The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
Anna Klukovits - One of the best experts on this subject based on the ideXlab platform.
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a nocisztatin es a nociceptin orphanin fq opioid szerű peptidek molekularis szintű hatasainak vizsgalata a terhes meh műkodesere elucidation of the molecular mechanisms underlying the actions of opioid like peptides nocistatin and nociceptin orphanin
2013Co-Authors: Anna KlukovitsAbstract:A palyazat kereteben megvalosult vizsgalatok egy olyan osszetett munka reszet kepezik, melynek celja a terhes mehműkodest szabalyozo neuropeptid mediatorok szerepenek megismerese. 1. Igazoltuk, hogy a prepronociceptin (PNOC) mRNS-e expresszalodik a terhes patkany es human Uteruszban. Radioimmunoassay segitsegevel kimutattuk a nocisztatin (NST) feherje jelenletet a patkany Uteruszban. 2. In vitro izolalt szervi kiserletek soran detektaltuk, hogy a NST Uterusz relaxalo hatassal bir a terhes patkany Uteruszban. A relaxalo hatast az egyidejűleg adott nociceptin (N/OFQ) fokozta. 3. A NST onmagaban es N/OFQ jelenleteben is mersekelten fokozta a cAMP mennyiseget a terhes patkany Uteruszban. 4. A NST Uterusz relaxalo hatasat a Ca2+ -fuggő K+ csatornak nyitasa, a capsaicin-szenzitiv erző rostok aktivacioja altal kivaltott calcitonin gen-relacios peptid (CGRP) felszabadulas, es a szoveti G-feherjek aktivacioja kovetkezteben megemelkedett cAMP szint kozvetitik. 5. A NST Uterusz relaxalo hatast valtott ki in vitro izolalt szervrendszerben, normal terminusbol es koraszulesből szarmazo human mintakon. A relaxalo hatast az egyidejűleg adott N/OFQ nem befolyasolta. | The project was part of a systematic approach to explore the roles of neuropeptide modulators in uterine contractility. 1. The expression of prepronociceptin (PNOC) mRNA was verified in the pregnant rat and human myometrium. The presence of NST peptide in the rat Uterus was confirmed by radioimmunoassay. 2. In in vitro contractility assays, nocistatin (NST) inhibited uterine contractions in the intact rats at term. This effect was potentiated by nociceptin (N/OFQ). 3. NST alone and in combination with NOFQ induced a moderate cAMP accumulation in the intact rats’ Uterus at term. 4. NST elicited Uterus Relaxation via opening Ca2+ -dependent K+ channels, activating uterine sensory nerves and triggering calcitonin gene-related peptide (CGRP) release, and stimulating G-proteins and elevating uterine cAMP levels. 5. In in vitro contractility assays, NST inhibited uterine contractions in isolated human myometrial strips, collected from cesarean sections at term and from preterm labor, respectively. Co-administration of N/OFQ with NST increased the Uterus-relaxant effect of NST in uterine specimens from term but not preterm labour.
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inflammatory processes enhance camp mediated Uterus Relaxation in the pregnant rat the role of tnf α
Naunyn-schmiedebergs Archives of Pharmacology, 2009Co-Authors: Anna Klukovits, Arpad Marki, Eszter Paldy, Sandor Benyhe, Marta Galik, George Falkay, Robert GasparAbstract:The objective of this study was to assess the in vitro Uterus relaxing potency of β2-adrenergic receptor (β2-AR) agonists in pregnant rats after in utero administration of the bacterial lipopolysaccharide, Escherichia coli endotoxin (LPS). The LPS (100 μg/kg) was injected into the uterine lumen on day 16 of pregnancy. The effects of β2-AR agonist terbutaline was tested in vitro, in isolated uterine rings precontracted by electric field stimulation. Uterine β2-AR densities were detected by radioligand binding assay, the activated G-protein levels were investigated by a radiolabelled GTP binding assay. Uterine cAMP accumulation and the serum tumor necrosis factor-α (TNF-α) levels were measured by enzyme immunoassay. The endotoxin-evoked preterm delivery occurred on day 21. Higher pD2 values of terbutaline (p 0.05) in LPS-treated vs. control animals. Serum TNF-α level rose threefold after LPS treatment, but this rise was abolished by thalidomide. In LPS + thalidomide-treated rats, the effect of terbutaline became similar to that in sham-operated controls. By the measurement of myometrial cAMP levels, we documented that the concentration–response curve of terbutaline on cAMP accumulation was shifted to the left in the LPS-treated rats, with a significant rise in the pD2. We concluded that in the case of uterine inflammation, the in vitro Uterus-relaxing potency of β2-agonists enhances, which is possibly mediated by TNF-α and uterine cAMP levels and that may serve as a rationale for the use of β2-AR agonists in the attenuation of preterm uterine contractions on an inflammatory basis.
Stanley Zinberg - One of the best experts on this subject based on the ideXlab platform.
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intravenous nitroglycerin for uterine Relaxation of an inverted Uterus
American Journal of Obstetrics and Gynecology, 1992Co-Authors: Kenneth M Altabef, James T Spencer, Stanley ZinbergAbstract:Intravenous nitroglycerin has recently come to the attention of obstetricians as a potent uterine relaxant. In the case presented, it was used to permit replacement of a tightly contracted, inverted Uterus. Relaxation was rapid, effective, and associated with no adverse side effects.
Klukovits Anna - One of the best experts on this subject based on the ideXlab platform.
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A nocisztatin és a nociceptin / orphanin FQ opioid-szerű peptidek molekuláris szintű hatásainak vizsgálata a terhes méh működésére = Elucidation of the molecular mechanisms underlying the actions of opioid-like peptides nocistatin and nociceptin /
OTKA, 2013Co-Authors: Klukovits AnnaAbstract:A pályázat keretében megvalósult vizsgálatok egy olyan összetett munka részét képezik, melynek célja a terhes méhműködést szabályozó neuropeptid mediátorok szerepének megismerése. 1. Igazoltuk, hogy a prepronociceptin (PNOC) mRNS-e expresszálódik a terhes patkány és humán Uteruszban. Radioimmunoassay segítségével kimutattuk a nocisztatin (NST) fehérje jelenlétét a patkány Uteruszban. 2. In vitro izolált szervi kísérletek során detektáltuk, hogy a NST Uterusz relaxáló hatással bír a terhes patkány Uteruszban. A relaxáló hatást az egyidejűleg adott nociceptin (N/OFQ) fokozta. 3. A NST önmagában és N/OFQ jelenlétében is mérsékelten fokozta a cAMP mennyiségét a terhes patkány Uteruszban. 4. A NST Uterusz relaxáló hatását a Ca2+ -függő K+ csatornák nyitása, a capsaicin-szenzitív érző rostok aktivációja által kiváltott calcitonin gén-relációs peptid (CGRP) felszabadulás, és a szöveti G-fehérjék aktivációja következtében megemelkedett cAMP szint közvetítik. 5. A NST Uterusz relaxáló hatást váltott ki in vitro izolált szervrendszerben, normál terminusból és koraszülésből származó humán mintákon. A relaxáló hatást az egyidejűleg adott N/OFQ nem befolyásolta. | The project was part of a systematic approach to explore the roles of neuropeptide modulators in uterine contractility. 1. The expression of prepronociceptin (PNOC) mRNA was verified in the pregnant rat and human myometrium. The presence of NST peptide in the rat Uterus was confirmed by radioimmunoassay. 2. In in vitro contractility assays, nocistatin (NST) inhibited uterine contractions in the intact rats at term. This effect was potentiated by nociceptin (N/OFQ). 3. NST alone and in combination with NOFQ induced a moderate cAMP accumulation in the intact rats’ Uterus at term. 4. NST elicited Uterus Relaxation via opening Ca2+ -dependent K+ channels, activating uterine sensory nerves and triggering calcitonin gene-related peptide (CGRP) release, and stimulating G-proteins and elevating uterine cAMP levels. 5. In in vitro contractility assays, NST inhibited uterine contractions in isolated human myometrial strips, collected from cesarean sections at term and from preterm labor, respectively. Co-administration of N/OFQ with NST increased the Uterus-relaxant effect of NST in uterine specimens from term but not preterm labour
Robert Gaspar - One of the best experts on this subject based on the ideXlab platform.
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inflammatory processes enhance camp mediated Uterus Relaxation in the pregnant rat the role of tnf α
Naunyn-schmiedebergs Archives of Pharmacology, 2009Co-Authors: Anna Klukovits, Arpad Marki, Eszter Paldy, Sandor Benyhe, Marta Galik, George Falkay, Robert GasparAbstract:The objective of this study was to assess the in vitro Uterus relaxing potency of β2-adrenergic receptor (β2-AR) agonists in pregnant rats after in utero administration of the bacterial lipopolysaccharide, Escherichia coli endotoxin (LPS). The LPS (100 μg/kg) was injected into the uterine lumen on day 16 of pregnancy. The effects of β2-AR agonist terbutaline was tested in vitro, in isolated uterine rings precontracted by electric field stimulation. Uterine β2-AR densities were detected by radioligand binding assay, the activated G-protein levels were investigated by a radiolabelled GTP binding assay. Uterine cAMP accumulation and the serum tumor necrosis factor-α (TNF-α) levels were measured by enzyme immunoassay. The endotoxin-evoked preterm delivery occurred on day 21. Higher pD2 values of terbutaline (p 0.05) in LPS-treated vs. control animals. Serum TNF-α level rose threefold after LPS treatment, but this rise was abolished by thalidomide. In LPS + thalidomide-treated rats, the effect of terbutaline became similar to that in sham-operated controls. By the measurement of myometrial cAMP levels, we documented that the concentration–response curve of terbutaline on cAMP accumulation was shifted to the left in the LPS-treated rats, with a significant rise in the pD2. We concluded that in the case of uterine inflammation, the in vitro Uterus-relaxing potency of β2-agonists enhances, which is possibly mediated by TNF-α and uterine cAMP levels and that may serve as a rationale for the use of β2-AR agonists in the attenuation of preterm uterine contractions on an inflammatory basis.
Kenneth M Altabef - One of the best experts on this subject based on the ideXlab platform.
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intravenous nitroglycerin for uterine Relaxation of an inverted Uterus
American Journal of Obstetrics and Gynecology, 1992Co-Authors: Kenneth M Altabef, James T Spencer, Stanley ZinbergAbstract:Intravenous nitroglycerin has recently come to the attention of obstetricians as a potent uterine relaxant. In the case presented, it was used to permit replacement of a tightly contracted, inverted Uterus. Relaxation was rapid, effective, and associated with no adverse side effects.
