The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
Igić Rajko - One of the best experts on this subject based on the ideXlab platform.
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An Advice for Young Researchers
NULRS, 2018Co-Authors: Igić RajkoAbstract:For a young researcher, the best way to improve his skills and develop his research capabilities is to work in established research laboratories where he is enabled to learn modern techniques and how to attack the scientific problems. Today, we have easy communications, including computers and the internet, but direct interactions with the most experienced scientists are the best way for young scientist to advance his research capabilities. Ulf Svante von Euler, Swedish pharmacologist and physiologist presents the best example that illustrates how interaction of a young researcher with established scientists develop his research capabilities and become a well-known scientist1.When Ulf was seventeen (1922), he came in Stockholm to study medicine. As a student, he became interested in research, and in 1926 he attended the Twelfth International Congress of Physiologists in Stockholm where he heard lectures by I. P. Pavlov, E. H. Starling and other great scientists of the time. He also observed a historic demonstration by Otto Loewi on the existence of Vagusstoff in the frog’s heart, which would stimulate his own interest and research on mediators of nerve transmission. Prior to this demonstration, Loewi had published several papers on the nature of this chemical substance that slowed the heart, but not all of his research contemporaries were convinced. However, a successful demonstration at the Congress (repeated eighteen times) convinced all critics. Von Euler recalled that these experiments inspired his enduring interest in neurohumoral transmission.Initially, von Euler was influenced by several well-known Swedish scientists: G. Liljestrand (pharmacologist/physiologist), R. Fåraeus (a hematologist) and H. Theorell (a biochemist, who received the Nobel Prize for Medicine and Physiology in 1955). Ulf defended his doctoral dissertation in 1930 and became a professor of pharmacology. Then, he received a two-year scholarship for postdoctoral studies abroad that enabled him to improve his skills by working with several famous foreign researchers.The young Ulf von Euler made the most of this opportunity. He spent six months in Hampstead at Sir Henry Dale’s laboratory, two months in Birmingham with I. de Burgh Daly, eight months in Ghent with C. Heymans, and three months in Frankfurt with G. Embden. Later, in 1934, he returned to London for six months to work with A. Hill, primarily because Liljestrand advised him instead of pharmacology, rather to devote to physiology because at that time in Sweden this scientific discipline was more appreciated. Towards the end of 1937, he went back to Hampstead for five months to work again with Sir Henry Dale
Dirk Trauner - One of the best experts on this subject based on the ideXlab platform.
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Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors
2012Co-Authors: Ivan Tochitsky, Matthew R. Banghart, Re Mourot, Jennifer Z. Yao, Benjamin Gaub, Richard H. Kramer, Dirk TraunerAbstract:Advances in synthetic chemistry, structural biology, molecular modelling and molecular cloning have enabled the systematic functional manipulation of transmembrane proteins. By combining genetically manipulated proteins with light-sensitive ligands, innately ‘blind ’ neurobiological receptors can be converted into photoreceptors, which allows them to be photoregulated with high spatiotemporal precision. Here, we present the optochemical control of neuronal nicotinic acetylcholine receptors (nAChRs) with photoswitchable tethered agonists and antagonists. Using structure-based design, we produced heteromeric a3b4 and a4b2 nAChRs that can be activated or inhibited with deep-violet light, but respond normally to acetylcholine in the dark. The generation of these engineered receptors should facilitate investigation of the physiological and pathological functions of neuronal nAChRs and open a general pathway to photosensitizing pentameric ligand-gated ion channels. A cetylcholine (ACh) and its receptors have always been at the forefront of new developments in physiology. With its iso-lation as ‘Vagusstoff ’ in 1921, Otto Loewi established that a small diffusible molecule could mediate nervous activity and shaped the concept of a neurotransmitter1. Nicotinic acetylcholin
Ivan Tochitsky - One of the best experts on this subject based on the ideXlab platform.
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Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors
2012Co-Authors: Ivan Tochitsky, Matthew R. Banghart, Re Mourot, Jennifer Z. Yao, Benjamin Gaub, Richard H. Kramer, Dirk TraunerAbstract:Advances in synthetic chemistry, structural biology, molecular modelling and molecular cloning have enabled the systematic functional manipulation of transmembrane proteins. By combining genetically manipulated proteins with light-sensitive ligands, innately ‘blind ’ neurobiological receptors can be converted into photoreceptors, which allows them to be photoregulated with high spatiotemporal precision. Here, we present the optochemical control of neuronal nicotinic acetylcholine receptors (nAChRs) with photoswitchable tethered agonists and antagonists. Using structure-based design, we produced heteromeric a3b4 and a4b2 nAChRs that can be activated or inhibited with deep-violet light, but respond normally to acetylcholine in the dark. The generation of these engineered receptors should facilitate investigation of the physiological and pathological functions of neuronal nAChRs and open a general pathway to photosensitizing pentameric ligand-gated ion channels. A cetylcholine (ACh) and its receptors have always been at the forefront of new developments in physiology. With its iso-lation as ‘Vagusstoff ’ in 1921, Otto Loewi established that a small diffusible molecule could mediate nervous activity and shaped the concept of a neurotransmitter1. Nicotinic acetylcholin
Matthew R. Banghart - One of the best experts on this subject based on the ideXlab platform.
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Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors
2012Co-Authors: Ivan Tochitsky, Matthew R. Banghart, Re Mourot, Jennifer Z. Yao, Benjamin Gaub, Richard H. Kramer, Dirk TraunerAbstract:Advances in synthetic chemistry, structural biology, molecular modelling and molecular cloning have enabled the systematic functional manipulation of transmembrane proteins. By combining genetically manipulated proteins with light-sensitive ligands, innately ‘blind ’ neurobiological receptors can be converted into photoreceptors, which allows them to be photoregulated with high spatiotemporal precision. Here, we present the optochemical control of neuronal nicotinic acetylcholine receptors (nAChRs) with photoswitchable tethered agonists and antagonists. Using structure-based design, we produced heteromeric a3b4 and a4b2 nAChRs that can be activated or inhibited with deep-violet light, but respond normally to acetylcholine in the dark. The generation of these engineered receptors should facilitate investigation of the physiological and pathological functions of neuronal nAChRs and open a general pathway to photosensitizing pentameric ligand-gated ion channels. A cetylcholine (ACh) and its receptors have always been at the forefront of new developments in physiology. With its iso-lation as ‘Vagusstoff ’ in 1921, Otto Loewi established that a small diffusible molecule could mediate nervous activity and shaped the concept of a neurotransmitter1. Nicotinic acetylcholin
Re Mourot - One of the best experts on this subject based on the ideXlab platform.
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Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors
2012Co-Authors: Ivan Tochitsky, Matthew R. Banghart, Re Mourot, Jennifer Z. Yao, Benjamin Gaub, Richard H. Kramer, Dirk TraunerAbstract:Advances in synthetic chemistry, structural biology, molecular modelling and molecular cloning have enabled the systematic functional manipulation of transmembrane proteins. By combining genetically manipulated proteins with light-sensitive ligands, innately ‘blind ’ neurobiological receptors can be converted into photoreceptors, which allows them to be photoregulated with high spatiotemporal precision. Here, we present the optochemical control of neuronal nicotinic acetylcholine receptors (nAChRs) with photoswitchable tethered agonists and antagonists. Using structure-based design, we produced heteromeric a3b4 and a4b2 nAChRs that can be activated or inhibited with deep-violet light, but respond normally to acetylcholine in the dark. The generation of these engineered receptors should facilitate investigation of the physiological and pathological functions of neuronal nAChRs and open a general pathway to photosensitizing pentameric ligand-gated ion channels. A cetylcholine (ACh) and its receptors have always been at the forefront of new developments in physiology. With its iso-lation as ‘Vagusstoff ’ in 1921, Otto Loewi established that a small diffusible molecule could mediate nervous activity and shaped the concept of a neurotransmitter1. Nicotinic acetylcholin
