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Karaçetin Serkan - One of the best experts on this subject based on the ideXlab platform.
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Protective effects of l-carnitine on x irradiationinduced Uterus injury via antioxidant and anti-inflammatory pathways
Histoloji ve Embriyoloji Anabilim Dalı, 2020Co-Authors: Karaçetin SerkanAbstract:Bu çalışmanın amacı total vücut ışınlaması sonrası, sıçanların Uterusendometriyum tabakasında meydana gelen hasara karşı L-karnitin’in antioksidan, antiinflamatuar ve radyoprotektif etkilerini araştırmaktır. Çalışmamızda 30 adet Wistar albino sıçan, kontrol, radyasyon 6 saat, radyasyon 4 gün, radyasyon 6 saat+L-karnitin, radyasyon 4 gün+L-karnitin olmak üzere 5 gruba ayrıldı. Kontrol grubuna deney süresince intraperitonal yolla serum fizyolojik uygulandı. Radyasyon 6 saat ve radyasyon 4 gün gruplarına tüm vücut tek doz 8,3 Gy X ışını uygulandı. Bu gruplar ışınlama sonrasında 6. saat ve 4. günde sırasıyla uygun anestezi altında sakrifiye edildi. Radyasyon 6 saat+L-karnitin ve radyasyon 4 gün+L-karnitin gruplarına aynı şekilde radyasyon uygulaması ile birlikte günlük 200mg/kg dozda L-karnitin intraperitonal yolla uygulandı. Bu gruplar da ışınlama sonrasındaki 6. saat ve 4. günde sırasıyla sakrifiye edildi. Deneklerden alınan Uterus biyopsi örnekleri histopatolojik, immünohistokimyasal ve biyokimyasal analizler için uygun işlemlerden geçirildi. Radyasyona bağlı olarak endometriyum yüzey ve bez epitel hücrelerinde düzleşme, derin bezlerde kayıplar olduğu görüldü. L-karnitin tedavisi ile bu değişikliklerin kısmen önlendiği saptandı. Radyasyon gruplarında Uterus dokusundaki PARP-1, IL- 1β, IL-6, TNF–α ve Nf–κBekspresyonu, kontrol ve L-Karnitin ile tedavi edilen gruplara kıyasla belirgin şekilde arttığı görüldü. Radyasyona bağlı açığa çıkan morfolojik değişiklikleri büyük oranda serbest oksijen radikallerinin oluşturduğu, oksidatif stres indeksi ile belirlendi. Total oksidan durum belirteci radyasyon gruplarında yüksek çıkarken, kontrol ve L-Karnitin tedavili gruplarda düşük olduğu saptandı. Total antioksidan durum belirtecinin özellikle L-karnitin tedavili gruplarda yüksek olması, L-karnitin’in antioksidan özelliği olduğunu gösterdi. Çalışmamızda elde ettiğimiz bulgular ışığında, radyasyon maruziyeti sonucunda oluşan Uterus hasarına karşı L-Karnitin’in koruyucu etkisi olabileceği tespit edildiThe aim of this study to investigate L-carnitine, with antioxidant, anti inflammatory and radioprotective effects against degenerasyon of uterine endometrial surface and gland epitelium after total body irradiation. In our study thirty Wistar albino rats divided into five groups as control, radiation 6 hour (Rd6h), radiation 4 day (Rd4d), radiation 6 hour+L-carnitine (Rd6h+LC), radiation 4 day+L-carnitine (Rd4d + LC). The control group received physiological saline intraperitoneally. Rd6h and Rd4d received whole-body X-irradiation of 8.3 Gy as a single dose. These groups were sacrificed at the 6th hour and 4th day after irradiation with anesthesia, respectively.The Rd6h+LC and Rd4d+LC groups received the same dose irradiation plus a daily dose of 200 mg/kg L-carnitine intraperitoneally. These groups were sacrificed at the 6th hour and 4th day after irradiation too. Uterus Biopsy samples from rats were processed for histopathological, immunohistochemical and biochemical analysis. Endometrial surface and gland epithelium cells were flattened and deep glands werelost due to radiation. These changes were partially prevented by L-Carnitine treatment. PARP-1, IL-1β, IL-6, TNF–α ve Nf–κB expression levels in radiationgroups were significantly increased compared to control and L-Carnitine treatedgroups. The morphological changes due to radiation were determined by oxidative stress index, which is mainly composed of free oxygen radicals. While total oxidant status marker was found to be high in radiation groups, it was found to be low in control and L-Carnitine treatment groups. The high total antioxidant status markers in L-Carnitine treated groups showed that L-Carnitine had antioxidant properties. In our study, we found that L-Carnitine may have a protective effect against uterine damage caused by radiation
Paula Serrano - One of the best experts on this subject based on the ideXlab platform.
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Carcinoma of the cervix complicating a genital prolapse
2020Co-Authors: Catarina Pardal, Catia Correia, Paula SerranoAbstract:SUMMARY Although uterine prolapse and carcinoma of the uterine cervix are not rare events, their association is very uncommon. The treatment of cervical cancer has been protocolled, but the management of uterovaginal prolapse associated with carcinoma of the cervix is not standardised and therapy strategies vary considerably among authors. Our case reports a 74-year-old patient, admitted to the emergency department with an ulcerated prolapsed Uterus. Biopsy of the cervical lesion confirmed a squamous-cell carcinoma. The patient underwent vaginal hysterectomy plus open bilateral iliopelvic lymphadenectomy complemented with radiotherapy with quimiosensibilisation. With this aggressive treatment approach, there was progression of the disease. The authors believe that this case typiaddition to the few published reports. BACKGROUN
Catarina Pardal - One of the best experts on this subject based on the ideXlab platform.
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Carcinoma of the cervix complicating a genital prolapse
2020Co-Authors: Catarina Pardal, Catia Correia, Paula SerranoAbstract:SUMMARY Although uterine prolapse and carcinoma of the uterine cervix are not rare events, their association is very uncommon. The treatment of cervical cancer has been protocolled, but the management of uterovaginal prolapse associated with carcinoma of the cervix is not standardised and therapy strategies vary considerably among authors. Our case reports a 74-year-old patient, admitted to the emergency department with an ulcerated prolapsed Uterus. Biopsy of the cervical lesion confirmed a squamous-cell carcinoma. The patient underwent vaginal hysterectomy plus open bilateral iliopelvic lymphadenectomy complemented with radiotherapy with quimiosensibilisation. With this aggressive treatment approach, there was progression of the disease. The authors believe that this case typiaddition to the few published reports. BACKGROUN
Hinrichs K - One of the best experts on this subject based on the ideXlab platform.
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Application of preimplantation genetic diagnosis in equine blastocysts
Asociación Peruana de Reproducción Animal, 2016Co-Authors: Hinrichs KAbstract:Pre-implantation genetic diagnosis (PGD) is a procedure used to screen in vitroproduced embryos or embryos recovered after uterine flush to determine genetic traits by DNA testing prior to transfer into the Uterus. Biopsy methods to obtain a sample of cells for genetic analysis before implantation have been successful in small embryos (morulae and blastocysts < 300 µm diameter), but this technique was initially unsuccessful in large embryos (expanded blastocysts > 300 µm diameter). The successful Biopsy of expanded equine blastocysts via micromanipulation, with subsequent normal pregnancy rates, was first reported in 2010. Direct PCR may be performed when evaluating only one gene, such as for embryo sexing, while whole genome amplification is effective for subsequent multiplex PCR of multiple genes
Catia Correia - One of the best experts on this subject based on the ideXlab platform.
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Carcinoma of the cervix complicating a genital prolapse
2020Co-Authors: Catarina Pardal, Catia Correia, Paula SerranoAbstract:SUMMARY Although uterine prolapse and carcinoma of the uterine cervix are not rare events, their association is very uncommon. The treatment of cervical cancer has been protocolled, but the management of uterovaginal prolapse associated with carcinoma of the cervix is not standardised and therapy strategies vary considerably among authors. Our case reports a 74-year-old patient, admitted to the emergency department with an ulcerated prolapsed Uterus. Biopsy of the cervical lesion confirmed a squamous-cell carcinoma. The patient underwent vaginal hysterectomy plus open bilateral iliopelvic lymphadenectomy complemented with radiotherapy with quimiosensibilisation. With this aggressive treatment approach, there was progression of the disease. The authors believe that this case typiaddition to the few published reports. BACKGROUN
