The Experts below are selected from a list of 159 Experts worldwide ranked by ideXlab platform
Robert K Winn - One of the best experts on this subject based on the ideXlab platform.
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sialyl lewis x oligosaccharide reduces ischemia reperfusion injury in the rabbit ear
Journal of Immunology, 1995Co-Authors: S R Sharar, M L Phillips, John M Harlan, Robert K WinnAbstract:Ischemia-reperfusion injury in the rabbit ear is neutrophil (PMN)-mediated, and is significantly reduced by anti-adhesion agents directed against beta 2 integrins, P-selectin, or L-selectin. We further examined selectin-mediated adherence in this setting following the administration of soluble sialyl Lewis(x) (SLe(x)), the principal carbohydrate ligand for P-, L-, and E-selectin, at various times following reperfusion. Under constant ambient temperature conditions, the rabbit ear Vascular supply was isolated and occluded with an atraumatic Vascular Clamp for 6 h, then allowed to reperfuse. Animals receiving i.v. SLe(x) (25 mg/kg bolus + 50 mg/kg infusion over 10 h) 1) at the time of reperfusion, 2) 1 h after reperfusion, 3) 4 h after reperfusion, or 4) 12 h after reperfusion were compared with control animals receiving either saline or sialyl lactosamine, an oligosaccharide structurally similar to SLe(x) but not involved in selectin recognition. Tissue injury was assessed by serial measurement of ear edema and by visual determination of ear necrosis over 7 days. Tissue edema and necrosis were significantly reduced in animals treated with SLe(x) immediately upon reperfusion or after a 1-h delay, but not in animals for whom SLe(x) administration was delayed by 4 or 12 h. Furthermore, SLe(x) administration alone had no effect on circulating leukocyte or PMN counts, or PMN expression of CD18 or L-selectin. We conclude that interruption of selectin-mediated adherence with soluble SLe(x) oligosaccharide attenuates reperfusion in the rabbit ear. The observation that SLe(x) is efficacious only if administered in the first hour after reperfusion suggests that the more immediately available P- and L-selectin participate in this PMN adhesion/injury process, whereas E-selectin, with its delayed endothelial expression, does not.
Koray Tekin - One of the best experts on this subject based on the ideXlab platform.
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Post-kidney transplantation external iliac artery stenosis due to Vascular Clamp: report of a case
Jornal Vascular Brasileiro, 2014Co-Authors: Murat Özban, Cagatay Aydin, Belda Dursun, Baki Yagci, Onur Birsen, Koray TekinAbstract:We report a case of right external iliac artery stenosis after kidney transplantation surgery caused by Vascular Clamp application injury. The patient presented with claudication of the ipsilateral lower limb and the lesion was diagnosed angiographically. The patient was treated with endoVascular stent placement.
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post kidney transplantation external iliac artery stenosis due to Vascular Clamp report of a case estenose de arteria iliaca externa apos transplante renal devido a Clampe Vascular relato de um caso
2014Co-Authors: Murat Özban, Cagatay Aydin, Belda Dursun, Baki Yagci, Onur Birsen, Koray TekinAbstract:We report a case of right external iliac artery stenosis after kidney transplantation surgery caused by Vascular Clamp application injury. The patient presented with claudication of the ipsilateral lower limb and the lesion was diagnosed angiographically. The patient was treated with endoVascular stent placement.
Kareem Abuelmagd - One of the best experts on this subject based on the ideXlab platform.
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immunologic challenges in small bowel transplantation
American Journal of Transplantation, 2012Co-Authors: Melvin Berger, A Zeevi, Douglas G Farmer, Kareem AbuelmagdAbstract:Since the introduction of tacrolimus, small-bowel and multivisceral transplantion has increased to 100–200/year in the United States. The intestine carries more passenger lymphocytes than other organs, and bidirectional trafficking of lymphocytes and other immunocytes begins as soon as the Vascular Clamp is released. Because of ischemia-reperfusion injury and exposure to ligands for Toll-like receptors from the lumen, the innate immune system of the graft is activated, causing inflammation which must be brought under control by regulatory cells. Inclusion of the liver in the allograft favors graft acceptance, but the mechanism of this effect has not been determined. Anti-HLA and other anti-donor antibodies clearly play a major role in determining the long-term fate of the graft, as reflected in 5-year graft survival. Development of new (de novo) HLA antibodies and/or increases in their titers or function—especially the ability to bind C1q and activate complement increase the risk of graft loss. Monitoring antidonor antibody production and the use of new therapies including complement inhibitors will contribute to increasing success of SBT.
S R Sharar - One of the best experts on this subject based on the ideXlab platform.
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sialyl lewis x oligosaccharide reduces ischemia reperfusion injury in the rabbit ear
Journal of Immunology, 1995Co-Authors: S R Sharar, M L Phillips, John M Harlan, Robert K WinnAbstract:Ischemia-reperfusion injury in the rabbit ear is neutrophil (PMN)-mediated, and is significantly reduced by anti-adhesion agents directed against beta 2 integrins, P-selectin, or L-selectin. We further examined selectin-mediated adherence in this setting following the administration of soluble sialyl Lewis(x) (SLe(x)), the principal carbohydrate ligand for P-, L-, and E-selectin, at various times following reperfusion. Under constant ambient temperature conditions, the rabbit ear Vascular supply was isolated and occluded with an atraumatic Vascular Clamp for 6 h, then allowed to reperfuse. Animals receiving i.v. SLe(x) (25 mg/kg bolus + 50 mg/kg infusion over 10 h) 1) at the time of reperfusion, 2) 1 h after reperfusion, 3) 4 h after reperfusion, or 4) 12 h after reperfusion were compared with control animals receiving either saline or sialyl lactosamine, an oligosaccharide structurally similar to SLe(x) but not involved in selectin recognition. Tissue injury was assessed by serial measurement of ear edema and by visual determination of ear necrosis over 7 days. Tissue edema and necrosis were significantly reduced in animals treated with SLe(x) immediately upon reperfusion or after a 1-h delay, but not in animals for whom SLe(x) administration was delayed by 4 or 12 h. Furthermore, SLe(x) administration alone had no effect on circulating leukocyte or PMN counts, or PMN expression of CD18 or L-selectin. We conclude that interruption of selectin-mediated adherence with soluble SLe(x) oligosaccharide attenuates reperfusion in the rabbit ear. The observation that SLe(x) is efficacious only if administered in the first hour after reperfusion suggests that the more immediately available P- and L-selectin participate in this PMN adhesion/injury process, whereas E-selectin, with its delayed endothelial expression, does not.
Murat Özban - One of the best experts on this subject based on the ideXlab platform.
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Post-kidney transplantation external iliac artery stenosis due to Vascular Clamp: report of a case
Jornal Vascular Brasileiro, 2014Co-Authors: Murat Özban, Cagatay Aydin, Belda Dursun, Baki Yagci, Onur Birsen, Koray TekinAbstract:We report a case of right external iliac artery stenosis after kidney transplantation surgery caused by Vascular Clamp application injury. The patient presented with claudication of the ipsilateral lower limb and the lesion was diagnosed angiographically. The patient was treated with endoVascular stent placement.
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post kidney transplantation external iliac artery stenosis due to Vascular Clamp report of a case estenose de arteria iliaca externa apos transplante renal devido a Clampe Vascular relato de um caso
2014Co-Authors: Murat Özban, Cagatay Aydin, Belda Dursun, Baki Yagci, Onur Birsen, Koray TekinAbstract:We report a case of right external iliac artery stenosis after kidney transplantation surgery caused by Vascular Clamp application injury. The patient presented with claudication of the ipsilateral lower limb and the lesion was diagnosed angiographically. The patient was treated with endoVascular stent placement.
