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Hideyuki Yamamoto - One of the best experts on this subject based on the ideXlab platform.
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spinal glycinergic and gamma aminobutyric acid ergic neurons inhibit the micturition reflex after electrical stimulation of the perineum in rats with pelvic venous congestion
International Journal of Urology, 2019Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Tomoyuki Ueda, Katsuhiko Noguchi, Hideyuki YamamotoAbstract:Objectives To examine whether electrical stimulation of the perineum inhibited urinary frequency in rats with pelvic venous congestion, and whether electrical stimulation influences spinal glycinergic/gamma-aminobutyric acid-ergic neurons. Methods Bilateral common iliac Veins and bilateral Uterine Veins were ligated to create pelvic venous congestion rats. At 4 weeks after ligation, cystometry was carried out before and after electrical stimulation with/without intrathecal injection of strychnine (a glycine receptor antagonist) and/or bicuculline (a gamma-aminobutyric acid type A receptor antagonist). In addition, measurement of amino acid levels in the lumbosacral cord was carried out with/without electrical stimulation, and cystometry was carried out after oral administration of glycine. Results Continuous cystometry showed that the interval between bladder contractions was shorter in pelvic venous congestion rats than in sham rats. Electrical stimulation did not change cystometric parameters in sham rats, but the interval between bladder contractions was increased by electrical stimulation in pelvic venous congestion rats. Electrical stimulation increased the levels of glutamic acid, glycine, gamma-aminobutyric acid, and taurine in the lumbosacral cord of pelvic venous congestion rats. Intrathecal strychnine abolished the effects of electrical stimulation in pelvic venous congestion rats, and intrathecal administration of both strychnine and bicuculline shortened the interval between bladder contractions more than before electrical stimulation. Oral administration of glycine (3%) to pelvic venous congestion rats increased bladder capacity. Conclusions Electrical stimulation of the perineum inhibits urinary frequency mainly through activation of spinal glycinergic neurons, and partly through activation of gamma-aminobutyric acid-ergic neurons in a rat model of pelvic venous congestion.
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spinal glycinergic and gamma aminobutyric acid ergic neurons inhibit the micturition reflex after electrical stimulation of the perineum in rats with pelvic venous congestion
International Journal of Urology, 2019Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Tomoyuki Ueda, Katsuhiko Noguchi, Hideyuki YamamotoAbstract:OBJECTIVES To examine whether electrical stimulation of the perineum inhibited urinary frequency in rats with pelvic venous congestion, and whether electrical stimulation influences spinal glycinergic/gamma-aminobutyric acid-ergic neurons. METHODS Bilateral common iliac Veins and bilateral Uterine Veins were ligated to create pelvic venous congestion rats. At 4 weeks after ligation, cystometry was carried out before and after electrical stimulation with/without intrathecal injection of strychnine (a glycine receptor antagonist) and/or bicuculline (a gamma-aminobutyric acid type A receptor antagonist). In addition, measurement of amino acid levels in the lumbosacral cord was carried out with/without electrical stimulation, and cystometry was carried out after oral administration of glycine. RESULTS Continuous cystometry showed that the interval between bladder contractions was shorter in pelvic venous congestion rats than in sham rats. Electrical stimulation did not change cystometric parameters in sham rats, but the interval between bladder contractions was increased by electrical stimulation in pelvic venous congestion rats. Electrical stimulation increased the levels of glutamic acid, glycine, gamma-aminobutyric acid, and taurine in the lumbosacral cord of pelvic venous congestion rats. Intrathecal strychnine abolished the effects of electrical stimulation in pelvic venous congestion rats, and intrathecal administration of both strychnine and bicuculline shortened the interval between bladder contractions more than before electrical stimulation. Oral administration of glycine (3%) to pelvic venous congestion rats increased bladder capacity. CONCLUSIONS Electrical stimulation of the perineum inhibits urinary frequency mainly through activation of spinal glycinergic neurons, and partly through activation of gamma-aminobutyric acid-ergic neurons in a rat model of pelvic venous congestion.
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Spinal glycinergic and gamma-aminobutyric acid-ergic neurons inhibit the micturition reflex after electrical stimulation of the perineum in rats with pelvic venous congestion.
International journal of urology : official journal of the Japanese Urological Association, 2019Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Tomoyuki Ueda, Katsuhiko Noguchi, Hideyuki YamamotoAbstract:To examine whether electrical stimulation of the perineum inhibited urinary frequency in rats with pelvic venous congestion, and whether electrical stimulation influences spinal glycinergic/gamma-aminobutyric acid-ergic neurons. Bilateral common iliac Veins and bilateral Uterine Veins were ligated to create pelvic venous congestion rats. At 4 weeks after ligation, cystometry was carried out before and after electrical stimulation with/without intrathecal injection of strychnine (a glycine receptor antagonist) and/or bicuculline (a gamma-aminobutyric acid type A receptor antagonist). In addition, measurement of amino acid levels in the lumbosacral cord was carried out with/without electrical stimulation, and cystometry was carried out after oral administration of glycine. Continuous cystometry showed that the interval between bladder contractions was shorter in pelvic venous congestion rats than in sham rats. Electrical stimulation did not change cystometric parameters in sham rats, but the interval between bladder contractions was increased by electrical stimulation in pelvic venous congestion rats. Electrical stimulation increased the levels of glutamic acid, glycine, gamma-aminobutyric acid, and taurine in the lumbosacral cord of pelvic venous congestion rats. Intrathecal strychnine abolished the effects of electrical stimulation in pelvic venous congestion rats, and intrathecal administration of both strychnine and bicuculline shortened the interval between bladder contractions more than before electrical stimulation. Oral administration of glycine (3%) to pelvic venous congestion rats increased bladder capacity. Electrical stimulation of the perineum inhibits urinary frequency mainly through activation of spinal glycinergic neurons, and partly through activation of gamma-aminobutyric acid-ergic neurons in a rat model of pelvic venous congestion. © 2019 The Japanese Urological Association.
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Tadalafil improves bladder dysfunction and object recognition in rats with pelvic venous congestion.
International journal of urology : official journal of the Japanese Urological Association, 2019Co-Authors: Saori Nishijima, Kimio Sugaya, Katsumi Kadekawa, Katsuhiro Ashitomi, Tomoyuki Ueda, Hideyuki YamamotoAbstract:OBJECTIVES To examine the effects of tadalafil on bladder function and object recognition ability in rats with alterations in urinary frequency and locomotor activity as a result of pelvic venous congestion. METHODS A total of 48 female rats were divided into three groups (sham, pelvic venous congestion and pelvic venous congestion/tadalafil groups). In the pelvic venous congestion and pelvic venous congestion/tadalafil groups, the bilateral common iliac Veins and Uterine Veins were ligated under anesthesia. Rats in the pelvic venous congestion/tadalafil group received a diet containing tadalafil, and the other rats were fed a normal diet. After 4 weeks, rats underwent analysis of voiding behavior, locomotor activity, a novel object recognition test, continuous cystometry, measurement of plasma monoamines, and measurement of plasma and urinary nitric oxide metabolites. Expression of nitric oxide synthase messenger ribonucleic acid in the bladder wall was also assessed, along with histological examination of the bladder. RESULTS Rats with pelvic venous congestion showed a higher urinary frequency, lower locomotor activity, and lower plasma and urinary nitric oxide levels than sham rats. The bladder wall endothelial nitric oxide synthase messenger ribonucleic acid level was low and object recognition was impaired. Pelvic venous congestion/tadalafil rats showed improvement in locomotor activity, bladder function and object recognition compared with pelvic venous congestion rats, as well as elevation of plasma and urinary nitric oxide, plasma monoamines, and bladder neuronal nitric oxide synthase messenger ribonucleic acid expression. Bladder wall vascularity was greater in pelvic venous congestion/tadalafil rats compared with sham rats. CONCLUSIONS In rats with pelvic venous congestion, tadalafil might improve bladder function and the general condition by increasing blood flow to the bladder and brain, and by increasing dopamine levels.
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pelvic venous congestion induces lower urinary tract dysfunction in rats
Biomedical Research-tokyo, 2018Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Katsuhiro Ashitomi, Tomoyuki Ueda, Katsuhiko Noguchi, Hideyuki YamamotoAbstract:Pelvic venous congestion (PC) is thought to be related to several diseases of the lower urinary tract (LUT). We examined the characteristics of the LUT in rats with PC. To create PC, female rats were anesthetized with isoflurane, and the bilateral common iliac Veins and bilateral Uterine Veins were ligated. At 1-8 weeks after either ligation or sham surgery, we performed cystometry with or without administration of carbazochrome sodium sulfonate hydrate or propiverine hydrochloride, histologic examination of the bladder, blood flow imaging, assessment of locomotor activity, measurement of urinary 8-hydroxydeoxyguanosine (8-OHdG) and nitric oxide metabolites (NOx), and the Evans blue dye extravasation test. PC elevated frequency of urination after 2-6 weeks, and caused a decrease of spontaneous locomotor activity. In addition, there was a decrease of bladder blood flow, an increase of bladder vascular permeability, an increase of urinary 8-OHdG, a decrease of urinary NOx, and mild inflammatory changes of the bladder. In rats with PC, frequency of urination was normalized by administration of propiverine or carbazochrome. Rats with PC may be used as a model of PC associated with high frequency of urination, and this model may be useful when developing treatment for LUT symptoms associated with PC.
J. Joseph Walshe - One of the best experts on this subject based on the ideXlab platform.
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Circulating vascular cell adhesion molecule–1 in pre-eclampsia, gestational hypertension, and normal pregnancy: Evidence of selective dysregulation of vascular cell adhesion molecule–1 homeostasis in pre-eclampsia
American Journal of Obstetrics and Gynecology, 1998Co-Authors: John R. Higgins, Aikaterina Papayianni, Hugh R. Brady, Michael R. N. Darling, J. Joseph WalsheAbstract:Abstract OBJECTIVE: Our purpose was to investigate circulating levels of vascular cell adhesion molecule–1 in the peripheral and uteroplacental circulations during normotensive and hypertensive pregnancies. STUDY DESIGN: This prospective observational study involved 2 patient groups. Group 1 consisted of 22 women with pre-eclampsia and 30 normotensive women followed up longitudinally through pregnancy and post partum. There were an additional 13 women with established gestational hypertension. Group 2 consisted of 20 women with established pre-eclampsia and 19 normotensive control subjects undergoing cesarean delivery. Plasma levels of vascular cell adhesion molecule–1 were measured in blood drawn from the antecubital vein (group 1) and from both the antecubital and Uterine Veins (group 2). Data were analyzed by analysis of variance. RESULTS: In group 1 vascular cell adhesion molecule–1 levels did not change significantly throughout normal pregnancy and post partum. Women with established pre-eclampsia had increased vascular cell adhesion molecule–1 levels compared with the normotensive pregnancy group ( P = .01). Vascular cell adhesion molecule–1 levels were not elevated in women with established gestational hypertension. In group 2 significantly higher levels of vascular cell adhesion molecule–1 were detected in the uteroplacental ( P P P = .06). In contrast to vascular cell adhesion molecule–1, circulating levels of E-selectin and intercellular adhesion molecule–1, other major leukocyte adhesion molecules expressed by the endothelium, were not different in pre-eclamptic and normotensive pregnancies. CONCLUSION: Established pre-eclampsia is characterized by selective dysregulation of vascular cell adhesion molecule–1 homeostasis. This event is not an early preclinical feature of pre-eclampsia, does not persist post partum, is not a feature of nonproteinuric gestational hypertension, and is not observed with other major leukocyte adhesion molecules. Induction of vascular cell adhesion molecule–1 expression in pre-eclampsia may contribute to leukocyte-mediated tissue injury in this condition or may reflect perturbation of other, previously unrecognized, functions of this molecule in pregnancy. (Am J Obstet Gynecol 1998;179:464-9.)
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Hemostasis in the uteroplacental and peripheral circulations in normotensive and pre-eclamptic pregnancies☆☆☆★★★♢
American journal of obstetrics and gynecology, 1998Co-Authors: John R. Higgins, Michael R. N. Darling, J. Joseph Walshe, Lucy A. Norris, John BonnarAbstract:Abstract OBJECTIVE: Our purpose was to determine the hemostatic changes in the uteroplacental and peripheral circulations in normotensive and pre-eclamptic pregnancies. STUDY DESIGN: This prospective, observational study involved 2 patient groups. Group 1 consisted of 30 normotensive women and 22 women with pre-eclampsia who were followed up longitudinally through pregnancy and post partum. Group 2 consisted of 20 women with established pre-eclampsia and 19 normotensive control subjects, all undergoing cesarean section. Plasma levels of thrombin–antithrombin III complex, soluble fibrin, plasmin–α 2 -antiplasmin complex, and fibrin-degradation product (D-dimer) were measured in blood drawn from the antecubital vein (group 1) and from both the antecubital and Uterine Veins (group 2). Data were analyzed by analysis of variance. RESULTS: In group 1 levels of thrombin–antithrombin III complex, soluble fibrin, and fibrin-degradation product were significantly higher during normal pregnancy than at 6 weeks post partum. Plasmin–α 2 -antiplasmin complex levels did not change. No differences between the pre-eclamptic and normotensive pregnancy groups were found for any of the hemostatic markers. In group 2 normotensive women undergoing cesarean section, thrombin–antithrombin III complex and soluble fibrin levels were significantly higher in the Uterine vein than in the antecubital vein. In group 2 women with pre-eclampsia, thrombin–antithrombin III complex and fibrin-degradation product levels were significantly higher in the Uterine vein than in the antecubital vein. In addition, plasmin–α 2 -antiplasmin complex and fibrin-degradation product levels were higher and soluble fibrin levels were lower in the Uterine vein in the pre-eclamptic group than in the normotensive group. CONCLUSION: Both the coagulation and fibrinolytic systems are activated during normal pregnancy. Activation of these systems is more marked in the uteroplacental circulation than in the systemic circulation in both normotensive and pre-eclamptic pregnancies. An abnormal pattern of hemostasis occurs in the uteroplacental circulation in pre-eclampsia. (Am J Obstet Gynecol 1998;179:520-6.)
Kimio Sugaya - One of the best experts on this subject based on the ideXlab platform.
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effects of combined treatment with fesoterodine and mirabegron in a pelvic congestion rat model results from in vitro and in vivo functional studies
Luts: Lower Urinary Tract Symptoms, 2020Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Hidetomi Yamagami, Masanori Hizue, Yoshihiko Ito, Shizuo YamadaAbstract:OBJECTIVES To examine the effect of combining a nonselective muscarinic receptor antagonist, 5-hydroxymethyl tolterodine (an active metabolite of fesoterodine), with a β3 adrenoceptor agonist, mirabegron, in a rat model of pelvic congestion. METHODS The rat pelvic congestion model used female Sprague-Dawley rats with their bilateral common iliac and Uterine Veins ligated. Expressions of M2 and M3 receptor subtypes in the urothelium and detrusor were detected by real-time polymerase chain reaction assays. The effects of both drugs were investigated on isolated bladder strips contracted by electrical field stimulation. in vivo single cystometry was used to assess the effects of 5-hydroxymethyl tolterodine and mirabegron independently or in combination on bladder capacity, micturition pressure, and threshold pressure. RESULTS Pelvic congestion rats showed decreased bladder capacity compared with controls, but micturition pressure and threshold pressure were unchanged. Pelvic congestion model rats also demonstrated an approximately two-fold increase in expression of both M2 and M3 receptor subtypes in the urothelium. Additive relaxant effects of 5-hydroxymethyl tolterodine and mirabegron were observed in vitro in the electrical field stimulation-induced contractions of bladder strips from pelvic congestion rats. In vivo, bladder capacity was increased significantly by a combination of 5-hydroxymethyl tolterodine and mirabegron, with the combined effect exceeding the sum of the effects of monotherapies. Micturition pressure and threshold pressure did not significantly differ between groups. CONCLUSIONS The combination of 5-hydroxymethyl tolterodine with mirabegron suggests the potential of synergistic effects in a rat pelvic congestion model.
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spinal glycinergic and gamma aminobutyric acid ergic neurons inhibit the micturition reflex after electrical stimulation of the perineum in rats with pelvic venous congestion
International Journal of Urology, 2019Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Tomoyuki Ueda, Katsuhiko Noguchi, Hideyuki YamamotoAbstract:Objectives To examine whether electrical stimulation of the perineum inhibited urinary frequency in rats with pelvic venous congestion, and whether electrical stimulation influences spinal glycinergic/gamma-aminobutyric acid-ergic neurons. Methods Bilateral common iliac Veins and bilateral Uterine Veins were ligated to create pelvic venous congestion rats. At 4 weeks after ligation, cystometry was carried out before and after electrical stimulation with/without intrathecal injection of strychnine (a glycine receptor antagonist) and/or bicuculline (a gamma-aminobutyric acid type A receptor antagonist). In addition, measurement of amino acid levels in the lumbosacral cord was carried out with/without electrical stimulation, and cystometry was carried out after oral administration of glycine. Results Continuous cystometry showed that the interval between bladder contractions was shorter in pelvic venous congestion rats than in sham rats. Electrical stimulation did not change cystometric parameters in sham rats, but the interval between bladder contractions was increased by electrical stimulation in pelvic venous congestion rats. Electrical stimulation increased the levels of glutamic acid, glycine, gamma-aminobutyric acid, and taurine in the lumbosacral cord of pelvic venous congestion rats. Intrathecal strychnine abolished the effects of electrical stimulation in pelvic venous congestion rats, and intrathecal administration of both strychnine and bicuculline shortened the interval between bladder contractions more than before electrical stimulation. Oral administration of glycine (3%) to pelvic venous congestion rats increased bladder capacity. Conclusions Electrical stimulation of the perineum inhibits urinary frequency mainly through activation of spinal glycinergic neurons, and partly through activation of gamma-aminobutyric acid-ergic neurons in a rat model of pelvic venous congestion.
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spinal glycinergic and gamma aminobutyric acid ergic neurons inhibit the micturition reflex after electrical stimulation of the perineum in rats with pelvic venous congestion
International Journal of Urology, 2019Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Tomoyuki Ueda, Katsuhiko Noguchi, Hideyuki YamamotoAbstract:OBJECTIVES To examine whether electrical stimulation of the perineum inhibited urinary frequency in rats with pelvic venous congestion, and whether electrical stimulation influences spinal glycinergic/gamma-aminobutyric acid-ergic neurons. METHODS Bilateral common iliac Veins and bilateral Uterine Veins were ligated to create pelvic venous congestion rats. At 4 weeks after ligation, cystometry was carried out before and after electrical stimulation with/without intrathecal injection of strychnine (a glycine receptor antagonist) and/or bicuculline (a gamma-aminobutyric acid type A receptor antagonist). In addition, measurement of amino acid levels in the lumbosacral cord was carried out with/without electrical stimulation, and cystometry was carried out after oral administration of glycine. RESULTS Continuous cystometry showed that the interval between bladder contractions was shorter in pelvic venous congestion rats than in sham rats. Electrical stimulation did not change cystometric parameters in sham rats, but the interval between bladder contractions was increased by electrical stimulation in pelvic venous congestion rats. Electrical stimulation increased the levels of glutamic acid, glycine, gamma-aminobutyric acid, and taurine in the lumbosacral cord of pelvic venous congestion rats. Intrathecal strychnine abolished the effects of electrical stimulation in pelvic venous congestion rats, and intrathecal administration of both strychnine and bicuculline shortened the interval between bladder contractions more than before electrical stimulation. Oral administration of glycine (3%) to pelvic venous congestion rats increased bladder capacity. CONCLUSIONS Electrical stimulation of the perineum inhibits urinary frequency mainly through activation of spinal glycinergic neurons, and partly through activation of gamma-aminobutyric acid-ergic neurons in a rat model of pelvic venous congestion.
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Spinal glycinergic and gamma-aminobutyric acid-ergic neurons inhibit the micturition reflex after electrical stimulation of the perineum in rats with pelvic venous congestion.
International journal of urology : official journal of the Japanese Urological Association, 2019Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Tomoyuki Ueda, Katsuhiko Noguchi, Hideyuki YamamotoAbstract:To examine whether electrical stimulation of the perineum inhibited urinary frequency in rats with pelvic venous congestion, and whether electrical stimulation influences spinal glycinergic/gamma-aminobutyric acid-ergic neurons. Bilateral common iliac Veins and bilateral Uterine Veins were ligated to create pelvic venous congestion rats. At 4 weeks after ligation, cystometry was carried out before and after electrical stimulation with/without intrathecal injection of strychnine (a glycine receptor antagonist) and/or bicuculline (a gamma-aminobutyric acid type A receptor antagonist). In addition, measurement of amino acid levels in the lumbosacral cord was carried out with/without electrical stimulation, and cystometry was carried out after oral administration of glycine. Continuous cystometry showed that the interval between bladder contractions was shorter in pelvic venous congestion rats than in sham rats. Electrical stimulation did not change cystometric parameters in sham rats, but the interval between bladder contractions was increased by electrical stimulation in pelvic venous congestion rats. Electrical stimulation increased the levels of glutamic acid, glycine, gamma-aminobutyric acid, and taurine in the lumbosacral cord of pelvic venous congestion rats. Intrathecal strychnine abolished the effects of electrical stimulation in pelvic venous congestion rats, and intrathecal administration of both strychnine and bicuculline shortened the interval between bladder contractions more than before electrical stimulation. Oral administration of glycine (3%) to pelvic venous congestion rats increased bladder capacity. Electrical stimulation of the perineum inhibits urinary frequency mainly through activation of spinal glycinergic neurons, and partly through activation of gamma-aminobutyric acid-ergic neurons in a rat model of pelvic venous congestion. © 2019 The Japanese Urological Association.
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Tadalafil improves bladder dysfunction and object recognition in rats with pelvic venous congestion.
International journal of urology : official journal of the Japanese Urological Association, 2019Co-Authors: Saori Nishijima, Kimio Sugaya, Katsumi Kadekawa, Katsuhiro Ashitomi, Tomoyuki Ueda, Hideyuki YamamotoAbstract:OBJECTIVES To examine the effects of tadalafil on bladder function and object recognition ability in rats with alterations in urinary frequency and locomotor activity as a result of pelvic venous congestion. METHODS A total of 48 female rats were divided into three groups (sham, pelvic venous congestion and pelvic venous congestion/tadalafil groups). In the pelvic venous congestion and pelvic venous congestion/tadalafil groups, the bilateral common iliac Veins and Uterine Veins were ligated under anesthesia. Rats in the pelvic venous congestion/tadalafil group received a diet containing tadalafil, and the other rats were fed a normal diet. After 4 weeks, rats underwent analysis of voiding behavior, locomotor activity, a novel object recognition test, continuous cystometry, measurement of plasma monoamines, and measurement of plasma and urinary nitric oxide metabolites. Expression of nitric oxide synthase messenger ribonucleic acid in the bladder wall was also assessed, along with histological examination of the bladder. RESULTS Rats with pelvic venous congestion showed a higher urinary frequency, lower locomotor activity, and lower plasma and urinary nitric oxide levels than sham rats. The bladder wall endothelial nitric oxide synthase messenger ribonucleic acid level was low and object recognition was impaired. Pelvic venous congestion/tadalafil rats showed improvement in locomotor activity, bladder function and object recognition compared with pelvic venous congestion rats, as well as elevation of plasma and urinary nitric oxide, plasma monoamines, and bladder neuronal nitric oxide synthase messenger ribonucleic acid expression. Bladder wall vascularity was greater in pelvic venous congestion/tadalafil rats compared with sham rats. CONCLUSIONS In rats with pelvic venous congestion, tadalafil might improve bladder function and the general condition by increasing blood flow to the bladder and brain, and by increasing dopamine levels.
Jost Brökelmann - One of the best experts on this subject based on the ideXlab platform.
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Quantitative evaluation of the utero-ovarian venous pattern in the adult human female cadaver with plastination
World Journal of Surgery, 2004Co-Authors: Mircea L. Pavkov, Jürgen Koebke, Hans -p. Notermans, Jost BrökelmannAbstract:Hemos descrito la anatomía del sistema venoso útero-anexial en el cadaver de la mujer adulta postmenopáusica utilizando moldes de resina epoxy y la técnica de plastinaciön según von Hagen’s. Con objeto de apreciar desde un punto de vista cualitativo y cuantitativo la vena uterina, la arcada útero-anexial, la vena uterina y las ramas comunicantes entre el lado izquierdo y derecho del parámetro, se inyectó résina expoy en la vena y la arteria iliaca interna y en la vena y la arteria ovárica en seis cadáveres. En dos casos se retiraron los órganos génitales antes de la inyección, y en cuatro se efectuó una resección después de la inyección y la polimerización de la resina. Se realizó un examen macroscópico de los especimenes y se midieron digitalmente los diámetros venosos. Los diámetros medios obtenidos fueron: La vena uterina: 3.38 mm, la arcada útero-ovárica: 3.71 mm, la vena ovárica: 3.49 mm y las ramas comunicantes: 3.71. En los cuatro cadáveres que se inyectaron directamente, se llenaron las venas y se examinaron de forma más detallada. Se ha podido observar que la arcada útero-ovárica es una vena importante que tiene un diámetro similar al de las venas ovárica y uterina. Existe una importante red de anastomosis venosas entre el parámetra izquierdo y derecho y las trompas se drenan por tres venas: tubular interna, media y externa. Se observó congestión venosa pélvica en dos casos: uno en la región de la vena uterina izquierda y otro en la región de la vena ovárica izquierda. Dado que los diámetros de las venas uterina, ovárica y arcada útero-ovárica son similares, pensamos que no existe una dirección preferencial de drenaje venoso y que las venas de los órganos genitales internos se comportan como un estanque venoso. La congestión puede afectar a zonas limitadas que podrían tratarse localmente. Nous avons décrit l’anatomie du système veineux utéro-annexiel chez le cadavre de la femme adulte post ménopause en utilisant des moules de résine époxy et la technique de plastination d’après von Hagen’s. Afin d’apprécier d’un point de vue qualitatif et quantitatif la veine utérine, l’arcade utéro-annexielle, la veine utérine et les rameaux communicants entre le côté gauche et droit du paramètre, on a injecté chez six cadavres de la résine époxy dans la veine et l’artère iliaque interne et dans la veine et l’artère ovarienne. Dans deux cas les organes génitaux ont été excisés avant l’injection et chez quatre ils ont été réséqués après l’injection et la polymérisation de la résine. Les spécimens ont été examiné macroscopiquement et les diamètres veineux ont été measurés digitalement. Les diamètres moyens obtenus sont: 3.38 mm pour la veine utérine, 3.71 mm pour l’arcade utéro-ovarienne, 3.49 mm pour la veine ovarienne, 3.71 pour les rameaux communicants. Les veines ont été complètement remplies et mieux examinées chez les quatre cadavres où on a injecté in situ. On a pu observer que l’arcade utéro-ovarienne est une veine importante ayant un diamètre similaire avec les veines ovarienne et utérine. Il existe un important réseau d’anastomoses veineuses entre le paramètre gauche et droit et les trompes sont drainées par trois veines: tubaire interne, médiane, externe. La congestion veineuse pelvienne a été présente dans deux cas: une fois dans la région de la veine utérine gauche, une fois dans la région de la veine ovarienne gauche. Sachant que les diamètres des veines utérine, ovarienne et arcade utéro-ovarienne sont similaries, on pense qu’il n’existe pas de direction préférentielle de drainage veineux et que les veines des organes génitaux internes se comportent comme un bassin veineux. La congestion peut affecter des sites limités qui pourraient être traités localement. We described the anatomy of the utero-ovarian venous system in the adult postmenopausal female cadaver using casts and the von Hagen’s plastination technique. To qualitatively and quantitatively evaluate the Uterine vein, the utero-ovarian arcade, the ovarian vein and the ramus communicans between left and right parametrium, we injected epoxy resin into the internal iliac vein and artery and the ovarian vein and artery in six cadavers. In two cases the internal genital organs were excised before injection and in the other four after the injection and polymerization of the resin. All specimens were macroscopically examined and venous diameters were digitally measured, the average values of the measured diameters being 3.38 mm for the Uterine vein, 3.71 mm for the utero-ovarian arcade, 3.49 mm for the ovarian vein, and 3.71 mm for the ramus communicans. The Veins were completely filled out and could be best examined in the four cases where in situ injection was performed. We could observe that the utero-ovarian arcade is an important vein with a caliber similar to that of the ovarian and Uterine Veins, that there is an impressive network of venous anastomoses between the left and right parametrium and that the fallopian tubes are drained by three separate Veins: internal, median, and external tubal Veins. Pelvic venous congestion was present in two specimens; in one of them it was located in the region of the left Uterine vein and in the other one in the region of the left ovarian vein. Given the similar average diameters of the Uterine vein, ovarian vein, and utero-ovarian arcade, we assume that there is no preferential Uterine or ovarian venous drainage and that the Veins of the internal genital organs are behaving like a venous pool. Nevertheless, congestion can affect limited sites, which can and should be treated locally.
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Quantitative evaluation of the utero-ovarian venous pattern in the adult human female cadaver with plastination.
World journal of surgery, 2004Co-Authors: Mircea L. Pavkov, Jürgen Koebke, Hans -p. Notermans, Jost BrökelmannAbstract:We described the anatomy of the utero-ovarian venous system in the adult postmenopausal female cadaver using casts and the von Hagen’s plastination technique. To qualitatively and quantitatively evaluate the Uterine vein, the utero-ovarian arcade, the ovarian vein and the ramus communicans between left and right parametrium, we injected epoxy resin into the internal iliac vein and artery and the ovarian vein and artery in six cadavers. In two cases the internal genital organs were excised before injection and in the other four after the injection and polymerization of the resin. All specimens were macroscopically examined and venous diameters were digitally measured, the average values of the measured diameters being 3.38 mm for the Uterine vein, 3.71 mm for the utero-ovarian arcade, 3.49 mm for the ovarian vein, and 3.71 mm for the ramus communicans. The Veins were completely filled out and could be best examined in the four cases where in situ injection was performed. We could observe that the utero-ovarian arcade is an important vein with a caliber similar to that of the ovarian and Uterine Veins, that there is an impressive network of venous anastomoses between the left and right parametrium and that the fallopian tubes are drained by three separate Veins: internal, median, and external tubal Veins. Pelvic venous congestion was present in two specimens; in one of them it was located in the region of the left Uterine vein and in the other one in the region of the left ovarian vein. Given the similar average diameters of the Uterine vein, ovarian vein, and utero-ovarian arcade, we assume that there is no preferential Uterine or ovarian venous drainage and that the Veins of the internal genital organs are behaving like a venous pool. Nevertheless, congestion can affect limited sites, which can and should be treated locally.
John R. Higgins - One of the best experts on this subject based on the ideXlab platform.
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Circulating vascular cell adhesion molecule–1 in pre-eclampsia, gestational hypertension, and normal pregnancy: Evidence of selective dysregulation of vascular cell adhesion molecule–1 homeostasis in pre-eclampsia
American Journal of Obstetrics and Gynecology, 1998Co-Authors: John R. Higgins, Aikaterina Papayianni, Hugh R. Brady, Michael R. N. Darling, J. Joseph WalsheAbstract:Abstract OBJECTIVE: Our purpose was to investigate circulating levels of vascular cell adhesion molecule–1 in the peripheral and uteroplacental circulations during normotensive and hypertensive pregnancies. STUDY DESIGN: This prospective observational study involved 2 patient groups. Group 1 consisted of 22 women with pre-eclampsia and 30 normotensive women followed up longitudinally through pregnancy and post partum. There were an additional 13 women with established gestational hypertension. Group 2 consisted of 20 women with established pre-eclampsia and 19 normotensive control subjects undergoing cesarean delivery. Plasma levels of vascular cell adhesion molecule–1 were measured in blood drawn from the antecubital vein (group 1) and from both the antecubital and Uterine Veins (group 2). Data were analyzed by analysis of variance. RESULTS: In group 1 vascular cell adhesion molecule–1 levels did not change significantly throughout normal pregnancy and post partum. Women with established pre-eclampsia had increased vascular cell adhesion molecule–1 levels compared with the normotensive pregnancy group ( P = .01). Vascular cell adhesion molecule–1 levels were not elevated in women with established gestational hypertension. In group 2 significantly higher levels of vascular cell adhesion molecule–1 were detected in the uteroplacental ( P P P = .06). In contrast to vascular cell adhesion molecule–1, circulating levels of E-selectin and intercellular adhesion molecule–1, other major leukocyte adhesion molecules expressed by the endothelium, were not different in pre-eclamptic and normotensive pregnancies. CONCLUSION: Established pre-eclampsia is characterized by selective dysregulation of vascular cell adhesion molecule–1 homeostasis. This event is not an early preclinical feature of pre-eclampsia, does not persist post partum, is not a feature of nonproteinuric gestational hypertension, and is not observed with other major leukocyte adhesion molecules. Induction of vascular cell adhesion molecule–1 expression in pre-eclampsia may contribute to leukocyte-mediated tissue injury in this condition or may reflect perturbation of other, previously unrecognized, functions of this molecule in pregnancy. (Am J Obstet Gynecol 1998;179:464-9.)
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Hemostasis in the uteroplacental and peripheral circulations in normotensive and pre-eclamptic pregnancies☆☆☆★★★♢
American journal of obstetrics and gynecology, 1998Co-Authors: John R. Higgins, Michael R. N. Darling, J. Joseph Walshe, Lucy A. Norris, John BonnarAbstract:Abstract OBJECTIVE: Our purpose was to determine the hemostatic changes in the uteroplacental and peripheral circulations in normotensive and pre-eclamptic pregnancies. STUDY DESIGN: This prospective, observational study involved 2 patient groups. Group 1 consisted of 30 normotensive women and 22 women with pre-eclampsia who were followed up longitudinally through pregnancy and post partum. Group 2 consisted of 20 women with established pre-eclampsia and 19 normotensive control subjects, all undergoing cesarean section. Plasma levels of thrombin–antithrombin III complex, soluble fibrin, plasmin–α 2 -antiplasmin complex, and fibrin-degradation product (D-dimer) were measured in blood drawn from the antecubital vein (group 1) and from both the antecubital and Uterine Veins (group 2). Data were analyzed by analysis of variance. RESULTS: In group 1 levels of thrombin–antithrombin III complex, soluble fibrin, and fibrin-degradation product were significantly higher during normal pregnancy than at 6 weeks post partum. Plasmin–α 2 -antiplasmin complex levels did not change. No differences between the pre-eclamptic and normotensive pregnancy groups were found for any of the hemostatic markers. In group 2 normotensive women undergoing cesarean section, thrombin–antithrombin III complex and soluble fibrin levels were significantly higher in the Uterine vein than in the antecubital vein. In group 2 women with pre-eclampsia, thrombin–antithrombin III complex and fibrin-degradation product levels were significantly higher in the Uterine vein than in the antecubital vein. In addition, plasmin–α 2 -antiplasmin complex and fibrin-degradation product levels were higher and soluble fibrin levels were lower in the Uterine vein in the pre-eclamptic group than in the normotensive group. CONCLUSION: Both the coagulation and fibrinolytic systems are activated during normal pregnancy. Activation of these systems is more marked in the uteroplacental circulation than in the systemic circulation in both normotensive and pre-eclamptic pregnancies. An abnormal pattern of hemostasis occurs in the uteroplacental circulation in pre-eclampsia. (Am J Obstet Gynecol 1998;179:520-6.)
