoptosis stage. The expression levels of caspase 3, caspase 9 and Bcl-2 genes were quantified by Real-Time PCR. Finally, Cell cycle was analyzed by flow cytometry. MTT assay showed that dichloromethanolic extract of Urtica Dioica significantly inhibited the cell growth. According to the DNA fragmentation and TUNEL assay results, the herbal extract was able to induce apoptosis in prostate cancer cells. Our findings also demonstrated that the plant extract substantially increases the caspase 3 and 9 mRNA expression, while decreases Bcl-2. Cell cycle arrest was occurred in G2 stage, due to the results of flow cytometry. These results indicate that dichloromethanolic extract of Urtica Dioica can successfully induce apoptosis in PC3 cells. Therefore, it could be used as a novel therapeutic candidate for prostate tumor treatment.
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effects of Urtica Dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line mda mb 468
Cellular and Molecular Biology, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Samira Goldar, Dariush Shanehbandi, Vahid Khaze, Leila Mohammadnejad, Elham Baghbani, Behzad BaradaranAbstract:Breast cancer is the most common cancer among women in worldwide, especially in developing countries. Therefore, a large number of anticancer agents with herbal origins have been reported against this deadly disease. This study is the first to examine the cytotoxic and apoptotic effects of Urtica Dioica in MDA-MB-468, human breast adenocarcinoma cells. The 3-(4,5-dimethylethiazol-2 yl)-2,5- diphenyltetrazolium (MTT) reduction and trypan-blue exclusion assay were performed in MDA-MB-468 cells as well as control cell line L929 to analyze the cytotoxic activity of the dichloromethane extract. In addition, Apoptosis induction of Urtica Dioica on the MDA-MB-468 cells was assessed using TUNEL (terminal deoxy transferase (TdT)-mediated dUTP nick- end labeling) assay and DNA fragmentation analysis and real-time polymerase chain reaction (PCR). The results showed that the extract significantly inhibited cell growth and viability without inducing damage to normal control cells. Nuclei Staining in TUNEL and DNA fragments in DNA fragmentation assay and increase in the mRNA expression levels of caspase-3, caspase-9, decrease in the bcl2 and no significant change in the caspase-8 mRNA expression level, showed that the induction of apoptosis was the main mechanism of cell death that induce by Urtica Dioica extract. Our results suggest that Urtica Dioica dichloromethane extract may contain potential bioactive compound(s) for the treatment of breast adenocarcinoma.
Ali Mohammadi - One of the best experts on this subject based on the ideXlab platform.
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APOPTOTIC EFFECT OF THE Urtica Dioica PLANT EXTRACTS ON BREAST CANCER CELL LINE (MDA- MB- 468)
2020Co-Authors: Ali Mohammadi, Behzad BaradaranAbstract:Background & objectives: Cancer is one of the most causes of mortality in worldwide. Components derived from natural plants that induce apoptosis are used for cancer treatment. Therefore investigation of different herbal components for new anti-cancer drug is one of the main research activities throughout the world. According to low cost, oral consumption and easy access to the public extracts of Urtica Dioica, in this study we aimed to investigate the effectiveness of this herb on MDA-MB-468 breast cancer cells. Methods: Cytotoxic effect of Urtica Dioica extract was measured using MTT assays. To show induction of apoptosis by this plant TUNEL and DNA Fragmentation test were performed. Results: In the present study dichloromethane extracts noticeably killed cancer cells. IC50 values related to human breast adenocarcinoma cell line MDA-MB-468 were 29.46±1.05 µg/ml in 24 hours and 15.54±1.04 µg/ml in 48 hours. TUNEL test and DNA Fragmentation assay showed apoptotic characteristic in the extract treated cells. Conclusion: The results showed that MDA-MB-468 cells after treatment with dichloromethane extract of Urtica Dioica, induces apoptosis in MDA -MB-468 cancer cells which may be useful in the treatment of canc er.
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the Urtica Dioica extract enhances sensitivity of paclitaxel drug to mda mb 468 breast cancer cells
Biomedicine & Pharmacotherapy, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Mahyar Aghapour, Solmaz Shirjang, Sanam Nami, Behzad BaradaranAbstract:Abstract Introduction Due to the chemo resistant nature of cancer cells and adverse effects of current therapies, researchers are looking for the most efficient therapeutic approach which has the lowest side effects and the highest toxicity on cancer cells. The aim of the present study was to investigate the synergic effect of Urtica Dioica extract in combination with paclitaxel on cell death and invasion of human breast cancer MDA-MB-468 cell line. Materials and methods To determine the cytotoxic effects of Urtica Dioica extract with paclitaxel, MTT assay was performed. The scratch test was exploited to assess the effects of Urtica Dioica, Paclitaxel alone and combination on migration of cancer cells. The expression levels of snail-1, ZEB1, ZEB2, twist, Cdc2, cyclin B1 and Wee1 genes were quantified using qRT-PCR and western blot performed for snail-1expression. The effects of plant extract, Paclitaxel alone and combination on different phases of cell cycle was analyzed using flow cytometry. Results Results of MTT assay showed that Urtica Dioica significantly destroyed cancer cells. Interestingly, Concurrent use of Urtica Dioica extract with paclitaxel resulted in decreased IC50 dose of paclitaxel. Moreover, findings of scratch assay exhibited the inhibitory effects of Urtica Dioica, Paclitaxel alone and combination on migration of MDA-MB-468 cell line. Our findings also demonstrated that the extract substantially decreased the Snail-1 and related gene expression. Ultimately, Cell cycle arrest occurred at G2/M phase post-treatment by deregulating Cdc2 and wee1. Conclusions Our results demonstrated that the dichloromethane extract of Urtica Dioica inhibit cell growth and migration. Also, Urtica Dioica extract substantially increased sensitivity of breast cancer cells to paclitaxel. Therefore, it can be used as a potential candidate for treatment of breast cancer with paclitaxel.
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Urtica Dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells pc3
Cellular and Molecular Biology, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Mahyar Aghapour, Behzad BaradaranAbstract:Prostate cancer is considered as the major cause of death among men around the world. There are a number of medicinal plants triggering apoptosis response in cancer cells, thus have a therapeutic potential. Therefore, further studies to characterize beneficial properties of these plants in order to introduce novel anti-cancer drugs are the interest of recent researches on the alternative medicine. On the other hand, due to traditional uses and availability of Urtica Dioica extract, we decided to evaluate the efficacy of this medicinal herb on pc3 prostate cancer cell line. In the present study the cytotoxic effects of Urtica Dioica extract were assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and trypan blue viability dye. Then, DNA fragmentation and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were exploited to measure cell death and apoptosis stage. The expression levels of caspase 3, caspase 9 and Bcl-2 genes were quantified by Real-Time PCR. Finally, Cell cycle was analyzed by flow cytometry. MTT assay showed that dichloromethanolic extract of Urtica Dioica significantly inhibited the cell growth. According to the DNA fragmentation and TUNEL assay results, the herbal extract was able to induce apoptosis in prostate cancer cells. Our findings also demonstrated that the plant extract substantially increases the caspase 3 and 9 mRNA expression, while decreases Bcl-2. Cell cycle arrest was occurred in G2 stage, due to the results of flow cytometry. These results indicate that dichloromethanolic extract of Urtica Dioica can successfully induce apoptosis in PC3 cells. Therefore, it could be used as a novel therapeutic candidate for prostate tumor treatment.
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effects of Urtica Dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line mda mb 468
Cellular and Molecular Biology, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Samira Goldar, Dariush Shanehbandi, Vahid Khaze, Leila Mohammadnejad, Elham Baghbani, Behzad BaradaranAbstract:Breast cancer is the most common cancer among women in worldwide, especially in developing countries. Therefore, a large number of anticancer agents with herbal origins have been reported against this deadly disease. This study is the first to examine the cytotoxic and apoptotic effects of Urtica Dioica in MDA-MB-468, human breast adenocarcinoma cells. The 3-(4,5-dimethylethiazol-2 yl)-2,5- diphenyltetrazolium (MTT) reduction and trypan-blue exclusion assay were performed in MDA-MB-468 cells as well as control cell line L929 to analyze the cytotoxic activity of the dichloromethane extract. In addition, Apoptosis induction of Urtica Dioica on the MDA-MB-468 cells was assessed using TUNEL (terminal deoxy transferase (TdT)-mediated dUTP nick- end labeling) assay and DNA fragmentation analysis and real-time polymerase chain reaction (PCR). The results showed that the extract significantly inhibited cell growth and viability without inducing damage to normal control cells. Nuclei Staining in TUNEL and DNA fragments in DNA fragmentation assay and increase in the mRNA expression levels of caspase-3, caspase-9, decrease in the bcl2 and no significant change in the caspase-8 mRNA expression level, showed that the induction of apoptosis was the main mechanism of cell death that induce by Urtica Dioica extract. Our results suggest that Urtica Dioica dichloromethane extract may contain potential bioactive compound(s) for the treatment of breast adenocarcinoma.
Haleh Akhavanniaki - One of the best experts on this subject based on the ideXlab platform.
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antioxidant and apoptotic effects of an aqueous extract of Urtica Dioica on the mcf 7 human breast cancer cell line
Asian Pacific Journal of Cancer Prevention, 2013Co-Authors: Sadegh Fattahi, Ali M Ardekani, Ebrahim Zabihi, Zeinab Abedian, Amrollah Mostafazadeh, Roghayeh Pourbagher, Haleh AkhavanniakiAbstract:Breast cancer is the most prevalent cancer and one of the leading causes of death among women in the world. Plants and herbs may play an important role in complementary or alternative treatment. The aim of this study was to evaluate the antioxidant and anti-proliferative potential of Urtica Dioica. The anti oxidant activity of an aqueous extract of Urtica Dioica leaf was measured by MTT assay and the FRAP method while its anti-proliferative activity on the human breast cancer cell line (MCF-7) and fibroblasts isolated from foreskin tissue was evaluated using MTT assay. Mechanisms leading to apoptosis were also investigated at the molecular level by measuring the amount of anti and pro-apoptotic proteins and at the cellular level by studying DNA fragmentation and annexin V staining by flow cytometry. The aqueous extract of Urtica Dioica showed antioxidant effects with a correlation coefficient of r 2 =0.997. Dose-dependent and anti-proliferative effects of the extract were observed only on MCF-7 cells after 72 hrs with an IC 50 value of 2 mg/ml. This anti proliferative activity was associated with an increase of apoptosis as demonstrated by DNA fragmentation, the appearance of apoptotic cells in flow cytometry analysis and an increase of the amount of calpain 1, calpastatin, caspase 3, caspase 9, Bax and Bcl-2, all proteins involved in the apoptotic pathway. This is the first time such in vitro antiproliferative effect of aqueous extract of Urtica Dioica leaf has been described for a breast cancer cell line. Our findings warrant further research on Urtica Dioica as a potential chemotherapeutic agent for breast cancer.
Behzad Mansoori - One of the best experts on this subject based on the ideXlab platform.
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the Urtica Dioica extract enhances sensitivity of paclitaxel drug to mda mb 468 breast cancer cells
Biomedicine & Pharmacotherapy, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Mahyar Aghapour, Solmaz Shirjang, Sanam Nami, Behzad BaradaranAbstract:Abstract Introduction Due to the chemo resistant nature of cancer cells and adverse effects of current therapies, researchers are looking for the most efficient therapeutic approach which has the lowest side effects and the highest toxicity on cancer cells. The aim of the present study was to investigate the synergic effect of Urtica Dioica extract in combination with paclitaxel on cell death and invasion of human breast cancer MDA-MB-468 cell line. Materials and methods To determine the cytotoxic effects of Urtica Dioica extract with paclitaxel, MTT assay was performed. The scratch test was exploited to assess the effects of Urtica Dioica, Paclitaxel alone and combination on migration of cancer cells. The expression levels of snail-1, ZEB1, ZEB2, twist, Cdc2, cyclin B1 and Wee1 genes were quantified using qRT-PCR and western blot performed for snail-1expression. The effects of plant extract, Paclitaxel alone and combination on different phases of cell cycle was analyzed using flow cytometry. Results Results of MTT assay showed that Urtica Dioica significantly destroyed cancer cells. Interestingly, Concurrent use of Urtica Dioica extract with paclitaxel resulted in decreased IC50 dose of paclitaxel. Moreover, findings of scratch assay exhibited the inhibitory effects of Urtica Dioica, Paclitaxel alone and combination on migration of MDA-MB-468 cell line. Our findings also demonstrated that the extract substantially decreased the Snail-1 and related gene expression. Ultimately, Cell cycle arrest occurred at G2/M phase post-treatment by deregulating Cdc2 and wee1. Conclusions Our results demonstrated that the dichloromethane extract of Urtica Dioica inhibit cell growth and migration. Also, Urtica Dioica extract substantially increased sensitivity of breast cancer cells to paclitaxel. Therefore, it can be used as a potential candidate for treatment of breast cancer with paclitaxel.
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Urtica Dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells pc3
Cellular and Molecular Biology, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Mahyar Aghapour, Behzad BaradaranAbstract:Prostate cancer is considered as the major cause of death among men around the world. There are a number of medicinal plants triggering apoptosis response in cancer cells, thus have a therapeutic potential. Therefore, further studies to characterize beneficial properties of these plants in order to introduce novel anti-cancer drugs are the interest of recent researches on the alternative medicine. On the other hand, due to traditional uses and availability of Urtica Dioica extract, we decided to evaluate the efficacy of this medicinal herb on pc3 prostate cancer cell line. In the present study the cytotoxic effects of Urtica Dioica extract were assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and trypan blue viability dye. Then, DNA fragmentation and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were exploited to measure cell death and apoptosis stage. The expression levels of caspase 3, caspase 9 and Bcl-2 genes were quantified by Real-Time PCR. Finally, Cell cycle was analyzed by flow cytometry. MTT assay showed that dichloromethanolic extract of Urtica Dioica significantly inhibited the cell growth. According to the DNA fragmentation and TUNEL assay results, the herbal extract was able to induce apoptosis in prostate cancer cells. Our findings also demonstrated that the plant extract substantially increases the caspase 3 and 9 mRNA expression, while decreases Bcl-2. Cell cycle arrest was occurred in G2 stage, due to the results of flow cytometry. These results indicate that dichloromethanolic extract of Urtica Dioica can successfully induce apoptosis in PC3 cells. Therefore, it could be used as a novel therapeutic candidate for prostate tumor treatment.
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effects of Urtica Dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line mda mb 468
Cellular and Molecular Biology, 2016Co-Authors: Ali Mohammadi, Behzad Mansoori, Samira Goldar, Dariush Shanehbandi, Vahid Khaze, Leila Mohammadnejad, Elham Baghbani, Behzad BaradaranAbstract:Breast cancer is the most common cancer among women in worldwide, especially in developing countries. Therefore, a large number of anticancer agents with herbal origins have been reported against this deadly disease. This study is the first to examine the cytotoxic and apoptotic effects of Urtica Dioica in MDA-MB-468, human breast adenocarcinoma cells. The 3-(4,5-dimethylethiazol-2 yl)-2,5- diphenyltetrazolium (MTT) reduction and trypan-blue exclusion assay were performed in MDA-MB-468 cells as well as control cell line L929 to analyze the cytotoxic activity of the dichloromethane extract. In addition, Apoptosis induction of Urtica Dioica on the MDA-MB-468 cells was assessed using TUNEL (terminal deoxy transferase (TdT)-mediated dUTP nick- end labeling) assay and DNA fragmentation analysis and real-time polymerase chain reaction (PCR). The results showed that the extract significantly inhibited cell growth and viability without inducing damage to normal control cells. Nuclei Staining in TUNEL and DNA fragments in DNA fragmentation assay and increase in the mRNA expression levels of caspase-3, caspase-9, decrease in the bcl2 and no significant change in the caspase-8 mRNA expression level, showed that the induction of apoptosis was the main mechanism of cell death that induce by Urtica Dioica extract. Our results suggest that Urtica Dioica dichloromethane extract may contain potential bioactive compound(s) for the treatment of breast adenocarcinoma.
Sadegh Fattahi - One of the best experts on this subject based on the ideXlab platform.
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antioxidant and apoptotic effects of an aqueous extract of Urtica Dioica on the mcf 7 human breast cancer cell line
Asian Pacific Journal of Cancer Prevention, 2013Co-Authors: Sadegh Fattahi, Ali M Ardekani, Ebrahim Zabihi, Zeinab Abedian, Amrollah Mostafazadeh, Roghayeh Pourbagher, Haleh AkhavanniakiAbstract:Breast cancer is the most prevalent cancer and one of the leading causes of death among women in the world. Plants and herbs may play an important role in complementary or alternative treatment. The aim of this study was to evaluate the antioxidant and anti-proliferative potential of Urtica Dioica. The anti oxidant activity of an aqueous extract of Urtica Dioica leaf was measured by MTT assay and the FRAP method while its anti-proliferative activity on the human breast cancer cell line (MCF-7) and fibroblasts isolated from foreskin tissue was evaluated using MTT assay. Mechanisms leading to apoptosis were also investigated at the molecular level by measuring the amount of anti and pro-apoptotic proteins and at the cellular level by studying DNA fragmentation and annexin V staining by flow cytometry. The aqueous extract of Urtica Dioica showed antioxidant effects with a correlation coefficient of r 2 =0.997. Dose-dependent and anti-proliferative effects of the extract were observed only on MCF-7 cells after 72 hrs with an IC 50 value of 2 mg/ml. This anti proliferative activity was associated with an increase of apoptosis as demonstrated by DNA fragmentation, the appearance of apoptotic cells in flow cytometry analysis and an increase of the amount of calpain 1, calpastatin, caspase 3, caspase 9, Bax and Bcl-2, all proteins involved in the apoptotic pathway. This is the first time such in vitro antiproliferative effect of aqueous extract of Urtica Dioica leaf has been described for a breast cancer cell line. Our findings warrant further research on Urtica Dioica as a potential chemotherapeutic agent for breast cancer.