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Lutfi Behcet - One of the best experts on this subject based on the ideXlab platform.
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bioactivity guided isolation of flavonoids from cynanchum acutum l subsp sibiricum willd rech f and investigation of their antiproliferative activity
Natural Product Research, 2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
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Bioactivity–guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity
2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
Ilyas Yildiz - One of the best experts on this subject based on the ideXlab platform.
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bioactivity guided isolation of flavonoids from cynanchum acutum l subsp sibiricum willd rech f and investigation of their antiproliferative activity
Natural Product Research, 2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
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Bioactivity–guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity
2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
Ibrahim Demirtas - One of the best experts on this subject based on the ideXlab platform.
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Antiproliferative and Antioxidant Activities of Two Extracts of the Plant Species Euphorbia dendroides L.
MDPI AG, 2018Co-Authors: Agena Ghout, Ibrahim Demirtas, Amar Zellagui, Noureddine Gherraf, Yaglioglu Ayse Sahin, Meriem Boukhenaf, Mesbah Lahouel, Gema Nieto, Salah AkkalAbstract:Background: These days, the desire for naturally occurring antioxidants has significantly increased, especially for use in foodstuffs, cosmetics, and pharmaceutical products, to replace synthetic antioxidants that are regularly constrained due to their carcinogenicity. Methods: The study in hand aimed to appraise the antioxidant effect of two Euphorbia dendroides extracts using reducing power, anti-peroxidation, and DPPH (1,1 Diphenyl 2 Pycril Hydrazil) scavenging essays, in addition to the anticancer activity against two tumor cell lines, namely C6 (rat brain tumor)cells, and Hela (human Uterus Carcinoma)cell lines. Results: The results indicated that the ethyl acetate extract exhibited antiradical activity of 29.49%, higher than that of n-butanol extract (18.06%) at 100 µg/mL but much lower than that of gallic acid (78.21%).The ethyl acetate extract exhibits better reducing capacity and lipid peroxidation inhibitory activity compared to n-butanol extract but less than all tested standards. Moreover, the ethyl acetate extract was found to have an antiproliferative activity of more than 5-FU (5-fluoro-Uracil) against C6 cells at 250 µg/mL with IC50 and IC75 of 113.97, 119.49 µg/mL, respectively, and good cytotoxic activity against the Hela cell lines at the same concentration. The HPLC-TOF-MS (high performance liquid chromatography-Time-of-flight-Mass Spectrometry) analyses exposed the presence of various compounds, among which Gallic and Chlorogenic acids functioned as major compounds. Conclusions: The two extracts exhibited moderate anticancer abilities and behaved somewhat as average antioxidant agents. Based on the total phenolics and flavonoids contents, as well as HPLC results, it could be concluded that antiproliferative and antioxidant activities depend upon the content of different phenolics and flavonoids
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bioactivity guided isolation of flavonoids from cynanchum acutum l subsp sibiricum willd rech f and investigation of their antiproliferative activity
Natural Product Research, 2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
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Bioactivity–guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity
2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
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Studies on the Antioxidant and Antiproliferative Potentials of Cirsium arvense subsp. vestitum
Journal of Food Biochemistry, 2016Co-Authors: Ibrahim Demirtas, Ali Rıza Tüfekçi, Ayse Sahin Yaglioglu, Mahfuz ElmastaşAbstract:The antioxidant (DPPH, superoxide anion radical scavenging and FRAP) and antiproliferative activities against Vero cells (african green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human Uterus Carcinoma) of Cirsium arvense (CA) of all parts [root (Kinghorn et al. 2004), stem (CAS), flower (CAF) and leaves] and isolated compounds were investigated in vitro. CAR was shown to have the highest antiproliferative activity against HeLa and C6 cell lines as compared to other parts. A flavon glucoside (linarin), a steroid glucoside (daucosterin), a steroid (stigmasterol) and a new benzymidazole molecule (E−6-(3-(5,6-dimethoxy-1H-benzo[d] imidazol-2-yl)allyloxy)−5-(hydroxymethyl)-tetrahydro-2H-pyran-2,3,4-triol) were isolated from the CAR. The structures of these molecules were determined by the 13C (carbon) and 1H (proton)- Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared Spectroscopy (FT-IR), and Gas Chromatography-Mass Spectrometry (GC-MS) techniques. CAR for C6 cell line, CAS for HeLa and Vero cell line were observed the highest antiproliferative activity. In addition, CAR showed the stronger DPPH activity than α-tocopherol and BHT at high concentrations. The FRAP and superoxide anion radical scavenging activities of CAR (Fr-7) were found to be higher than BHT. Practical Applications Cirsium species are considered as agricultural weed among villagers in Turkey and are used as alternative medicinal plant for years. Studies have shown that these species are rich with regard to bioactive seconder metabolites. There is no phytochemical/bioactivity study about this plant until now. Our findings showed that the extracts of this plant possessed potent antioxidant activities. Four compounds, one of which is a new one to literature, were isolated and characterized. Finally, Cirsium arvense, being considered as an invader plant in agricultural fields, can be evaluated as a good source of antioxidant products.
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Activity of Trans-Tiliroside Compound from an Endemic Desert Species
2016Co-Authors: Labib Noman, Ayse Sahin Yaglioglu, Ibrahim Demirtas, Amar Zellagui, Rhouati SalahAbstract:Essential oil components of the aerial parts of Thymelaea microphylla was subjected to evaluate the antimicrobial activity by disc diffusion method and tested against Gram-positive and Gram-negative bacteria and showed a high antibacterial activity against E.coli and Staphylococcus aureus at 2000μg /ml. The antiproliferative activity of fraction (7) from Thymelaea microphylla showed significant inhibitory activity on HeLa cells and moderate antiproliferative activity of trans-tiliroside compound was investigated in vitro on HeLa cells (human Uterus Carcinoma) activity and C6 cells (rat brain tumor)
Ozkan Sen - One of the best experts on this subject based on the ideXlab platform.
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bioactivity guided isolation of flavonoids from cynanchum acutum l subsp sibiricum willd rech f and investigation of their antiproliferative activity
Natural Product Research, 2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
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Bioactivity–guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity
2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
Ramazan Erenler - One of the best experts on this subject based on the ideXlab platform.
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bioactivity guided isolation of flavonoids from cynanchum acutum l subsp sibiricum willd rech f and investigation of their antiproliferative activity
Natural Product Research, 2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
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Bioactivity–guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity
2017Co-Authors: Ilyas Yildiz, Ibrahim Demirtas, Ramazan Erenler, Ozkan Sen, Lutfi BehcetAbstract:Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human Uterus Carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.
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bioactivity guided isolation of antiproliferative sesquiterpene lactones from centaurea solstitialis l ssp solstitialis
Combinatorial Chemistry & High Throughput Screening, 2016Co-Authors: Ramazan Erenler, Ayse Sahin Yaglioglu, Ibrahim DemirtasAbstract:Centaurea solstitialis L. ssp. solstitialis (CSS) has been used as medicine for various diseases. In this work, root, stem and flower parts of the plant were separately extracted with methanol to execute bioassay-guided isolation. Antiproliferative activities of each extracts on C6 cells (Rat Brain tumor cells) and HeLa cells (human Uterus Carcinoma) were investigated in vitro. The methanol extract of stem exhibited the most antiproliferative activity therefore isolation of active compounds was carried out for stem of the plant. Methanol extract of stem was boiled at 97 °C for 2 hours in water and then hexane and ethyl acetate were extracted sequentially. Solstitialin A 1 and 15-dechloro-15-hydroxychlorojanerin 2 were isolated from ethyl acetate extract by column chromatography and identified by spectroscopic techniques. Solstitialin A 1 was isolated from CSS and 15-dechloro-15-hydroxychlorojanerin 2 was isolated from Saussurea lipschitz and Rhaponticum pulchrum previously. These two compounds exhibited very high antiproliferative activity on C6 and HeLa cells. IC 50 and IC 75 values of compound 1 were obtained as 10.78 and 53.65 against C6 cell and as 48.78 and 68.52 against HeLa, respectively. IC 50 and IC 75 values of compound 2 were determined as 432.43 and 109.79 against C6 cell.
