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Muhammad Kasib Khan - One of the best experts on this subject based on the ideXlab platform.
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anthelmintic activity of trianthema portulacastrum l and musa paradisiaca l against gastrointestinal nematodes of sheep
Veterinary Parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Abstract Evaluation of anthelmintic effects of Trianthema ( T. ) portulacastrum L. (Aizoaceae) whole plant and Musa ( M. ) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus ( H. ) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0–8.0 g kg −1 ) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC 50 = 2.13 μg mL −1 ) was found to be more potent than T. portulacastrum (LC 50 = 2.41 μg mL −1 ) in EHT. However, in vivo , maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg −1 on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg −1 ) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus , Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible ( P T. portulacastrum and M. paradisiaca possess strong anthelmintic activity in vitro and in vivo , thus, justifying their use in the traditional Medicine system of Pakistan.
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Anthelmintic activity of Trianthema portulacastrum L. and Musa paradisiaca L. against gastrointestinal nematodes of sheep.
Veterinary parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Evaluation of anthelmintic effects of Trianthema (T.) portulacastrum L. (Aizoaceae) whole plant and Musa (M.) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus (H.) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0-8.0 g kg(-1)) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC(50)=2.13 μg mL(-1)) was found to be more potent than T. portulacastrum (LC(50)=2.41 μg mL(-1)) in EHT. However, in vivo, maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg(-1) on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg(-1)) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus, Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible (P
Altaf Hussain - One of the best experts on this subject based on the ideXlab platform.
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anthelmintic activity of trianthema portulacastrum l and musa paradisiaca l against gastrointestinal nematodes of sheep
Veterinary Parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Abstract Evaluation of anthelmintic effects of Trianthema ( T. ) portulacastrum L. (Aizoaceae) whole plant and Musa ( M. ) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus ( H. ) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0–8.0 g kg −1 ) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC 50 = 2.13 μg mL −1 ) was found to be more potent than T. portulacastrum (LC 50 = 2.41 μg mL −1 ) in EHT. However, in vivo , maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg −1 on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg −1 ) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus , Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible ( P T. portulacastrum and M. paradisiaca possess strong anthelmintic activity in vitro and in vivo , thus, justifying their use in the traditional Medicine system of Pakistan.
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Anthelmintic activity of Trianthema portulacastrum L. and Musa paradisiaca L. against gastrointestinal nematodes of sheep.
Veterinary parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Evaluation of anthelmintic effects of Trianthema (T.) portulacastrum L. (Aizoaceae) whole plant and Musa (M.) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus (H.) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0-8.0 g kg(-1)) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC(50)=2.13 μg mL(-1)) was found to be more potent than T. portulacastrum (LC(50)=2.41 μg mL(-1)) in EHT. However, in vivo, maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg(-1) on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg(-1)) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus, Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible (P
Liu Yahong - One of the best experts on this subject based on the ideXlab platform.
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Detection of PMQR Gene in Escherichia coli Isolated from Animals
2020Co-Authors: Liu YahongAbstract:【Objective】The objective of the study is to investigate the prevalence of oqxAB gene and other three types of plasmid-mediated quinolone resistance(PMQR) gene in Escherichia coli isolated from free-range farms in Guangdong province.【Method】All the strains were screened for the oqxA,oqxB,qnr,qepA,aac(6′)-Ib-cr genes by PCR.The susceptibility of PMQRpositive strains to 18 antimicrobial agents were determined by agar dilution method.【Results】The positive rates of qnrB,qnrS,aac(6′)-Ib-cr,oqxA,oqxB genes were 10.49%,18.88%,31.47%,44.8%,and 48.9% respectively.No qnrA,qnrC,qnrD and qepA genes were detected in any of the isolates.oqxA and oqxB have a high positive rate,and the two genes always exsist in the same isolates.PMQR gene always shows a coexstence of two or more kinds of the plasmid genes in the same isolates.PMQR positive isolates show a high rate of resistance to 18 antimicrobial agents which was widely used in Veterinary Clinical Medicine.【Conclusion】PMQR gene is very common in Veterinary clinic.E.coli isolated from animals in Guangdong province shows a high level resistance to widely used drugs.The antibiotic sensitivity spectrum is variegated.The proportion of multi-drug-resistant strains is high.
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Detection of Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Pet Animals
2020Co-Authors: Zhu Hengqian, Liao Xiaoping, Sun Jian, Li Liang, Zhang Meijun, Sun Ying, Chen Chaoxi, Wang Xiumei, Liu YahongAbstract:Objective) The plasmid-mediated quinolone resistance PMQR genes of qnr, qepA and aac (6')-Ib-cr in Clinical isolates of Escherichia coli from pet animals (dogs and cats) in Guangzhou city were detected. (Method) Susceptibility of 164 strains to 15 antimicrobial agents were determined by agar disc dilution method. All of the 164 strains were screened for the qnr, qepA, aac (6')-Ib-cr genes by PCR. Genetic fingerprints of the Clinical isolates were established and analyzed. (Results) The isolates showed a high rate of resistance to antimicrobial agents widely used in Veterinary Clinical Medicine and most of the them showed multiple-resistance(5 to 14 drugs). Three of these 164 specimens were detected containing qnrA, qnrB and qnrS genes, and two of which also posses aac(6')-Ib-cr gene. Fingerprints could be amplifled from all the positive strains, and strains with more than two PMQR genes were distributed mainly in the B and C gene types. (Conclusion) Escherichia coli isolated from pet animals in Guangzhou city showed a high level multiple-resistance to widely used drugs. The result of the research showed a more widespread dissemination of resistance to quinolones in pet animal Veterinary clinic of Guangzhou city, and the ability to generate PMQR gene of isolates is closely to their gene-type.
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Detection of Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Enterobacteriaceae from Avian
2020Co-Authors: Jiang Hong-xia, Liao Xiaoping, Liu Jianhua, Li Shujuan, Chen Xueying, Wu Caixia, Zhang Xiaoyun, Liu YahongAbstract:【Objective】 To detect plasmid-mediated quinolone resistance(PMQR) qnr,qepA,aac(6')-Ib-cr genes in Clinical isolates of Enterobacteriaceae from avian in Guangdong province.【Method】 Susceptibility of 84 strains to 27 antimicrobial agents were determined by agar disc diffusion method.All of the 84 strains were screened for the qnr,qepA,aac(6')-Ib-cr genes by PCR.Mutations in the quinolone-resistance-determining region(QRDRs) of the gyrA,gyrB,parC and parE gene was detected in PMQR positive strains.【Result】 The isolates showed high rate of resistance to antimicrobial agents widely used in Veterinary Clinical Medicine such as exrofloxacin,fleroxacin,ampicillin,trimethoprim-sulfamethoxazole,doxycycline,and rifampin,streptomycin and roxithromycin.All of the 84 strains showed multiple-resistance to at least 6 drugs.However most of them were sensitive to ampicillin-sulbactam,aztreonam,polymyxin E and cephalexin.A qnrB6 and aac(6')-Ib-cr gene was identified in a Klebsiella pneumoniae.Mutation of S→I occurred in the QRDR of the gyrA 83 site,while no mutations were detected in QRDRs of gyrB,parC and parE.【Conclusion】 Enterobacteriaceae isolated from avian in Guangdong showed high level multiple-resistance to widely used drugs.This is the first report on two PMQR determinants,qnr and aac(6')-Ib-cr,in Clinical isolates of K pneumoniae from avian.The emergency of PMQR genes may indicate a more widespread dissemination of resistance to quinolones in avian industry.
Muhammad Nisar Khan - One of the best experts on this subject based on the ideXlab platform.
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anthelmintic activity of trianthema portulacastrum l and musa paradisiaca l against gastrointestinal nematodes of sheep
Veterinary Parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Abstract Evaluation of anthelmintic effects of Trianthema ( T. ) portulacastrum L. (Aizoaceae) whole plant and Musa ( M. ) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus ( H. ) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0–8.0 g kg −1 ) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC 50 = 2.13 μg mL −1 ) was found to be more potent than T. portulacastrum (LC 50 = 2.41 μg mL −1 ) in EHT. However, in vivo , maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg −1 on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg −1 ) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus , Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible ( P T. portulacastrum and M. paradisiaca possess strong anthelmintic activity in vitro and in vivo , thus, justifying their use in the traditional Medicine system of Pakistan.
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Anthelmintic activity of Trianthema portulacastrum L. and Musa paradisiaca L. against gastrointestinal nematodes of sheep.
Veterinary parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Evaluation of anthelmintic effects of Trianthema (T.) portulacastrum L. (Aizoaceae) whole plant and Musa (M.) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus (H.) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0-8.0 g kg(-1)) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC(50)=2.13 μg mL(-1)) was found to be more potent than T. portulacastrum (LC(50)=2.41 μg mL(-1)) in EHT. However, in vivo, maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg(-1) on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg(-1)) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus, Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible (P
Zafar Iqbal - One of the best experts on this subject based on the ideXlab platform.
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anthelmintic activity of trianthema portulacastrum l and musa paradisiaca l against gastrointestinal nematodes of sheep
Veterinary Parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Abstract Evaluation of anthelmintic effects of Trianthema ( T. ) portulacastrum L. (Aizoaceae) whole plant and Musa ( M. ) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus ( H. ) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0–8.0 g kg −1 ) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC 50 = 2.13 μg mL −1 ) was found to be more potent than T. portulacastrum (LC 50 = 2.41 μg mL −1 ) in EHT. However, in vivo , maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg −1 on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg −1 ) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus , Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible ( P T. portulacastrum and M. paradisiaca possess strong anthelmintic activity in vitro and in vivo , thus, justifying their use in the traditional Medicine system of Pakistan.
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Anthelmintic activity of Trianthema portulacastrum L. and Musa paradisiaca L. against gastrointestinal nematodes of sheep.
Veterinary parasitology, 2011Co-Authors: Altaf Hussain, Muhammad Nisar Khan, Zafar Iqbal, Muhammad Sohail Sajid, Muhammad Kasib KhanAbstract:Evaluation of anthelmintic effects of Trianthema (T.) portulacastrum L. (Aizoaceae) whole plant and Musa (M.) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal worms of sheep was done that may justify their traditional use in Veterinary Clinical Medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME) of both the plants was determined using mature female Haemonchus (H.) contortus and their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0-8.0 g kg(-1)) was determined in sheep naturally infected with mixed species of nematodes using fecal egg count reduction test (FECRT) and larval counts. The study design also included untreated as well as treated controls. Fecal egg count reduction and larval counts from coprocultures were performed pre- and post-treatments to assess the anthelmintic activity of the plants. CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects on live worms as well as egg hatching. M. paradisiaca (LC(50)=2.13 μg mL(-1)) was found to be more potent than T. portulacastrum (LC(50)=2.41 μg mL(-1)) in EHT. However, in vivo, maximum reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg(-1) on 15th day post-treatment, respectively as compared to that of Levamisole (7.5 mg kg(-1)) that caused 97.0% reduction in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus, Trichostronglyus spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent, found susceptible (P
