The Experts below are selected from a list of 30 Experts worldwide ranked by ideXlab platform
Lawrence P. Reynolds - One of the best experts on this subject based on the ideXlab platform.
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Effects of estradiol-17β on expression of mRNA for seven angiogenic factors and their receptors in the endometrium of ovariectomized (OVX) ewes
Endocrine, 2006Co-Authors: Mary Lynn Johnson, Anna T. Grazul-bilska, Dale A. Redmer, Lawrence P. ReynoldsAbstract:We have previously established an ovariectomized (OVX) ewe model to study how steroid removal and replacement affects Uterine Blood Vessel and tissue growth. Using this model, endometrial expression of mRNA for 14 angiogenic factors (7 genes and their respective receptors) in caruncular (CAR) and intercaruncular (ICAR) endometrium were evaluated by quantitative real time RT-PCR at 0 (control), 2, 4, 8, 16, or 24 h after treating OVX ewes with an estradiol-17β (E2) implant. In CAR and ICAR, compared to 0 h, the mRNA expression of vascular endothelial growth factor ( VEGF ), VEGF receptor (R)1, soluble guanylate cyclase ( GUCY1B3 ; the R for nitric oxide [NO]), hypoxia inducible factor ( HIF ) 1α , and placental growth factor ( PIGF ) increased by 4 h after E2-treatment, but basic fibroblast growth factor ( FGF2 ), endothelial NO synthase ( NOS3 ), angiopoietin ( ANGPT ) 1, ANGPT2, ANGPT receptor Tie2 by 2 h after E2. Expression of mRNA for FGFR2IIIc was increased at 2 h by E2-treatment in ICAR, but not in CAR. By contrast, expression of neuropilin ( NP ) 1 mRNA was increased at 2 h in CAR, but not ICAR. The mRNA expression of VEGF, FGF2, HIF1α , and PIGF was positively correlated with mRNA expression of NOS3, VEGFR1 , and Tie2 suggesting some E2-stimulated interactions between these factors in promoting Blood Vessel growth. Thus, several major angiogenic factors and their receptors are increased within hours after E2-treatment, which indicates that E2 plays a role in regulation of angiogenesis in the uterus. By using the OVX ewe model, we may begin to understand the molecular basis of E2 effects on angiogenesis in the endometrium and, eventually, how angiogenesis is regulated in normal versus pathological conditions.
Philip N. Baker - One of the best experts on this subject based on the ideXlab platform.
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VEGF via VEGF receptor-1 (Flt-1) mimics preeclamptic plasma in inhibiting Uterine Blood Vessel relaxation in pregnancy: implications in the pathogenesis of preeclampsia.
Laboratory investigation; a journal of technical methods and pathology, 1999Co-Authors: Jeremy C. Brockelsby, R Hayman, Asif Ahmed, Averil Y. Warren, Ian R. Johnson, Philip N. BakerAbstract:Preeclampsia is a multisystem disorder characterized by hypertension and proteinuria. There is accumulating evidence that this is a disease of the endothelium, with an as-yet unidentified circulating factor, or factors, causing the observed alteration in vascular function. We previously reported that the function of myometrial Vessels is altered on exposure to plasma from women with preeclampsia. Vascular endothelial growth factor (VEGF) is an angiogenic growth factor that acts via two high-affinity receptors (KDR and Flt-1), and its production is increased in preeclampsia. Here we report that VEGF and its Flt-1 receptor may play a pivotal role in the altered vascular function of preeclampsia. Myometrial resistance Vessels were obtained at the time of cesarean section. Using the Mulvany wire myograph, the endothelium-dependent behavior of these Vessels was studied. Incubation of Vessels from pregnant women with VEGF resulted in a reduction of endothelium-dependent relaxation that mimicked the reduction induced by plasma from women with preeclampsia. The altered function that occurred upon exposure of Vessels to VEGF or plasma from women with preeclampsia did not occur when plasma was incubated with antibodies to VEGF before Vessel incubation. The presence of an anti-KDR receptor antibody had no effect on VEGF response. However, in the presence of an anti-Flt-1 receptor antibody, VEGF or plasma from women with preeclampsia no longer attenuated the endothelium-dependent relaxation (p < 0.05). The changes observed with VEGF and plasma from women with preeclampsia and their subsequent blockade with anti-VEGF antibody and anti-Flt-1 receptor antibody strongly suggest that VEGF acting through the Flt-1 receptor is pivotal in the pathogenesis of this disease.
Mary Lynn Johnson - One of the best experts on this subject based on the ideXlab platform.
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Effects of estradiol-17β on expression of mRNA for seven angiogenic factors and their receptors in the endometrium of ovariectomized (OVX) ewes
Endocrine, 2006Co-Authors: Mary Lynn Johnson, Anna T. Grazul-bilska, Dale A. Redmer, Lawrence P. ReynoldsAbstract:We have previously established an ovariectomized (OVX) ewe model to study how steroid removal and replacement affects Uterine Blood Vessel and tissue growth. Using this model, endometrial expression of mRNA for 14 angiogenic factors (7 genes and their respective receptors) in caruncular (CAR) and intercaruncular (ICAR) endometrium were evaluated by quantitative real time RT-PCR at 0 (control), 2, 4, 8, 16, or 24 h after treating OVX ewes with an estradiol-17β (E2) implant. In CAR and ICAR, compared to 0 h, the mRNA expression of vascular endothelial growth factor ( VEGF ), VEGF receptor (R)1, soluble guanylate cyclase ( GUCY1B3 ; the R for nitric oxide [NO]), hypoxia inducible factor ( HIF ) 1α , and placental growth factor ( PIGF ) increased by 4 h after E2-treatment, but basic fibroblast growth factor ( FGF2 ), endothelial NO synthase ( NOS3 ), angiopoietin ( ANGPT ) 1, ANGPT2, ANGPT receptor Tie2 by 2 h after E2. Expression of mRNA for FGFR2IIIc was increased at 2 h by E2-treatment in ICAR, but not in CAR. By contrast, expression of neuropilin ( NP ) 1 mRNA was increased at 2 h in CAR, but not ICAR. The mRNA expression of VEGF, FGF2, HIF1α , and PIGF was positively correlated with mRNA expression of NOS3, VEGFR1 , and Tie2 suggesting some E2-stimulated interactions between these factors in promoting Blood Vessel growth. Thus, several major angiogenic factors and their receptors are increased within hours after E2-treatment, which indicates that E2 plays a role in regulation of angiogenesis in the uterus. By using the OVX ewe model, we may begin to understand the molecular basis of E2 effects on angiogenesis in the endometrium and, eventually, how angiogenesis is regulated in normal versus pathological conditions.
Gong Zai-y - One of the best experts on this subject based on the ideXlab platform.
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THE EFFECT OF “DRAGON'S Blood CAPSULE” ON NITRIC OXIDE SYNTHASE IN CHORIONIC VILLI OF EARLY PREGNANT WOMEN
Modern Preventive Medicine, 2007Co-Authors: Gong Zai-yAbstract:[Objective]To investigate the effect of traditional Chinese drug“dragon’s Blood capsule”on nitric oxide synthase in chorionic villi of early pregnant women.[Methods]The early pregnant women were randomly divided into two groups.The experimental group was treated by“dragon’s Blood capsule”and the control group was given placebo at the process of medical abortion.The level of nitric oxide synthase(NOS) in chorionic villi was measured after the treatment in each group.[Results]The level of nitric oxide synthase in chorionic villi of the control group was significantly higher than that of the experimental group(P﹤0.05) .[Conclusions]“Dragon’s Blood capsule”can reduce the level of NOS in chorionic villi even more.Consequently it reduce the level of NO which can loosen Uterine Blood Vessel and restrain the gathering of the platelet and finally reduce bleeding.
Biswas Shivhare Sourima - One of the best experts on this subject based on the ideXlab platform.
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Investigation of Uterine Blood Vessel development in heavy menstrual bleeding
Newcastle University, 2015Co-Authors: Biswas Shivhare SourimaAbstract:PhD ThesisHeavy menstrual bleeding (HMB) affects 30% of women of reproductive age, accounting for two-thirds of all hysterectomies. 50% HMB cases are unexplained and current treatment options often compromise fertility. Understanding the mechanisms underlying HMB is therefore critical to delineate potential pathways for therapeutic intervention. Previous studies suggested structural and functional roles for endometrial Blood Vessels in the pathogenesis of HMB. Endothelial cells (ECs) are associated with growth and formation of Blood Vessels; extracellular matrix (ECM) provides the framework for maintaining vascular structure, while vascular smooth muscle cell (VSMC) differentiation regulates Blood flow and pressure. Altered endometrial vascular maturation is seen in recurrent miscarriage in association with increased Uterine natural killer (uNK) cell number and variation in endometrial angiogenic growth factor (AGF) expression, but these processes have not been extensively studied in HMB. This study aimed to assess vascular maturation and AGF expression in HMB and investigate some of their effects in vitro. A study of VSMC differentiation revealed reduced endometrial calponin expression, suggesting a dysfunctional vascular contraction mechanism in HMB. This was associated with increased osteopontin expression, supporting previous evidence of regulation of vascular calponin expression by osteopontin. Endometrial collagen IV expression was lower in HMB, reflecting weaker vascular structure and definition. An altered pattern of uNK cell density in HMB may reflect altered in situ differentiation and or proliferation, which could impact vascular development and/or endometrial preparation for menstruation. VSMCs showed decreased endometrial expression of PDGFRα and TGFβRI, highlighting altered state of VSMC differentiation and potentially dysregulated endometrial vascular development in HMB. Furthermore, preliminary results indicated that PDGFBB helped to maintain EC ‘honeycomb’ structures, while anti-PDGFBB or TGFβ1 treatment decreased their numbers in vitro. Collectively, this study has highlighted key alterations in Blood Vessels in HMB, and established an in vitro model for future functional studies of Blood Vessel development.Newcastle University and Wellbeing of Wome
