The Experts below are selected from a list of 693 Experts worldwide ranked by ideXlab platform
Yuping Tang - One of the best experts on this subject based on the ideXlab platform.
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Bio-activity evaluation of Qinlian Siwu decoction on inhibiting mice uterine Contraction in vitro and its components analysis
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2010Co-Authors: Chun-mei Liu, Jin-ao Duan, Yongqing Hua, Yuping Tang, Erxin Shang, Anwei DingAbstract:Objective To evaluate the bio-activity of Qinlian Siwu decoction on in vitro Uterus Contraction model and exploit the relationship between chemical components and the bio-activity. Method The samples were prepared by macroporous adsorptive resins. The in vitro Uterus Contraction model was adopted to appraise the bio-activities of Qinlian Siwu decoction and its different separated fractions. HPLC-DAD- ESI -MS method was applied to analyze and identify the components in the fraction QL-3. Result It was found that five active fractions (QL-1, QL-3, QL-5, QL-7 and QL-11) were separated from Qinlian Siwu decoction, mainly contributed to the observed antagonismto the Contraction of the mouse Uterus. 28 compounds in the fraction QL-3 were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, chuanxiongzine hydrochloridum, vanillic acid, caffeic acid, paeoniflorin, berberastine, albiflorin, tetrahydropalmatine, coptisine, jatrorrhizine, leonuride, worenine, ferulic acid, palmatine, berberine, scutellarin, baicalin-7-0-glucoside, baicalin, rehmannioside C, wogonoside, chrysin-7-glucuronide, ttetuin, baicalein, wogonin and oroxylin-A. Conclusion In vitro inhibiting the Contraction of the isolated mouse uterine of Qinlian Siwu decoction was mainly attributed to the fraction QL-1 and QL-3. The active fractions (QL-5, QL-7 and QL-11) were obtained from QL-3 on the macroporous adsorptive resins by the gradient elution using ethanol.
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Effects of Xiang-Fu-Si-Wu Decoction and its main components for dysmenorrhea on Uterus Contraction
Journal of ethnopharmacology, 2010Co-Authors: Pei Liu, Jin-ao Duan, Yongqing Hua, Yuping Tang, Xin YaoAbstract:Ethnopharmacological relevance Xiang-Fu-Si-Wu Decoction has been widely used to treat blood stasis syndromes in gynecology diseases, such as primary dysmenorrhea in clinical practice for hundreds of years and show great efficacy. The efficient components and mechanism of action on Uterus Contraction were seldom reported. Aim of the study The present study was conducted to evaluate the inhibitory effects of active fractions and its main bioactive components of Xiang-Fu-Si-Wu Decoction on uterine Contraction. Materials and methods Model of non-pregnant mice uterine Contraction induced by oxytocin was used to evaluate activity. Levels of Ca2+ and nitric oxide (NO) in primary dysmenorrheal model mice Uterus were also been detected. Components in active fraction were identified and quantified by HPLC-DAD. Results and conclusions It was found the active fraction of Xiang-Fu-Si-Wu Decoction may become potential Ca2+ channel blocking agents. Alkaloids like berberine were main active components in bioactive fraction of Xiang-Fu-Si-Wu Decoction for dysmenorrhea on Uterus Contraction.
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Inhibitory effects of active fraction and its main components of Shaofu Zhuyu decoction on Uterus Contraction.
The American journal of Chinese medicine, 2010Co-Authors: Yongqing Hua, Jin-ao Duan, Erxin Shang, Wei Zhou, Yuping TangAbstract:Shaofu Zhuyu decoction is a famous formula for treating primary dysmenorrhea in China since the Qing dynasty. In this paper, the inhibitory effects of active-guided fraction and its main bioactive components of Shaofu Zhuyu decoction on a model of non-pregnant mice uterine Contraction induced by oxytocin in vitro were investigated. Qualitative and quantitative chemical analyses were used to correlate the chemical composition of active fraction with the spasmolytic effects. Seven ingredients in the active fraction were identified and quantified by HPLC-DAD. Three ingredients, ferulic acid, vanillic acid, and typhaneoside, were evaluated for their effects on mice isolated uterine Contraction induced by oxytocin in vitro. The ED(50) of them were 63.0 microg/ml, 57.6 microg/ml, 109.7 microg/ml, respectively. Furthermore, the inhibitory activity of the combination of these three compounds was prior to the fraction and seven compounds group. The ED(50) was 65.5 microg/ml. The data stated that ferulic acid, vanillic acid, and typhaneoside were possibly the main active components in the bioactive fraction of Shaofu Zhuyu decoction. The study also implied that Shaofu Zhuyu decoction may have direct inhibitory effects on the contractility of the mice Uterus and justified the traditional use of the prescription for treating the uterine cramping associated dysmenorrhea.
Yongqing Hua - One of the best experts on this subject based on the ideXlab platform.
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Bio-activity evaluation of Qinlian Siwu decoction on inhibiting mice uterine Contraction in vitro and its components analysis
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2010Co-Authors: Chun-mei Liu, Jin-ao Duan, Yongqing Hua, Yuping Tang, Erxin Shang, Anwei DingAbstract:Objective To evaluate the bio-activity of Qinlian Siwu decoction on in vitro Uterus Contraction model and exploit the relationship between chemical components and the bio-activity. Method The samples were prepared by macroporous adsorptive resins. The in vitro Uterus Contraction model was adopted to appraise the bio-activities of Qinlian Siwu decoction and its different separated fractions. HPLC-DAD- ESI -MS method was applied to analyze and identify the components in the fraction QL-3. Result It was found that five active fractions (QL-1, QL-3, QL-5, QL-7 and QL-11) were separated from Qinlian Siwu decoction, mainly contributed to the observed antagonismto the Contraction of the mouse Uterus. 28 compounds in the fraction QL-3 were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, chuanxiongzine hydrochloridum, vanillic acid, caffeic acid, paeoniflorin, berberastine, albiflorin, tetrahydropalmatine, coptisine, jatrorrhizine, leonuride, worenine, ferulic acid, palmatine, berberine, scutellarin, baicalin-7-0-glucoside, baicalin, rehmannioside C, wogonoside, chrysin-7-glucuronide, ttetuin, baicalein, wogonin and oroxylin-A. Conclusion In vitro inhibiting the Contraction of the isolated mouse uterine of Qinlian Siwu decoction was mainly attributed to the fraction QL-1 and QL-3. The active fractions (QL-5, QL-7 and QL-11) were obtained from QL-3 on the macroporous adsorptive resins by the gradient elution using ethanol.
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Effects of Xiang-Fu-Si-Wu Decoction and its main components for dysmenorrhea on Uterus Contraction
Journal of ethnopharmacology, 2010Co-Authors: Pei Liu, Jin-ao Duan, Yongqing Hua, Yuping Tang, Xin YaoAbstract:Ethnopharmacological relevance Xiang-Fu-Si-Wu Decoction has been widely used to treat blood stasis syndromes in gynecology diseases, such as primary dysmenorrhea in clinical practice for hundreds of years and show great efficacy. The efficient components and mechanism of action on Uterus Contraction were seldom reported. Aim of the study The present study was conducted to evaluate the inhibitory effects of active fractions and its main bioactive components of Xiang-Fu-Si-Wu Decoction on uterine Contraction. Materials and methods Model of non-pregnant mice uterine Contraction induced by oxytocin was used to evaluate activity. Levels of Ca2+ and nitric oxide (NO) in primary dysmenorrheal model mice Uterus were also been detected. Components in active fraction were identified and quantified by HPLC-DAD. Results and conclusions It was found the active fraction of Xiang-Fu-Si-Wu Decoction may become potential Ca2+ channel blocking agents. Alkaloids like berberine were main active components in bioactive fraction of Xiang-Fu-Si-Wu Decoction for dysmenorrhea on Uterus Contraction.
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Inhibitory effects of active fraction and its main components of Shaofu Zhuyu decoction on Uterus Contraction.
The American journal of Chinese medicine, 2010Co-Authors: Yongqing Hua, Jin-ao Duan, Erxin Shang, Wei Zhou, Yuping TangAbstract:Shaofu Zhuyu decoction is a famous formula for treating primary dysmenorrhea in China since the Qing dynasty. In this paper, the inhibitory effects of active-guided fraction and its main bioactive components of Shaofu Zhuyu decoction on a model of non-pregnant mice uterine Contraction induced by oxytocin in vitro were investigated. Qualitative and quantitative chemical analyses were used to correlate the chemical composition of active fraction with the spasmolytic effects. Seven ingredients in the active fraction were identified and quantified by HPLC-DAD. Three ingredients, ferulic acid, vanillic acid, and typhaneoside, were evaluated for their effects on mice isolated uterine Contraction induced by oxytocin in vitro. The ED(50) of them were 63.0 microg/ml, 57.6 microg/ml, 109.7 microg/ml, respectively. Furthermore, the inhibitory activity of the combination of these three compounds was prior to the fraction and seven compounds group. The ED(50) was 65.5 microg/ml. The data stated that ferulic acid, vanillic acid, and typhaneoside were possibly the main active components in the bioactive fraction of Shaofu Zhuyu decoction. The study also implied that Shaofu Zhuyu decoction may have direct inhibitory effects on the contractility of the mice Uterus and justified the traditional use of the prescription for treating the uterine cramping associated dysmenorrhea.
Jin-ao Duan - One of the best experts on this subject based on the ideXlab platform.
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Bio-activity evaluation of Qinlian Siwu decoction on inhibiting mice uterine Contraction in vitro and its components analysis
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2010Co-Authors: Chun-mei Liu, Jin-ao Duan, Yongqing Hua, Yuping Tang, Erxin Shang, Anwei DingAbstract:Objective To evaluate the bio-activity of Qinlian Siwu decoction on in vitro Uterus Contraction model and exploit the relationship between chemical components and the bio-activity. Method The samples were prepared by macroporous adsorptive resins. The in vitro Uterus Contraction model was adopted to appraise the bio-activities of Qinlian Siwu decoction and its different separated fractions. HPLC-DAD- ESI -MS method was applied to analyze and identify the components in the fraction QL-3. Result It was found that five active fractions (QL-1, QL-3, QL-5, QL-7 and QL-11) were separated from Qinlian Siwu decoction, mainly contributed to the observed antagonismto the Contraction of the mouse Uterus. 28 compounds in the fraction QL-3 were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, chuanxiongzine hydrochloridum, vanillic acid, caffeic acid, paeoniflorin, berberastine, albiflorin, tetrahydropalmatine, coptisine, jatrorrhizine, leonuride, worenine, ferulic acid, palmatine, berberine, scutellarin, baicalin-7-0-glucoside, baicalin, rehmannioside C, wogonoside, chrysin-7-glucuronide, ttetuin, baicalein, wogonin and oroxylin-A. Conclusion In vitro inhibiting the Contraction of the isolated mouse uterine of Qinlian Siwu decoction was mainly attributed to the fraction QL-1 and QL-3. The active fractions (QL-5, QL-7 and QL-11) were obtained from QL-3 on the macroporous adsorptive resins by the gradient elution using ethanol.
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Effects of Xiang-Fu-Si-Wu Decoction and its main components for dysmenorrhea on Uterus Contraction
Journal of ethnopharmacology, 2010Co-Authors: Pei Liu, Jin-ao Duan, Yongqing Hua, Yuping Tang, Xin YaoAbstract:Ethnopharmacological relevance Xiang-Fu-Si-Wu Decoction has been widely used to treat blood stasis syndromes in gynecology diseases, such as primary dysmenorrhea in clinical practice for hundreds of years and show great efficacy. The efficient components and mechanism of action on Uterus Contraction were seldom reported. Aim of the study The present study was conducted to evaluate the inhibitory effects of active fractions and its main bioactive components of Xiang-Fu-Si-Wu Decoction on uterine Contraction. Materials and methods Model of non-pregnant mice uterine Contraction induced by oxytocin was used to evaluate activity. Levels of Ca2+ and nitric oxide (NO) in primary dysmenorrheal model mice Uterus were also been detected. Components in active fraction were identified and quantified by HPLC-DAD. Results and conclusions It was found the active fraction of Xiang-Fu-Si-Wu Decoction may become potential Ca2+ channel blocking agents. Alkaloids like berberine were main active components in bioactive fraction of Xiang-Fu-Si-Wu Decoction for dysmenorrhea on Uterus Contraction.
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Inhibitory effects of active fraction and its main components of Shaofu Zhuyu decoction on Uterus Contraction.
The American journal of Chinese medicine, 2010Co-Authors: Yongqing Hua, Jin-ao Duan, Erxin Shang, Wei Zhou, Yuping TangAbstract:Shaofu Zhuyu decoction is a famous formula for treating primary dysmenorrhea in China since the Qing dynasty. In this paper, the inhibitory effects of active-guided fraction and its main bioactive components of Shaofu Zhuyu decoction on a model of non-pregnant mice uterine Contraction induced by oxytocin in vitro were investigated. Qualitative and quantitative chemical analyses were used to correlate the chemical composition of active fraction with the spasmolytic effects. Seven ingredients in the active fraction were identified and quantified by HPLC-DAD. Three ingredients, ferulic acid, vanillic acid, and typhaneoside, were evaluated for their effects on mice isolated uterine Contraction induced by oxytocin in vitro. The ED(50) of them were 63.0 microg/ml, 57.6 microg/ml, 109.7 microg/ml, respectively. Furthermore, the inhibitory activity of the combination of these three compounds was prior to the fraction and seven compounds group. The ED(50) was 65.5 microg/ml. The data stated that ferulic acid, vanillic acid, and typhaneoside were possibly the main active components in the bioactive fraction of Shaofu Zhuyu decoction. The study also implied that Shaofu Zhuyu decoction may have direct inhibitory effects on the contractility of the mice Uterus and justified the traditional use of the prescription for treating the uterine cramping associated dysmenorrhea.
Seyed Ebrahim Sajjadi - One of the best experts on this subject based on the ideXlab platform.
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Effect of Matricaria chamomilla hydro-alcoholic and flavonoids rich extracts on rat isolated Uterus
Journal of Herbmed Pharmacology, 2020Co-Authors: Hassan Sadraei, Gholam-reza Asghari, Seyed Ebrahim Sajjadi, Majid KhaliliAbstract:Introduction: Pharmacological studies confirm antispasmodic activities of chamomile (Matricaria chamomilla) extract on intestinal smooth muscles and it has been suggested that chamomile increases Uterus tone, but so far there is no scientific studies which support this assumption. Therefore, this study was designed to determine spasmodic and spasmolytic activities of M. chamomilla extracts on rat isolated Uterus. Methods: Hydro-alcoholic extract of M. chamomilla was prepared by maceration technique. Flavonoids rich extract was prepared by liquid in liquid extraction technique. The spasmodic effects of the extracts were assessed on spontaneously contracting rat Uterus. The myorelaxant effect of M. chamomilla extracts was validated on isolated Uterus Contractions induced by KCl, acetylcholine (ACh), electrical field stimulation (EFS) and oxytocin. Results: Hydro-alcoholic extract of M. chamomilla (0.8 and 1.6 mg/mL) enhanced spontaneous movement of rat isolated Uterus smooth muscle suspended in an organ bath. On the other hand, flavonoids rich fraction only diminished Uterus contractile activities. Flavonoids rich extract of the plant at bath concentration ranges of 40 μg/mL to 400 μg/mL attenuated Uterus response to ACh, KCl, EFS and oxytocin. The hydro-alcoholic extract of M. chamomilla at higher concentration ranges (250 μg/mL to 1.5 mg/mL) inhibited Uterus Contractions induced by the above spasmogens. Conclusion: The present study confirms both spasmodic and spasmolytic activities M. chamomilla hydro-alcoholic extract. Therefore, medicinal use of the crude extract of M. chamomilla may initiate Uterus Contraction which could increase risk of spontaneous miscarriage or premature parturition.
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antispasmodic effect of osthole and prangos ferulacea extract on rat Uterus smooth muscle motility
Research in Pharmaceutical Sciences, 2012Co-Authors: H Sadraei, Yalda Shokoohinia, Seyed Ebrahim SajjadiAbstract:Several species of Prangos are traditionally used as emollient, carminative, tonic, anti-flatulent, anthelmintic and anti-thrombotic agents. Osthole, a coumarin isolated from Prangos, are believed to be responsible for some of its effects. In this research, relaxant effects of Prangos ferulacea extract and osthole on rat Uterus Contraction induced by KCl, acetylcholine (ACh), oxytocin and electrical field stimulation (EFS) was investigated and compared with atropine and salbutamol. P. ferulacea acetonic extract concentrationdependently relaxed uterine Contraction induced by KCl (IC50=13 ± 0.81 µg/ml), ACh (IC50=12 ± 1.38 µg/ml), oxytocin (IC50=16 ± 3.14 µg/ml) and EFS (IC50=11 ± 1.5 µg/ml). However, the extract at lower concentration (2.5 µg/ml) potentiated the EFS response. Osthole only had inhibitory effect on rat Uterus and its relaxant effect was observed at lower concentration in comparison with P. ferulacea extract. Osthole in a similar way inhibited the response to KCl (IC50=4 ± 0.13 µg/ml), ACh (IC50=4 ± 0.8 µg/ml), oxytocin (IC50=4 ± 0.8 µg/ml) and EFS (IC50=1.5 ± 0.5 µg/ml). Our results demonstrated that osthole acted directly on Uterus smooth muscle to induce relaxation, whereas P. ferulacea caused both Contraction and relaxation of rat uterine smooth muscle. The relaxation of osthole might be mediated through Ca 2+ channel blocking activity as it inhibited the response to KCl. Mechanisms other than Ca 2+ channel blocking appeared to be responsible for ACh relaxation effect of osthole.
Erxin Shang - One of the best experts on this subject based on the ideXlab platform.
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Bio-activity evaluation of Qinlian Siwu decoction on inhibiting mice uterine Contraction in vitro and its components analysis
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2010Co-Authors: Chun-mei Liu, Jin-ao Duan, Yongqing Hua, Yuping Tang, Erxin Shang, Anwei DingAbstract:Objective To evaluate the bio-activity of Qinlian Siwu decoction on in vitro Uterus Contraction model and exploit the relationship between chemical components and the bio-activity. Method The samples were prepared by macroporous adsorptive resins. The in vitro Uterus Contraction model was adopted to appraise the bio-activities of Qinlian Siwu decoction and its different separated fractions. HPLC-DAD- ESI -MS method was applied to analyze and identify the components in the fraction QL-3. Result It was found that five active fractions (QL-1, QL-3, QL-5, QL-7 and QL-11) were separated from Qinlian Siwu decoction, mainly contributed to the observed antagonismto the Contraction of the mouse Uterus. 28 compounds in the fraction QL-3 were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, chuanxiongzine hydrochloridum, vanillic acid, caffeic acid, paeoniflorin, berberastine, albiflorin, tetrahydropalmatine, coptisine, jatrorrhizine, leonuride, worenine, ferulic acid, palmatine, berberine, scutellarin, baicalin-7-0-glucoside, baicalin, rehmannioside C, wogonoside, chrysin-7-glucuronide, ttetuin, baicalein, wogonin and oroxylin-A. Conclusion In vitro inhibiting the Contraction of the isolated mouse uterine of Qinlian Siwu decoction was mainly attributed to the fraction QL-1 and QL-3. The active fractions (QL-5, QL-7 and QL-11) were obtained from QL-3 on the macroporous adsorptive resins by the gradient elution using ethanol.
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Inhibitory effects of active fraction and its main components of Shaofu Zhuyu decoction on Uterus Contraction.
The American journal of Chinese medicine, 2010Co-Authors: Yongqing Hua, Jin-ao Duan, Erxin Shang, Wei Zhou, Yuping TangAbstract:Shaofu Zhuyu decoction is a famous formula for treating primary dysmenorrhea in China since the Qing dynasty. In this paper, the inhibitory effects of active-guided fraction and its main bioactive components of Shaofu Zhuyu decoction on a model of non-pregnant mice uterine Contraction induced by oxytocin in vitro were investigated. Qualitative and quantitative chemical analyses were used to correlate the chemical composition of active fraction with the spasmolytic effects. Seven ingredients in the active fraction were identified and quantified by HPLC-DAD. Three ingredients, ferulic acid, vanillic acid, and typhaneoside, were evaluated for their effects on mice isolated uterine Contraction induced by oxytocin in vitro. The ED(50) of them were 63.0 microg/ml, 57.6 microg/ml, 109.7 microg/ml, respectively. Furthermore, the inhibitory activity of the combination of these three compounds was prior to the fraction and seven compounds group. The ED(50) was 65.5 microg/ml. The data stated that ferulic acid, vanillic acid, and typhaneoside were possibly the main active components in the bioactive fraction of Shaofu Zhuyu decoction. The study also implied that Shaofu Zhuyu decoction may have direct inhibitory effects on the contractility of the mice Uterus and justified the traditional use of the prescription for treating the uterine cramping associated dysmenorrhea.
