The Experts below are selected from a list of 225 Experts worldwide ranked by ideXlab platform
K C Flanders - One of the best experts on this subject based on the ideXlab platform.
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human Uterine Tissue throughout the menstrual cycle expresses transforming growth factor beta 1 tgf beta 1 tgf beta 2 tgf beta 3 and tgf beta type ii receptor messenger ribonucleic acid and protein and contains 125i tgf beta 1 binding sites
Endocrinology, 1994Co-Authors: Nasser Chegini, Yong Zhao, R S Williams, K C FlandersAbstract:The use of isoform-specific transforming growth factor-beta (TGF beta) primers, 35S-labeled 40-mer oligonucleotide probes and polyclonal antibodies, reverse transcription-polymerase chain reaction, in situ hybridization, and immunohistochemical observations has revealed that human Uterine Tissue at various reproductive stages expresses TGF beta s and TGF beta type II receptor messenger RNAs (mRNAs) and proteins. The reverse transcription-polymerase chain reaction revealed the predicted 443-, 310-, 524-, and 431-basepair fragments for TGF beta 1, TGF beta 2, TGF beta 3, and TGF beta type II receptor, respectively, in both endometrial and myometrial Tissues, which were further verified by restriction enzyme analysis. In situ hybridization and immunohistochemical observations indicated that all Uterine cell types express TGF beta s mRNAs and proteins. In the functionalis region, endometrial luminal and glandular epithelial cells are the primary cell types expressing TGF beta s mRNAs and proteins, with lesser...
Nasser Chegini - One of the best experts on this subject based on the ideXlab platform.
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human Uterine Tissue throughout the menstrual cycle expresses transforming growth factor beta 1 tgf beta 1 tgf beta 2 tgf beta 3 and tgf beta type ii receptor messenger ribonucleic acid and protein and contains 125i tgf beta 1 binding sites
Endocrinology, 1994Co-Authors: Nasser Chegini, Yong Zhao, R S Williams, K C FlandersAbstract:The use of isoform-specific transforming growth factor-beta (TGF beta) primers, 35S-labeled 40-mer oligonucleotide probes and polyclonal antibodies, reverse transcription-polymerase chain reaction, in situ hybridization, and immunohistochemical observations has revealed that human Uterine Tissue at various reproductive stages expresses TGF beta s and TGF beta type II receptor messenger RNAs (mRNAs) and proteins. The reverse transcription-polymerase chain reaction revealed the predicted 443-, 310-, 524-, and 431-basepair fragments for TGF beta 1, TGF beta 2, TGF beta 3, and TGF beta type II receptor, respectively, in both endometrial and myometrial Tissues, which were further verified by restriction enzyme analysis. In situ hybridization and immunohistochemical observations indicated that all Uterine cell types express TGF beta s mRNAs and proteins. In the functionalis region, endometrial luminal and glandular epithelial cells are the primary cell types expressing TGF beta s mRNAs and proteins, with lesser...
M.a.f. Gimeno - One of the best experts on this subject based on the ideXlab platform.
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Regulation by nitric oxide of prostaglandin E synthesis and spontaneous motility in rat Uterine Tissue.
Prostaglandins leukotrienes and essential fatty acids, 1998Co-Authors: A.b. Motta, M. Chaud, M.a.f. GimenoAbstract:Abstract We explored the role of endogenous nitric oxide (NO) in the spontaneous motility of Uterine Tissue from pseudopregnant (psp) rats and the correlation between this action and the uterotonic prostaglandin (PG) E production. We worked in the early psp (on day 5 of psp), and in late psp (on day 8 and day 9). Treatment with N G -monomethyl- l -arginine l -NMMA (300 μM), a competitive nitric oxide synthase (NOS) inhibitor, did not modify isometric developed tension (IDT) and frequency of contractions (FC) on day 5 of psp; on day 8, Tissue pretreated with l -NMMA showed an increase in the IDT and FC compared with controls, while on day 9 of psp, both IDT and FC showed a lower stability after treatment with the inhibitor. These data suggest that NO modulates Uterine motility on day 8 (decreasing it) and on day 9 of psp (enhancing it). We also evaluated the total NOS activity and that of its isoforms at the three times mentioned, demonstrating that total NOS activity was higher on day 5 of psp and decreased with psp development. On day 5 of psp, calcium-dependent and calcium-independent NOS each forms around 50% of total NOS activity. On day 8 of psp, the calcium-dependent was the predominant NOS form, while on day 9 of psp, the Uterine Tissue showed a higher calcium-independent form of the enzyme. In view of the fact that we found an inhibitor effect of the endogenous NO in Uterine contractility on day 8 of psp and an inverse action on day 9 of psp (enhancing Uterine contractility), we suggest that the NOS calcium-dependent form could be responsible for Uterine contractility in psp rats. Finally, we evaluated the relationship between endogenous NO and PGE production. We observed that on days 5 and 8 of psp, the L-NMMA (300 μM) treatment did not affect PGE production, but on day 9 of psp, the preincubation with the NOS inhibitor diminished PGE synthesis, suggesting that at this time endogenous NO can upregulate Uterine PGE production. These results confirm that NO can modulate Uterine motility by means of PGE production. In summary, we suggest that in Uterine Tissue from psp rats, the NO system can alternatively decrease or increase Uterine contractions, this last effect by enhancing Uterine PGE synthesis.
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Eicosanoid production and phospholipase A2 activity in Uterine Tissue from castrated rats with non-insulin dependent diabetes mellitus.
Prostaglandins leukotrienes and essential fatty acids, 1995Co-Authors: Alicia Jawerbaum, J.r. Catafau, Elida González, Ana Maria Franchi, Emilio Gelpí, V. Novaro, G. Gomez, M.a.f. GimenoAbstract:In Uterine Tissue obtained from castrated control and non-insulin dependent diabetic (NIDDM) rats, eicosanoid production and its regulation by glucose levels and by the activity of phospholipase A2 (PLA2) was assessed. Basal outputs of prostaglandins (PGs) PGE2, PGE1, PGF2 alpha, 6-keto-PGF1 alpha (indicating the production of prostacyclin), thromboxane B2 (TXB2) (indicating the generation of TXA2) and leukotriene B4 (LTB4) were similar in control and NIDDM Uterine preparations as assessed by RIA. When Uterine conversion of labelled arachidonate into different prostanoids was evaluated, generation of 6-keto-PGF1 alpha, PGE2 and PGF2 alpha was similar in control and NIDDM Uterine Tissue, while TXB2 production was higher in the diabetic group. Moreover, when control Tissue was incubated in the presence of elevated concentrations of glucose (22 mM) and compared to control Tissue incubated in concentrations of glucose 11 mM, similar generation of 6-keto-PGF1 alpha, PGE2 and PGF2 alpha was observed, and higher concentrations of TXB2 were found, similar to those observed in diabetic Uterine Tissue. When NIDDM Uterine Tissue was incubated in the presence of glucose 22 mM, no difference in any prostanoid evaluated was observed when compared to values obtained in the presence of glucose 11 mM. In this work we have observed in NIDDM Uterine Tissue a normal TXA2 production when evaluated by RIA from endogenous arachidonic acid (AA) and a higher TXA2 generation from exogenous labelled AA. In addition PLA2 activity was found diminished in the NIDDM uteri in comparison to control uteri. A role of the diminished PLA2 as a protective mechanism that avoids TXA2 overproduction in Uterine Tissue from NIDDM rats is discussed.
Beat M. Jucker - One of the best experts on this subject based on the ideXlab platform.
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Noninvasive assessment of ectopic Uterine Tissue development in rats using magnetic resonance imaging.
Fertility and Sterility, 2007Co-Authors: Stephen C. Lenhard, Robin E. Haimbach, Anthony C. Sulpizio, David P. Brooks, Jeffrey D. Bray, Beat M. JuckerAbstract:Objective To non-invasively characterize ectopic Uterine Tissue (EUT) development in a modified autologous rat surgical model of endometriosis using magnetic resonance imaging (MRI). Design Investigational MRI study. Setting A pharmaceutical company. Animal(s) Female Sprague Dawley rats. Intervention(s) Uterine Tissue was autotransplanted on the right peritoneal wall of rats. Rats were serially imaged after surgery and after endogenous hormone suppression, hormone supplementation, or ovariectomy. In addition, an MRI contrast agent was administered to examine EUT perfusion characteristics. Main Outcome Measure(s) Changes in transplanted EUT volume and perfusion were monitored using MRI. Result(s) The EUT growth could be readily monitored non-invasively by MRI. Although EUT growth was rapid during the initial 4 days after surgery, volume stabilized by the third week and maintained for at least 9 weeks after transplantation. The EUT volumes varied with the estrous cycle and were hormonally sensitive to ovariectomy, to Antide (GnRH antagonist), and to Antide followed by 17β-E 2 supplementation. The use of an MRI contrast agent facilitated visualization of EUT wall perfusion. Conclusion(s) MRI allows for noninvasive, dynamic evaluation of transplanted EUT growth in the rat. This reproducible model will allow for performing quantifiable pharmacologic studies in pre-clinical drug discovery for therapies targeting endometriosis.
Yong Zhao - One of the best experts on this subject based on the ideXlab platform.
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human Uterine Tissue throughout the menstrual cycle expresses transforming growth factor beta 1 tgf beta 1 tgf beta 2 tgf beta 3 and tgf beta type ii receptor messenger ribonucleic acid and protein and contains 125i tgf beta 1 binding sites
Endocrinology, 1994Co-Authors: Nasser Chegini, Yong Zhao, R S Williams, K C FlandersAbstract:The use of isoform-specific transforming growth factor-beta (TGF beta) primers, 35S-labeled 40-mer oligonucleotide probes and polyclonal antibodies, reverse transcription-polymerase chain reaction, in situ hybridization, and immunohistochemical observations has revealed that human Uterine Tissue at various reproductive stages expresses TGF beta s and TGF beta type II receptor messenger RNAs (mRNAs) and proteins. The reverse transcription-polymerase chain reaction revealed the predicted 443-, 310-, 524-, and 431-basepair fragments for TGF beta 1, TGF beta 2, TGF beta 3, and TGF beta type II receptor, respectively, in both endometrial and myometrial Tissues, which were further verified by restriction enzyme analysis. In situ hybridization and immunohistochemical observations indicated that all Uterine cell types express TGF beta s mRNAs and proteins. In the functionalis region, endometrial luminal and glandular epithelial cells are the primary cell types expressing TGF beta s mRNAs and proteins, with lesser...
