The Experts below are selected from a list of 60 Experts worldwide ranked by ideXlab platform
Felice Petraglia - One of the best experts on this subject based on the ideXlab platform.
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Recent advances in understanding and managing adenomyosis [version 1; referees: 2 approved]
F1000 Research Ltd, 2019Co-Authors: Silvia Vannuccini, Felice PetragliaAbstract:Adenomyosis is a benign Uterine Disorder in which endometrial glands and stroma are pathologically demonstrated in the Uterine myometrium and it is considered a specific entity in the PALM-COEIN FIGO (polyp; adenomyosis; leiomyoma; malignancy and hyperplasia; coagulopathy; ovulatory dysfunction; endometrial; iatrogenic; and not yet classified – International Federation of Gynecology and Obstetrics) classification of causes of abnormal Uterine bleeding (AUB). Although it has always been considered the classic condition of multiparous women over 40 years old who have pain and heavy menstrual bleeding, diagnosed at hysterectomy, the epidemiological scenario has completely changed. Adenomyosis is increasingly identified in young women with pain, AUB, infertility, or no symptoms by using imaging techniques such as transvaginal ultrasound and magnetic resonance. However, there is no agreement on the definition and classification of adenomyotic lesions from both the histopathology and the imaging point of view, and the diagnosis remains difficult and unclear. A uniform and shared reporting system needs to be implemented in order to improve our understanding on imaging features, their relationship with pathogenic theories, and their importance in terms of clinical symptoms and response to treatment. In fact, adenomyosis pathogenesis remains elusive and not a single theory can explain all of the different phenotypes of the disease. Furthermore, adenomyosis often coexists with other gynecological conditions, such as endometriosis and Uterine fibroids, increasing the heterogeneity of available data. Treatment requires a lifelong management plan as the disease has a negative impact on quality of life in terms of menstrual symptoms, fertility, and pregnancy outcome and has a high risk of miscarriage and obstetric complications
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Pathogenesis of adenomyosis: an update on molecular mechanisms.
Reproductive biomedicine online, 2017Co-Authors: Silvia Vannuccini, Claudia Tosti, Francisco Carmona, S. Joseph Huang, Charles Chapron, Sun-wei Guo, Felice PetragliaAbstract:Adenomyosis is a Uterine Disorder becoming more commonly diagnosed in women of reproductive age because of diagnostic imaging advancements. The new epidemiological scenario and the clinical evidence of pelvic pain, abnormal Uterine bleeding and infertility are changing the classic perspective of adenomyosis as a premenopausal disease. In the last decade, the evaluation of multiple molecular mediators has improved our knowledge of pathogenic mechanisms of adenomyosis, supporting that this is an independent disease from endometriosis. Although they share common genetic mutations and epigenetic changes in sex steroid hormone receptors and similar inflammatory mediators, an increasing number of recent studies have shown pathogenic pathways specific for adenomyosis. A PubMed search up to October 2016 summarizes the key mediators of pain, abnormal Uterine bleeding and infertility in adenomyosis, including sex steroid hormone receptors, inflammatory molecules, extracellular matrix enzymes, growth factors and neuroangiogenic factors.
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Expression of Inflammatory and Neurogenic Mediators in Adenomyosis: A Pathogenetic Role
Reproductive Sciences, 2017Co-Authors: Patrizia Carrarelli, Chih-feng Yen, Lucia Funghi, Felice Arcuri, Claudia Tosti, Giuseppe Bifulco, Alice Luddi, Chyi-long Lee, Felice PetragliaAbstract:Objective Adenomyosis is a Uterine Disorder characterized by dysmenorrhea, dyspareunia, abnormal Uterine bleeding, and infertility. Pathogenesis indicates that endometrial cells invade and proliferate within myometrium, and inflammatory mediators participate to the intense painful symptoms. The aim of the present study was to investigate the messenger RNA (mRNA) and protein expression of inflammatory (interleukin 1β [IL-1β], corticotropin-releasing hormone [CRH], urocortin [Ucn]) and neurogenic (nerve growth factors [NGFs], synaptophysin [SYN], microtubule-associated protein 2 [MAP2]) factors in adenomyotic nodules. Materials and Methods This prospective study enrolled 16 women, 8 women with nodular adenomyosis and 8 control women undergoing to hysterectomy. Specimens from adenomyotic nodules and eutopic endometrium were collected after surgery. Endometrial tissue was also obtained from the control group and also used for preparing primary culture of human endometrial stromal cells (HESCs). Messenger RNA expression of inflammatory mediators (IL-1β, CRH, and Ucn) and neurogenic factors (NGF, SYN, and MAP2) was analyzed by real-time polymerase chain reaction. The in vitro effects of CRH/Ucn on NGF or SYN mRNA expression were also investigated. Results Adenomyotic nodules highly expressed IL-1β, CRH, and Ucn mRNAs, as well as NGF, SYN, and MAP2 mRNAs ( P
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Expression of Inflammatory and Neurogenic Mediators in Adenomyosis: A Pathogenetic Role
Reproductive sciences (Thousand Oaks Calif.), 2016Co-Authors: Patrizia Carrarelli, Chih-feng Yen, Lucia Funghi, Felice Arcuri, Claudia Tosti, Giuseppe Bifulco, Alice Luddi, Chyi-long Lee, Felice PetragliaAbstract:Objective:Adenomyosis is a Uterine Disorder characterized by dysmenorrhea, dyspareunia, abnormal Uterine bleeding, and infertility. Pathogenesis indicates that endometrial cells invade and proliferate within myometrium, and inflammatory mediators participate to the intense painful symptoms. The aim of the present study was to investigate the messenger RNA (mRNA) and protein expression of inflammatory (interleukin 1β [IL-1β], corticotropin-releasing hormone [CRH], urocortin [Ucn]) and neurogenic (nerve growth factors [NGFs], synaptophysin [SYN], microtubule-associated protein 2 [MAP2]) factors in adenomyotic nodules.Materials and Methods:This prospective study enrolled 16 women, 8 women with nodular adenomyosis and 8 control women undergoing to hysterectomy. Specimens from adenomyotic nodules and eutopic endometrium were collected after surgery. Endometrial tissue was also obtained from the control group and also used for preparing primary culture of human endometrial stromal cells (HESCs). Messenger RNA e...
Frédéric Batteux - One of the best experts on this subject based on the ideXlab platform.
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The Disease Phenotype of Adenomyosis-Affected Women Correlates With Specific Serum Cytokine Profiles
Reproductive Sciences, 2019Co-Authors: Mathilde Bourdon, Pietro Santulli, Sandrine Chouzenoux, Chloé Maignien, Karine Bailly, Muriel Andrieu, Anne-elodie Millischer, Ludivine Doridot, Louis Marcellin, Frédéric BatteuxAbstract:Background Adenomyosis (ADE) is an enigmatic Uterine Disorder. Several types have been previously described: diffuse adenomyosis (DIF-ADE), focal adenomyosis (FOC-ADE), and association of focal and diffuse lesions (FOC/DIF-ADE). Abnormal immune phenomena have been described that may provide an understanding of the pathophysiology of adenomyosis. However, the immune imbalance in adenomyosis is however still poorly understood. Objective To compare serum cytokine profiles for the various adenomyosis phenotypes in adenomyosis versus disease-free women. Materials and Methods This cohort study included 80 women. Based on the magnetic resonance imaging (MRI) findings, the women were allocated to the ADE group (n = 60) and the control group (n = 20). The ADE group was further subdivided according to the phenotype: DIF-ADE, FOC-ADE, and FOC/DIF-ADE. For all of the women, serum cytokine levels were assayed by multiplex immunoassay. Results Serum levels of interleukin (IL) 23 (237.77 pg/mL ± 70.97 in the ADE-group versus 1855.04 ± 1411.33 in the control group, P =.019), IL25 (31.98 ± 8.54 vs 222.08 ± 170.90, respectively, P =.006), IL31 (10.13 ± 3.83 vs 91.51 ± 71.21, respectively, P =.034), IL33 (3.77 ± 1.23 vs 17.86 ± 11.49, respectively, P =.016), and IL17F (16.29 ± 2.35 vs 30.12 ± 8.29, respectively, P =.042) were significantly lower in the women with adenomyosis when compared to the controls In the FOC/DIF-ADE group, the serum levels of IL23, IL31, IL25, and IL33 were significantly lower when compared to the control group. Conclusion Serum levels of IL23, IL31, IL25, and IL33 were lower in women exhibiting adenomyosis forms with associated diffuse and focal lesions when compared with controls. The pathogenesis of adenomyosis may be associated with an immunotolerant process that is more pronounced in associated FOC/DIF-ADE.
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The Disease Phenotype of Adenomyosis-Affected Women Correlates With Specific Serum Cytokine Profiles.
Reproductive sciences (Thousand Oaks Calif.), 2018Co-Authors: Mathilde Bourdon, Pietro Santulli, Sandrine Chouzenoux, Chloé Maignien, Karine Bailly, Muriel Andrieu, Anne-elodie Millischer, Ludivine Doridot, Louis Marcellin, Frédéric BatteuxAbstract:Background:Adenomyosis (ADE) is an enigmatic Uterine Disorder. Several types have been previously described: diffuse adenomyosis (DIF-ADE), focal adenomyosis (FOC-ADE), and association of focal and...
Claudia Tosti - One of the best experts on this subject based on the ideXlab platform.
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Pathogenesis of adenomyosis: an update on molecular mechanisms.
Reproductive biomedicine online, 2017Co-Authors: Silvia Vannuccini, Claudia Tosti, Francisco Carmona, S. Joseph Huang, Charles Chapron, Sun-wei Guo, Felice PetragliaAbstract:Adenomyosis is a Uterine Disorder becoming more commonly diagnosed in women of reproductive age because of diagnostic imaging advancements. The new epidemiological scenario and the clinical evidence of pelvic pain, abnormal Uterine bleeding and infertility are changing the classic perspective of adenomyosis as a premenopausal disease. In the last decade, the evaluation of multiple molecular mediators has improved our knowledge of pathogenic mechanisms of adenomyosis, supporting that this is an independent disease from endometriosis. Although they share common genetic mutations and epigenetic changes in sex steroid hormone receptors and similar inflammatory mediators, an increasing number of recent studies have shown pathogenic pathways specific for adenomyosis. A PubMed search up to October 2016 summarizes the key mediators of pain, abnormal Uterine bleeding and infertility in adenomyosis, including sex steroid hormone receptors, inflammatory molecules, extracellular matrix enzymes, growth factors and neuroangiogenic factors.
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Expression of Inflammatory and Neurogenic Mediators in Adenomyosis: A Pathogenetic Role
Reproductive Sciences, 2017Co-Authors: Patrizia Carrarelli, Chih-feng Yen, Lucia Funghi, Felice Arcuri, Claudia Tosti, Giuseppe Bifulco, Alice Luddi, Chyi-long Lee, Felice PetragliaAbstract:Objective Adenomyosis is a Uterine Disorder characterized by dysmenorrhea, dyspareunia, abnormal Uterine bleeding, and infertility. Pathogenesis indicates that endometrial cells invade and proliferate within myometrium, and inflammatory mediators participate to the intense painful symptoms. The aim of the present study was to investigate the messenger RNA (mRNA) and protein expression of inflammatory (interleukin 1β [IL-1β], corticotropin-releasing hormone [CRH], urocortin [Ucn]) and neurogenic (nerve growth factors [NGFs], synaptophysin [SYN], microtubule-associated protein 2 [MAP2]) factors in adenomyotic nodules. Materials and Methods This prospective study enrolled 16 women, 8 women with nodular adenomyosis and 8 control women undergoing to hysterectomy. Specimens from adenomyotic nodules and eutopic endometrium were collected after surgery. Endometrial tissue was also obtained from the control group and also used for preparing primary culture of human endometrial stromal cells (HESCs). Messenger RNA expression of inflammatory mediators (IL-1β, CRH, and Ucn) and neurogenic factors (NGF, SYN, and MAP2) was analyzed by real-time polymerase chain reaction. The in vitro effects of CRH/Ucn on NGF or SYN mRNA expression were also investigated. Results Adenomyotic nodules highly expressed IL-1β, CRH, and Ucn mRNAs, as well as NGF, SYN, and MAP2 mRNAs ( P
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Expression of Inflammatory and Neurogenic Mediators in Adenomyosis: A Pathogenetic Role
Reproductive sciences (Thousand Oaks Calif.), 2016Co-Authors: Patrizia Carrarelli, Chih-feng Yen, Lucia Funghi, Felice Arcuri, Claudia Tosti, Giuseppe Bifulco, Alice Luddi, Chyi-long Lee, Felice PetragliaAbstract:Objective:Adenomyosis is a Uterine Disorder characterized by dysmenorrhea, dyspareunia, abnormal Uterine bleeding, and infertility. Pathogenesis indicates that endometrial cells invade and proliferate within myometrium, and inflammatory mediators participate to the intense painful symptoms. The aim of the present study was to investigate the messenger RNA (mRNA) and protein expression of inflammatory (interleukin 1β [IL-1β], corticotropin-releasing hormone [CRH], urocortin [Ucn]) and neurogenic (nerve growth factors [NGFs], synaptophysin [SYN], microtubule-associated protein 2 [MAP2]) factors in adenomyotic nodules.Materials and Methods:This prospective study enrolled 16 women, 8 women with nodular adenomyosis and 8 control women undergoing to hysterectomy. Specimens from adenomyotic nodules and eutopic endometrium were collected after surgery. Endometrial tissue was also obtained from the control group and also used for preparing primary culture of human endometrial stromal cells (HESCs). Messenger RNA e...
Patricia Diaz-gimeno - One of the best experts on this subject based on the ideXlab platform.
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Uterine Disorders affecting female fertility: what are the molecular functions altered in endometrium?
Fertility and sterility, 2020Co-Authors: Almudena Devesa-peiro, Patricia Sebastián-león, Francisco García-garcía, Vicente Arnau, Alejandro Alemán, Antonio Pellicer, Patricia Diaz-gimenoAbstract:Objective To determine the molecular functions of genes exhibiting altered expression in the endometrium of women with Uterine Disorders affecting fertility. Design Retrospective analysis integrating case and control data from multiple cohorts with endometrium gene expression in women with Uterine Disorders. Setting Infertility research department affiliated with a university hospital. Patient(s) Two hundred and forty women, 121 of whom were controls, 119 of whom had endometrial adenocarcinoma (ADC), recurrent implantation failure (RIF), recurrent pregnancy loss (RPL), or stage II–IV endometriosis. Intervention(s) None. Main Outcome Measure(s) Genomewide gene expression and altered molecular functions in the endometrium of each Uterine Disorder. Result(s) Using robust analysis methods, we identified statistically significantly altered endometrial functions in all the Uterine Disorders. Cell cycle alterations were shared among all the pathologies investigated. Endometriosis was characterized by the down-regulation of ciliary processes. Among the endometriosis, ADC, and RIF samples, mitochondrial dysfunction and protein degradation were shared dysregulated processes. In addition, RPL had the most distinct functional profile, and 95% of affected functions were down-regulated. Conclusion(s) The most robust functions dysregulated in the endometrium of patients with Uterine Disorders across sample cohorts implicated an endometrial factor at the gene expression level. This shared endometrial factor affects endometrial receptivity processes.
Mathilde Bourdon - One of the best experts on this subject based on the ideXlab platform.
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The Disease Phenotype of Adenomyosis-Affected Women Correlates With Specific Serum Cytokine Profiles
Reproductive Sciences, 2019Co-Authors: Mathilde Bourdon, Pietro Santulli, Sandrine Chouzenoux, Chloé Maignien, Karine Bailly, Muriel Andrieu, Anne-elodie Millischer, Ludivine Doridot, Louis Marcellin, Frédéric BatteuxAbstract:Background Adenomyosis (ADE) is an enigmatic Uterine Disorder. Several types have been previously described: diffuse adenomyosis (DIF-ADE), focal adenomyosis (FOC-ADE), and association of focal and diffuse lesions (FOC/DIF-ADE). Abnormal immune phenomena have been described that may provide an understanding of the pathophysiology of adenomyosis. However, the immune imbalance in adenomyosis is however still poorly understood. Objective To compare serum cytokine profiles for the various adenomyosis phenotypes in adenomyosis versus disease-free women. Materials and Methods This cohort study included 80 women. Based on the magnetic resonance imaging (MRI) findings, the women were allocated to the ADE group (n = 60) and the control group (n = 20). The ADE group was further subdivided according to the phenotype: DIF-ADE, FOC-ADE, and FOC/DIF-ADE. For all of the women, serum cytokine levels were assayed by multiplex immunoassay. Results Serum levels of interleukin (IL) 23 (237.77 pg/mL ± 70.97 in the ADE-group versus 1855.04 ± 1411.33 in the control group, P =.019), IL25 (31.98 ± 8.54 vs 222.08 ± 170.90, respectively, P =.006), IL31 (10.13 ± 3.83 vs 91.51 ± 71.21, respectively, P =.034), IL33 (3.77 ± 1.23 vs 17.86 ± 11.49, respectively, P =.016), and IL17F (16.29 ± 2.35 vs 30.12 ± 8.29, respectively, P =.042) were significantly lower in the women with adenomyosis when compared to the controls In the FOC/DIF-ADE group, the serum levels of IL23, IL31, IL25, and IL33 were significantly lower when compared to the control group. Conclusion Serum levels of IL23, IL31, IL25, and IL33 were lower in women exhibiting adenomyosis forms with associated diffuse and focal lesions when compared with controls. The pathogenesis of adenomyosis may be associated with an immunotolerant process that is more pronounced in associated FOC/DIF-ADE.
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The Disease Phenotype of Adenomyosis-Affected Women Correlates With Specific Serum Cytokine Profiles.
Reproductive sciences (Thousand Oaks Calif.), 2018Co-Authors: Mathilde Bourdon, Pietro Santulli, Sandrine Chouzenoux, Chloé Maignien, Karine Bailly, Muriel Andrieu, Anne-elodie Millischer, Ludivine Doridot, Louis Marcellin, Frédéric BatteuxAbstract:Background:Adenomyosis (ADE) is an enigmatic Uterine Disorder. Several types have been previously described: diffuse adenomyosis (DIF-ADE), focal adenomyosis (FOC-ADE), and association of focal and...
