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Yali Hu - One of the best experts on this subject based on the ideXlab platform.
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umbilical cord derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of mmp 9 in rat Uterine scars
Stem Cell Research & Therapy, 2017Co-Authors: Lu Xu, Lijun Ding, Jingjie Lu, Xinan Li, Tianran Song, Lei Wang, Yali HuAbstract:Severe injuries of the uterus may trigger Uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in Uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat Uterine scars following full-thickness excision of Uterine walls. In order to establish a rat model of Uterine scars, the Uterine wall of approximately 1.0 cm in length and 0.5 cm in width (one-third of the Uterine circumference) was excised from each Uterine horn. A total of 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns) to investigate the effect of different treatments on the structure and function of Uterine scars. PBS, degradable collagen fibres, UC-MSCs or UC-MSCs mixed with gelatinous degradable collagen fibres were injected into four pre-marked points surrounding each Uterine scar, respectively. At days 30 and 60 post-transplantation, a subset of rats (n = 8 Uterine Horns) from each group was euthanized and serial sections of Uterine tissues containing the operative region were prepared. Haematoxylin-eosin staining, Masson’s trichrome staining, and immunohistochemical staining for MMP-2, MMP-9, α-SMA and vWF were performed. Finally, another subset of rats (n = 16 Uterine Horns) from each group was mated with male rats at day 60 post-transplantation and euthanized 18 days after the presence of vaginal plugs to check numbers, sizes and weights of fetuses, as well as sites of implantation. The scaffold/UC-MSCs group exhibited obvious collagen degradation compared with the other three groups. At day 60 post-transplantation, the number of MMP-9-positive cells in the scaffold/UC-MSCs group (25.96 ± 3.63) was significantly higher than that in the PBS group (8.19 ± 1.61, P < 0.01), the scaffold group (7.25 ± 2.17, P < 0.01) and the UC-MSCs group (8.31 ± 2.77, P < 0.01). The pregnancy rate in the scaffold/UC-MSCs group (10/16) was also significantly higher than that in the PBS group (2/16, P < 0.017), the scaffold group (1/16, P < 0.017) and the UC-MSCs group (3/16, P < 0.017). The scaffold/UC-MSCs system facilitated collagen degradation in Uterine scars via upregulation of MMP-9, which was secreted by transplanted UC-MSCs, and promoted regeneration of the endometrium, myometrium and blood vessels in Uterine scars. Furthermore, the scaffold/UC-MSCs-treated Uterine scars showed nearly complete restoration of receptive fertility.
Lijun Ding - One of the best experts on this subject based on the ideXlab platform.
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umbilical cord derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of mmp 9 in rat Uterine scars
Stem Cell Research & Therapy, 2017Co-Authors: Lu Xu, Lijun Ding, Jingjie Lu, Xinan Li, Tianran Song, Lei Wang, Yali HuAbstract:Severe injuries of the uterus may trigger Uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in Uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat Uterine scars following full-thickness excision of Uterine walls. In order to establish a rat model of Uterine scars, the Uterine wall of approximately 1.0 cm in length and 0.5 cm in width (one-third of the Uterine circumference) was excised from each Uterine horn. A total of 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns) to investigate the effect of different treatments on the structure and function of Uterine scars. PBS, degradable collagen fibres, UC-MSCs or UC-MSCs mixed with gelatinous degradable collagen fibres were injected into four pre-marked points surrounding each Uterine scar, respectively. At days 30 and 60 post-transplantation, a subset of rats (n = 8 Uterine Horns) from each group was euthanized and serial sections of Uterine tissues containing the operative region were prepared. Haematoxylin-eosin staining, Masson’s trichrome staining, and immunohistochemical staining for MMP-2, MMP-9, α-SMA and vWF were performed. Finally, another subset of rats (n = 16 Uterine Horns) from each group was mated with male rats at day 60 post-transplantation and euthanized 18 days after the presence of vaginal plugs to check numbers, sizes and weights of fetuses, as well as sites of implantation. The scaffold/UC-MSCs group exhibited obvious collagen degradation compared with the other three groups. At day 60 post-transplantation, the number of MMP-9-positive cells in the scaffold/UC-MSCs group (25.96 ± 3.63) was significantly higher than that in the PBS group (8.19 ± 1.61, P < 0.01), the scaffold group (7.25 ± 2.17, P < 0.01) and the UC-MSCs group (8.31 ± 2.77, P < 0.01). The pregnancy rate in the scaffold/UC-MSCs group (10/16) was also significantly higher than that in the PBS group (2/16, P < 0.017), the scaffold group (1/16, P < 0.017) and the UC-MSCs group (3/16, P < 0.017). The scaffold/UC-MSCs system facilitated collagen degradation in Uterine scars via upregulation of MMP-9, which was secreted by transplanted UC-MSCs, and promoted regeneration of the endometrium, myometrium and blood vessels in Uterine scars. Furthermore, the scaffold/UC-MSCs-treated Uterine scars showed nearly complete restoration of receptive fertility.
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Additional file 1: Figure S1. of Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat Uterine scars
2017Co-Authors: Lijun Ding, Tianran Song, Lei Wang, Yun Cao, Hui Zhu, Jianwu DaiAbstract:The study design flowchart. In order to investigate the effect of different treatments on the structure and function of Uterine scars, 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns). In addition, in order to track the transplanted UC-MSCs in the scarred areas, eight scarred Uterine Horns from four rats were randomly assigned to two groups, including a UC-MSCs group (n = 4 Uterine Horns) and scaffold/UC-MSCs group (n = 4 Uterine Horns). (TIF 495 kb
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Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat Uterine scars
'Springer Science and Business Media LLC', 2017Co-Authors: Lijun Ding, Tianran Song, Lei Wang, Yun Cao, Hui Zhu, Jianwu DaiAbstract:Abstract Background Severe injuries of the uterus may trigger Uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in Uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat Uterine scars following full-thickness excision of Uterine walls. Methods In order to establish a rat model of Uterine scars, the Uterine wall of approximately 1.0 cm in length and 0.5 cm in width (one-third of the Uterine circumference) was excised from each Uterine horn. A total of 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns) to investigate the effect of different treatments on the structure and function of Uterine scars. PBS, degradable collagen fibres, UC-MSCs or UC-MSCs mixed with gelatinous degradable collagen fibres were injected into four pre-marked points surrounding each Uterine scar, respectively. At days 30 and 60 post-transplantation, a subset of rats (n = 8 Uterine Horns) from each group was euthanized and serial sections of Uterine tissues containing the operative region were prepared. Haematoxylin-eosin staining, Masson’s trichrome staining, and immunohistochemical staining for MMP-2, MMP-9, α-SMA and vWF were performed. Finally, another subset of rats (n = 16 Uterine Horns) from each group was mated with male rats at day 60 post-transplantation and euthanized 18 days after the presence of vaginal plugs to check numbers, sizes and weights of fetuses, as well as sites of implantation. Results The scaffold/UC-MSCs group exhibited obvious collagen degradation compared with the other three groups. At day 60 post-transplantation, the number of MMP-9-positive cells in the scaffold/UC-MSCs group (25.96 ± 3.63) was significantly higher than that in the PBS group (8.19 ± 1.61, P
Lu Xu - One of the best experts on this subject based on the ideXlab platform.
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umbilical cord derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of mmp 9 in rat Uterine scars
Stem Cell Research & Therapy, 2017Co-Authors: Lu Xu, Lijun Ding, Jingjie Lu, Xinan Li, Tianran Song, Lei Wang, Yali HuAbstract:Severe injuries of the uterus may trigger Uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in Uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat Uterine scars following full-thickness excision of Uterine walls. In order to establish a rat model of Uterine scars, the Uterine wall of approximately 1.0 cm in length and 0.5 cm in width (one-third of the Uterine circumference) was excised from each Uterine horn. A total of 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns) to investigate the effect of different treatments on the structure and function of Uterine scars. PBS, degradable collagen fibres, UC-MSCs or UC-MSCs mixed with gelatinous degradable collagen fibres were injected into four pre-marked points surrounding each Uterine scar, respectively. At days 30 and 60 post-transplantation, a subset of rats (n = 8 Uterine Horns) from each group was euthanized and serial sections of Uterine tissues containing the operative region were prepared. Haematoxylin-eosin staining, Masson’s trichrome staining, and immunohistochemical staining for MMP-2, MMP-9, α-SMA and vWF were performed. Finally, another subset of rats (n = 16 Uterine Horns) from each group was mated with male rats at day 60 post-transplantation and euthanized 18 days after the presence of vaginal plugs to check numbers, sizes and weights of fetuses, as well as sites of implantation. The scaffold/UC-MSCs group exhibited obvious collagen degradation compared with the other three groups. At day 60 post-transplantation, the number of MMP-9-positive cells in the scaffold/UC-MSCs group (25.96 ± 3.63) was significantly higher than that in the PBS group (8.19 ± 1.61, P < 0.01), the scaffold group (7.25 ± 2.17, P < 0.01) and the UC-MSCs group (8.31 ± 2.77, P < 0.01). The pregnancy rate in the scaffold/UC-MSCs group (10/16) was also significantly higher than that in the PBS group (2/16, P < 0.017), the scaffold group (1/16, P < 0.017) and the UC-MSCs group (3/16, P < 0.017). The scaffold/UC-MSCs system facilitated collagen degradation in Uterine scars via upregulation of MMP-9, which was secreted by transplanted UC-MSCs, and promoted regeneration of the endometrium, myometrium and blood vessels in Uterine scars. Furthermore, the scaffold/UC-MSCs-treated Uterine scars showed nearly complete restoration of receptive fertility.
Yuhan Meng - One of the best experts on this subject based on the ideXlab platform.
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bone mesenchymal stem cells improve pregnancy outcome by inducing maternal tolerance to the allogeneic fetus in abortion prone matings in mouse
Placenta, 2016Co-Authors: Xi Xia, Yuhan Meng, Xiaohui Zhu, Liying Yan, Y Zhang, Hongyan Jin, Jie QiaoAbstract:Abstract Introduction The successful pregnancy depends on maternal immune tolerance against the fetus. It has been reported that MSCs (mesenchymal stem cells) could play a regulatory role on immune cells such as CD4 + T cells, macrophages and NK cells, but their effect on recurrent miscarriage is unknown. Study design In a prospective study, the abortion-prone (CBA/J × DBA/2) H-2 d × H-2 k mice were utilized. Female CBA/J mice (8–10 weeks old) were injected with vehicle or MSCs via tail vein or Uterine Horns, and 14 days later, they were mated with DBA/2 males for the following experiments. Results Comparing with the control group, the embryo resorption rate in MSCs-horn injection group was dramatically decreased. MSCs were mainly located at the maternal-fetal interface, indicating that the reduction of resorption rate was due to MSCs' local effect. No matter which treatment was given, there was no significant difference in the levels of IL-4, IL-10, TNF-α and IFN-γ in CD4 + T cells and IL-10 and IL-12 in macrophages in spleens among each group. However, in contrast to other groups, the levels of IL-4 and IL-10 in CD4 + T cells localized at the maternal-fetal interface in MSCs-horn injection group were dramatically increased, and TNF-α and IFN-γ levels were notably decreased. While IL-10 expressed in macrophages was obviously higher than other groups and IL-12 in macrophages was significantly lower than other groups. Conclusions The findings indicate that MSCs injection through Uterine Horns could decrease embryo resorption rate.
Tianran Song - One of the best experts on this subject based on the ideXlab platform.
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umbilical cord derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of mmp 9 in rat Uterine scars
Stem Cell Research & Therapy, 2017Co-Authors: Lu Xu, Lijun Ding, Jingjie Lu, Xinan Li, Tianran Song, Lei Wang, Yali HuAbstract:Severe injuries of the uterus may trigger Uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in Uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat Uterine scars following full-thickness excision of Uterine walls. In order to establish a rat model of Uterine scars, the Uterine wall of approximately 1.0 cm in length and 0.5 cm in width (one-third of the Uterine circumference) was excised from each Uterine horn. A total of 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns) to investigate the effect of different treatments on the structure and function of Uterine scars. PBS, degradable collagen fibres, UC-MSCs or UC-MSCs mixed with gelatinous degradable collagen fibres were injected into four pre-marked points surrounding each Uterine scar, respectively. At days 30 and 60 post-transplantation, a subset of rats (n = 8 Uterine Horns) from each group was euthanized and serial sections of Uterine tissues containing the operative region were prepared. Haematoxylin-eosin staining, Masson’s trichrome staining, and immunohistochemical staining for MMP-2, MMP-9, α-SMA and vWF were performed. Finally, another subset of rats (n = 16 Uterine Horns) from each group was mated with male rats at day 60 post-transplantation and euthanized 18 days after the presence of vaginal plugs to check numbers, sizes and weights of fetuses, as well as sites of implantation. The scaffold/UC-MSCs group exhibited obvious collagen degradation compared with the other three groups. At day 60 post-transplantation, the number of MMP-9-positive cells in the scaffold/UC-MSCs group (25.96 ± 3.63) was significantly higher than that in the PBS group (8.19 ± 1.61, P < 0.01), the scaffold group (7.25 ± 2.17, P < 0.01) and the UC-MSCs group (8.31 ± 2.77, P < 0.01). The pregnancy rate in the scaffold/UC-MSCs group (10/16) was also significantly higher than that in the PBS group (2/16, P < 0.017), the scaffold group (1/16, P < 0.017) and the UC-MSCs group (3/16, P < 0.017). The scaffold/UC-MSCs system facilitated collagen degradation in Uterine scars via upregulation of MMP-9, which was secreted by transplanted UC-MSCs, and promoted regeneration of the endometrium, myometrium and blood vessels in Uterine scars. Furthermore, the scaffold/UC-MSCs-treated Uterine scars showed nearly complete restoration of receptive fertility.
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Additional file 1: Figure S1. of Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat Uterine scars
2017Co-Authors: Lijun Ding, Tianran Song, Lei Wang, Yun Cao, Hui Zhu, Jianwu DaiAbstract:The study design flowchart. In order to investigate the effect of different treatments on the structure and function of Uterine scars, 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns). In addition, in order to track the transplanted UC-MSCs in the scarred areas, eight scarred Uterine Horns from four rats were randomly assigned to two groups, including a UC-MSCs group (n = 4 Uterine Horns) and scaffold/UC-MSCs group (n = 4 Uterine Horns). (TIF 495 kb
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Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat Uterine scars
'Springer Science and Business Media LLC', 2017Co-Authors: Lijun Ding, Tianran Song, Lei Wang, Yun Cao, Hui Zhu, Jianwu DaiAbstract:Abstract Background Severe injuries of the uterus may trigger Uterine scar formation, ultimately leading to infertility or obstetrical complications. To date, few methods have adequately solved the problem of collagen deposition in Uterine scars. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have shown great promise in clinical applications. The objective of this study was to investigate the effect of a scaffold/UC-MSCs construct on collagen degradation and functional regeneration in rat Uterine scars following full-thickness excision of Uterine walls. Methods In order to establish a rat model of Uterine scars, the Uterine wall of approximately 1.0 cm in length and 0.5 cm in width (one-third of the Uterine circumference) was excised from each Uterine horn. A total of 128 scarred Uterine Horns from 64 rats were randomly assigned to four groups, including a PBS group (n = 32 Uterine Horns), scaffold group (n = 32 Uterine Horns), UC-MSCs group (n = 32 Uterine Horns) and scaffold/UC-MSCs group (n = 32 Uterine Horns) to investigate the effect of different treatments on the structure and function of Uterine scars. PBS, degradable collagen fibres, UC-MSCs or UC-MSCs mixed with gelatinous degradable collagen fibres were injected into four pre-marked points surrounding each Uterine scar, respectively. At days 30 and 60 post-transplantation, a subset of rats (n = 8 Uterine Horns) from each group was euthanized and serial sections of Uterine tissues containing the operative region were prepared. Haematoxylin-eosin staining, Masson’s trichrome staining, and immunohistochemical staining for MMP-2, MMP-9, α-SMA and vWF were performed. Finally, another subset of rats (n = 16 Uterine Horns) from each group was mated with male rats at day 60 post-transplantation and euthanized 18 days after the presence of vaginal plugs to check numbers, sizes and weights of fetuses, as well as sites of implantation. Results The scaffold/UC-MSCs group exhibited obvious collagen degradation compared with the other three groups. At day 60 post-transplantation, the number of MMP-9-positive cells in the scaffold/UC-MSCs group (25.96 ± 3.63) was significantly higher than that in the PBS group (8.19 ± 1.61, P
