Vaccinium corymbosum

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 2760 Experts worldwide ranked by ideXlab platform

Marjorie Reyesdiaz - One of the best experts on this subject based on the ideXlab platform.

  • different levels of uv b resistance in Vaccinium corymbosum cultivars reveal distinct backgrounds of phenylpropanoid metabolites
    Plant Physiology and Biochemistry, 2017
    Co-Authors: Ana Luengo Escobar, Miren Alberdi, Adriano Nunesnesi, Franklin Magnum De Oliveira Silva, Patricio Acevedo, Marjorie Reyesdiaz
    Abstract:

    UV-B radiation induces several physiological and biochemical effects that can influence regulatory plant processes. Vaccinium corymbosum responds differently to UV-B radiation depending on the UV-B resistance of cultivars, according to their physiological and biochemical features. In this work, the effect of two levels of UV-B radiation during long-term exposure on the phenylpropanoid biosynthesis, and the expression of genes associated with flavonoid biosynthesis as well as the absolute quantification of secondary metabolites were studied in two contrasting UV-B-resistant cultivars (Legacy, resistant and Bluegold, sensitive). Multivariate analyses were performed to understand the role of phenylpropanoids in UV-B defense mechanisms. The amount of phenylpropanoid compounds was generally higher in Legacy than in Bluegold. Different expression levels of flavonoid biosynthetic genes for both cultivars were transiently induced, showing that even in longer period of UV-B exposure; plants are still adjusting their phenylpropanoids at the transcription levels. Multivariate analysis in Legacy indicated no significant correlation between gene expression and the levels of the flavonoids and phenolic acids. By contrast, in the Bluegold cultivar higher number of correlations between secondary metabolite and transcript levels was found. Taken together, the results indicated different adjustments between the cultivars for a successful UV-B acclimation. While the sensitive cultivar depends on metabolite adjustments to respond to UV-B exposure, the resistant cultivar also possesses an intrinsically higher antioxidant and UV-B screening capacity. Thus, we conclude that UV-B resistance involves not only metabolite level adjustments during the acclimation period, but also depends on the intrinsic metabolic status of the plant and metabolic features of the phenylpropanoid compounds.

  • the effect of foliar boron spraying on the fruit features of brigitta and legacy highbush blueberry Vaccinium corymbosum cultivars
    Ciencia E Investigacion Agraria, 2016
    Co-Authors: Cristian Merinogergichevich, Eduardo Pacheco, Marjorie Reyesdiaz
    Abstract:

    Boron (B) is associated with reproductive and antioxidant metabolism in plants, and in southern Chile, the deficiency of this micronutrient is ameliorated through foliar applications that are a part of agricultural management, thus ensuring the productivity of crops including highbush blueberry ( Vaccinium corymbosum L.). However, the appropriate B dosage for this species is unknown, so the aim of this study was to evaluate foliar B application on fruit quality in highbush blueberry in southern Chile. During the 2014-2015 season, Brigitta and Legacy cultivars were sprayed with B at rates of 0 (control), 200, 400 and 800 mg L-1 three times (10% bloom, full bloom, and fruit set), and fruit set (%), firmness (g mm-2), soluble solids (°Brix), weight (g) and size (mm) of the fruits were determined. In addition, antioxidant activity was measured by ORAC (oxygen radical absorbance capacity) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The results showed that a low rate of B (200 mg L-1) increased the fruit set and soluble solids in Brigitta, whereas Legacy required higher B concentrations (400 and 800 mg L-1). In both cultivars, firmness, fresh fruit weight, dry matter (%) and antioxidant activity increased under the 200 and 400 mg B L-1 treatments, whereas this last parameter was reduced under a high B concentration. A low B rate showed to be more effective at improving fruit quality in Brigitta, while Legacy required higher B application rates. Future research should be performed over several seasons and different cultivars to confirm these results. El boro (B) esta asociado al metabolismo reproductivo y antioxidante en plantas. En el sur de Chile, su deficiencia es resuelta con aplicaciones foliares de este micronutriente como parte del manejo agronomico, asegurando asi la productividad en cultivos como arandano alto ( Vaccinium corymbosum L.). Sin embargo, la informacion sobre dosis adecuadas para esta especie es escasa. Se evaluo la aplicacion foliar de B sobre parametros de calidad en fruta de arandano alto del sur de Chile. En la temporada 2014-2015, plantas de cultivares Brigitta y Legacy, fueron asperjadas con B en dosis de 0 (control), 200, 400 y 800 mg L-1 en tres momentos durante la temporada (10% floracion, plena flor y fruto cuajado), determinandose: frutos cuajados (%), firmeza (g mm-2), solidos solubles (oBrix), peso (g) y calibre (mm), ademas de la actividad antioxidante mediante ORAC (oxygen radical absorbance capacity) y DPPH (2,2-diphenyl-1-picrylhydrazyl). Una baja dosis de B (200 mg L-1) incremento la cuaja y solidos solubles en Brigitta, mientras que Legacy requirio mayores concentraciones de B (400 y 800 mg L-1). En ambos cultivares aumento la firmeza, peso fresco y seco de la fruta, asi como la actividad antioxidante al ser tratada con 200 y 400 mg B L-1, mientras en alta concentracion disminuyo este ultimo parametro. Una baja dosis de B resulto ser efectiva mejorando la calidad de la fruta en el cultivar Brigitta, mientras que Legacy requirio dosis de B mas alta. Futuras investigaciones deberian ser realizadas en diferentes temporadas y cultivares para confirmar esta tendencia.

  • rearrangement of leaf traits with changing source sink relationship in blueberry Vaccinium corymbosum l leaves
    Photosynthetica, 2016
    Co-Authors: Emilio Jorquerafontena, Marjorie Reyesdiaz, Miren Alberdi, N. Franck
    Abstract:

    The source-sink relationship is one of major determinants of plant performance. The influence of reproductive sink demand on light-saturated photosynthesis (P max), dark respiration (R D), stomatal conductance (g s), intrinsic water-use efficiency (WUEi), contents of soluble sugar (SSC), nitrogen, carbon, and photosynthetic pigments was examined in blueberry (Vaccinium corymbosum L. cv. ‘Brigitta’) during the final stage of rapid fruit growth. Measurements were performed three times per day on developed, sun-exposed leaves of girdled shoots with 0.1, 1, and 10 fruit per leaf (0.1F:L, 1F:L, and 10F:L, respectively) and nongirdled shoots bearing one fruit per leaf (NG). Girdling and lower fruit amount induced lower P max, g s, N, and total chlorophyll (Chl) and higher WUEi, SSC, R D, Chl a/b ratio and carotenoids-to-chlorophylls ratio (Car/Chl) for the 1F:L and 0.1F:L treatments. The impact of girdling was counterbalanced by 10F:L, with NG and 10F:L having similar values. Variables other than P max, R D, g s, WUEi, and SSC were unaffected throughout the course of the day. P max and g s decreased during the course of the day, but g s decreased more than P max in the afternoon, while WUEi was increasing in almost all treatments. SSC increased from the morning until afternoon, whereas R D peaked at noon regardless of the treatment. Generally, P max was closely and negatively correlated to SSC, indicating that sugar-sensing mechanisms played an important role in regulation of blueberry leaf photosynthesis. With respect to treatments, P max and N content were positively related, while R D was not associated to substrate availability. The enhanced Car/Chl ratio showed a higher photoprotection under the lower sink demand. Changes in the source-sink relationship in ‘Brigitta’ blueberry led to a rearrangement of physiological and structural leaf traits which allowed adjusting the daily balance between carbon assimilation and absorbed light energy.

  • leaf nitrogen thresholds ensuring high antioxidant features of Vaccinium corymbosum cultivars
    Journal of Soil Science and Plant Nutrition, 2015
    Co-Authors: Erwin Yanezmansilla, Marjorie Reyesdiaz, Paula Cartes, Alejandra Riberafonseca, Zed Rengel, Miren Alberdi
    Abstract:

    Deficiency or excess of nitrogen (N) supply can promote formation of reactive oxygen species in plants, inducing oxidative stress. Otherwise, plants may enhance phenolics biosynthesis and antioxidant capacity under N deficiency, but this effect is plant species-dependent. There is no information about influence on phenolics and antioxidant activity in highbush blueberry (Vaccinium corymbosum L.), in which quality and commercial importance depend on high phenolics concentration. We studied the effect of variable N supply (0 to 38 mM) on N uptake and antioxidant responses in two highbush blueberry cultivars (Legacy and Bluegold) hydroponically grown at the long-term. Nitrogen leaves concentration was enhanced for both cultivars at increasing N supply. Bluegold decreased CO2 assimilation at 0 N treatment, possibly due to both, insufficient N concentration and a decline in superoxide dismutase (SOD) activity. In contrast, SOD was activated in Legacy at 0 N, and interestingly only this cultivar maintained CO2 assimilation rates across all N treatments. Both cultivars showed higher phenolics and antioxidant activity levels at 9 mM. Despite the differential responses among the cultivars, we propose a threshold of 15 g N kg-1 DW to ensure high antioxidant activity and quality in blueberry leaves.

  • manganese toxicity and uv b radiation differentially influence the physiology and biochemistry of highbush blueberry Vaccinium corymbosum cultivars
    Functional Plant Biology, 2014
    Co-Authors: Yesenia Rojaslillo, Miren Alberdi, Zed Rengel, Claudio Inostrozablancheteau, María Luz Mora, Patricio Acevedo, Marjorie Reyesdiaz
    Abstract:

    Manganese (Mn2+) toxicity or UV-B radiation and their individual effects on plants have been documented previously. However, no study about the combined effect of these stresses is available. We evaluated the individual and combined effects of excess Mn2+ and UV-B radiation on physiological and biochemical parameters in two highbush blueberry (Vaccinium corymbosum L.) cultivars differing in resistance to Mn toxicity (Brigitta (resistant) and Bluegold (sensitive)). Plants grown in Hoagland nutrient solution were subjected to the following treatments: 2µM MnCl2 (control), 500µM MnCl2 (toxic Mn2+), UV-B radiation (a daily dose of 94.4kJm-2), and the combined treatment (toxic Mn2++UV-B) for 30 days. In both cultivars, the Mn2++UV-B treatment caused a more negative effect on net photosynthesis (Pn), stomatal conductance (gs), the photochemical parameters of PSII and the chl a/b ratio than the treatments with toxic Mn2+ or UV-B alone. However, Brigitta showed also a better acclimation response in Pn and gs than Bluegold at the end of the experiment. The Mn2++UV-B treatment inhibited growth, enhanced radical scavenging activity and superoxide dismutase activity, and increased the concentration of total UV-absorbing compounds, phenols and anthocyanins, mainly in Bluegold. In conclusion, Mn-resistant Brigitta showed a better acclimation response and greater resistance to the combined stress of Mn2+ toxicity and UV-B exposure than the Mn-sensitive Bluegold. An increased concentration of photoprotective compounds and enhanced resistance to oxidative stress in Brigitta could underpin increased resistance to the combined stress.

Meza Davey Bárbara - One of the best experts on this subject based on the ideXlab platform.

Edgardo Cruces - One of the best experts on this subject based on the ideXlab platform.

  • interactive effects of aluminum and cadmium on phenolic compounds antioxidant enzyme activity and oxidative stress in blueberry Vaccinium corymbosum l plantlets cultivated in vitro
    Ecotoxicology and Environmental Safety, 2018
    Co-Authors: Karen Manquiancerda, Gustavo E Zuniga, Mauricio Escudey, Edgardo Cruces, R Calderon
    Abstract:

    To evaluate the potential role of phenolic compounds in Al and Cd stress tolerance mechanisms, Vaccinium corymbosum cv. Legacy plantlets were exposed to different metal concentrations. The present study used an in vitro plant model to test the effects of the following treatments: 100μM Al; 100μMAl + 50μMCd; and 100μMAl + 100μMCd during periods of 7, 14, 21 and 30 days. The oxidative damage was determined by the accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2). The antioxidant activity values were determined using 1,1-diphenyl-2-picrylhydrazine (DPPH) and the ferric reducing antioxidant power test (FRAP). Additionally, the phenolic compound concentrations were determined using HPLC-DAD. The exposure to Al and Cd increased the MDA and H2O2 contents differentially, while the antioxidant capacity values showed differences between DPPH and FRAP with the largest changes in FRAP relative to Cd. SOD had the highest activity in the first 7 days, leading to a significant increase in phenolic compounds observed after 14 days, and chlorogenic acid was the major compound identified. Our results revealed that phenolic compounds seem to play an important role in the response to ROS. Therefore, the mechanisms of tolerance to Al and Cd in V. corymbosum will be determined by the type of metal and time of exposure.

  • effect of cadmium on phenolic compounds antioxidant enzyme activity and oxidative stress in blueberry Vaccinium corymbosum l plantlets grown in vitro
    Ecotoxicology and Environmental Safety, 2016
    Co-Authors: Karen Manquiancerda, Gustavo E Zuniga, Mauricio Escudey, Nicolas Arancibiamiranda, Mauricio Molina, Edgardo Cruces
    Abstract:

    Abstract Cadmium (Cd 2+ ) can affect plant growth due to its mobility and toxicity. We evaluated the effects of Cd 2+ on the production of phenolic compounds and antioxidant response of Vaccinium corymbosum L. Plantlets were exposed to Cd 2+ at 50 and 100 µM for 7, 14 and 21 days. Accumulation of malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) and the antioxidant enzyme SOD was determined. The profile of phenolic compounds was evaluated using LC-MS. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power test (FRAP). Cd 2+ increased the content of MDA, with the highest increase at 14 days. The presence of Cd 2+ resulted in changes in phenolic compounds. The main phenolic compound found in blueberry plantlets was chlorogenic acid, whose abundance increased with the addition of Cd 2+ to the medium. The changes in the composition of phenolic compounds showed a positive correlation with the antioxidant activity measured using FRAP. Our results suggest that blueberry plantlets produced phenolic compounds with reducing capacity as a selective mechanism triggered by the highest activity of Cd 2+ .

Karen Manquiancerda - One of the best experts on this subject based on the ideXlab platform.

  • interactive effects of aluminum and cadmium on phenolic compounds antioxidant enzyme activity and oxidative stress in blueberry Vaccinium corymbosum l plantlets cultivated in vitro
    Ecotoxicology and Environmental Safety, 2018
    Co-Authors: Karen Manquiancerda, Gustavo E Zuniga, Mauricio Escudey, Edgardo Cruces, R Calderon
    Abstract:

    To evaluate the potential role of phenolic compounds in Al and Cd stress tolerance mechanisms, Vaccinium corymbosum cv. Legacy plantlets were exposed to different metal concentrations. The present study used an in vitro plant model to test the effects of the following treatments: 100μM Al; 100μMAl + 50μMCd; and 100μMAl + 100μMCd during periods of 7, 14, 21 and 30 days. The oxidative damage was determined by the accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2). The antioxidant activity values were determined using 1,1-diphenyl-2-picrylhydrazine (DPPH) and the ferric reducing antioxidant power test (FRAP). Additionally, the phenolic compound concentrations were determined using HPLC-DAD. The exposure to Al and Cd increased the MDA and H2O2 contents differentially, while the antioxidant capacity values showed differences between DPPH and FRAP with the largest changes in FRAP relative to Cd. SOD had the highest activity in the first 7 days, leading to a significant increase in phenolic compounds observed after 14 days, and chlorogenic acid was the major compound identified. Our results revealed that phenolic compounds seem to play an important role in the response to ROS. Therefore, the mechanisms of tolerance to Al and Cd in V. corymbosum will be determined by the type of metal and time of exposure.

  • effect of cadmium on phenolic compounds antioxidant enzyme activity and oxidative stress in blueberry Vaccinium corymbosum l plantlets grown in vitro
    Ecotoxicology and Environmental Safety, 2016
    Co-Authors: Karen Manquiancerda, Gustavo E Zuniga, Mauricio Escudey, Nicolas Arancibiamiranda, Mauricio Molina, Edgardo Cruces
    Abstract:

    Abstract Cadmium (Cd 2+ ) can affect plant growth due to its mobility and toxicity. We evaluated the effects of Cd 2+ on the production of phenolic compounds and antioxidant response of Vaccinium corymbosum L. Plantlets were exposed to Cd 2+ at 50 and 100 µM for 7, 14 and 21 days. Accumulation of malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) and the antioxidant enzyme SOD was determined. The profile of phenolic compounds was evaluated using LC-MS. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power test (FRAP). Cd 2+ increased the content of MDA, with the highest increase at 14 days. The presence of Cd 2+ resulted in changes in phenolic compounds. The main phenolic compound found in blueberry plantlets was chlorogenic acid, whose abundance increased with the addition of Cd 2+ to the medium. The changes in the composition of phenolic compounds showed a positive correlation with the antioxidant activity measured using FRAP. Our results suggest that blueberry plantlets produced phenolic compounds with reducing capacity as a selective mechanism triggered by the highest activity of Cd 2+ .

Price Passalacqua, Daniella Selma - One of the best experts on this subject based on the ideXlab platform.