The Experts below are selected from a list of 4212 Experts worldwide ranked by ideXlab platform
Hermann Katinger - One of the best experts on this subject based on the ideXlab platform.
-
live cold adapted influenza a vaccine produced in Vero Cell Line
Virus Research, 2004Co-Authors: Julia Romanova, Boris Ferko, Brigitta Vcelar, Sabine Sereinig, Marina Stukova, Marjana Erofeeva, Dietmar Katinger, Oleg Kuznetsov, O. I. Kiselev, Hermann KatingerAbstract:The African green monkey kidney (Vero) Cell Line was used as a substrate for the development of a live cold-adapted (ca) reassortant influenza vaccine. For that purpose, a new master strain was generated by an adaptation of the wild type (wt) A/Singapore/1/57 virus to growth at 25 °C in a Vero Cell Line. The resulting cold-adapted (ca) muster strain A/Singapore/1/57ca showed temperature sensitive (ts) phenotype and was attenuated in animal models and protective in the challenge experiments in ferrets. Two vaccine candidates of influenza A(H1N1) and A(H3N2) subtypes (6/2 reassortants) inheriting six genes coding internal proteins from the new master strain and the surface antigens hemagglutinin (HA) and neuraminidase (NA) from the epidemic viruses were obtained by a standard method of genetic reassortment. All steps of the vaccine preparation were done exclusively in Vero Cells, including the isolation of the epidemic viruses. Both vaccine strains were used for immunization of young adult volunteers in a limited clinical trial and appeared to be safe, well tolerated and immunogenic after intranasal administration.
Julia Romanova - One of the best experts on this subject based on the ideXlab platform.
-
live cold adapted influenza a vaccine produced in Vero Cell Line
Virus Research, 2004Co-Authors: Julia Romanova, Boris Ferko, Brigitta Vcelar, Sabine Sereinig, Marina Stukova, Marjana Erofeeva, Dietmar Katinger, Oleg Kuznetsov, O. I. Kiselev, Hermann KatingerAbstract:The African green monkey kidney (Vero) Cell Line was used as a substrate for the development of a live cold-adapted (ca) reassortant influenza vaccine. For that purpose, a new master strain was generated by an adaptation of the wild type (wt) A/Singapore/1/57 virus to growth at 25 °C in a Vero Cell Line. The resulting cold-adapted (ca) muster strain A/Singapore/1/57ca showed temperature sensitive (ts) phenotype and was attenuated in animal models and protective in the challenge experiments in ferrets. Two vaccine candidates of influenza A(H1N1) and A(H3N2) subtypes (6/2 reassortants) inheriting six genes coding internal proteins from the new master strain and the surface antigens hemagglutinin (HA) and neuraminidase (NA) from the epidemic viruses were obtained by a standard method of genetic reassortment. All steps of the vaccine preparation were done exclusively in Vero Cells, including the isolation of the epidemic viruses. Both vaccine strains were used for immunization of young adult volunteers in a limited clinical trial and appeared to be safe, well tolerated and immunogenic after intranasal administration.
Christiane Bertachini Lombello - One of the best experts on this subject based on the ideXlab platform.
-
Platelet-rich plasma as supplement and scaffold for the culture of Vero Cell Line
Research on Biomedical Engineering, 2019Co-Authors: Débora Carajiliascov Ferraraz, Nelson Hidekazu Tatsui, Leonardo Ribeiro Rodrigues, Christiane Bertachini LombelloAbstract:Purpose Tissue engineering performs the culture of Cells on scaffolds, aiming at the restoration of damaged tissue. For the Cell culture into the scaffold, it is necessary to establish certain conditions, such as the type of supplementation of the culture medium. Therefore, the aim of this research was to evaluate the platelet-rich plasma (PRP) as a supplement and scaffold for Vero Cell culture, in a one case report study. Methods Calcium chloride was added to the PRP, obtained by apheresis. After PRP retraction, serum from PRP (SPRP) and the clot from PRP (CPRP) were obtained. SPRP was used as a supplement to the culture medium, and CPRP was utilized as a scaffold. Results The evaluation of SPRP as a supplement showed that there was no statistical difference in Cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero Cells cultured with SPRP. For the use of CPRP, its structure was observed. The CPRP was formed by an irregular network of fibers with different diameters, having a mean value of 0.210 ± 0.097 μ. In the analysis of CPRP as a scaffold, it was verified that the Cells adhered and spread over the biomaterial. Conclusion Therefore, in Vero Cell cultures, the results suggest that SPRP and CPRP can be used as a supplement and scaffold, respectively.
-
Platelet-rich plasma as supplement and scaffold for the culture of Vero Cell Line
Research on Biomedical Engineering, 2019Co-Authors: Débora Carajiliascov Ferraraz, Nelson Hidekazu Tatsui, Leonardo Ribeiro Rodrigues, Christiane Bertachini LombelloAbstract:Tissue engineering performs the culture of Cells on scaffolds, aiming at the restoration of damaged tissue. For the Cell culture into the scaffold, it is necessary to establish certain conditions, such as the type of supplementation of the culture medium. Therefore, the aim of this research was to evaluate the platelet-rich plasma (PRP) as a supplement and scaffold for Vero Cell culture, in a one case report study. Calcium chloride was added to the PRP, obtained by apheresis. After PRP retraction, serum from PRP (SPRP) and the clot from PRP (CPRP) were obtained. SPRP was used as a supplement to the culture medium, and CPRP was utilized as a scaffold. The evaluation of SPRP as a supplement showed that there was no statistical difference in Cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero Cells cultured with SPRP. For the use of CPRP, its structure was observed. The CPRP was formed by an irregular network of fibers with different diameters, having a mean value of 0.210 ± 0.097 μ. In the analysis of CPRP as a scaffold, it was verified that the Cells adhered and spread over the biomaterial. Therefore, in Vero Cell cultures, the results suggest that SPRP and CPRP can be used as a supplement and scaffold, respectively.
Marina Stukova - One of the best experts on this subject based on the ideXlab platform.
-
live cold adapted influenza a vaccine produced in Vero Cell Line
Virus Research, 2004Co-Authors: Julia Romanova, Boris Ferko, Brigitta Vcelar, Sabine Sereinig, Marina Stukova, Marjana Erofeeva, Dietmar Katinger, Oleg Kuznetsov, O. I. Kiselev, Hermann KatingerAbstract:The African green monkey kidney (Vero) Cell Line was used as a substrate for the development of a live cold-adapted (ca) reassortant influenza vaccine. For that purpose, a new master strain was generated by an adaptation of the wild type (wt) A/Singapore/1/57 virus to growth at 25 °C in a Vero Cell Line. The resulting cold-adapted (ca) muster strain A/Singapore/1/57ca showed temperature sensitive (ts) phenotype and was attenuated in animal models and protective in the challenge experiments in ferrets. Two vaccine candidates of influenza A(H1N1) and A(H3N2) subtypes (6/2 reassortants) inheriting six genes coding internal proteins from the new master strain and the surface antigens hemagglutinin (HA) and neuraminidase (NA) from the epidemic viruses were obtained by a standard method of genetic reassortment. All steps of the vaccine preparation were done exclusively in Vero Cells, including the isolation of the epidemic viruses. Both vaccine strains were used for immunization of young adult volunteers in a limited clinical trial and appeared to be safe, well tolerated and immunogenic after intranasal administration.
O. I. Kiselev - One of the best experts on this subject based on the ideXlab platform.
-
live cold adapted influenza a vaccine produced in Vero Cell Line
Virus Research, 2004Co-Authors: Julia Romanova, Boris Ferko, Brigitta Vcelar, Sabine Sereinig, Marina Stukova, Marjana Erofeeva, Dietmar Katinger, Oleg Kuznetsov, O. I. Kiselev, Hermann KatingerAbstract:The African green monkey kidney (Vero) Cell Line was used as a substrate for the development of a live cold-adapted (ca) reassortant influenza vaccine. For that purpose, a new master strain was generated by an adaptation of the wild type (wt) A/Singapore/1/57 virus to growth at 25 °C in a Vero Cell Line. The resulting cold-adapted (ca) muster strain A/Singapore/1/57ca showed temperature sensitive (ts) phenotype and was attenuated in animal models and protective in the challenge experiments in ferrets. Two vaccine candidates of influenza A(H1N1) and A(H3N2) subtypes (6/2 reassortants) inheriting six genes coding internal proteins from the new master strain and the surface antigens hemagglutinin (HA) and neuraminidase (NA) from the epidemic viruses were obtained by a standard method of genetic reassortment. All steps of the vaccine preparation were done exclusively in Vero Cells, including the isolation of the epidemic viruses. Both vaccine strains were used for immunization of young adult volunteers in a limited clinical trial and appeared to be safe, well tolerated and immunogenic after intranasal administration.
