The Experts below are selected from a list of 240 Experts worldwide ranked by ideXlab platform
Wayne L Nicholson - One of the best experts on this subject based on the ideXlab platform.
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inactivation of Vegetative Cells but not spores of bacillus anthracis b cereus and b subtilis on stainless steel surfaces coated with an antimicrobial silver and zinc containing zeolite formulation
Applied and Environmental Microbiology, 2003Co-Authors: Belinda Galeano, Emily Korff, Wayne L NicholsonAbstract:Stainless steel surfaces coated with paints containing a silver- and zinc-containing zeolite (AgION antimicrobial) were assayed in comparison to uncoated stainless steel for antimicrobial activity against Vegetative Cells and spores of three Bacillus species, namely, B. anthracis Sterne, B. cereus T, and B. subtilis 168. Under the test conditions (25°C and 80% relative humidity), the zeolite coating produced approximately 3 log10 inactivation of Vegetative Cells within a 5- to 24-h period, but viability of spores of the three species was not significantly affected.
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the spectrum of spontaneous rifampin resistance mutations in the rpob gene of bacillus subtilis 168 spores differs from that of Vegetative Cells and resembles that of mycobacterium tuberculosis
Journal of Bacteriology, 2002Co-Authors: Wayne L Nicholson, Heather MaughanAbstract:Mutations causing rifampin resistance in Vegetative Cells of Bacillus subtilis 168 have thus far been mapped to a rather restricted set of alterations at either Q469 or H482 within cluster I of the rpoB gene encoding the β subunit of RNA polymerase. In this study, we demonstrated that spores of B. subtilis 168 exhibit a spectrum of spontaneous rifampin resistance mutations distinct from that of Vegetative Cells. In addition to the rpoB mutations Q469K, Q469R, and H482Y previously characterized in Vegetative Cells, we isolated a new mutation of rpoB, H482R, from Vegetative Cells. Additional new rifampin resistance mutations arising from spores were detected at A478N and most frequently at S487L. The S487L change is the predominant change found in rpoB mutations sequenced from rifampin-resistant clinical isolates of Mycobacterium tuberculosis. The observations are discussed in terms of the underlying differences of the DNA environment within dormant Cells and Vegetatively growing Cells.
Yenpeng Ting - One of the best experts on this subject based on the ideXlab platform.
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adhesion of b subtilis spores and Vegetative Cells onto stainless steel dlvo theories and afm spectroscopy
Journal of Colloid and Interface Science, 2013Co-Authors: Ardiyan Harimawan, Shaoping Zhong, Yenpeng TingAbstract:Abstract Interactions between the bacterium Bacillus subtilis (either as Vegetative Cells or as spores) and stainless steel 316 (SS-316) surfaces were quantified using the classical Derjaguin–Landau–Verwey–Overbeek (DLVO) theory and extended DLVO (xDLVO) approach in conjunction with live force spectroscopy using an Atomic Force Microscope (AFM). The xDLVO approach accounts for acid–base (polar) interactions that are not considered in the classical DLVO theory. AFM results revealed that spores manifested stronger attraction interactions to stainless steel compared to their Vegetative Cells counterparts due to lower energy barrier as predicted by both the theoretical approaches as well as the higher hydrophobicity on the spore surfaces. Both DLVO and xDLVO theories predict that Vegetative Cells manifest weaker attachment on the surfaces compared to spores. Results of AFM force measurement corroborate these findings; spores recorded significantly higher adhesion force (2.92 ± 0.4 nN) compared to Vegetative Cells (0.65 ± 0.2 nN). The adhesion of spores presents greater challenges in biofilm control owing to its stronger attachment and persistence when the spores are formed under adverse environmental conditions.
Ardiyan Harimawan - One of the best experts on this subject based on the ideXlab platform.
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adhesion of b subtilis spores and Vegetative Cells onto stainless steel dlvo theories and afm spectroscopy
Journal of Colloid and Interface Science, 2013Co-Authors: Ardiyan Harimawan, Shaoping Zhong, Yenpeng TingAbstract:Abstract Interactions between the bacterium Bacillus subtilis (either as Vegetative Cells or as spores) and stainless steel 316 (SS-316) surfaces were quantified using the classical Derjaguin–Landau–Verwey–Overbeek (DLVO) theory and extended DLVO (xDLVO) approach in conjunction with live force spectroscopy using an Atomic Force Microscope (AFM). The xDLVO approach accounts for acid–base (polar) interactions that are not considered in the classical DLVO theory. AFM results revealed that spores manifested stronger attraction interactions to stainless steel compared to their Vegetative Cells counterparts due to lower energy barrier as predicted by both the theoretical approaches as well as the higher hydrophobicity on the spore surfaces. Both DLVO and xDLVO theories predict that Vegetative Cells manifest weaker attachment on the surfaces compared to spores. Results of AFM force measurement corroborate these findings; spores recorded significantly higher adhesion force (2.92 ± 0.4 nN) compared to Vegetative Cells (0.65 ± 0.2 nN). The adhesion of spores presents greater challenges in biofilm control owing to its stronger attachment and persistence when the spores are formed under adverse environmental conditions.
Belinda Galeano - One of the best experts on this subject based on the ideXlab platform.
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inactivation of Vegetative Cells but not spores of bacillus anthracis b cereus and b subtilis on stainless steel surfaces coated with an antimicrobial silver and zinc containing zeolite formulation
Applied and Environmental Microbiology, 2003Co-Authors: Belinda Galeano, Emily Korff, Wayne L NicholsonAbstract:Stainless steel surfaces coated with paints containing a silver- and zinc-containing zeolite (AgION antimicrobial) were assayed in comparison to uncoated stainless steel for antimicrobial activity against Vegetative Cells and spores of three Bacillus species, namely, B. anthracis Sterne, B. cereus T, and B. subtilis 168. Under the test conditions (25°C and 80% relative humidity), the zeolite coating produced approximately 3 log10 inactivation of Vegetative Cells within a 5- to 24-h period, but viability of spores of the three species was not significantly affected.
Laura Wheeldon - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial efficacy of copper surfaces against spores and Vegetative Cells of clostridium difficile the germination theory
Journal of Antimicrobial Chemotherapy, 2008Co-Authors: Laura Wheeldon, Tony Worthington, Peter A Lambert, Anthony C Hilton, C J Lowden, Tom ElliottAbstract:OBJECTIVES: Persistent contamination of surfaces by spores of Clostridium difficile is a major factor influencing the spread of C. difficile-associated diarrhoea (CDAD) in the clinical setting. In recent years, the antimicrobial efficacy of metal surfaces has been investigated against microorganisms including methicillin-resistant Staphylococcus aureus. This study compared the survival of C. difficile on stainless steel, a metal contact surface widely used in hospitals, and copper surfaces. METHODS: Antimicrobial efficacy was assessed using a carrier test method against dormant spores, germinating spores and Vegetative Cells of C. difficile (NCTC 11204 and ribotype 027) over a 3 h period in the presence and absence of organic matter. RESULTS: Copper metal eliminated all Vegetative Cells of C. difficile within 30 min, compared with stainless steel which demonstrated no antimicrobial activity (P or=2.5 log reduction (99.8% reduction) at 3 h. Organic material did not reduce the antimicrobial efficacy of the copper surface (P > 0.05).
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antimicrobial efficacy of copper surfaces against spores and Vegetative Cells of clostridium difficile the germination theory
Journal of Antimicrobial Chemotherapy, 2008Co-Authors: Laura Wheeldon, Tony Worthington, Peter A Lambert, Anthony C Hilton, C J Lowden, T S J ElliottAbstract:OBJECTIVES: Persistent contamination of surfaces by spores of Clostridium difficile is a major factor influencing the spread of C. difficile-associated diarrhoea (CDAD) in the clinical setting. In recent years, the antimicrobial efficacy of metal surfaces has been investigated against microorganisms including methicillin-resistant Staphylococcus aureus. This study compared the survival of C. difficile on stainless steel, a metal contact surface widely used in hospitals, and copper surfaces. METHODS: Antimicrobial efficacy was assessed using a carrier test method against dormant spores, germinating spores and Vegetative Cells of C. difficile (NCTC 11204 and ribotype 027) over a 3 h period in the presence and absence of organic matter. RESULTS: Copper metal eliminated all Vegetative Cells of C. difficile within 30 min, compared with stainless steel which demonstrated no antimicrobial activity (P or=2.5 log reduction (99.8% reduction) at 3 h. Organic material did not reduce the antimicrobial efficacy of the copper surface (P > 0.05). CONCLUSIONS: The use of copper surfaces within the clinical environment and application of a germination solution in infection control procedures may offer a novel way forward in eliminating C. difficile from contaminated surfaces and reducing CDAD.